Rh1DG017200

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014942
Physical Location & Seq
Reverse (-)
2833460 .. 2838313
4854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG017200.1

Sequence Viewer

Length: 2382 bp
ATGGAGGAGAGCAACAAAAAAATTCACAGTGTTGATAGAATTGACATTCCTAAAGGATGCTCATTGCAGTTACAAAAACTTTTCACAGATGTAGCCCTTCATGTTGGAGGTTTCTTCTTAATGTACATCCTGTGTCATCTTTCGCACTACCTGCTAAGGCCCTATTCACAGCCTCGCATTACCTCTGATACCTTTGTAGGACTAGTCATCGGAAACATAGGGTTCATACGCAATATTCTATCAAAGCGAGTATTGCAGTACATAGTTGATTTTGGAATGATTGGCTACATGTTTGTGCTGGGAATAGAAATGGATCCATATGTGCTTGTTAGAGCACCAACCAAAGACGCGAAAGTAGCCTATGCTGGTATGCTCTTCACATTCGCCCTAGCCTGTGGAATAACCCCATTTCTGAACTATACAGACGGTTTTAAGATTGACTTCACTCTCTCCCTCTCCATGGTCCTCTCTAGCACGGCTTCGCCTGTACTGACACGAATGATAACAAATCTCAAAATAGGCAAGTCTGACATAGGCCAGCTTGTCATTGCTACTGGAATGCATTCTGATTTTATAGCCACTCTTCTCCTCTCCCTTGGCTATGTCATTTGGCCTGTTCAAGTTGAAGATAAAAAGCCCAATGTTCGCAGATCCCTTCAGATGGGTGCTGCATTGTTGGTCCAGACAATTTTTACAGCCGTTGTTTCACCAGTCGTCATGAACTGGGTCAACAATGAAAACCCCGAAGGCAAACCCATCAAAGGTTCACACCTAGTCCTCTCTATGGCTTTCATGGCCTTTGTTGTGGCTTGCCCATCATTTTGGGGGTACAGTGCTATTTTGAGTGCATTTATGGCAGGAATCTTTCTACCAAGGGAAGGAAGAGTATCAAAGTGGGTAATAGGCAAGATCAATTACTTGTTAAGTACCATATTCTATCCCATATTTTTCTTTTGGATGGGGTATGAAGCTTATTTTATGGAGTTTCAACCTCTTGACTGGATGACATGGGCAAGGTTATTTGTAGTTTTTGCTATAGCAACAATCGGAAAAGTTGCTGGAACAGTGGTCTCTGGAGCGTTGTTGGGGTTTAACTGGCCTGAATCTGTTGCACTAGGGCTTCTTCTCACTACCAAAGGCCATTTTCATATATACTTGGCTATTGCTGCAAAATCGTATGGGAAAACCACTACTTCAACCAGCACTGTGATGGTAATAGCTATTTTTTTTACTGTGGTGCACGCCCCATCAGTTGTGGCTCAAATTATTAAGCGTGCAAGGAGACGGGCACCCACTCATCGGATGTCACTTCAGTTGCTTGAACCTTCAAGCGAACTCCGCATATTGCTATGCCTTCATGGGCCACAAAATTTGCGTACAACCATTAACTTCATTGAGATATGTAGAGGGACAGCAGACCCTGGAATCGTGGTATTTGTAACAGACATGATAGAACTCACAGACCAAATAGCAGCCACACTAGTTAAAGAAGAAGGAGTGAACACAATGACTGTGACTGACAAGGCAGTCACAGACAGGAGAGATCAAGTCACTGCTGCAGTTCAAGCCTATGTAGAGGAGGATGAAACTGGTATTACACTTGGACGATTGCTTGCACTTTCGACTTTCAATGGCATGCCCCAAGATATATGCATTTTGGCTGAGGACTCGATGGTGGCCCTCATCATATTGCCGTTTCACAAGACACAAGGTGCAAATGGGACATTAGATGGCGGCCATTCTGGTTTCAGATATGTGAACCGTAAGGTCCTCAGAAATGCTCCATGCTCCGTAGGAATTCTTGTAGACAGAGGTTTTGGACTGATTGAAAAAATATCAAGATCTCATGATCATTCTATCGATATGGCAGTCATCTTCATTGGTGGAAAAGATGATAGAGAAGCACTTGCTTATGCCGGTCATGTAGCACAACATCCTGGTGTAAAACTCACCATCTTAAGATTTTTGGTAGACAGTAGCTCGGATGCCTCAAGTAGACCTAGCCATTATCAGGTCAGTCGTGCTCTACAAGAAGAGGAAATGAAGCTTGATGATGAGTGCTTTGCTAAGTTCTACGAGAGACATGTTGCAATTGGGCAAGTTGCTTACATGGAGAAGCATCTTGCCAATTCTTCTGAGACCTATAATACCTTGATGACATTGGATGGGCAGTACTCACTTATTATAGTGGGAAGAGGGGAAAGGGTGAATACAGTTTTGACATTTGGGATGAATGACTGGCAGAAATGTCCGGAGTTGGGTCCGATCGGTGATGTTCTTTCAGGTTCTGAATTTTCAGTCAGAACCTCAGTTTTGATCATCCAACAAAACAGTGGAAAAGGAGAACTAGATGGACTTGATGATGACTTTTCTGTCATGTAA

Protein Analysis

793

Amino Acids

87.71

Weight (kDa)

6.71

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 44 - 423 5.9e-37 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 476 - 613 3.7e-14 Plant cation/H(+) antiporter domain
CHX17_C PF23259 619 - 780 1e-15 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014942)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52080
fragaria_vesca FvH4_7g01210 FvH4_7g01210
malus_domestica MD02G1308800.v1.1 MD07G1013200.v1.1
prunus_persica Prupe.2G016700_v2.0.a1 Prupe.2G016700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0316681
rosa_laevigata RLG00000030644
rosa_multiflora Rmu_sc0005539.1_g000005
rosa_roxburghii Rroxscaffold_4G00330860
rosa_samantha Rh1AG022700 Rh1BG021200 Rh1CG019800 Rh1DG017200
rosa_wichuraiana Rw1G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1526, 2372
Acc36I ACCTGC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 1288
AccI GTMKAC 3 cut(s) 1806, 1971, 1996
AccII CGCG 1 cut(s) 350
AccIII TCCGGA 1 cut(s) 2251
AciI CCGC 2 cut(s) 1339, 1734
AclWI GGATC 3 cut(s) 308, 321, 645
AcoI YGGCCR 1 cut(s) 1735
AcsI RAATTY 4 cut(s) 21, 1369, 1797, 2291
AcuI CTGAAG 2 cut(s) 641, 1295
AdeI CACNNNGTG 1 cut(s) 1712
AfaI GTAC 7 cut(s) 125, 260, 489, 830, 928, 1378, 2174
AfiI CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2011, 2258
AflII CTTAAG 1 cut(s) 1957
AflIII ACRYGT 2 cut(s) 288, 2083
AgsI TTSAA 9 cut(s) 620, 626, 989, 1197, 1322, 1329, 1565, 1630, 1829
AhlI ACTAGT 2 cut(s) 202, 1480
AjnI CCWGG 2 cut(s) 1420, 1936
AluBI AGCT 5 cut(s) 541, 971, 1220, 1980, 2047
AluI AGCT 5 cut(s) 541, 971, 1220, 1980, 2047
Alw21I GWGCWC 3 cut(s) 337, 1242, 2026
Alw26I GTCTC 4 cut(s) 1075, 1276, 2074, 2132
Alw44I GTGCAC 1 cut(s) 1238
AlwI GGATC 3 cut(s) 308, 321, 645
AlwNI CAGNNNCTG 2 cut(s) 1421, 2288
Aor13HI TCCGGA 1 cut(s) 2251
AoxI GGCC 9 cut(s) 158, 535, 611, 795, 1097, 1138, 1361, 1677, 1735
ApaLI GTGCAC 1 cut(s) 1238
ApeKI GCWGC 4 cut(s) 668, 1166, 1472, 1556
ApoI RAATTY 4 cut(s) 21, 1369, 1797, 2291
ArsI GACNNNNNNTTYG 2 cut(s) 1799, 1831
Asp700I GAANNNNTTC 1 cut(s) 562
AspS9I GGNCC 7 cut(s) 159, 463, 679, 1361, 1678, 1768, 2261
AsuHPI GGTGA 4 cut(s) 699, 1942, 2218, 2282
AvaII GGWCC 4 cut(s) 463, 679, 1768, 2261
BaeGI GKGCMC 2 cut(s) 1242, 1291
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 1288
Bbv12I GWGCWC 3 cut(s) 337, 1242, 2026
BbvCI CCTCAGC 1 cut(s) 1662
BbvI GCAGC 4 cut(s) 655, 1153, 1484, 1543
BceAI ACGGC 3 cut(s) 492, 683, 1678
BciT130I CCWGG 2 cut(s) 1422, 1938
BclI TGATCA 2 cut(s) 1849, 2316
BcoDI GTCTC 4 cut(s) 1075, 1276, 2074, 2132
BcuI ACTAGT 2 cut(s) 202, 1480
BfaI CTAG 8 cut(s) 203, 389, 471, 773, 1115, 1481, 2001, 2348
BfmI CTRYAG 2 cut(s) 1035, 1557
BfrI CTTAAG 1 cut(s) 1957
BfuAI ACCTGC 1 cut(s) 159
BglII AGATCT 1 cut(s) 1841
BisI GCNGC 5 cut(s) 669, 1167, 1473, 1557, 1735
BlsI GCNGC 5 cut(s) 670, 1168, 1474, 1558, 1736
BmcAI AGTACT 1 cut(s) 2174
Bme1390I CCNGG 2 cut(s) 1422, 1938
Bme18I GGWCC 4 cut(s) 463, 679, 1768, 2261
BmgT120I GGNCC 7 cut(s) 159, 463, 679, 1361, 1678, 1768, 2261
BmiI GGNNCC 3 cut(s) 315, 1290, 2262
BmrFI CCNGG 2 cut(s) 1422, 1938
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 3 cut(s) 47, 1975, 2128
BmuI ACTGGG 1 cut(s) 733
BpmI CTGGAG 1 cut(s) 1095
Bpu10I CCTNAGC 2 cut(s) 155, 1662
BpuEI CTTGAG 1 cut(s) 1975
Bsa29I ATCGAT 1 cut(s) 1860
BsaI GGTCTC 2 cut(s) 1075, 2132
BsaJI CCNNGG 4 cut(s) 459, 595, 872, 1420
BsaWI WCCGGW 1 cut(s) 2251
Bsc4I CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2011, 2258
Bse118I RCCGGY 1 cut(s) 1916
Bse1I ACTGG 7 cut(s) 559, 710, 728, 1004, 1100, 1594, 2243
Bse3DI GCAATG 2 cut(s) 62, 546
BseAI TCCGGA 1 cut(s) 2251
BseBI CCWGG 2 cut(s) 1422, 1938
BseCI ATCGAT 1 cut(s) 1860
BseDI CCNNGG 4 cut(s) 459, 595, 872, 1420
BseLI CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2011, 2258
BseMI GCAATG 2 cut(s) 62, 546
BseMII CTCAG 4 cut(s) 1653, 1786, 2127, 2322
BseNI ACTGG 7 cut(s) 559, 710, 728, 1004, 1100, 1594, 2243
BseRI GAGGAG 3 cut(s) 20, 578, 1592
BseSI GKGCMC 2 cut(s) 1242, 1291
BseXI GCAGC 4 cut(s) 655, 1153, 1484, 1543
BseYI CCCAGC 1 cut(s) 298
Bsh1236I CGCG 1 cut(s) 350
Bsh1285I CGRYCG 1 cut(s) 2268
BshFI GGCC 9 cut(s) 160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
BshNI GGYRCC 1 cut(s) 1288
BshVI ATCGAT 1 cut(s) 1860
BsiEI CGRYCG 1 cut(s) 2268
BsiHKAI GWGCWC 3 cut(s) 337, 1242, 2026
BsiSI CCGG 2 cut(s) 1917, 2252
BslFI GGGAC 2 cut(s) 1423, 1735
BslI CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2011, 2258
BsmAI GTCTC 4 cut(s) 1075, 1276, 2074, 2132
BsmBI CGTCTC 1 cut(s) 1276
BsmFI GGGAC 2 cut(s) 1423, 1735
BsmI GAATGC 2 cut(s) 562, 564
BsnI GGCC 9 cut(s) 160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
Bso31I GGTCTC 2 cut(s) 1075, 2132
Bsp1286I GDGCHC 4 cut(s) 337, 1242, 1291, 2026
Bsp13I TCCGGA 1 cut(s) 2251
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 2 cut(s) 1339, 1734
BspANI GGCC 9 cut(s) 160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
BspCNI CTCAG 4 cut(s) 1654, 1785, 2128, 2321
BspDI ATCGAT 1 cut(s) 1860
BspEI TCCGGA 1 cut(s) 2251
BspFNI CGCG 1 cut(s) 350
BspHI TCATGA 2 cut(s) 717, 1846
BspLI GGNNCC 3 cut(s) 315, 1290, 2262
BspMAI CTGCAG 1 cut(s) 1561
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 3 cut(s) 308, 321, 645
BspQI GCTCTTC 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 1288
BspTI CTTAAG 1 cut(s) 1957
BspTNI GGTCTC 2 cut(s) 1075, 2132
BsrDI GCAATG 2 cut(s) 62, 546
BsrFI RCCGGY 1 cut(s) 1916
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 7 cut(s) 559, 710, 728, 1004, 1100, 1594, 2243
BssAI RCCGGY 1 cut(s) 1916
BssECI CCNNGG 4 cut(s) 459, 595, 872, 1420
BssMI GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
BssT1I CCWWGG 3 cut(s) 459, 595, 872
Bst2UI CCWGG 2 cut(s) 1422, 1938
Bst6I CTCTTC 5 cut(s) 380, 588, 877, 2028, 2188
BstAFI CTTAAG 1 cut(s) 1957
BstAPI GCANNNNNTGC 1 cut(s) 151
BstAUI TGTACA 1 cut(s) 123
BstC8I GCNNGC 6 cut(s) 539, 811, 1242, 1275, 1614, 1637
BstDEI CTNAG 6 cut(s) 155, 1662, 1772, 2067, 2136, 2308
BstDSI CCRYGG 1 cut(s) 459
BstFNI CGCG 1 cut(s) 350
BstKTI GATC 8 cut(s) 316, 653, 912, 1546, 1844, 1852, 2268, 2319
BstMAI GTCTC 4 cut(s) 1075, 1276, 2074, 2132
BstMBI GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
BstMCI CGRYCG 1 cut(s) 2268
BstMWI GCNNNNNNNGC 8 cut(s) 151, 253, 356, 794, 854, 1166, 1338, 1565
BstNI CCWGG 2 cut(s) 1422, 1938
BstNSI RCATGY 3 cut(s) 292, 1639, 2087
BstSCI CCNGG 2 cut(s) 1420, 1936
BstSFI CTRYAG 2 cut(s) 1035, 1557
BstSLI GKGCMC 2 cut(s) 1242, 1291
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 4 cut(s) 655, 1153, 1484, 1543
BstX2I RGATCY 3 cut(s) 313, 650, 1841
BstXI CCANNNNNNTGG 1 cut(s) 822
BstYI RGATCY 3 cut(s) 313, 650, 1841
Bsu15I ATCGAT 1 cut(s) 1860
BsuRI GGCC 9 cut(s) 160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
BsuTUI ATCGAT 1 cut(s) 1860
BtgI CCRYGG 1 cut(s) 459
BtsI GCAGTG 1 cut(s) 1551
BtsIMutI CAGTG 6 cut(s) 34, 838, 1071, 1203, 1551, 2338
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 6 cut(s) 539, 811, 1242, 1275, 1614, 1637
CaiI CAGNNNCTG 2 cut(s) 1421, 2288
CciI TCATGA 2 cut(s) 717, 1846
Cfr10I RCCGGY 1 cut(s) 1916
Cfr13I GGNCC 7 cut(s) 159, 463, 679, 1361, 1678, 1768, 2261
ClaI ATCGAT 1 cut(s) 1860
CseI GACGC 1 cut(s) 356
Csp6I GTAC 7 cut(s) 124, 259, 488, 829, 927, 1377, 2173
CspCI CAANNNNNGTGG 2 cut(s) 1353, 1388
CviQI GTAC 7 cut(s) 124, 259, 488, 829, 927, 1377, 2173
DdeI CTNAG 6 cut(s) 155, 1662, 1772, 2067, 2136, 2308
DpnI GATC 8 cut(s) 315, 652, 911, 1545, 1843, 1851, 2267, 2318
DpnII GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
DraIII CACNNNGTG 1 cut(s) 1712
DrdI GACNNNNNNGTC 2 cut(s) 1526, 2372
DseDI GACNNNNNNGTC 2 cut(s) 1526, 2372
EaeI YGGCCR 1 cut(s) 1735
Eam1104I CTCTTC 5 cut(s) 380, 588, 877, 2028, 2188
EarI CTCTTC 5 cut(s) 380, 588, 877, 2028, 2188
Eco130I CCWWGG 3 cut(s) 459, 595, 872
Eco31I GGTCTC 2 cut(s) 1075, 2132
Eco47I GGWCC 4 cut(s) 463, 679, 1768, 2261
Eco57I CTGAAG 2 cut(s) 641, 1295
EcoO109I RGGNCCY 2 cut(s) 159, 1768
EcoRI GAATTC 1 cut(s) 1797
EcoRII CCWGG 2 cut(s) 1420, 1936
EcoT14I CCWWGG 3 cut(s) 459, 595, 872
EcoT22I ATGCAT 2 cut(s) 564, 1655
ErhI CCWWGG 3 cut(s) 459, 595, 872
Esp3I CGTCTC 1 cut(s) 1276
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 2 cut(s) 1423, 1735
FauNDI CATATG 1 cut(s) 319
FbaI TGATCA 2 cut(s) 1849, 2316
FblI GTMKAC 3 cut(s) 1806, 1971, 1996
Fnu4HI GCNGC 5 cut(s) 669, 1167, 1473, 1557, 1735
Fsp4HI GCNGC 5 cut(s) 669, 1167, 1473, 1557, 1735
FspBI CTAG 8 cut(s) 203, 389, 471, 773, 1115, 1481, 2001, 2348
GluI GCNGC 5 cut(s) 669, 1167, 1473, 1557, 1735
GsaI CCCAGC 1 cut(s) 302
GsuI CTGGAG 1 cut(s) 1095
HaeIII GGCC 9 cut(s) 160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
HapII CCGG 2 cut(s) 1917, 2252
HgaI GACGC 1 cut(s) 356
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 2 cut(s) 969, 2045
HinfI GANTC 4 cut(s) 861, 1103, 1425, 1667
HpaII CCGG 2 cut(s) 1917, 2252
HphI GGTGA 4 cut(s) 699, 1942, 2218, 2282
Hpy166II GTNNAC 8 cut(s) 730, 767, 1240, 1501, 1759, 1807, 1972, 1997
Hpy188III TCNNGA 7 cut(s) 682, 718, 995, 1074, 1839, 1847, 2252
Hpy8I GTNNAC 8 cut(s) 730, 767, 1240, 1501, 1759, 1807, 1972, 1997
HpyAV CCTTC 7 cut(s) 107, 665, 740, 872, 1335, 1364, 1487
HpyF10VI GCNNNNNNNGC 8 cut(s) 151, 253, 356, 794, 854, 1166, 1338, 1565
HpyF3I CTNAG 6 cut(s) 155, 1662, 1772, 2067, 2136, 2308
Kpn2I TCCGGA 1 cut(s) 2251
Ksp22I TGATCA 2 cut(s) 1849, 2316
Kzo9I GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 3 cut(s) 1076, 1786, 1793
Lsp1109I GCAGC 4 cut(s) 655, 1153, 1484, 1543
LweI GCATC 3 cut(s) 47, 1975, 2128
MaeI CTAG 8 cut(s) 203, 389, 471, 773, 1115, 1481, 2001, 2348
MaeIII GTNAC 6 cut(s) 69, 1305, 1438, 1513, 1528, 1549
MalI GATC 8 cut(s) 315, 652, 911, 1545, 1843, 1851, 2267, 2318
MboI GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
MfeI CAATTG 1 cut(s) 2091
MflI RGATCY 3 cut(s) 313, 650, 1841
MhlI GDGCHC 4 cut(s) 337, 1242, 1291, 2026
MlyI GAGTC 1 cut(s) 1661
MmeI TCCRAC 2 cut(s) 85, 2347
Mph1103I ATGCAT 2 cut(s) 564, 1655
MroI TCCGGA 1 cut(s) 2251
MroXI GAANNNNTTC 1 cut(s) 562
MseI TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1958
MslI CAYNNNNRTG 3 cut(s) 293, 1208, 1938
MspCI CTTAAG 1 cut(s) 1957
MspI CCGG 2 cut(s) 1917, 2252
MspR9I CCNGG 2 cut(s) 1422, 1938
MunI CAATTG 1 cut(s) 2091
Mva1269I GAATGC 2 cut(s) 562, 564
MvaI CCWGG 2 cut(s) 1422, 1938
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 8 cut(s) 151, 253, 356, 794, 854, 1166, 1338, 1565
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 319
NdeII GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
NlaIV GGNNCC 3 cut(s) 315, 1290, 2262
NmuCI GTSAC 4 cut(s) 1305, 1513, 1528, 1549
NsiI ATGCAT 2 cut(s) 564, 1655
NspI RCATGY 3 cut(s) 292, 1639, 2087
PaeI GCATGC 1 cut(s) 1639
PagI TCATGA 2 cut(s) 717, 1846
PciI ACATGT 2 cut(s) 288, 2083
PciSI GCTCTTC 1 cut(s) 380
PctI GAATGC 2 cut(s) 562, 564
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 3 cut(s) 861, 1103, 1425
PkrI GCNGC 5 cut(s) 670, 1168, 1474, 1558, 1736
Ple19I CGATCG 1 cut(s) 2268
PleI GAGTC 1 cut(s) 1661
PpsI GAGTC 1 cut(s) 1661
PpuMI RGGWCCY 1 cut(s) 1768
PscI ACATGT 2 cut(s) 288, 2083
Psp5II RGGWCCY 1 cut(s) 1768
Psp6I CCWGG 2 cut(s) 1420, 1936
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 2 cut(s) 1420, 1936
PspN4I GGNNCC 3 cut(s) 315, 1290, 2262
PspPI GGNCC 7 cut(s) 159, 463, 679, 1361, 1678, 1768, 2261
PspPPI RGGWCCY 1 cut(s) 1768
PstI CTGCAG 1 cut(s) 1561
PstNI CAGNNNCTG 2 cut(s) 1421, 2288
PsuI RGATCY 3 cut(s) 313, 650, 1841
PvuI CGATCG 1 cut(s) 2268
RsaI GTAC 7 cut(s) 125, 260, 489, 830, 928, 1378, 2174
RsaNI GTAC 7 cut(s) 124, 259, 488, 829, 927, 1377, 2173
RseI CAYNNNNRTG 3 cut(s) 293, 1208, 1938
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1958
SatI GCNGC 5 cut(s) 669, 1167, 1473, 1557, 1735
Sau3AI GATC 8 cut(s) 313, 650, 909, 1543, 1841, 1849, 2265, 2316
Sau96I GGNCC 7 cut(s) 159, 463, 679, 1361, 1678, 1768, 2261
ScaI AGTACT 1 cut(s) 2174
SchI GAGTC 1 cut(s) 1661
ScrFI CCNGG 2 cut(s) 1422, 1938
SduI GDGCHC 4 cut(s) 337, 1242, 1291, 2026
SfaNI GCATC 3 cut(s) 47, 1975, 2128
SfcI CTRYAG 2 cut(s) 1035, 1557
SinI GGWCC 4 cut(s) 463, 679, 1768, 2261
SmiMI CAYNNNNRTG 3 cut(s) 293, 1208, 1938
SmlI CTYRAG 2 cut(s) 1957, 1990
SmoI CTYRAG 2 cut(s) 1957, 1990
SpeI ACTAGT 2 cut(s) 202, 1480
SphI GCATGC 1 cut(s) 1639
SsiI CCGC 2 cut(s) 1339, 1734
SspI AATATT 1 cut(s) 235
SspMI CTAG 8 cut(s) 203, 389, 471, 773, 1115, 1481, 2001, 2348
StyD4I CCNGG 2 cut(s) 1420, 1936
StyI CCWWGG 3 cut(s) 459, 595, 872
TaqI TCGA 3 cut(s) 1622, 1670, 1860
TatI WGTACW 4 cut(s) 123, 258, 487, 2172
TauI GCSGC 1 cut(s) 1737
TfiI GAWTC 3 cut(s) 861, 1103, 1425
Tru1I TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1958
Tru9I TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1958
TscAI CASTG 6 cut(s) 34, 838, 1071, 1210, 1558, 2338
TseFI GTSAC 4 cut(s) 1305, 1513, 1528, 1549
TseI GCWGC 4 cut(s) 668, 1166, 1472, 1556
Tsp45I GTSAC 4 cut(s) 1305, 1513, 1528, 1549
TspGWI ACGGA 1 cut(s) 1780
TspRI CASTG 6 cut(s) 34, 838, 1071, 1210, 1558, 2338
Vha464I CTTAAG 1 cut(s) 1957
VneI GTGCAC 1 cut(s) 1238
VpaK11BI GGWCC 4 cut(s) 463, 679, 1768, 2261
XapI RAATTY 4 cut(s) 21, 1369, 1797, 2291
XceI RCATGY 3 cut(s) 292, 1639, 2087
XcmI CCANNNNNNNNNTGG 2 cut(s) 1207, 2330
XmiI GTMKAC 3 cut(s) 1806, 1971, 1996
XmnI GAANNNNTTC 1 cut(s) 562
XspI CTAG 8 cut(s) 203, 389, 471, 773, 1115, 1481, 2001, 2348
ZrmI AGTACT 1 cut(s) 2174
Zsp2I ATGCAT 2 cut(s) 564, 1655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.