Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0014942

Physical Location & Seq

Genomic Coordinates Forward (+)

64634553 .. 64638983

Gene Structure 4431 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00330860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGGAGGAGAGCAACAAAAAAATTCACAGTGTTGTTAGAATCGACATTCCTAAAGGATGCTCATTACAGTTGCAAAAACTTTTCACAGATGTAGCCCTTCATGTTGGAGGTTTCTTCTTAATGTATATCCTGTGTCATCTTTCGCACTACCTGCTAAGGCCCTATTCACAGCCTCGCATTACCTCTGATACCTTTGTAGGACTAGTCATCGGAAACATAGGGTTCATACGCGATATTCTATCAAAGCGAGTATTGCAGTACATAGTTGATTTTGGAATGATTGGCTACATGTTTGTGCTGGGAATAGAAATGGATCCATATGTGCTTGTTAGAGCACCAACCAAAGACGCCAAAGTAGCCTATGCTGGTATGCTCTTCACATTCGCCCTAGCCTGTGGAATAACCCCATTTCTGAACTATACAGACGGTTTTAAGATTGACTTCACTCTCTCCCTCTCCATGGTCCTCTCTAGCACGGCTTCGCCTGTACTGACACGTATGATAACAAATCTCAAAATAGGCAAGTCTGACATAGGCCAGCTTGTCATTGCTACTGGAATGCATTCTGATTTTATAGCCACTCTTCTCCTCTCCCTTGGCTATGTCATTTGGCCTGTTCAAGTTGAAGATAAAAAGCCCAATGTTCGCAGATCCCTTCAGATGGGTGCTGCATTGTTGGTCCAGACAATTTTTACAGCCATTGTTTCACCAGTCGTCATGAACTGGGTCAACAATGAAAACCCCGAAGGCAAACCCATCAAAGGTTCACACCTAGTCCTCTCTATGGCTTTCATGGCCTTTGTTGTGGCTTGCCCATCATTTTGGGGGTACAGTGCTATTTTGAGTGCATTTATGGCAGGAATCTTTCTACCAAGGGAAGGAAGAGTATCAAAGTGGGTAATAGGCAAGATCAATTACTTGTTAAGTACCATATTCTATCCCATATTTTTCTTTTGGATGGGGTATGAAGCTTATTTTATGGAGTTTCAACCTCTTGACTGGATGACATGGGCAAGGTTATTTGTAGTTTTTGCTATAGCAACAATTGGAAAAGTTGCTGGAACAGTGGTCTCTGGAGCGTTGTTGGGGTTTAACTGGCCTGAGTCTGTTGCACTTGGGCTTCTTCTCACTACCAAAGGCCATTTTCATATATACTTGGCTATTGCTGCAAAATCGTATGGGAAAACCACTACTTCAACCAGCACTGTGATGGTAATAGCTATTTTTTTTACTGTGGTGCACGCCCCATCAGTTGTGGCTCAAATTATTAAGCGTGCAAGGAGACGGGCGCCCACTCATCGGATGTCACTTCAGTTGCTTGAACCTTCAAGCGAACTCCGCATATTGCTATGCCTTCATGGGCCACAAAATTTGCGTACAACCATTAACTTCATTGAGATATGTAGAGGGACAGCGGACCCTGGAATCGTGGTATTTGTAACAGACATGATAGAACTCACAGACCAAATAGCAGCCACACTAGTTAAAGAAGAAGGAGTGAACACAATGACTGTGACTGACAAGGCAGTCACAGACAGGAGAGATCAAGTCACTGCTGCAGTTCAAGCCTATGTAGAGGAGGATGAAACTGGTATTACACTTGGACGATTGCTTGCACTTTCAACTTTCAATGGCATGCCCCAAGATATATGCATTTTGGCTGAGGACTCGATGGTGGCCCTCATCATATTGCCGTTTCACAAGACACAAGGTGCAGATGGGACATTAGATGGCGGCCATTCTGGTTTCAGATATGTGAACCGTAAGGTCCTCAGAAATGCTCCATGCTCCGTAGGAATTCTTGTAGACAGAGGTTTTGGACTGATTGAAAAAATATCAAGATCTCATGATCATTCTATCGATATGGCAGTCATCTTCATTGGTGGAAAAGATGATAGAGAAGCACTTGCTTATGCCGGTCATGTAGCACAACATCCTGGTGTAAAACTCACCATCTTAAGATTTTTGGTAGACAGTAGCTCGGATGCCTCAAGTAGACCTAGCCATTATCAGGTCAGTCGTGCTCTACAAGAAGAGGAAATGAAGCTTGATGATGAGTGCTTTGCTAAGTTCTACGAGAGACATGTTGCAATTGGGCAAGTTGCTTACATGGAGAAGCATCTTGCCAATTCTTCTGAGACCTATAATACCTTGATGACATTGGATGGGCAGTACTCACTTATTATAGTGGGAAGAGGGGAAAGGGTGAATACAGTTTTGACATTTGGGATGAATGACTGGCAGAAATGTCCGGAGTTGGGTCCGATCGGTGATGTTCTTTCAGGTTCTGAATTTTCAGTCAGAACCTCAGTTTTGATCATCCAACAAAACAGTGGAAAAGGAGAACTAGATGGACTTGATGATGACTTTTCTGTCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0016020 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

793

Amino Acids

87.71

Weight (kDa)

6.58

Isoelectric Point (pI)

38.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	44 - 423	4.7e-37	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	476 - 613	7.5e-15	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	619 - 780	1e-15	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014942)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52080
fragaria_vesca	FvH4_7g01210 FvH4_7g01210
malus_domestica	MD02G1308800.v1.1 MD07G1013200.v1.1
prunus_persica	Prupe.2G016700_v2.0.a1 Prupe.2G016700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0316681
rosa_laevigata	RLG00000030644
rosa_multiflora	Rmu_sc0005539.1_g000005
rosa_roxburghii	Rroxscaffold_4G00330860
rosa_samantha	Rh1AG022700 Rh1BG021200 Rh1CG019800 Rh1DG017200
rosa_wichuraiana	Rw1G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1526, 2372
Acc36I	ACCTGC	1 cut(s)	159
AccB1I	GGYRCC	1 cut(s)	1288
AccI	GTMKAC	3 cut(s)	1806, 1971, 1996
AccII	CGCG	1 cut(s)	231
AccIII	TCCGGA	1 cut(s)	2251
AciI	CCGC	3 cut(s)	1339, 1415, 1734
AclWI	GGATC	3 cut(s)	308, 321, 645
AcoI	YGGCCR	1 cut(s)	1735
AcsI	RAATTY	4 cut(s)	21, 1369, 1797, 2291
AcuI	CTGAAG	2 cut(s)	641, 1295
AcyI	GRCGYC	2 cut(s)	348, 1289
AdeI	CACNNNGTG	1 cut(s)	1712
AfaI	GTAC	6 cut(s)	260, 489, 830, 928, 1378, 2174
AfiI	CCNNNNNNNGG	8 cut(s)	460, 661, 761, 784, 878, 1299, 2011, 2258
AflII	CTTAAG	1 cut(s)	1957
AflIII	ACRYGT	3 cut(s)	288, 494, 2083
AhlI	ACTAGT	2 cut(s)	202, 1480
AjnI	CCWGG	2 cut(s)	1420, 1936
AluBI	AGCT	5 cut(s)	541, 971, 1220, 1980, 2047
AluI	AGCT	5 cut(s)	541, 971, 1220, 1980, 2047
Alw21I	GWGCWC	3 cut(s)	337, 1242, 2026
Alw26I	GTCTC	4 cut(s)	1075, 1276, 2074, 2132
Alw44I	GTGCAC	1 cut(s)	1238
AlwI	GGATC	3 cut(s)	308, 321, 645
AlwNI	CAGNNNCTG	1 cut(s)	2288
Aor13HI	TCCGGA	1 cut(s)	2251
AoxI	GGCC	9 cut(s)	158, 535, 611, 795, 1097, 1138, 1361, 1677, 1735
ApaLI	GTGCAC	1 cut(s)	1238
ApeKI	GCWGC	4 cut(s)	668, 1166, 1472, 1556
ApoI	RAATTY	4 cut(s)	21, 1369, 1797, 2291
ArsI	GACNNNNNNTTYG	2 cut(s)	1799, 1831
Asp700I	GAANNNNTTC	1 cut(s)	562
AspLEI	GCGC	1 cut(s)	1291
AspS9I	GGNCC	8 cut(s)	159, 463, 679, 1361, 1417, 1678, 1768, 2261
AsuHPI	GGTGA	4 cut(s)	699, 1942, 2218, 2282
AvaII	GGWCC	5 cut(s)	463, 679, 1417, 1768, 2261
BaeGI	GKGCMC	1 cut(s)	1242
BaeI	ACNNNNGTAYC	2 cut(s)	180, 213
BamHI	GGATCC	1 cut(s)	313
BanI	GGYRCC	1 cut(s)	1288
Bbv12I	GWGCWC	3 cut(s)	337, 1242, 2026
BbvCI	CCTCAGC	1 cut(s)	1662
BbvI	GCAGC	4 cut(s)	655, 1153, 1484, 1543
BceAI	ACGGC	2 cut(s)	492, 1678
BciT130I	CCWGG	2 cut(s)	1422, 1938
BclI	TGATCA	2 cut(s)	1849, 2316
BcoDI	GTCTC	4 cut(s)	1075, 1276, 2074, 2132
BcuI	ACTAGT	2 cut(s)	202, 1480
BfaI	CTAG	7 cut(s)	203, 389, 471, 773, 1481, 2001, 2348
BfmI	CTRYAG	2 cut(s)	1035, 1557
BfoI	RGCGCY	1 cut(s)	1292
BfrI	CTTAAG	1 cut(s)	1957
BfuAI	ACCTGC	1 cut(s)	159
BglII	AGATCT	1 cut(s)	1841
BisI	GCNGC	5 cut(s)	669, 1167, 1473, 1557, 1735
BlsI	GCNGC	5 cut(s)	670, 1168, 1474, 1558, 1736
BmcAI	AGTACT	1 cut(s)	2174
Bme1390I	CCNGG	2 cut(s)	1422, 1938
Bme18I	GGWCC	5 cut(s)	463, 679, 1417, 1768, 2261
BmgT120I	GGNCC	8 cut(s)	159, 463, 679, 1361, 1417, 1678, 1768, 2261
BmiI	GGNNCC	4 cut(s)	315, 1290, 1419, 2262
BmrFI	CCNGG	2 cut(s)	1422, 1938
BmrI	ACTGGG	1 cut(s)	733
BmsI	GCATC	3 cut(s)	47, 1975, 2128
BmuI	ACTGGG	1 cut(s)	733
BpmI	CTGGAG	1 cut(s)	1095
Bpu10I	CCTNAGC	2 cut(s)	155, 1662
BpuEI	CTTGAG	1 cut(s)	1975
Bsa29I	ATCGAT	1 cut(s)	1860
BsaAI	YACGTR	1 cut(s)	497
BsaHI	GRCGYC	2 cut(s)	348, 1289
BsaI	GGTCTC	2 cut(s)	1075, 2132
BsaJI	CCNNGG	4 cut(s)	459, 595, 872, 1420
BsaWI	WCCGGW	1 cut(s)	2251
Bsc4I	CCNNNNNNNGG	8 cut(s)	460, 661, 761, 784, 878, 1299, 2011, 2258
Bse118I	RCCGGY	1 cut(s)	1916
Bse1I	ACTGG	7 cut(s)	559, 710, 728, 1004, 1100, 1594, 2243
Bse3DI	GCAATG	1 cut(s)	546
BseAI	TCCGGA	1 cut(s)	2251
BseBI	CCWGG	2 cut(s)	1422, 1938
BseCI	ATCGAT	1 cut(s)	1860
BseDI	CCNNGG	4 cut(s)	459, 595, 872, 1420
BseLI	CCNNNNNNNGG	8 cut(s)	460, 661, 761, 784, 878, 1299, 2011, 2258
BseMI	GCAATG	1 cut(s)	546
BseMII	CTCAG	5 cut(s)	1092, 1653, 1786, 2127, 2322
BseNI	ACTGG	7 cut(s)	559, 710, 728, 1004, 1100, 1594, 2243
BseRI	GAGGAG	3 cut(s)	20, 578, 1592
BseSI	GKGCMC	1 cut(s)	1242
BseXI	GCAGC	4 cut(s)	655, 1153, 1484, 1543
BseYI	CCCAGC	1 cut(s)	298
BsgI	GTGCAG	1 cut(s)	1734
Bsh1236I	CGCG	1 cut(s)	231
Bsh1285I	CGRYCG	1 cut(s)	2268
BshFI	GGCC	9 cut(s)	160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
BshNI	GGYRCC	1 cut(s)	1288
BshVI	ATCGAT	1 cut(s)	1860
BsiEI	CGRYCG	1 cut(s)	2268
BsiHKAI	GWGCWC	3 cut(s)	337, 1242, 2026
BsiSI	CCGG	2 cut(s)	1917, 2252
BslFI	GGGAC	2 cut(s)	1423, 1735
BslI	CCNNNNNNNGG	8 cut(s)	460, 661, 761, 784, 878, 1299, 2011, 2258
BsmAI	GTCTC	4 cut(s)	1075, 1276, 2074, 2132
BsmBI	CGTCTC	1 cut(s)	1276
BsmFI	GGGAC	2 cut(s)	1423, 1735
BsmI	GAATGC	2 cut(s)	562, 564
BsnI	GGCC	9 cut(s)	160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
Bso31I	GGTCTC	2 cut(s)	1075, 2132
Bsp1286I	GDGCHC	3 cut(s)	337, 1242, 2026
Bsp13I	TCCGGA	1 cut(s)	2251
Bsp143I	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
Bsp19I	CCATGG	1 cut(s)	459
BspACI	CCGC	3 cut(s)	1339, 1415, 1734
BspANI	GGCC	9 cut(s)	160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
BspCNI	CTCAG	5 cut(s)	1093, 1654, 1785, 2128, 2321
BspDI	ATCGAT	1 cut(s)	1860
BspEI	TCCGGA	1 cut(s)	2251
BspFNI	CGCG	1 cut(s)	231
BspHI	TCATGA	2 cut(s)	717, 1846
BspLI	GGNNCC	4 cut(s)	315, 1290, 1419, 2262
BspMAI	CTGCAG	1 cut(s)	1561
BspMI	ACCTGC	1 cut(s)	159
BspPI	GGATC	3 cut(s)	308, 321, 645
BspQI	GCTCTTC	1 cut(s)	380
BspT107I	GGYRCC	1 cut(s)	1288
BspTI	CTTAAG	1 cut(s)	1957
BspTNI	GGTCTC	2 cut(s)	1075, 2132
BsrDI	GCAATG	1 cut(s)	546
BsrFI	RCCGGY	1 cut(s)	1916
BsrI	ACTGG	7 cut(s)	559, 710, 728, 1004, 1100, 1594, 2243
BssAI	RCCGGY	1 cut(s)	1916
BssECI	CCNNGG	4 cut(s)	459, 595, 872, 1420
BssMI	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
BssNI	GRCGYC	2 cut(s)	348, 1289
BssT1I	CCWWGG	3 cut(s)	459, 595, 872
Bst2UI	CCWGG	2 cut(s)	1422, 1938
Bst6I	CTCTTC	5 cut(s)	380, 588, 877, 2028, 2188
BstACI	GRCGYC	2 cut(s)	348, 1289
BstAFI	CTTAAG	1 cut(s)	1957
BstAPI	GCANNNNNTGC	1 cut(s)	151
BstBAI	YACGTR	1 cut(s)	497
BstC8I	GCNNGC	6 cut(s)	539, 811, 1242, 1275, 1614, 1637
BstDEI	CTNAG	7 cut(s)	155, 1101, 1662, 1772, 2067, 2136, 2308
BstDSI	CCRYGG	1 cut(s)	459
BstFNI	CGCG	1 cut(s)	231
BstH2I	RGCGCY	1 cut(s)	1292
BstHHI	GCGC	1 cut(s)	1291
BstKTI	GATC	8 cut(s)	316, 653, 912, 1546, 1844, 1852, 2268, 2319
BstMAI	GTCTC	4 cut(s)	1075, 1276, 2074, 2132
BstMBI	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
BstMCI	CGRYCG	1 cut(s)	2268
BstMWI	GCNNNNNNNGC	8 cut(s)	151, 253, 356, 794, 854, 1166, 1338, 1565
BstNI	CCWGG	2 cut(s)	1422, 1938
BstNSI	RCATGY	3 cut(s)	292, 1639, 2087
BstSCI	CCNGG	2 cut(s)	1420, 1936
BstSFI	CTRYAG	2 cut(s)	1035, 1557
BstSLI	GKGCMC	1 cut(s)	1242
BstUI	CGCG	1 cut(s)	231
BstV1I	GCAGC	4 cut(s)	655, 1153, 1484, 1543
BstX2I	RGATCY	3 cut(s)	313, 650, 1841
BstXI	CCANNNNNNTGG	1 cut(s)	822
BstYI	RGATCY	3 cut(s)	313, 650, 1841
Bsu15I	ATCGAT	1 cut(s)	1860
BsuRI	GGCC	9 cut(s)	160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
BsuTUI	ATCGAT	1 cut(s)	1860
BtgI	CCRYGG	1 cut(s)	459
BtsI	GCAGTG	1 cut(s)	1551
BtsIMutI	CAGTG	6 cut(s)	34, 838, 1071, 1203, 1551, 2338
BveI	ACCTGC	1 cut(s)	159
Cac8I	GCNNGC	6 cut(s)	539, 811, 1242, 1275, 1614, 1637
CaiI	CAGNNNCTG	1 cut(s)	2288
CciI	TCATGA	2 cut(s)	717, 1846
CfoI	GCGC	1 cut(s)	1291
Cfr10I	RCCGGY	1 cut(s)	1916
Cfr13I	GGNCC	8 cut(s)	159, 463, 679, 1361, 1417, 1678, 1768, 2261
ClaI	ATCGAT	1 cut(s)	1860
CseI	GACGC	1 cut(s)	356
Csp6I	GTAC	6 cut(s)	259, 488, 829, 927, 1377, 2173
CspCI	CAANNNNNGTGG	2 cut(s)	1353, 1388
CviQI	GTAC	6 cut(s)	259, 488, 829, 927, 1377, 2173
DdeI	CTNAG	7 cut(s)	155, 1101, 1662, 1772, 2067, 2136, 2308
DinI	GGCGCC	1 cut(s)	1290
DpnI	GATC	8 cut(s)	315, 652, 911, 1545, 1843, 1851, 2267, 2318
DpnII	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
DraIII	CACNNNGTG	1 cut(s)	1712
DrdI	GACNNNNNNGTC	2 cut(s)	1526, 2372
DseDI	GACNNNNNNGTC	2 cut(s)	1526, 2372
EaeI	YGGCCR	1 cut(s)	1735
Eam1104I	CTCTTC	5 cut(s)	380, 588, 877, 2028, 2188
EarI	CTCTTC	5 cut(s)	380, 588, 877, 2028, 2188
Eco130I	CCWWGG	3 cut(s)	459, 595, 872
Eco31I	GGTCTC	2 cut(s)	1075, 2132
Eco47I	GGWCC	5 cut(s)	463, 679, 1417, 1768, 2261
Eco57I	CTGAAG	2 cut(s)	641, 1295
EcoO109I	RGGNCCY	2 cut(s)	159, 1768
EcoRI	GAATTC	1 cut(s)	1797
EcoRII	CCWGG	2 cut(s)	1420, 1936
EcoT14I	CCWWGG	3 cut(s)	459, 595, 872
EcoT22I	ATGCAT	2 cut(s)	564, 1655
EgeI	GGCGCC	1 cut(s)	1290
EheI	GGCGCC	1 cut(s)	1290
ErhI	CCWWGG	3 cut(s)	459, 595, 872
Esp3I	CGTCTC	1 cut(s)	1276
FalI	AAGNNNNNCTT	2 cut(s)	425, 457
FaqI	GGGAC	2 cut(s)	1423, 1735
FauNDI	CATATG	1 cut(s)	319
FbaI	TGATCA	2 cut(s)	1849, 2316
FblI	GTMKAC	3 cut(s)	1806, 1971, 1996
Fnu4HI	GCNGC	5 cut(s)	669, 1167, 1473, 1557, 1735
Fsp4HI	GCNGC	5 cut(s)	669, 1167, 1473, 1557, 1735
FspBI	CTAG	7 cut(s)	203, 389, 471, 773, 1481, 2001, 2348
GlaI	GCGC	1 cut(s)	1290
GluI	GCNGC	5 cut(s)	669, 1167, 1473, 1557, 1735
GsaI	CCCAGC	1 cut(s)	302
GsuI	CTGGAG	1 cut(s)	1095
HaeII	RGCGCY	1 cut(s)	1292
HaeIII	GGCC	9 cut(s)	160, 537, 613, 797, 1099, 1140, 1363, 1679, 1737
HapII	CCGG	2 cut(s)	1917, 2252
HgaI	GACGC	1 cut(s)	356
HhaI	GCGC	1 cut(s)	1291
Hin1I	GRCGYC	2 cut(s)	348, 1289
Hin6I	GCGC	1 cut(s)	1289
HinP1I	GCGC	1 cut(s)	1289
HincII	GTYRAC	1 cut(s)	730
HindII	GTYRAC	1 cut(s)	730
HindIII	AAGCTT	2 cut(s)	969, 2045
HinfI	GANTC	5 cut(s)	39, 861, 1103, 1425, 1667
HpaII	CCGG	2 cut(s)	1917, 2252
HphI	GGTGA	4 cut(s)	699, 1942, 2218, 2282
Hpy166II	GTNNAC	8 cut(s)	730, 767, 1240, 1501, 1759, 1807, 1972, 1997
Hpy188III	TCNNGA	7 cut(s)	682, 718, 995, 1074, 1839, 1847, 2252
Hpy8I	GTNNAC	8 cut(s)	730, 767, 1240, 1501, 1759, 1807, 1972, 1997
HpyAV	CCTTC	7 cut(s)	107, 665, 740, 872, 1335, 1364, 1487
HpyCH4IV	ACGT	1 cut(s)	496
HpyF10VI	GCNNNNNNNGC	8 cut(s)	151, 253, 356, 794, 854, 1166, 1338, 1565
HpyF3I	CTNAG	7 cut(s)	155, 1101, 1662, 1772, 2067, 2136, 2308
HpySE526I	ACGT	1 cut(s)	496
Hsp92I	GRCGYC	2 cut(s)	348, 1289
HspAI	GCGC	1 cut(s)	1289
KasI	GGCGCC	1 cut(s)	1288
Kpn2I	TCCGGA	1 cut(s)	2251
Ksp22I	TGATCA	2 cut(s)	1849, 2316
Kzo9I	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
LguI	GCTCTTC	1 cut(s)	380
LmnI	GCTCC	3 cut(s)	1076, 1786, 1793
Lsp1109I	GCAGC	4 cut(s)	655, 1153, 1484, 1543
LweI	GCATC	3 cut(s)	47, 1975, 2128
MaeI	CTAG	7 cut(s)	203, 389, 471, 773, 1481, 2001, 2348
MaeII	ACGT	1 cut(s)	496
MaeIII	GTNAC	5 cut(s)	1305, 1438, 1513, 1528, 1549
MalI	GATC	8 cut(s)	315, 652, 911, 1545, 1843, 1851, 2267, 2318
MboI	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
MfeI	CAATTG	2 cut(s)	1044, 2091
MflI	RGATCY	3 cut(s)	313, 650, 1841
MhlI	GDGCHC	3 cut(s)	337, 1242, 2026
Mly113I	GGCGCC	1 cut(s)	1289
MlyI	GAGTC	2 cut(s)	1112, 1661
MmeI	TCCRAC	2 cut(s)	85, 2347
Mph1103I	ATGCAT	2 cut(s)	564, 1655
MroI	TCCGGA	1 cut(s)	2251
MroXI	GAANNNNTTC	1 cut(s)	562
MseI	TTAA	8 cut(s)	119, 432, 923, 1092, 1269, 1386, 1485, 1958
MslI	CAYNNNNRTG	3 cut(s)	293, 1208, 1938
MspA1I	CMGCKG	1 cut(s)	1415
MspCI	CTTAAG	1 cut(s)	1957
MspI	CCGG	2 cut(s)	1917, 2252
MspR9I	CCNGG	2 cut(s)	1422, 1938
MunI	CAATTG	2 cut(s)	1044, 2091
Mva1269I	GAATGC	2 cut(s)	562, 564
MvaI	CCWGG	2 cut(s)	1422, 1938
MvnI	CGCG	1 cut(s)	231
MwoI	GCNNNNNNNGC	8 cut(s)	151, 253, 356, 794, 854, 1166, 1338, 1565
NarI	GGCGCC	1 cut(s)	1289
NcoI	CCATGG	1 cut(s)	459
NdeI	CATATG	1 cut(s)	319
NdeII	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
NlaIV	GGNNCC	4 cut(s)	315, 1290, 1419, 2262
NmuCI	GTSAC	4 cut(s)	1305, 1513, 1528, 1549
NsiI	ATGCAT	2 cut(s)	564, 1655
NspI	RCATGY	3 cut(s)	292, 1639, 2087
PaeI	GCATGC	1 cut(s)	1639
PagI	TCATGA	2 cut(s)	717, 1846
PciI	ACATGT	2 cut(s)	288, 2083
PciSI	GCTCTTC	1 cut(s)	380
PctI	GAATGC	2 cut(s)	562, 564
PdmI	GAANNNNTTC	1 cut(s)	562
PfeI	GAWTC	3 cut(s)	39, 861, 1425
PkrI	GCNGC	5 cut(s)	670, 1168, 1474, 1558, 1736
Ple19I	CGATCG	1 cut(s)	2268
PleI	GAGTC	2 cut(s)	1111, 1661
PluTI	GGCGCC	1 cut(s)	1292
PpsI	GAGTC	2 cut(s)	1111, 1661
Ppu21I	YACGTR	1 cut(s)	497
PpuMI	RGGWCCY	1 cut(s)	1768
PscI	ACATGT	2 cut(s)	288, 2083
Psp5II	RGGWCCY	1 cut(s)	1768
Psp6I	CCWGG	2 cut(s)	1420, 1936
PspFI	CCCAGC	1 cut(s)	298
PspGI	CCWGG	2 cut(s)	1420, 1936
PspN4I	GGNNCC	4 cut(s)	315, 1290, 1419, 2262
PspPI	GGNCC	8 cut(s)	159, 463, 679, 1361, 1417, 1678, 1768, 2261
PspPPI	RGGWCCY	1 cut(s)	1768
PstI	CTGCAG	1 cut(s)	1561
PstNI	CAGNNNCTG	1 cut(s)	2288
PsuI	RGATCY	3 cut(s)	313, 650, 1841
PvuI	CGATCG	1 cut(s)	2268
RsaI	GTAC	6 cut(s)	260, 489, 830, 928, 1378, 2174
RsaNI	GTAC	6 cut(s)	259, 488, 829, 927, 1377, 2173
RseI	CAYNNNNRTG	3 cut(s)	293, 1208, 1938
SapI	GCTCTTC	1 cut(s)	380
SaqAI	TTAA	8 cut(s)	119, 432, 923, 1092, 1269, 1386, 1485, 1958
SatI	GCNGC	5 cut(s)	669, 1167, 1473, 1557, 1735
Sau3AI	GATC	8 cut(s)	313, 650, 909, 1543, 1841, 1849, 2265, 2316
Sau96I	GGNCC	8 cut(s)	159, 463, 679, 1361, 1417, 1678, 1768, 2261
ScaI	AGTACT	1 cut(s)	2174
SchI	GAGTC	2 cut(s)	1112, 1661
ScrFI	CCNGG	2 cut(s)	1422, 1938
SduI	GDGCHC	3 cut(s)	337, 1242, 2026
SfaNI	GCATC	3 cut(s)	47, 1975, 2128
SfcI	CTRYAG	2 cut(s)	1035, 1557
SfoI	GGCGCC	1 cut(s)	1290
SinI	GGWCC	5 cut(s)	463, 679, 1417, 1768, 2261
SmiMI	CAYNNNNRTG	3 cut(s)	293, 1208, 1938
SmlI	CTYRAG	2 cut(s)	1957, 1990
SmoI	CTYRAG	2 cut(s)	1957, 1990
SpeI	ACTAGT	2 cut(s)	202, 1480
SphI	GCATGC	1 cut(s)	1639
SsiI	CCGC	3 cut(s)	1339, 1415, 1734
SspDI	GGCGCC	1 cut(s)	1288
SspMI	CTAG	7 cut(s)	203, 389, 471, 773, 1481, 2001, 2348
StyD4I	CCNGG	2 cut(s)	1420, 1936
StyI	CCWWGG	3 cut(s)	459, 595, 872
TaiI	ACGT	1 cut(s)	499
TaqI	TCGA	3 cut(s)	42, 1670, 1860
TatI	WGTACW	3 cut(s)	258, 487, 2172
TauI	GCSGC	1 cut(s)	1737
TfiI	GAWTC	3 cut(s)	39, 861, 1425
Tru1I	TTAA	8 cut(s)	119, 432, 923, 1092, 1269, 1386, 1485, 1958
Tru9I	TTAA	8 cut(s)	119, 432, 923, 1092, 1269, 1386, 1485, 1958
TscAI	CASTG	6 cut(s)	34, 838, 1071, 1210, 1558, 2338
TseFI	GTSAC	4 cut(s)	1305, 1513, 1528, 1549
TseI	GCWGC	4 cut(s)	668, 1166, 1472, 1556
Tsp45I	GTSAC	4 cut(s)	1305, 1513, 1528, 1549
TspGWI	ACGGA	1 cut(s)	1780
TspRI	CASTG	6 cut(s)	34, 838, 1071, 1210, 1558, 2338
Vha464I	CTTAAG	1 cut(s)	1957
VneI	GTGCAC	1 cut(s)	1238
VpaK11BI	GGWCC	5 cut(s)	463, 679, 1417, 1768, 2261
XapI	RAATTY	4 cut(s)	21, 1369, 1797, 2291
XceI	RCATGY	3 cut(s)	292, 1639, 2087
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1207, 2330
XmiI	GTMKAC	3 cut(s)	1806, 1971, 1996
XmnI	GAANNNNTTC	1 cut(s)	562
XspI	CTAG	7 cut(s)	203, 389, 471, 773, 1481, 2001, 2348
ZrmI	AGTACT	1 cut(s)	2174
Zsp2I	ATGCAT	2 cut(s)	564, 1655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.