Prupe.2G028800_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0016911
Physical Location & Seq
Reverse (-)
3000938 .. 3001489
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G028800.1

Sequence Viewer

Length: 552 bp
ATGTCTTCATTTCAGTATTTCACTCTCTTCACCCTCTACTTTACCATTTTCTATTCCATGGATGGAATTGAGGTTAGGAAAATCATCAAATCCCAGCAAACCGAAAAAAAGAGCCACCTCCACTTTTTCTTCCATGACATCCTTAGTGGCAAAAACCCTAGTGCCGTTAGAATTGCTGGGCCACTAAACAGTACCATTACTACCTTTGGTGCCACCATGATGATTGATGATGCGCTGACTGAAAAGCAAGAACCTACCTCAAAGATCGTTGGAAGGGCACAAGGGCTGTACTTAGTGGCTGCACAACGTGATCATGAGTTTGCACTGCTTGTGGTTATGAATTTTGCGTTTGTGGAAGGAAAGTACAAGGGAAGTACCATTAGCATCCTTGGGCGGAATTCAGTTCTAAACGATGTGAGAGAGATGCCGATCATCGGAGGAACGGGCCTATTTAGGTTTGCCCGTGGCTATGCCTTAGCCCATACAGTTAGGTTTAATCCTACAGGAGACGCCATTGTTGAGTACAATGTTTTTGTGTCACACTTCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

184

Amino Acids

20.58

Weight (kDa)

9.15

Isoelectric Point (pI)

22.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016911)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02330
malus_domestica MD07G1030400.v1.1
prunus_persica Prupe.2G028800_v2.0.a1 Prupe.2G028900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0319661
rosa_laevigata RLG00000030466
rosa_roxburghii Rroxscaffold_4G00328230
rosa_rugosa Rorug01G0029400
rosa_samantha Rh1AG041000 Rh1BG038200 Rh1CG042100 Rh1DG027800
rosa_wichuraiana Rw0G019960 Rw1G003410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AciI CCGC 1 cut(s) 394
AcsI RAATTY 2 cut(s) 340, 397
AcyI GRCGYC 1 cut(s) 510
AdeI CACNNNGTG 1 cut(s) 308
AfaI GTAC 5 cut(s) 193, 290, 365, 376, 524
AfiI CCNNNNNNNGG 1 cut(s) 434
Alw26I GTCTC 1 cut(s) 501
AoxI GGCC 2 cut(s) 179, 445
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 2 cut(s) 340, 397
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 2 cut(s) 179, 445
AsuHPI GGTGA 1 cut(s) 22
BaeGI GKGCMC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 286
BccI CCATC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 149
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 501
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 501
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 179, 445
BmiI GGNNCC 1 cut(s) 211
BmsI GCATC 3 cut(s) 220, 393, 414
Bpu10I CCTNAGC 1 cut(s) 475
BsaBI GATNNNNATC 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 510
BsaJI CCNNGG 3 cut(s) 57, 388, 463
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 547
Bse8I GATNNNNATC 1 cut(s) 428
BseDI CCNNGG 3 cut(s) 57, 388, 463
BseGI GGATG 3 cut(s) 67, 138, 384
BseJI GATNNNNATC 1 cut(s) 428
BseLI CCNNNNNNNGG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 547
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 1 cut(s) 286
BseYI CCCAGC 2 cut(s) 93, 176
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 2 cut(s) 181, 447
BshNI GGYRCC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 434
BsmAI GTCTC 1 cut(s) 501
BsmBI CGTCTC 1 cut(s) 501
BsnI GGCC 2 cut(s) 181, 447
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 3 cut(s) 264, 310, 429
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 1 cut(s) 394
BspANI GGCC 2 cut(s) 181, 447
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 211
BspT107I GGYRCC 1 cut(s) 209
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 57, 388, 463
BssMI GATC 3 cut(s) 264, 310, 429
BssNI GRCGYC 1 cut(s) 510
BssT1I CCWWGG 2 cut(s) 57, 388
Bst4CI ACNGT 2 cut(s) 191, 487
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 1 cut(s) 510
BstDEI CTNAG 3 cut(s) 143, 292, 475
BstDSI CCRYGG 2 cut(s) 57, 463
BstF5I GGATG 3 cut(s) 67, 138, 384
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 3 cut(s) 267, 313, 432
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 3 cut(s) 264, 310, 429
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 286
BsuRI GGCC 2 cut(s) 181, 447
BtgI CCRYGG 2 cut(s) 57, 463
BtsCI GGATG 3 cut(s) 67, 138, 384
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 1 cut(s) 323
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 179, 445
CseI GACGC 1 cut(s) 518
Csp6I GTAC 5 cut(s) 192, 289, 364, 375, 523
CviAII CATG 4 cut(s) 58, 134, 217, 314
CviJI RGCY 7 cut(s) 114, 181, 286, 299, 447, 468, 479
CviKI_1 RGCY 7 cut(s) 114, 181, 286, 299, 447, 468, 479
CviQI GTAC 5 cut(s) 192, 289, 364, 375, 523
DdeI CTNAG 3 cut(s) 143, 292, 475
DpnI GATC 3 cut(s) 266, 312, 431
DpnII GATC 3 cut(s) 264, 310, 429
DraIII CACNNNGTG 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 409
Eco130I CCWWGG 2 cut(s) 57, 388
EcoRI GAATTC 1 cut(s) 397
EcoT14I CCWWGG 2 cut(s) 57, 388
ErhI CCWWGG 2 cut(s) 57, 388
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 4 cut(s) 61, 137, 220, 317
FaiI YATR 7 cut(s) 59, 135, 218, 315, 338, 471, 483
FatI CATG 4 cut(s) 57, 133, 216, 313
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 300
FokI GGATG 3 cut(s) 74, 125, 371
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 300
GsaI CCCAGC 2 cut(s) 97, 180
HaeIII GGCC 2 cut(s) 181, 447
HgaI GACGC 1 cut(s) 518
HhaI GCGC 1 cut(s) 235
Hin1I GRCGYC 1 cut(s) 510
Hin1II CATG 4 cut(s) 61, 137, 220, 317
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HphI GGTGA 1 cut(s) 22
Hpy188I TCNGA 1 cut(s) 437
Hpy188III TCNNGA 1 cut(s) 314
HpyAV CCTTC 2 cut(s) 267, 350
HpyCH4III ACNGT 2 cut(s) 191, 487
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 2 cut(s) 302, 323
HpyF3I CTNAG 3 cut(s) 143, 292, 475
HpySE526I ACGT 1 cut(s) 307
Hsp92I GRCGYC 1 cut(s) 510
Hsp92II CATG 4 cut(s) 61, 137, 220, 317
HspAI GCGC 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 3 cut(s) 264, 310, 429
LpnPI CCDG 3 cut(s) 107, 162, 489
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 3 cut(s) 220, 393, 414
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 1 cut(s) 537
MalI GATC 3 cut(s) 266, 312, 431
MboI GATC 3 cut(s) 264, 310, 429
MboII GAAGA 2 cut(s) 19, 121
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 4 cut(s) 66, 171, 340, 397
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 5 cut(s) 44, 64, 128, 268, 431
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 1 cut(s) 218
NcoI CCATGG 1 cut(s) 57
NdeII GATC 3 cut(s) 264, 310, 429
NlaIII CATG 4 cut(s) 61, 137, 220, 317
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 537
PagI TCATGA 1 cut(s) 313
PkrI GCNGC 1 cut(s) 301
PspFI CCCAGC 2 cut(s) 93, 176
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 2 cut(s) 179, 445
RsaI GTAC 5 cut(s) 193, 290, 365, 376, 524
RsaNI GTAC 5 cut(s) 192, 289, 364, 375, 523
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 3 cut(s) 264, 310, 429
Sau96I GGNCC 2 cut(s) 179, 445
SduI GDGCHC 1 cut(s) 280
SetI ASST 8 cut(s) 75, 120, 206, 256, 260, 310, 458, 494
SfaNI GCATC 3 cut(s) 220, 393, 414
SfcI CTRYAG 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 4 cut(s) 66, 171, 340, 397
SsiI CCGC 1 cut(s) 394
SspMI CTAG 1 cut(s) 159
StyI CCWWGG 2 cut(s) 57, 388
TaaI ACNGT 2 cut(s) 191, 487
TaiI ACGT 1 cut(s) 310
TasI AATT 4 cut(s) 66, 171, 340, 397
TatI WGTACW 3 cut(s) 288, 363, 522
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 1 cut(s) 537
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 1 cut(s) 537
TspDTI ATGAA 1 cut(s) 353
TspRI CASTG 1 cut(s) 330
XapI RAATTY 2 cut(s) 340, 397
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.