RLG00000030466

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016911
Physical Location & Seq
Forward (+)
62128802 .. 62130055
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030466

Sequence Viewer

Length: 561 bp
ATGCATACTATGTCCTCATTTCTTCTCTTCATTCTCTTCACAATTTTCTCCACCTTTTTCTTCTCCATTGATGGAGTTCTAATCAGGCAATTCTTCAAGGCAGATCAGAAAAAAGAAGAAGAAACGCACCTCCACTTTTTCTTCCATGACATTCTTAGTGGCAAAAGTCCAAGTGCTGTCAGAATTATCGGCGCGCCAAACAGCAGCCTCTCCAGCTTCGGTTCCACCTTGATGATTGATGATGCACTCACTGAAAAGCAAGATCCCACATCGAAGATCATTGGACGAGCACAAGGGTTTTACTCAGTGGCTGCACAACAGGAGATTTCACTGCTTATGGTCATGAGTTTTCAGTTTGTGGAGGGTCAGTACAAGGGGAGTAGCATCAGCATTCTTGGGAGGAATCCAGTGATGAACGATGTCAGGGAGATGCCGATTGTTGGAGGCACCGGATTGTTTCGGTTTGCTCGTGGCTATGTTTTAGCACATACGGTTTGGTTTGATGCTAATACAGGAGACGCCATTGTTGAGTACAATGTTTATGTGTCACAGACCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

187

Amino Acids

20.78

Weight (kDa)

5.92

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 182 1.4e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016911)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02330
malus_domestica MD07G1030400.v1.1
prunus_persica Prupe.2G028800_v2.0.a1 Prupe.2G028900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0319661
rosa_laevigata RLG00000030466
rosa_roxburghii Rroxscaffold_4G00328230
rosa_rugosa Rorug01G0029400
rosa_samantha Rh1AG041000 Rh1BG038200 Rh1CG042100 Rh1DG027800
rosa_wichuraiana Rw0G019960 Rw1G003410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 446
AccII CGCG 1 cut(s) 194
AclWI GGATC 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 2 cut(s) 371, 533
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 1 cut(s) 97
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 311
ApeKI GCWGC 2 cut(s) 204, 311
AscI GGCGCGCC 1 cut(s) 192
AspLEI GCGC 2 cut(s) 194, 196
BanI GGYRCC 1 cut(s) 446
BauI CACGAG 2 cut(s) 468, 556
Bbv12I GWGCWC 1 cut(s) 292
BbvI GCAGC 2 cut(s) 216, 298
BccI CCATC 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 510
BisI GCNGC 2 cut(s) 205, 312
BlsI GCNGC 2 cut(s) 206, 313
BmiI GGNNCC 2 cut(s) 223, 448
BmsI GCATC 4 cut(s) 232, 393, 420, 493
BpmI CTGGAG 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 519
BsaWI WCCGGW 1 cut(s) 449
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse1I ACTGG 1 cut(s) 407
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 1 cut(s) 407
BsePI GCGCGC 1 cut(s) 192
BseXI GCAGC 2 cut(s) 216, 298
BsgI GTGCAG 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 292
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 440
BsmAI GTCTC 1 cut(s) 510
BsmBI CGTCTC 1 cut(s) 510
BsmI GAATGC 1 cut(s) 390
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 3 cut(s) 103, 262, 276
BspCNI CTCAG 1 cut(s) 317
BspFNI CGCG 1 cut(s) 194
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 223, 448
BspPI GGATC 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 446
BsrI ACTGG 1 cut(s) 407
BssHII GCGCGC 1 cut(s) 192
BssMI GATC 3 cut(s) 103, 262, 276
BssNI GRCGYC 1 cut(s) 519
BssSI CACGAG 2 cut(s) 468, 556
Bst2BI CACGAG 2 cut(s) 468, 556
Bst4CI ACNGT 1 cut(s) 493
Bst6I CTCTTC 2 cut(s) 32, 41
BstACI GRCGYC 1 cut(s) 519
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 2 cut(s) 155, 304
BstFNI CGCG 1 cut(s) 194
BstHHI GCGC 2 cut(s) 194, 196
BstKTI GATC 3 cut(s) 106, 265, 279
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 3 cut(s) 103, 262, 276
BstMWI GCNNNNNNNGC 1 cut(s) 213
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 216, 298
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
BtsI GCAGTG 1 cut(s) 329
BtsIMutI CAGTG 4 cut(s) 249, 312, 329, 414
Cac8I GCNNGC 1 cut(s) 194
CaiI CAGNNNCTG 1 cut(s) 311
CciI TCATGA 1 cut(s) 342
CfoI GCGC 2 cut(s) 194, 196
CseI GACGC 1 cut(s) 527
Csp6I GTAC 2 cut(s) 370, 532
CviAII CATG 2 cut(s) 146, 343
CviJI RGCY 4 cut(s) 207, 216, 311, 474
CviKI_1 RGCY 4 cut(s) 207, 216, 311, 474
CviQI GTAC 2 cut(s) 370, 532
DdeI CTNAG 2 cut(s) 155, 304
DpnI GATC 3 cut(s) 105, 264, 278
DpnII GATC 3 cut(s) 103, 262, 276
Eam1104I CTCTTC 2 cut(s) 32, 41
EarI CTCTTC 2 cut(s) 32, 41
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 510
FaeI CATG 2 cut(s) 149, 346
FaiI YATR 8 cut(s) 6, 11, 147, 338, 344, 477, 489, 543
FatI CATG 2 cut(s) 145, 342
Fnu4HI GCNGC 2 cut(s) 205, 312
Fsp4HI GCNGC 2 cut(s) 205, 312
GlaI GCGC 2 cut(s) 193, 195
GluI GCNGC 2 cut(s) 205, 312
GsuI CTGGAG 1 cut(s) 196
HapII CCGG 1 cut(s) 450
HgaI GACGC 1 cut(s) 527
HhaI GCGC 2 cut(s) 194, 196
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 2 cut(s) 149, 346
Hin6I GCGC 2 cut(s) 192, 194
HinP1I GCGC 2 cut(s) 192, 194
HinfI GANTC 1 cut(s) 403
HpaII CCGG 1 cut(s) 450
Hpy188I TCNGA 2 cut(s) 108, 182
Hpy188III TCNNGA 2 cut(s) 343, 558
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4V TGCA 3 cut(s) 4, 245, 314
HpyF10VI GCNNNNNNNGC 1 cut(s) 213
HpyF3I CTNAG 2 cut(s) 155, 304
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 2 cut(s) 149, 346
HspAI GCGC 2 cut(s) 192, 194
Kzo9I GATC 3 cut(s) 103, 262, 276
LpnPI CCDG 7 cut(s) 70, 226, 305, 409, 420, 463, 498
Lsp1109I GCAGC 2 cut(s) 216, 298
LweI GCATC 4 cut(s) 232, 393, 420, 493
MaeIII GTNAC 1 cut(s) 546
MalI GATC 3 cut(s) 105, 264, 278
MboI GATC 3 cut(s) 103, 262, 276
MboII GAAGA 9 cut(s) 14, 19, 28, 52, 85, 128, 131, 133, 286
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 3 cut(s) 42, 89, 183
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 6 cut(s) 25, 140, 218, 355, 393, 437
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 230
MspI CCGG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 390
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 213
NdeII GATC 3 cut(s) 103, 262, 276
NlaIII CATG 2 cut(s) 149, 346
NlaIV GGNNCC 2 cut(s) 223, 448
NmuCI GTSAC 1 cut(s) 546
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 342
PalAI GGCGCGCC 1 cut(s) 192
PauI GCGCGC 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 466
PctI GAATGC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 403
PkrI GCNGC 2 cut(s) 206, 313
PspN4I GGNNCC 2 cut(s) 223, 448
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 1 cut(s) 262
PteI GCGCGC 1 cut(s) 192
RsaI GTAC 2 cut(s) 371, 533
RsaNI GTAC 2 cut(s) 370, 532
RseI CAYNNNNRTG 1 cut(s) 230
SatI GCNGC 2 cut(s) 205, 312
Sau3AI GATC 3 cut(s) 103, 262, 276
SduI GDGCHC 1 cut(s) 292
SetI ASST 5 cut(s) 56, 132, 218, 230, 557
SfaNI GCATC 4 cut(s) 232, 393, 420, 493
SgsI GGCGCGCC 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 3 cut(s) 42, 89, 183
TaaI ACNGT 1 cut(s) 493
TaqI TCGA 1 cut(s) 272
TasI AATT 3 cut(s) 42, 89, 183
TatI WGTACW 2 cut(s) 369, 531
TfiI GAWTC 1 cut(s) 403
TscAI CASTG 4 cut(s) 256, 312, 336, 414
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 2 cut(s) 204, 311
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 2 cut(s) 19, 428
TspRI CASTG 4 cut(s) 256, 312, 336, 414
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.