FvH4_7g02330

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016911
Physical Location & Seq
Reverse (-)
2642620 .. 2643162
543 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02330.t1

Sequence Viewer

Length: 543 bp
ATGTCTTCGTTTCCTTTCTTCATTCTCTTCACAATCTTCTCCACCTTTTTCCTTTCCACTGATGGAGTTCTAATCAGGCAATTCTTCAAGGCAGATCAGAATAAAGATGAAACGAGCCACCTCCACTTTTTCTTCCATGACATTCTTAGTGGCAAAACTCCAAGTGCTGTGAGAATTATCGGCTCGCAAAACAGCGGCTTCGGTAGTACCTTGATGATTGATGATGCACTCACCGAAAAGCAAGATCCCAAGTCAAAGATCATCGGACGAGCGCAAGGATTTTACTCCGTGGCTGCACAACAGGAGTTTGCACTACTTATGGTCATGAATTTTGTGTTTGTGGAGGGCAAGTACAAGGGGAGTAGCATTAGCATTCTCGGGAGGAATCCAGTGATGAACGATGTGAGGGAGATGCCGATTGTTGGAGGCACCGGATTGTTTCGGTTTGCTCGTGGCTATGTTTTAGCACATACGGTTTGGTTTGATGCAAATACAGGAGACGCCATTGTTGAGTACAATGTTTATGTGTCACAGACCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

181

Amino Acids

20.1

Weight (kDa)

6.4

Isoelectric Point (pI)

28.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 39 - 176 1.9e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016911)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02330
malus_domestica MD07G1030400.v1.1
prunus_persica Prupe.2G028800_v2.0.a1 Prupe.2G028900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0319661
rosa_laevigata RLG00000030466
rosa_roxburghii Rroxscaffold_4G00328230
rosa_rugosa Rorug01G0029400
rosa_samantha Rh1AG041000 Rh1BG038200 Rh1CG042100 Rh1DG027800
rosa_wichuraiana Rw0G019960 Rw1G003410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 428
AciI CCGC 1 cut(s) 195
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 1 cut(s) 328
AcyI GRCGYC 1 cut(s) 501
AfaI GTAC 3 cut(s) 208, 353, 515
AfiI CCNNNNNNNGG 1 cut(s) 422
AgsI TTSAA 1 cut(s) 88
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 377
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 328
AspLEI GCGC 1 cut(s) 274
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 377
BanI GGYRCC 1 cut(s) 428
BauI CACGAG 1 cut(s) 450
BbvI GCAGC 1 cut(s) 280
BccI CCATC 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 492
BisI GCNGC 2 cut(s) 196, 294
BlsI GCNGC 2 cut(s) 197, 295
BmeT110I CYCGRG 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 430
BmsI GCATC 3 cut(s) 214, 402, 475
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 422
Bse1I ACTGG 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 288
BseLI CCNNNNNNNGG 1 cut(s) 422
BseNI ACTGG 1 cut(s) 389
BseXI GCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 428
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 432
BslI CCNNNNNNNGG 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 492
BsmBI CGTCTC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 372
BsoBI CYCGRG 1 cut(s) 377
Bsp143I GATC 3 cut(s) 94, 244, 258
BspACI CCGC 1 cut(s) 195
BspHI TCATGA 2 cut(s) 324, 539
BspLI GGNNCC 1 cut(s) 430
BspPI GGATC 1 cut(s) 239
BspT107I GGYRCC 1 cut(s) 428
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 3 cut(s) 94, 244, 258
BssNI GRCGYC 1 cut(s) 501
BssSI CACGAG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 1 cut(s) 501
BstC8I GCNNGC 1 cut(s) 185
BstDEI CTNAG 1 cut(s) 146
BstDSI CCRYGG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 3 cut(s) 97, 247, 261
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 3 cut(s) 94, 244, 258
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BtgI CCRYGG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 57, 396
Cac8I GCNNGC 1 cut(s) 185
CciI TCATGA 2 cut(s) 324, 539
CfoI GCGC 1 cut(s) 274
CseI GACGC 1 cut(s) 509
Csp6I GTAC 3 cut(s) 207, 352, 514
CviAII CATG 3 cut(s) 137, 325, 540
CviJI RGCY 5 cut(s) 117, 183, 198, 293, 456
CviKI_1 RGCY 5 cut(s) 117, 183, 198, 293, 456
CviQI GTAC 3 cut(s) 207, 352, 514
DdeI CTNAG 1 cut(s) 146
DpnI GATC 3 cut(s) 96, 246, 260
DpnII GATC 3 cut(s) 94, 244, 258
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 492
FaeI CATG 3 cut(s) 140, 328, 543
FaiI YATR 7 cut(s) 138, 320, 326, 459, 471, 525, 541
FatI CATG 3 cut(s) 136, 324, 539
Fnu4HI GCNGC 2 cut(s) 196, 294
Fsp4HI GCNGC 2 cut(s) 196, 294
GlaI GCGC 1 cut(s) 273
GluI GCNGC 2 cut(s) 196, 294
HapII CCGG 1 cut(s) 432
HgaI GACGC 1 cut(s) 509
HhaI GCGC 1 cut(s) 274
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 3 cut(s) 140, 328, 543
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 432
HphI GGTGA 1 cut(s) 223
Hpy188I TCNGA 2 cut(s) 99, 266
Hpy188III TCNNGA 3 cut(s) 325, 379, 540
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4V TGCA 4 cut(s) 227, 296, 311, 488
HpyF3I CTNAG 1 cut(s) 146
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 3 cut(s) 140, 328, 543
HspAI GCGC 1 cut(s) 272
Kzo9I GATC 3 cut(s) 94, 244, 258
LpnPI CCDG 5 cut(s) 61, 287, 402, 445, 480
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 3 cut(s) 214, 402, 475
MaeIII GTNAC 1 cut(s) 528
MalI GATC 3 cut(s) 96, 246, 260
MboI GATC 3 cut(s) 94, 244, 258
MboII GAAGA 5 cut(s) 10, 19, 28, 76, 124
MflI RGATCY 1 cut(s) 244
MluCI AATT 3 cut(s) 80, 174, 328
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 5 cut(s) 131, 337, 375, 399, 419
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 372
NdeII GATC 3 cut(s) 94, 244, 258
NlaIII CATG 3 cut(s) 140, 328, 543
NlaIV GGNNCC 1 cut(s) 430
NmuCI GTSAC 1 cut(s) 528
PagI TCATGA 2 cut(s) 324, 539
PcsI WCGNNNNNNNCGW 1 cut(s) 448
PctI GAATGC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 2 cut(s) 197, 295
PspN4I GGNNCC 1 cut(s) 430
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 3 cut(s) 208, 353, 515
RsaNI GTAC 3 cut(s) 207, 352, 514
SatI GCNGC 2 cut(s) 196, 294
Sau3AI GATC 3 cut(s) 94, 244, 258
SetI ASST 4 cut(s) 47, 123, 212, 539
SfaNI GCATC 3 cut(s) 214, 402, 475
Sse9I AATT 3 cut(s) 80, 174, 328
SsiI CCGC 1 cut(s) 195
TaaI ACNGT 1 cut(s) 475
TasI AATT 3 cut(s) 80, 174, 328
TatI WGTACW 2 cut(s) 351, 513
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 1 cut(s) 385
TscAI CASTG 2 cut(s) 64, 396
TseFI GTSAC 1 cut(s) 528
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 528
TspDTI ATGAA 4 cut(s) 10, 123, 341, 410
TspGWI ACGGA 1 cut(s) 277
TspRI CASTG 2 cut(s) 64, 396
XapI RAATTY 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.