RchiOBHm_Chr1g0319661

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016911
Physical Location & Seq
Reverse (-)
7291691 .. 7292519
829 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54988

Sequence Viewer

Length: 564 bp
ATGCATACTATGTCCTCATTTCTTCTCGTCACTCTCTTCACAATCTTCTCCACCTTTTTATTCTCCATTGATGGAGTTCTAATCAGGCAATTCTTCAAGGCAGATCAGAAAAAAGAAGAAGAAACGAGCCACCTTCACTTTTTCTTCCATGACATTCTTAGTGGCAAAACTCCAAGTGCTGTCAGAATTATCGGCGGGCCAAACAGCAGCCTCTCCAGCTTCGGTTCCACCTTGATGATTGATGATGCGCTCACTGAAAAGCAAGATCCCACATCGAAGATCATCGGACGAGCACAAGGGTTTTACTCAGTGGCTGCACAACAGGAGATTGCACTGCTTATGGTCATGAGTTTTCAGTTTGTGGAGGGTCAGTACAAGGGGAGTAGCATCAGCATTCTTGGGAGGAATCCAGTGATGAACGATGTCAGGGAGATGCCGATTGTTGGAGGCACCGGATTGTTTCGGTTTGCTCGTGGCTATGTTTTAGCACATACGGTTTGGTTTGATGCTAATACAGGAGACGCCATTGTTGAGTACAATGTTTATGTGTCACAGACCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

187

Amino Acids

20.76

Weight (kDa)

5.92

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 43 - 183 4.5e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016911)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02330
malus_domestica MD07G1030400.v1.1
prunus_persica Prupe.2G028800_v2.0.a1 Prupe.2G028900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0319661
rosa_laevigata RLG00000030466
rosa_roxburghii Rroxscaffold_4G00328230
rosa_rugosa Rorug01G0029400
rosa_samantha Rh1AG041000 Rh1BG038200 Rh1CG042100 Rh1DG027800
rosa_wichuraiana Rw0G019960 Rw1G003410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 449
AciI CCGC 1 cut(s) 195
AclWI GGATC 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 522
AfaI GTAC 2 cut(s) 374, 536
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 1 cut(s) 97
AluBI AGCT 1 cut(s) 219
AluI AGCT 1 cut(s) 219
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 1 cut(s) 513
AlwI GGATC 1 cut(s) 260
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 2 cut(s) 207, 314
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 449
BauI CACGAG 2 cut(s) 471, 559
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 219, 301
BccI CCATC 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 513
BisI GCNGC 2 cut(s) 208, 315
BlsI GCNGC 2 cut(s) 209, 316
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 226, 451
BmsI GCATC 4 cut(s) 235, 396, 423, 496
BpmI CTGGAG 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 452
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 1 cut(s) 410
BseXI GCAGC 2 cut(s) 219, 301
BsgI GTGCAG 1 cut(s) 300
BshFI GGCC 1 cut(s) 199
BshNI GGYRCC 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 453
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 513
BsmBI CGTCTC 1 cut(s) 513
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 3 cut(s) 103, 265, 279
BspACI CCGC 1 cut(s) 195
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 1 cut(s) 320
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 226, 451
BspPI GGATC 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 449
BsrI ACTGG 1 cut(s) 410
BssMI GATC 3 cut(s) 103, 265, 279
BssNI GRCGYC 1 cut(s) 522
BssSI CACGAG 2 cut(s) 471, 559
Bst2BI CACGAG 2 cut(s) 471, 559
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 2 cut(s) 158, 307
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 3 cut(s) 106, 268, 282
BstMAI GTCTC 1 cut(s) 513
BstMBI GATC 3 cut(s) 103, 265, 279
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 219, 301
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 332
BtsIMutI CAGTG 4 cut(s) 252, 315, 332, 417
Cac8I GCNNGC 1 cut(s) 197
CaiI CAGNNNCTG 1 cut(s) 314
CciI TCATGA 1 cut(s) 345
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 197
CseI GACGC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 373, 535
CviAII CATG 2 cut(s) 149, 346
CviJI RGCY 6 cut(s) 129, 199, 210, 219, 314, 477
CviKI_1 RGCY 6 cut(s) 129, 199, 210, 219, 314, 477
CviQI GTAC 2 cut(s) 373, 535
DdeI CTNAG 2 cut(s) 158, 307
DpnI GATC 3 cut(s) 105, 267, 281
DpnII GATC 3 cut(s) 103, 265, 279
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 513
FaeI CATG 2 cut(s) 152, 349
FaiI YATR 8 cut(s) 6, 11, 150, 341, 347, 480, 492, 546
FatI CATG 2 cut(s) 148, 345
FauI CCCGC 1 cut(s) 188
Fnu4HI GCNGC 2 cut(s) 208, 315
Fsp4HI GCNGC 2 cut(s) 208, 315
GlaI GCGC 1 cut(s) 249
GluI GCNGC 2 cut(s) 208, 315
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 1 cut(s) 199
HapII CCGG 1 cut(s) 453
HgaI GACGC 1 cut(s) 530
HhaI GCGC 1 cut(s) 250
Hin1I GRCGYC 1 cut(s) 522
Hin1II CATG 2 cut(s) 152, 349
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 1 cut(s) 406
HpaII CCGG 1 cut(s) 453
Hpy188I TCNGA 3 cut(s) 108, 185, 287
Hpy188III TCNNGA 2 cut(s) 346, 561
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4V TGCA 3 cut(s) 4, 317, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 2 cut(s) 158, 307
Hsp92I GRCGYC 1 cut(s) 522
Hsp92II CATG 2 cut(s) 152, 349
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 3 cut(s) 103, 265, 279
LpnPI CCDG 7 cut(s) 70, 229, 308, 412, 423, 466, 501
Lsp1109I GCAGC 2 cut(s) 219, 301
LweI GCATC 4 cut(s) 235, 396, 423, 496
MaeIII GTNAC 2 cut(s) 28, 549
MalI GATC 3 cut(s) 105, 267, 281
MboI GATC 3 cut(s) 103, 265, 279
MboII GAAGA 8 cut(s) 14, 28, 37, 85, 128, 131, 136, 289
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 2 cut(s) 89, 186
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 5 cut(s) 25, 221, 358, 396, 440
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 233
MspI CCGG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 393
MwoI GCNNNNNNNGC 1 cut(s) 216
NdeII GATC 3 cut(s) 103, 265, 279
NlaIII CATG 2 cut(s) 152, 349
NlaIV GGNNCC 2 cut(s) 226, 451
NmuCI GTSAC 2 cut(s) 28, 549
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 469
PctI GAATGC 1 cut(s) 393
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 2 cut(s) 209, 316
PspN4I GGNNCC 2 cut(s) 226, 451
PspPI GGNCC 1 cut(s) 197
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 2 cut(s) 374, 536
RsaNI GTAC 2 cut(s) 373, 535
RseI CAYNNNNRTG 1 cut(s) 233
SatI GCNGC 2 cut(s) 208, 315
Sau3AI GATC 3 cut(s) 103, 265, 279
Sau96I GGNCC 1 cut(s) 197
SduI GDGCHC 1 cut(s) 295
SetI ASST 5 cut(s) 56, 135, 221, 233, 560
SfaNI GCATC 4 cut(s) 235, 396, 423, 496
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 2 cut(s) 89, 186
SsiI CCGC 1 cut(s) 195
TaaI ACNGT 1 cut(s) 496
TaqI TCGA 1 cut(s) 275
TasI AATT 2 cut(s) 89, 186
TatI WGTACW 2 cut(s) 372, 534
TfiI GAWTC 1 cut(s) 406
TscAI CASTG 4 cut(s) 259, 315, 339, 417
TseFI GTSAC 2 cut(s) 28, 549
TseI GCWGC 2 cut(s) 207, 314
Tsp45I GTSAC 2 cut(s) 28, 549
TspDTI ATGAA 1 cut(s) 431
TspRI CASTG 4 cut(s) 259, 315, 339, 417
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.