Rh1BG021200

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0014942
Physical Location & Seq
Reverse (-)
2712200 .. 2716582
4383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG021200.1

Sequence Viewer

Length: 2379 bp
ATGGAGGAGAGCAACAAAAAAATTCACAGTGTTGATAGAATTGACATTCCTAAAGGATGCTCATTGCAGTTACAAAAACTTTTCACAGATGTAGCCCTTCATGTTGGAGGTTTCTTCTTAATGTATATCCTGTGTCATCTTTCGCACTACCTGCTAAGGCCCTATTCACAGCCTCGCATTACCTCTGATACCTTTGTAGGACTAGTCATCGGAAACATAGGGTTCATACGCAATATTCTATCAAAGCGAGTATTGCAGTACATAGTTGATTTTGGAATGATTGGCTACATGTTTGTGCTGGGAATAGAAATGGATCCATATGTGCTTGTTAGAGCACCAACCAAAGACGCGAAAGTAGCCTATGCTGGTATGCTCTTCACATTCGCCCTGGCCTGTGGAATAACCCCATTTCTGAACTATACAGACGGTTTTAAGATTGACTTCACTCTCTCCCTCTCCATGGTCCTCTCTAGCACGGCTTCGCCTGTACTGACACGTATGATAACAAATCTCAAAATAGGCAAGTCTGACATAGGCCAGCTTGTCATTGCTACTGGAATGCATTCTGATTTTATAGCCACTCTTCTCCTCTCCCTTGGCTATGTCATTTGGCCTGTTCAAGTTGAAGATAAAAAGCCCAATGTTCGCAGATCCCTTCAGATGGGTGCTGCATTGTTGGTCCAGACAATTTTTACAGCCATTGTTTCACCAGTCGTCATGAACTGGGTCAACAATGAAAACCCCGAAGGCAAACCCATCAAAGGTTCACACCTAGTCCTCTCTATGGCTTTCATGGCCTTTGTTGTGGCTTGCCCATCATTTTGGGGGTACAGTGCTATTTTGAGTGCATTTATGGCAGGAATCTTTCTACCAAGGGAAGGAAGAGTATCAAAGTGGGTAATAGGCAAGATCAATTACTTGTTAAGTACCATATTCTATCCCATATTTTTCTTTTGGATGGGGTATGAAGCTTATTTTATGGAGTTTCAACCTCTTGACTGGATGACATGGGCAAGGTTATTTGTAGTTTTTGCTATAGCAACAATCGGAAAAGTTGCTGGAACAGTGGTCTCTGGAGCGTTGTTGGGGTTTAACTGGCCTGAATCTGTTGCACTTGGGCTTCTTCTCACTACCAAAGGCCATTTTCATATATACTTGGCTATTGCTGCAAAATCGTATGGGAAAACCACTACTTCAACCAGCACTGTGATGGTAATAGCTATTTTTTTTACTGTGGTGCACGCCCCATCAGTTGTGGCTCAAATTATTAAGCGTGCAAGGAGACGGGCACCCACTCATCGGATGTCACTTCAGTTGCTTGAACCTTCAAGCGAACTCCGCATATTGCTATGCCTTCATGGGCCACAAAATTTGCGTACAACCATTAACTTCATTGAGATATGTAGAGGGACAGCAGACCCTGGAATCGTGGTATTTGTAACAGACATGATAGAACTCACAGACCAAATAGCAGCCACACTAGTTAAAGAAGGAGTGAACACAATGACTGTGACTGACAAGGCAGTCACAGACAGGAGAGATCAAGTCACTGCTGCAGTTCAAGCCTATGTAGAGGAGGATGAAACTGGTATTACACTTGGACGATTGCTTGCACTTTCGACTTTCAATGGCATGCCCCAAGATATATGCATTTTGGCTGAGGACTCGATGGTGGCCCTCATCATATTGCCGTTTCACAAGACACAAGGTGCAGATGGGACATTAGATGGCGGCCATTCTGGTTTCAGATACGTGAACCGTAAGGTCCTCAGAAATGCTCCATGCTCCGTAGGAATTCTTGTAGACAGAGGTTTTGGACTGATTGAAAAAATATCAAGATCTCATGATCATTCTATCGATATGGCAGTCATCTTCATTGGTGGAAAAGATGATAGAGAAGCACTTGCTTATGCCGGTCATGTAGCACAACATCCTGGTGTAAAACTCACCATCTTAAGATTTTTGGTAGACAGTAGCTCGGATGCCTCAAGTAGACCTAGCCATTATCAGGTCAGTCGTGCTCTACAAGAAGAGGAAATGAAGCTTGATGATGAGTGCTTTGCTAAGTTCTACGAGAGACATGTTGCAATTGGGCAAGTTGCTTACATGGAGAAGCATCTTGCCAATTCTTCTGAGACCTATAATACCTTGATGACATTGGATGGGCAGTACTCACTTATTATAGTGGGAAGAGGGGAAAGGGTGAATACAGTTTTGACATTTGGGATGAATGACTGGCAGAAATGTCCGGAGTTGGGTCCGATCGGTGATGTTCTTTCAGGTTCTGAATTTTCAGTCAGAACCTCAGTTTTGATCATCCAACAAAACAGTGGAAAAGGAGAACTAGATGGACTTGATGATGACTTTTCTGTCATGTAA

Protein Analysis

792

Amino Acids

87.59

Weight (kDa)

6.71

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 44 - 423 5.1e-37 Sodium/hydrogen exchanger, transmembrane
CHX17_2nd PF23256 476 - 612 3e-14 Plant cation/H(+) antiporter domain
CHX17_C PF23259 618 - 779 1e-15 Plant cation/H(+) antiporter, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014942)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52080
fragaria_vesca FvH4_7g01210 FvH4_7g01210
malus_domestica MD02G1308800.v1.1 MD07G1013200.v1.1
prunus_persica Prupe.2G016700_v2.0.a1 Prupe.2G016700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0316681
rosa_laevigata RLG00000030644
rosa_multiflora Rmu_sc0005539.1_g000005
rosa_roxburghii Rroxscaffold_4G00330860
rosa_samantha Rh1AG022700 Rh1BG021200 Rh1CG019800 Rh1DG017200
rosa_wichuraiana Rw1G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1523, 2369
Acc36I ACCTGC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 1288
AccI GTMKAC 3 cut(s) 1803, 1968, 1993
AccII CGCG 1 cut(s) 350
AccIII TCCGGA 1 cut(s) 2248
AciI CCGC 2 cut(s) 1339, 1731
AclWI GGATC 3 cut(s) 308, 321, 645
AcoI YGGCCR 1 cut(s) 1732
AcsI RAATTY 4 cut(s) 21, 1369, 1794, 2288
AcuI CTGAAG 2 cut(s) 641, 1295
AdeI CACNNNGTG 1 cut(s) 1709
AfaI GTAC 6 cut(s) 260, 489, 830, 928, 1378, 2171
AfiI CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2008, 2255
AflII CTTAAG 1 cut(s) 1954
AflIII ACRYGT 3 cut(s) 288, 494, 2080
AgsI TTSAA 9 cut(s) 620, 626, 989, 1197, 1322, 1329, 1562, 1627, 1826
AhlI ACTAGT 2 cut(s) 202, 1480
AjnI CCWGG 3 cut(s) 387, 1420, 1933
AluBI AGCT 5 cut(s) 541, 971, 1220, 1977, 2044
AluI AGCT 5 cut(s) 541, 971, 1220, 1977, 2044
Alw21I GWGCWC 3 cut(s) 337, 1242, 2023
Alw26I GTCTC 4 cut(s) 1075, 1276, 2071, 2129
Alw44I GTGCAC 1 cut(s) 1238
AlwI GGATC 3 cut(s) 308, 321, 645
AlwNI CAGNNNCTG 2 cut(s) 1421, 2285
Aor13HI TCCGGA 1 cut(s) 2248
ApaLI GTGCAC 1 cut(s) 1238
ApeKI GCWGC 4 cut(s) 668, 1166, 1472, 1553
ApoI RAATTY 4 cut(s) 21, 1369, 1794, 2288
ArsI GACNNNNNNTTYG 2 cut(s) 1796, 1828
Asp700I GAANNNNTTC 1 cut(s) 562
AspS9I GGNCC 7 cut(s) 159, 463, 679, 1361, 1675, 1765, 2258
AsuHPI GGTGA 4 cut(s) 699, 1939, 2215, 2279
AvaII GGWCC 4 cut(s) 463, 679, 1765, 2258
BaeGI GKGCMC 2 cut(s) 1242, 1291
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 1288
Bbv12I GWGCWC 3 cut(s) 337, 1242, 2023
BbvCI CCTCAGC 1 cut(s) 1659
BbvI GCAGC 4 cut(s) 655, 1153, 1484, 1540
BceAI ACGGC 2 cut(s) 492, 1675
BciT130I CCWGG 3 cut(s) 389, 1422, 1935
BclI TGATCA 2 cut(s) 1846, 2313
BcoDI GTCTC 4 cut(s) 1075, 1276, 2071, 2129
BcuI ACTAGT 2 cut(s) 202, 1480
BfaI CTAG 6 cut(s) 203, 471, 773, 1481, 1998, 2345
BfmI CTRYAG 2 cut(s) 1035, 1554
BfrI CTTAAG 1 cut(s) 1954
BfuAI ACCTGC 1 cut(s) 159
BglII AGATCT 1 cut(s) 1838
BisI GCNGC 5 cut(s) 669, 1167, 1473, 1554, 1732
BlsI GCNGC 5 cut(s) 670, 1168, 1474, 1555, 1733
BmcAI AGTACT 1 cut(s) 2171
Bme1390I CCNGG 3 cut(s) 389, 1422, 1935
Bme18I GGWCC 4 cut(s) 463, 679, 1765, 2258
BmgT120I GGNCC 7 cut(s) 159, 463, 679, 1361, 1675, 1765, 2258
BmiI GGNNCC 3 cut(s) 315, 1290, 2259
BmrFI CCNGG 3 cut(s) 389, 1422, 1935
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 3 cut(s) 47, 1972, 2125
BmuI ACTGGG 1 cut(s) 733
BpmI CTGGAG 1 cut(s) 1095
Bpu10I CCTNAGC 2 cut(s) 155, 1659
BpuEI CTTGAG 1 cut(s) 1972
Bsa29I ATCGAT 1 cut(s) 1857
BsaAI YACGTR 2 cut(s) 497, 1753
BsaI GGTCTC 2 cut(s) 1075, 2129
BsaJI CCNNGG 5 cut(s) 387, 459, 595, 872, 1420
BsaWI WCCGGW 1 cut(s) 2248
Bsc4I CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2008, 2255
Bse118I RCCGGY 1 cut(s) 1913
Bse1I ACTGG 7 cut(s) 559, 710, 728, 1004, 1100, 1591, 2240
Bse3DI GCAATG 2 cut(s) 62, 546
BseAI TCCGGA 1 cut(s) 2248
BseBI CCWGG 3 cut(s) 389, 1422, 1935
BseCI ATCGAT 1 cut(s) 1857
BseDI CCNNGG 5 cut(s) 387, 459, 595, 872, 1420
BseLI CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2008, 2255
BseMI GCAATG 2 cut(s) 62, 546
BseMII CTCAG 4 cut(s) 1650, 1783, 2124, 2319
BseNI ACTGG 7 cut(s) 559, 710, 728, 1004, 1100, 1591, 2240
BseRI GAGGAG 3 cut(s) 20, 578, 1589
BseSI GKGCMC 2 cut(s) 1242, 1291
BseXI GCAGC 4 cut(s) 655, 1153, 1484, 1540
BseYI CCCAGC 1 cut(s) 298
BsgI GTGCAG 1 cut(s) 1731
Bsh1236I CGCG 1 cut(s) 350
Bsh1285I CGRYCG 1 cut(s) 2265
BshNI GGYRCC 1 cut(s) 1288
BshVI ATCGAT 1 cut(s) 1857
BsiEI CGRYCG 1 cut(s) 2265
BsiHKAI GWGCWC 3 cut(s) 337, 1242, 2023
BsiSI CCGG 2 cut(s) 1914, 2249
BslFI GGGAC 2 cut(s) 1423, 1732
BslI CCNNNNNNNGG 8 cut(s) 460, 661, 761, 784, 878, 1299, 2008, 2255
BsmAI GTCTC 4 cut(s) 1075, 1276, 2071, 2129
BsmBI CGTCTC 1 cut(s) 1276
BsmFI GGGAC 2 cut(s) 1423, 1732
BsmI GAATGC 2 cut(s) 562, 564
Bso31I GGTCTC 2 cut(s) 1075, 2129
Bsp1286I GDGCHC 4 cut(s) 337, 1242, 1291, 2023
Bsp13I TCCGGA 1 cut(s) 2248
Bsp143I GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 2 cut(s) 1339, 1731
BspCNI CTCAG 4 cut(s) 1651, 1782, 2125, 2318
BspDI ATCGAT 1 cut(s) 1857
BspEI TCCGGA 1 cut(s) 2248
BspFNI CGCG 1 cut(s) 350
BspHI TCATGA 2 cut(s) 717, 1843
BspLI GGNNCC 3 cut(s) 315, 1290, 2259
BspMAI CTGCAG 1 cut(s) 1558
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 3 cut(s) 308, 321, 645
BspQI GCTCTTC 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 1288
BspTI CTTAAG 1 cut(s) 1954
BspTNI GGTCTC 2 cut(s) 1075, 2129
BsrDI GCAATG 2 cut(s) 62, 546
BsrFI RCCGGY 1 cut(s) 1913
BsrI ACTGG 7 cut(s) 559, 710, 728, 1004, 1100, 1591, 2240
BssAI RCCGGY 1 cut(s) 1913
BssECI CCNNGG 5 cut(s) 387, 459, 595, 872, 1420
BssMI GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
BssT1I CCWWGG 3 cut(s) 459, 595, 872
Bst2UI CCWGG 3 cut(s) 389, 1422, 1935
Bst6I CTCTTC 5 cut(s) 380, 588, 877, 2025, 2185
BstAFI CTTAAG 1 cut(s) 1954
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 2 cut(s) 497, 1753
BstC8I GCNNGC 6 cut(s) 539, 811, 1242, 1275, 1611, 1634
BstDEI CTNAG 6 cut(s) 155, 1659, 1769, 2064, 2133, 2305
BstDSI CCRYGG 1 cut(s) 459
BstFNI CGCG 1 cut(s) 350
BstKTI GATC 8 cut(s) 316, 653, 912, 1543, 1841, 1849, 2265, 2316
BstMAI GTCTC 4 cut(s) 1075, 1276, 2071, 2129
BstMBI GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
BstMCI CGRYCG 1 cut(s) 2265
BstMWI GCNNNNNNNGC 8 cut(s) 151, 253, 356, 794, 854, 1166, 1338, 1562
BstNI CCWGG 3 cut(s) 389, 1422, 1935
BstNSI RCATGY 3 cut(s) 292, 1636, 2084
BstSCI CCNGG 3 cut(s) 387, 1420, 1933
BstSFI CTRYAG 2 cut(s) 1035, 1554
BstSLI GKGCMC 2 cut(s) 1242, 1291
BstUI CGCG 1 cut(s) 350
BstV1I GCAGC 4 cut(s) 655, 1153, 1484, 1540
BstX2I RGATCY 3 cut(s) 313, 650, 1838
BstXI CCANNNNNNTGG 1 cut(s) 822
BstYI RGATCY 3 cut(s) 313, 650, 1838
Bsu15I ATCGAT 1 cut(s) 1857
BsuTUI ATCGAT 1 cut(s) 1857
BtgI CCRYGG 1 cut(s) 459
BtsI GCAGTG 1 cut(s) 1548
BtsIMutI CAGTG 6 cut(s) 34, 838, 1071, 1203, 1548, 2335
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 6 cut(s) 539, 811, 1242, 1275, 1611, 1634
CaiI CAGNNNCTG 2 cut(s) 1421, 2285
CciI TCATGA 2 cut(s) 717, 1843
Cfr10I RCCGGY 1 cut(s) 1913
Cfr13I GGNCC 7 cut(s) 159, 463, 679, 1361, 1675, 1765, 2258
ClaI ATCGAT 1 cut(s) 1857
CseI GACGC 1 cut(s) 356
Csp6I GTAC 6 cut(s) 259, 488, 829, 927, 1377, 2170
CspCI CAANNNNNGTGG 2 cut(s) 1353, 1388
CviQI GTAC 6 cut(s) 259, 488, 829, 927, 1377, 2170
DdeI CTNAG 6 cut(s) 155, 1659, 1769, 2064, 2133, 2305
DpnI GATC 8 cut(s) 315, 652, 911, 1542, 1840, 1848, 2264, 2315
DpnII GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
DraIII CACNNNGTG 1 cut(s) 1709
DrdI GACNNNNNNGTC 2 cut(s) 1523, 2369
DseDI GACNNNNNNGTC 2 cut(s) 1523, 2369
EaeI YGGCCR 1 cut(s) 1732
Eam1104I CTCTTC 5 cut(s) 380, 588, 877, 2025, 2185
EarI CTCTTC 5 cut(s) 380, 588, 877, 2025, 2185
Eco130I CCWWGG 3 cut(s) 459, 595, 872
Eco31I GGTCTC 2 cut(s) 1075, 2129
Eco47I GGWCC 4 cut(s) 463, 679, 1765, 2258
Eco57I CTGAAG 2 cut(s) 641, 1295
EcoO109I RGGNCCY 2 cut(s) 159, 1765
EcoRI GAATTC 1 cut(s) 1794
EcoRII CCWGG 3 cut(s) 387, 1420, 1933
EcoT14I CCWWGG 3 cut(s) 459, 595, 872
EcoT22I ATGCAT 2 cut(s) 564, 1652
ErhI CCWWGG 3 cut(s) 459, 595, 872
Esp3I CGTCTC 1 cut(s) 1276
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 2 cut(s) 1423, 1732
FauNDI CATATG 1 cut(s) 319
FbaI TGATCA 2 cut(s) 1846, 2313
FblI GTMKAC 3 cut(s) 1803, 1968, 1993
Fnu4HI GCNGC 5 cut(s) 669, 1167, 1473, 1554, 1732
Fsp4HI GCNGC 5 cut(s) 669, 1167, 1473, 1554, 1732
FspBI CTAG 6 cut(s) 203, 471, 773, 1481, 1998, 2345
GluI GCNGC 5 cut(s) 669, 1167, 1473, 1554, 1732
GsaI CCCAGC 1 cut(s) 302
GsuI CTGGAG 1 cut(s) 1095
HapII CCGG 2 cut(s) 1914, 2249
HgaI GACGC 1 cut(s) 356
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 2 cut(s) 969, 2042
HinfI GANTC 4 cut(s) 861, 1103, 1425, 1664
HpaII CCGG 2 cut(s) 1914, 2249
HphI GGTGA 4 cut(s) 699, 1939, 2215, 2279
Hpy166II GTNNAC 8 cut(s) 730, 767, 1240, 1498, 1756, 1804, 1969, 1994
Hpy188III TCNNGA 7 cut(s) 682, 718, 995, 1074, 1836, 1844, 2249
Hpy8I GTNNAC 8 cut(s) 730, 767, 1240, 1498, 1756, 1804, 1969, 1994
HpyAV CCTTC 7 cut(s) 107, 665, 740, 872, 1335, 1364, 1484
HpyCH4IV ACGT 2 cut(s) 496, 1752
HpyF10VI GCNNNNNNNGC 8 cut(s) 151, 253, 356, 794, 854, 1166, 1338, 1562
HpyF3I CTNAG 6 cut(s) 155, 1659, 1769, 2064, 2133, 2305
HpySE526I ACGT 2 cut(s) 496, 1752
Kpn2I TCCGGA 1 cut(s) 2248
Ksp22I TGATCA 2 cut(s) 1846, 2313
Kzo9I GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 3 cut(s) 1076, 1783, 1790
Lsp1109I GCAGC 4 cut(s) 655, 1153, 1484, 1540
LweI GCATC 3 cut(s) 47, 1972, 2125
MaeI CTAG 6 cut(s) 203, 471, 773, 1481, 1998, 2345
MaeII ACGT 2 cut(s) 496, 1752
MaeIII GTNAC 6 cut(s) 69, 1305, 1438, 1510, 1525, 1546
MalI GATC 8 cut(s) 315, 652, 911, 1542, 1840, 1848, 2264, 2315
MboI GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
MfeI CAATTG 1 cut(s) 2088
MflI RGATCY 3 cut(s) 313, 650, 1838
MhlI GDGCHC 4 cut(s) 337, 1242, 1291, 2023
MlyI GAGTC 1 cut(s) 1658
MmeI TCCRAC 2 cut(s) 85, 2344
Mph1103I ATGCAT 2 cut(s) 564, 1652
MroI TCCGGA 1 cut(s) 2248
MroXI GAANNNNTTC 1 cut(s) 562
MseI TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1955
MslI CAYNNNNRTG 3 cut(s) 293, 1208, 1935
MspCI CTTAAG 1 cut(s) 1954
MspI CCGG 2 cut(s) 1914, 2249
MspR9I CCNGG 3 cut(s) 389, 1422, 1935
MunI CAATTG 1 cut(s) 2088
Mva1269I GAATGC 2 cut(s) 562, 564
MvaI CCWGG 3 cut(s) 389, 1422, 1935
MvnI CGCG 1 cut(s) 350
MwoI GCNNNNNNNGC 8 cut(s) 151, 253, 356, 794, 854, 1166, 1338, 1562
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 319
NdeII GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
NlaIV GGNNCC 3 cut(s) 315, 1290, 2259
NmuCI GTSAC 4 cut(s) 1305, 1510, 1525, 1546
NsiI ATGCAT 2 cut(s) 564, 1652
NspI RCATGY 3 cut(s) 292, 1636, 2084
PaeI GCATGC 1 cut(s) 1636
PagI TCATGA 2 cut(s) 717, 1843
PciI ACATGT 2 cut(s) 288, 2080
PciSI GCTCTTC 1 cut(s) 380
PctI GAATGC 2 cut(s) 562, 564
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 3 cut(s) 861, 1103, 1425
PkrI GCNGC 5 cut(s) 670, 1168, 1474, 1555, 1733
Ple19I CGATCG 1 cut(s) 2265
PleI GAGTC 1 cut(s) 1658
PpsI GAGTC 1 cut(s) 1658
Ppu21I YACGTR 2 cut(s) 497, 1753
PpuMI RGGWCCY 1 cut(s) 1765
PscI ACATGT 2 cut(s) 288, 2080
Psp5II RGGWCCY 1 cut(s) 1765
Psp6I CCWGG 3 cut(s) 387, 1420, 1933
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 3 cut(s) 387, 1420, 1933
PspN4I GGNNCC 3 cut(s) 315, 1290, 2259
PspPI GGNCC 7 cut(s) 159, 463, 679, 1361, 1675, 1765, 2258
PspPPI RGGWCCY 1 cut(s) 1765
PstI CTGCAG 1 cut(s) 1558
PstNI CAGNNNCTG 2 cut(s) 1421, 2285
PsuI RGATCY 3 cut(s) 313, 650, 1838
PvuI CGATCG 1 cut(s) 2265
RsaI GTAC 6 cut(s) 260, 489, 830, 928, 1378, 2171
RsaNI GTAC 6 cut(s) 259, 488, 829, 927, 1377, 2170
RseI CAYNNNNRTG 3 cut(s) 293, 1208, 1935
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1955
SatI GCNGC 5 cut(s) 669, 1167, 1473, 1554, 1732
Sau3AI GATC 8 cut(s) 313, 650, 909, 1540, 1838, 1846, 2262, 2313
Sau96I GGNCC 7 cut(s) 159, 463, 679, 1361, 1675, 1765, 2258
ScaI AGTACT 1 cut(s) 2171
SchI GAGTC 1 cut(s) 1658
ScrFI CCNGG 3 cut(s) 389, 1422, 1935
SduI GDGCHC 4 cut(s) 337, 1242, 1291, 2023
SfaNI GCATC 3 cut(s) 47, 1972, 2125
SfcI CTRYAG 2 cut(s) 1035, 1554
SinI GGWCC 4 cut(s) 463, 679, 1765, 2258
SmiMI CAYNNNNRTG 3 cut(s) 293, 1208, 1935
SmlI CTYRAG 2 cut(s) 1954, 1987
SmoI CTYRAG 2 cut(s) 1954, 1987
SpeI ACTAGT 2 cut(s) 202, 1480
SphI GCATGC 1 cut(s) 1636
SsiI CCGC 2 cut(s) 1339, 1731
SspI AATATT 1 cut(s) 235
SspMI CTAG 6 cut(s) 203, 471, 773, 1481, 1998, 2345
StyD4I CCNGG 3 cut(s) 387, 1420, 1933
StyI CCWWGG 3 cut(s) 459, 595, 872
TaiI ACGT 2 cut(s) 499, 1755
TaqI TCGA 3 cut(s) 1619, 1667, 1857
TatI WGTACW 3 cut(s) 258, 487, 2169
TauI GCSGC 1 cut(s) 1734
TfiI GAWTC 3 cut(s) 861, 1103, 1425
Tru1I TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1955
Tru9I TTAA 8 cut(s) 119, 432, 923, 1092, 1269, 1386, 1485, 1955
TscAI CASTG 6 cut(s) 34, 838, 1071, 1210, 1555, 2335
TseFI GTSAC 4 cut(s) 1305, 1510, 1525, 1546
TseI GCWGC 4 cut(s) 668, 1166, 1472, 1553
Tsp45I GTSAC 4 cut(s) 1305, 1510, 1525, 1546
TspGWI ACGGA 1 cut(s) 1777
TspRI CASTG 6 cut(s) 34, 838, 1071, 1210, 1555, 2335
Vha464I CTTAAG 1 cut(s) 1954
VneI GTGCAC 1 cut(s) 1238
VpaK11BI GGWCC 4 cut(s) 463, 679, 1765, 2258
XapI RAATTY 4 cut(s) 21, 1369, 1794, 2288
XceI RCATGY 3 cut(s) 292, 1636, 2084
XcmI CCANNNNNNNNNTGG 2 cut(s) 1207, 2327
XmiI GTMKAC 3 cut(s) 1803, 1968, 1993
XmnI GAANNNNTTC 1 cut(s) 562
XspI CTAG 6 cut(s) 203, 471, 773, 1481, 1998, 2345
ZrmI AGTACT 1 cut(s) 2171
Zsp2I ATGCAT 2 cut(s) 564, 1652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.