Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0014942

Physical Location & Seq

Genomic Coordinates Reverse (-)

36397804 .. 36401101

Gene Structure 3298 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1308800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGGGCAGCAAGAAAGAGTTTCATACTTCTCAGAAGATTTCAATTGTTGGACCATGTTCTCTCAAGTTAAAAGAGCTTTTTGGAAAAGTAGCAAAATATGCACTGGGTTTCTTCTTAATGTATGCTCTCTGCAACGTATCCCACTTCCTGTTAAAGCCGTATTCCCAGCCTCGTATTACCACCGACACCGTTATAGGACTGCTGATCGGAAACTTAGGGTTCATACGCGACCTAGTAACGGATTCAGCCAGTAAAACGTTGAATTTCATTATTGATTTTGGCATGATCGGCTATATGTTTGTGTTAGGCATAGAAATGGATCCGTATATACTCTTTAAAGAACCAACCAAGGATGCTAAAGTGGCCTATGGTGGTATGCTCTCCACATTCATCCTAGCCTGCGCTATAACACCATTTCTGCAGTTTACAGATGGCGGTTTCAAGGTTGATTTCACTCTCTCCCTCTCCACAGTCCTCTCTAGCACCGCCTCCCCTGTACTGACACGTCTCATAACGAGTCTCAAAATAGGCAAGTCAGACATAGGCCAGCTTGTCATTTCTGCAGGAATGCACGCTGATTTCGTAGCCACTCTTCTGCTCTCAATTGGCTATGTCTTCTGGCCTGTTGAATCTGGAGAGAGAGTTTTACAGACTTCAGAACGAGTCCGAACATCCGTTGAGATGGGTTCTGCATTGGTGATCCAGACACTTTTTGCAGCCTTTGTTTCACCAATCTTCATGAACTGGGTCAACAACGAAAACCCTGAAGGCAAACCCATGAAAGGTTCACACCTAGTGCTTTCTCTTGCTTTCATGGCCTTGGTCATTGCCTGCTCGCCGATTTATGGATACAATCCTGCCTTGAGTGCATTTATGGCAGGAACCTTTTTACCAAAGGGGGGGAGGGTATCGAAATGGGTGATCGGCAAGATCAACTACTTGTTATCTACCATTTACTATCCCATTTTTTTCTTCTGGATGGGTTATGAAGCCGTGTTAAAGGAATTTCAACCCGGCCAGTTCGGGACATGGGCAAGGTTATTTGTACTTTTTGGTATAGCAACAGTTGGAAAAGTTGCTGGAACTGTCATCTGTGGAAAGTTGTTGGGATTTCAGTGGCCAGAATCTATTGCACTCGGGCTTCTTCTCACTACCAAAGGCCATTTTCACATATACTTGGCCATTGCTGCAAAACAAGCTGGGACAACGTCTACTTCGACCAGCACTGTGATGGTAATAGCTATATTTTTCACGGTTGTCCATGCACCATCAGTTGTGGCGCAAATCCTCAAGCGTGCAAGGAAACGGGCACCTACTCATCGAATGTCGCTGCAATTGCTTGACCCTTCAAGCGAACTTCGCATATTGCTATGCCTTCATGGGCCTCAAAATGTTCGTACTATGATTAACTTCATTGAGATATCTAGAGGGACGGCTGACCCTGGAATTGTGGTATATGTAACAGACGTGATAGAACTCACAGACGAAATAGAAGCCTCACTGGTAAAAGAAGAAGGGGTGGACACCATGACTGTGACTCACACAGGAGTGAAAGAAATGAGAGCTCAAGTCACCGCTGCAGTTCAAGCATATGTAGGGGAGAGCGGAGATGGTATCGCACTCAGACGGTTGCTTGCACTCTCGAATATCAATGGCATGCCCCAGGATATTTGCATTTTGGCAGAGGACTCAATGGTGGCCCTCATCATCTTGCCATTTCACAAGGTTCAACGCGCAGATGGAGCCTTGGATAGCGGAAATTCTGCTTTCAGATATGTGAACCGTAAGGTCCTCAAGAATGCTCCATGCTCCGTTGGGATTCTTGTAGACAGAGGCCTTGGACTGATTGAAAAACTACCAAGAACTTACGACCATTCTGTCAGCGTGGCAGTCATTTTCATTGGTGGAAAAGACGACAGAGAAGCACTTGCCTACGCAGGTCGCGTAGCACGCCATGCAGGTGTAAAGCTCACAGTCATAAGATTCTTGGTAGATGCTAACTCAGAGGCAGCAGCCAGACCTGGAAATTACACAGTCAGCCGTTCTGAGCAAGAAGAGGAAATGAGGCTTGACGATGAGTGTTTCGCAAGCTTCTACGAGAGACATGTCGCGTGTGGACAAGTTGCGTACATGGAGAAGCATATGGCCAATTCTGCGGAGACGTATTCTACCTTGAAGGCATTGGACGGGCAGTACGCGCTCATTATAGTGGGACGAGGGGAAAGGGTGAATTCGGTTCTGACATTCGGGATGAATGACTGGCAGCAATGTCCGGAGTTGGGGCCGATCGGAGATGTCCTTTCGGGTTCTGATTTCTCGGTGAGAACCTCGGTTTTGATCATCCAAGAAAACAGTCTAAAAGGACAACTAGATGGGCTGGAAGATGACTTTTCGATCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0016020 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

801

Amino Acids

87.41

Weight (kDa)

7.22

Isoelectric Point (pI)

30.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	44 - 431	2.3e-35	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	484 - 614	2.1e-15	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	626 - 784	1.4e-14	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014942)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52080
fragaria_vesca	FvH4_7g01210 FvH4_7g01210
malus_domestica	MD02G1308800.v1.1 MD07G1013200.v1.1
prunus_persica	Prupe.2G016700_v2.0.a1 Prupe.2G016700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0316681
rosa_laevigata	RLG00000030644
rosa_multiflora	Rmu_sc0005539.1_g000005
rosa_roxburghii	Rroxscaffold_4G00330860
rosa_samantha	Rh1AG022700 Rh1BG021200 Rh1CG019800 Rh1DG017200
rosa_wichuraiana	Rw1G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1949
AasI	GACNNNNNNGTC	1 cut(s)	1877
Acc36I	ACCTGC	2 cut(s)	1928, 1949
AccB1I	GGYRCC	1 cut(s)	1309
AccBSI	CCGCTC	1 cut(s)	1605
AccI	GTMKAC	2 cut(s)	1211, 1827
AccII	CGCG	5 cut(s)	228, 1734, 1944, 2111, 2198
AccIII	TCCGGA	1 cut(s)	2272
AciI	CCGC	6 cut(s)	435, 486, 1575, 1605, 1755, 2156
AclI	AACGTT	1 cut(s)	257
AclWI	GGATC	3 cut(s)	314, 327, 694
AcoI	YGGCCR	4 cut(s)	1015, 1118, 1179, 2145
AcsI	RAATTY	4 cut(s)	262, 1004, 1759, 2230
AcuI	CTGAAG	2 cut(s)	639, 786
AdeI	CACNNNGTG	1 cut(s)	796
AfaI	GTAC	5 cut(s)	498, 1047, 1399, 2129, 2195
AfiI	CCNNNNNNNGG	5 cut(s)	238, 782, 845, 899, 2279
AflIII	ACRYGT	2 cut(s)	503, 2104
AjiI	CACGTC	2 cut(s)	506, 1468
AjnI	CCWGG	3 cut(s)	1441, 1662, 2020
AleI	CACNNNNGTG	1 cut(s)	1547
AluBI	AGCT	7 cut(s)	76, 550, 1199, 1241, 1565, 1969, 2091
AluI	AGCT	7 cut(s)	76, 550, 1199, 1241, 1565, 1969, 2091
Alw21I	GWGCWC	1 cut(s)	1567
Alw26I	GTCTC	4 cut(s)	512, 524, 2095, 2153
AlwI	GGATC	3 cut(s)	314, 327, 694
Ama87I	CYCGRG	1 cut(s)	1136
Aor13HI	TCCGGA	1 cut(s)	2272
ApeKI	GCWGC	8 cut(s)	6, 716, 1187, 1330, 1577, 2009, 2012, 2263
ApoI	RAATTY	4 cut(s)	262, 1004, 1759, 2230
AspLEI	GCGC	4 cut(s)	404, 1282, 1736, 2200
AspS9I	GGNCC	5 cut(s)	50, 1382, 1699, 1789, 2282
AsuC2I	CCSGG	1 cut(s)	1014
AsuHPI	GGTGA	6 cut(s)	709, 720, 931, 1564, 2239, 2332
AvaI	CYCGRG	1 cut(s)	1136
AvaII	GGWCC	2 cut(s)	50, 1789
BaeGI	GKGCMC	1 cut(s)	1312
BalI	TGGCCA	3 cut(s)	1120, 1181, 2147
BamHI	GGATCC	1 cut(s)	319
BanI	GGYRCC	1 cut(s)	1309
BanII	GRGCYC	1 cut(s)	1567
BarI	GAAGNNNNNNTAC	2 cut(s)	576, 608
BbsI	GAAGAC	1 cut(s)	607
Bbv12I	GWGCWC	1 cut(s)	1567
BbvI	GCAGC	8 cut(s)	18, 728, 1174, 1317, 1564, 2021, 2024, 2275
BccI	CCATC	8 cut(s)	425, 676, 973, 1225, 1276, 1604, 1733, 2366
BceAI	ACGGC	4 cut(s)	142, 977, 1449, 2025
BciT130I	CCWGG	3 cut(s)	1443, 1664, 2022
BciVI	GTATCC	2 cut(s)	148, 842
BclI	TGATCA	1 cut(s)	2337
BcnI	CCSGG	1 cut(s)	1014
BcoDI	GTCTC	4 cut(s)	512, 524, 2095, 2153
BfaI	CTAG	6 cut(s)	233, 395, 480, 794, 1425, 2369
BfmI	CTRYAG	3 cut(s)	419, 561, 1578
BfuAI	ACCTGC	2 cut(s)	1928, 1949
BfuI	GTATCC	2 cut(s)	148, 842
BisI	GCNGC	8 cut(s)	7, 717, 1188, 1331, 1578, 2010, 2013, 2264
BlsI	GCNGC	8 cut(s)	8, 718, 1189, 1332, 1579, 2011, 2014, 2265
Bme1390I	CCNGG	4 cut(s)	1014, 1443, 1664, 2022
Bme18I	GGWCC	2 cut(s)	50, 1789
BmeT110I	CYCGRG	1 cut(s)	1136
BmgBI	CACGTC	2 cut(s)	506, 1468
BmgT120I	GGNCC	5 cut(s)	50, 1382, 1699, 1789, 2282
BmiI	GGNNCC	5 cut(s)	321, 883, 1311, 1744, 2283
BmrFI	CCNGG	4 cut(s)	1014, 1443, 1664, 2022
BmrI	ACTGGG	2 cut(s)	113, 754
BmsI	GCATC	2 cut(s)	343, 1984
BmuI	ACTGGG	2 cut(s)	113, 754
BpiI	GAAGAC	1 cut(s)	607
BpmI	CTGGAG	1 cut(s)	654
BpuEI	CTTGAG	5 cut(s)	47, 883, 1274, 1551, 1778
BpuMI	CCSGG	1 cut(s)	1014
BsaJI	CCNNGG	7 cut(s)	348, 819, 1441, 1662, 1746, 1837, 2328
BsaWI	WCCGGW	1 cut(s)	2272
BsaXI	ACNNNNNCTCC	2 cut(s)	627, 657
Bsc4I	CCNNNNNNNGG	5 cut(s)	238, 782, 845, 899, 2279
Bse1I	ACTGG	6 cut(s)	108, 249, 749, 1018, 1506, 2264
Bse3DI	GCAATG	3 cut(s)	825, 1182, 2273
BseAI	TCCGGA	1 cut(s)	2272
BseBI	CCWGG	3 cut(s)	1443, 1664, 2022
BseDI	CCNNGG	7 cut(s)	348, 819, 1441, 1662, 1746, 1837, 2328
BseGI	GGATG	6 cut(s)	358, 390, 671, 984, 2256, 2340
BseLI	CCNNNNNNNGG	5 cut(s)	238, 782, 845, 899, 2279
BseMI	GCAATG	3 cut(s)	825, 1182, 2273
BseMII	CTCAG	4 cut(s)	44, 1636, 2016, 2037
BseNI	ACTGG	6 cut(s)	108, 249, 749, 1018, 1506, 2264
BseSI	GKGCMC	1 cut(s)	1312
BseXI	GCAGC	8 cut(s)	18, 728, 1174, 1317, 1564, 2021, 2024, 2275
BseYI	CCCAGC	2 cut(s)	165, 1199
Bsh1236I	CGCG	5 cut(s)	228, 1734, 1944, 2111, 2198
Bsh1285I	CGRYCG	1 cut(s)	2289
BshNI	GGYRCC	1 cut(s)	1309
BsiEI	CGRYCG	1 cut(s)	2289
BsiHKAI	GWGCWC	1 cut(s)	1567
BsiHKCI	CYCGRG	1 cut(s)	1136
BsiSI	CCGG	2 cut(s)	1014, 2273
BslFI	GGGAC	4 cut(s)	1039, 1216, 1444, 2226
BslI	CCNNNNNNNGG	5 cut(s)	238, 782, 845, 899, 2279
BsmAI	GTCTC	4 cut(s)	512, 524, 2095, 2153
BsmBI	CGTCTC	2 cut(s)	512, 2153
BsmFI	GGGAC	4 cut(s)	1039, 1216, 1444, 2226
BsmI	GAATGC	2 cut(s)	573, 1804
BsoBI	CYCGRG	1 cut(s)	1136
Bsp1286I	GDGCHC	2 cut(s)	1312, 1567
Bsp13I	TCCGGA	1 cut(s)	2272
Bsp143I	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
BspACI	CCGC	6 cut(s)	435, 486, 1575, 1605, 1755, 2156
BspCNI	CTCAG	4 cut(s)	43, 1635, 2015, 2038
BspEI	TCCGGA	1 cut(s)	2272
BspFNI	CGCG	5 cut(s)	228, 1734, 1944, 2111, 2198
BspHI	TCATGA	1 cut(s)	738
BspLI	GGNNCC	5 cut(s)	321, 883, 1311, 1744, 2283
BspMAI	CTGCAG	3 cut(s)	423, 565, 1582
BspMI	ACCTGC	2 cut(s)	1928, 1949
BspPI	GGATC	3 cut(s)	314, 327, 694
BspT107I	GGYRCC	1 cut(s)	1309
BsrBI	CCGCTC	1 cut(s)	1605
BsrDI	GCAATG	3 cut(s)	825, 1182, 2273
BsrI	ACTGG	6 cut(s)	108, 249, 749, 1018, 1506, 2264
BssECI	CCNNGG	7 cut(s)	348, 819, 1441, 1662, 1746, 1837, 2328
BssMI	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
BssT1I	CCWWGG	4 cut(s)	348, 819, 1746, 1837
Bst2UI	CCWGG	3 cut(s)	1443, 1664, 2022
Bst6I	CTCTTC	2 cut(s)	597, 2049
BstAPI	GCANNNNNTGC	2 cut(s)	98, 1955
BstDEI	CTNAG	5 cut(s)	30, 214, 1622, 2002, 2046
BstF5I	GGATG	6 cut(s)	358, 390, 671, 984, 2256, 2340
BstFNI	CGCG	5 cut(s)	228, 1734, 1944, 2111, 2198
BstHHI	GCGC	4 cut(s)	404, 1282, 1736, 2200
BstKTI	GATC	9 cut(s)	207, 288, 322, 702, 924, 933, 2289, 2340, 2397
BstMAI	GTCTC	4 cut(s)	512, 524, 2095, 2153
BstMBI	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
BstMCI	CGRYCG	1 cut(s)	2289
BstNI	CCWGG	3 cut(s)	1443, 1664, 2022
BstNSI	RCATGY	2 cut(s)	1660, 2108
BstSCI	CCNGG	4 cut(s)	1012, 1441, 1662, 2020
BstSFI	CTRYAG	3 cut(s)	419, 561, 1578
BstSLI	GKGCMC	1 cut(s)	1312
BstUI	CGCG	5 cut(s)	228, 1734, 1944, 2111, 2198
BstV1I	GCAGC	8 cut(s)	18, 728, 1174, 1317, 1564, 2021, 2024, 2275
BstV2I	GAAGAC	1 cut(s)	607
BstX2I	RGATCY	1 cut(s)	319
BstYI	RGATCY	1 cut(s)	319
BsuI	GTATCC	2 cut(s)	148, 842
BtrI	CACGTC	2 cut(s)	506, 1468
BtsCI	GGATG	6 cut(s)	358, 390, 671, 984, 2256, 2340
BtsIMutI	CAGTG	4 cut(s)	101, 1121, 1224, 1499
BveI	ACCTGC	2 cut(s)	1928, 1949
CciI	TCATGA	1 cut(s)	738
CfoI	GCGC	4 cut(s)	404, 1282, 1736, 2200
Cfr13I	GGNCC	5 cut(s)	50, 1382, 1699, 1789, 2282
Csp6I	GTAC	5 cut(s)	497, 1046, 1398, 2128, 2194
CviQI	GTAC	5 cut(s)	497, 1046, 1398, 2128, 2194
DdeI	CTNAG	5 cut(s)	30, 214, 1622, 2002, 2046
DpnI	GATC	9 cut(s)	206, 287, 321, 701, 923, 932, 2288, 2339, 2396
DpnII	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
DraI	TTTAAA	1 cut(s)	337
DraIII	CACNNNGTG	1 cut(s)	796
DrdI	GACNNNNNNGTC	1 cut(s)	1877
DseDI	GACNNNNNNGTC	1 cut(s)	1877
EaeI	YGGCCR	4 cut(s)	1015, 1118, 1179, 2145
Eam1104I	CTCTTC	2 cut(s)	597, 2049
EarI	CTCTTC	2 cut(s)	597, 2049
Ecl136II	GAGCTC	1 cut(s)	1565
Eco130I	CCWWGG	4 cut(s)	348, 819, 1746, 1837
Eco147I	AGGCCT	1 cut(s)	1836
Eco24I	GRGCYC	1 cut(s)	1567
Eco32I	GATATC	1 cut(s)	1422
Eco47I	GGWCC	2 cut(s)	50, 1789
Eco53kI	GAGCTC	1 cut(s)	1565
Eco57I	CTGAAG	2 cut(s)	639, 786
Eco88I	CYCGRG	1 cut(s)	1136
EcoICRI	GAGCTC	1 cut(s)	1565
EcoO109I	RGGNCCY	1 cut(s)	1789
EcoRI	GAATTC	1 cut(s)	2230
EcoRII	CCWGG	3 cut(s)	1441, 1662, 2020
EcoRV	GATATC	1 cut(s)	1422
EcoT14I	CCWWGG	4 cut(s)	348, 819, 1746, 1837
EcoT38I	GRGCYC	1 cut(s)	1567
ErhI	CCWWGG	4 cut(s)	348, 819, 1746, 1837
Esp3I	CGTCTC	2 cut(s)	512, 2153
FaqI	GGGAC	4 cut(s)	1039, 1216, 1444, 2226
FauNDI	CATATG	2 cut(s)	1591, 2142
FbaI	TGATCA	1 cut(s)	2337
FblI	GTMKAC	2 cut(s)	1211, 1827
Fnu4HI	GCNGC	8 cut(s)	7, 717, 1188, 1331, 1578, 2010, 2013, 2264
FokI	GGATG	6 cut(s)	365, 377, 658, 991, 2263, 2327
FriOI	GRGCYC	1 cut(s)	1567
Fsp4HI	GCNGC	8 cut(s)	7, 717, 1188, 1331, 1578, 2010, 2013, 2264
FspBI	CTAG	6 cut(s)	233, 395, 480, 794, 1425, 2369
GlaI	GCGC	4 cut(s)	403, 1281, 1735, 2199
GluI	GCNGC	8 cut(s)	7, 717, 1188, 1331, 1578, 2010, 2013, 2264
GsaI	CCCAGC	2 cut(s)	169, 1203
GsuI	CTGGAG	1 cut(s)	654
HapII	CCGG	2 cut(s)	1014, 2273
HhaI	GCGC	4 cut(s)	404, 1282, 1736, 2200
Hin6I	GCGC	4 cut(s)	402, 1280, 1734, 2198
HinP1I	GCGC	4 cut(s)	402, 1280, 1734, 2198
HincII	GTYRAC	1 cut(s)	751
HindII	GTYRAC	1 cut(s)	751
HindIII	AAGCTT	1 cut(s)	2089
HinfI	GANTC	9 cut(s)	242, 517, 629, 663, 1124, 1537, 1688, 1819, 1983
HpaII	CCGG	2 cut(s)	1014, 2273
HphI	GGTGA	6 cut(s)	709, 720, 931, 1564, 2239, 2332
Hpy166II	GTNNAC	8 cut(s)	426, 751, 788, 1212, 1522, 1780, 1828, 2117
Hpy8I	GTNNAC	8 cut(s)	426, 751, 788, 1212, 1522, 1780, 1828, 2117
HpyAV	CCTTC	5 cut(s)	761, 1356, 1385, 1508, 2170
HpyCH4IV	ACGT	6 cut(s)	135, 257, 505, 1208, 1467, 2162
HpyF3I	CTNAG	5 cut(s)	30, 214, 1622, 2002, 2046
HpySE526I	ACGT	6 cut(s)	135, 257, 505, 1208, 1467, 2162
HspAI	GCGC	4 cut(s)	402, 1280, 1734, 2198
Kpn2I	TCCGGA	1 cut(s)	2272
Ksp22I	TGATCA	1 cut(s)	2337
Kzo9I	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
LmnI	GCTCC	3 cut(s)	1742, 1807, 1814
Lsp1109I	GCAGC	8 cut(s)	18, 728, 1174, 1317, 1564, 2021, 2024, 2275
LweI	GCATC	2 cut(s)	343, 1984
MaeI	CTAG	6 cut(s)	233, 395, 480, 794, 1425, 2369
MaeII	ACGT	6 cut(s)	135, 257, 505, 1208, 1467, 2162
MaeIII	GTNAC	4 cut(s)	235, 1459, 1534, 1570
MalI	GATC	9 cut(s)	206, 287, 321, 701, 923, 932, 2288, 2339, 2396
MbiI	CCGCTC	1 cut(s)	1605
MboI	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
MfeI	CAATTG	3 cut(s)	42, 603, 1334
MflI	RGATCY	1 cut(s)	319
MhlI	GDGCHC	2 cut(s)	1312, 1567
MlsI	TGGCCA	3 cut(s)	1120, 1181, 2147
MluNI	TGGCCA	3 cut(s)	1120, 1181, 2147
MlyI	GAGTC	4 cut(s)	526, 672, 1531, 1682
MmeI	TCCRAC	2 cut(s)	28, 1048
Mox20I	TGGCCA	3 cut(s)	1120, 1181, 2147
MroI	TCCGGA	1 cut(s)	2272
MscI	TGGCCA	3 cut(s)	1120, 1181, 2147
MseI	TTAA	6 cut(s)	68, 116, 152, 336, 998, 1407
MslI	CAYNNNNRTG	6 cut(s)	314, 1229, 1532, 1547, 1959, 2207
Msp20I	TGGCCA	3 cut(s)	1120, 1181, 2147
MspA1I	CMGCKG	1 cut(s)	1577
MspI	CCGG	2 cut(s)	1014, 2273
MspR9I	CCNGG	4 cut(s)	1014, 1443, 1664, 2022
MunI	CAATTG	3 cut(s)	42, 603, 1334
Mva1269I	GAATGC	2 cut(s)	573, 1804
MvaI	CCWGG	3 cut(s)	1443, 1664, 2022
MvnI	CGCG	5 cut(s)	228, 1734, 1944, 2111, 2198
NciI	CCSGG	1 cut(s)	1014
NdeI	CATATG	2 cut(s)	1591, 2142
NdeII	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
NlaIV	GGNNCC	5 cut(s)	321, 883, 1311, 1744, 2283
NmuCI	GTSAC	2 cut(s)	1534, 1570
NspI	RCATGY	2 cut(s)	1660, 2108
OliI	CACNNNNGTG	1 cut(s)	1547
PaeI	GCATGC	1 cut(s)	1660
PagI	TCATGA	1 cut(s)	738
PaqCI	CACCTGC	1 cut(s)	1949
PceI	AGGCCT	1 cut(s)	1836
PciI	ACATGT	1 cut(s)	2104
PcsI	WCGNNNNNNNCGW	3 cut(s)	579, 1214, 1941
PctI	GAATGC	2 cut(s)	573, 1804
PfeI	GAWTC	5 cut(s)	242, 629, 1124, 1819, 1983
PflFI	GACNNNGTC	1 cut(s)	1207
PkrI	GCNGC	8 cut(s)	8, 718, 1189, 1332, 1579, 2011, 2014, 2265
Ple19I	CGATCG	1 cut(s)	2289
PleI	GAGTC	4 cut(s)	525, 671, 1531, 1682
PpsI	GAGTC	4 cut(s)	525, 671, 1531, 1682
PpuMI	RGGWCCY	1 cut(s)	1789
PscI	ACATGT	1 cut(s)	2104
Psp124BI	GAGCTC	1 cut(s)	1567
Psp1406I	AACGTT	1 cut(s)	257
Psp5II	RGGWCCY	1 cut(s)	1789
Psp6I	CCWGG	3 cut(s)	1441, 1662, 2020
PspFI	CCCAGC	2 cut(s)	165, 1199
PspGI	CCWGG	3 cut(s)	1441, 1662, 2020
PspN4I	GGNNCC	5 cut(s)	321, 883, 1311, 1744, 2283
PspPI	GGNCC	5 cut(s)	50, 1382, 1699, 1789, 2282
PspPPI	RGGWCCY	1 cut(s)	1789
PstI	CTGCAG	3 cut(s)	423, 565, 1582
PsuI	RGATCY	1 cut(s)	319
PsyI	GACNNNGTC	1 cut(s)	1207
PvuI	CGATCG	1 cut(s)	2289
RsaI	GTAC	5 cut(s)	498, 1047, 1399, 2129, 2195
RsaNI	GTAC	5 cut(s)	497, 1046, 1398, 2128, 2194
RseI	CAYNNNNRTG	6 cut(s)	314, 1229, 1532, 1547, 1959, 2207
SacI	GAGCTC	1 cut(s)	1567
SaqAI	TTAA	6 cut(s)	68, 116, 152, 336, 998, 1407
SatI	GCNGC	8 cut(s)	7, 717, 1188, 1331, 1578, 2010, 2013, 2264
Sau3AI	GATC	9 cut(s)	204, 285, 319, 699, 921, 930, 2286, 2337, 2394
Sau96I	GGNCC	5 cut(s)	50, 1382, 1699, 1789, 2282
SchI	GAGTC	4 cut(s)	526, 672, 1531, 1682
ScrFI	CCNGG	4 cut(s)	1014, 1443, 1664, 2022
SduI	GDGCHC	2 cut(s)	1312, 1567
SfaNI	GCATC	2 cut(s)	343, 1984
SfcI	CTRYAG	3 cut(s)	419, 561, 1578
SinI	GGWCC	2 cut(s)	50, 1789
SmiMI	CAYNNNNRTG	6 cut(s)	314, 1229, 1532, 1547, 1959, 2207
SmlI	CTYRAG	5 cut(s)	62, 862, 1289, 1566, 1793
SmoI	CTYRAG	5 cut(s)	62, 862, 1289, 1566, 1793
SphI	GCATGC	1 cut(s)	1660
SseBI	AGGCCT	1 cut(s)	1836
SsiI	CCGC	6 cut(s)	435, 486, 1575, 1605, 1755, 2156
SspMI	CTAG	6 cut(s)	233, 395, 480, 794, 1425, 2369
SstI	GAGCTC	1 cut(s)	1567
StuI	AGGCCT	1 cut(s)	1836
StyD4I	CCNGG	4 cut(s)	1012, 1441, 1662, 2020
StyI	CCWWGG	4 cut(s)	348, 819, 1746, 1837
TaiI	ACGT	6 cut(s)	138, 260, 508, 1211, 1470, 2165
TaqI	TCGA	5 cut(s)	911, 1217, 1321, 1643, 2393
TatI	WGTACW	2 cut(s)	496, 1045
TfiI	GAWTC	5 cut(s)	242, 629, 1124, 1819, 1983
Tru1I	TTAA	6 cut(s)	68, 116, 152, 336, 998, 1407
Tru9I	TTAA	6 cut(s)	68, 116, 152, 336, 998, 1407
TscAI	CASTG	4 cut(s)	108, 1121, 1231, 1506
TseFI	GTSAC	2 cut(s)	1534, 1570
TseI	GCWGC	8 cut(s)	6, 716, 1187, 1330, 1577, 2009, 2012, 2263
Tsp45I	GTSAC	2 cut(s)	1534, 1570
TspGWI	ACGGA	4 cut(s)	254, 312, 664, 1801
TspRI	CASTG	4 cut(s)	108, 1121, 1231, 1506
Tth111I	GACNNNGTC	1 cut(s)	1207
VpaK11BI	GGWCC	2 cut(s)	50, 1789
XapI	RAATTY	4 cut(s)	262, 1004, 1759, 2230
XbaI	TCTAGA	1 cut(s)	1424
XceI	RCATGY	2 cut(s)	1660, 2108
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1228
XmiI	GTMKAC	2 cut(s)	1211, 1827
XspI	CTAG	6 cut(s)	233, 395, 480, 794, 1425, 2369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.