Rroxscaffold_4G00328230

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016911
Physical Location & Seq
Forward (+)
61143615 .. 61145946
2332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328230.1

Sequence Viewer

Length: 660 bp
ATGCATGGTGGCGGCGGCGGCGATTTGCTTGGCGTGGTGGAAATCGTAGGGTGCCCTCGGCAATTCGACCCATTGGGTCGGCACGAGTTGGTGGGTTTGAAGAATGGCTGGATGTGGGTATGTAGGGTCCAAAGTCTTCTTAGGGCCTGGTCTGTCTTTGGAATAGGGGTGGAGGTTCTAATCAGGCAATTCTTCAAGGCAGATCAGAAAAAAGAAGAAGAAACGCACCTCCACTTTTTCTTCCATGACATTCTTAGTGGCAAAAGTCCAAGTGCTGTCAGAATTATCGGCGCGCCAAACAGCAGCCTCTCCAGCTTCGGTTCCACCTTGATGATTGATGATGCGCTCACTGAAAAGCAAGATCCCACATCGAAGATCATTGGACGAGCACAAGGGTTTTACTCAGTGGCTGCACAACAGGAGATTGCACTGCTTATGGTCATGAGTTTTCAGTTTGTGGAGGGTCAGTACAAGGGGAGTAGCATCAGCATTCTTGGGAGGAATCCAGTGATGAACGATGTCAGGGAGATGCCAATTGTTGGAGGCACCGGATTGTTTCGGTTTGCTCGTGGCTATGTTTTAGCACATACGGTTTGGTTTGATGCTAATACAGGAGACGCCATTGTTGAGTACAATGTTTATGTGTCACAGACCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

219

Amino Acids

24.1

Weight (kDa)

6.29

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 75 - 215 3.6e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016911)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02330
malus_domestica MD07G1030400.v1.1
prunus_persica Prupe.2G028800_v2.0.a1 Prupe.2G028900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0319661
rosa_laevigata RLG00000030466
rosa_roxburghii Rroxscaffold_4G00328230
rosa_rugosa Rorug01G0029400
rosa_samantha Rh1AG041000 Rh1BG038200 Rh1CG042100 Rh1DG027800
rosa_wichuraiana Rw0G019960 Rw1G003410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 51, 545
AccB7I CCANNNNNTGG 1 cut(s) 539
AccII CGCG 1 cut(s) 293
AciI CCGC 3 cut(s) 12, 15, 18
AclWI GGATC 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 2 cut(s) 470, 632
AfiI CCNNNNNNNGG 1 cut(s) 539
AgsI TTSAA 2 cut(s) 100, 196
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 1 cut(s) 315
AluI AGCT 1 cut(s) 315
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 1 cut(s) 609
AlwI GGATC 1 cut(s) 356
AlwNI CAGNNNCTG 1 cut(s) 410
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 2 cut(s) 303, 410
AscI GGCGCGCC 1 cut(s) 291
AspLEI GCGC 3 cut(s) 293, 295, 346
AspS9I GGNCC 2 cut(s) 127, 144
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 2 cut(s) 51, 545
BauI CACGAG 3 cut(s) 83, 567, 655
BbsI GAAGAC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 2 cut(s) 315, 397
BciT130I CCWGG 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 609
BisI GCNGC 5 cut(s) 13, 16, 19, 304, 411
BlsI GCNGC 5 cut(s) 14, 17, 20, 305, 412
Bme1390I CCNGG 1 cut(s) 148
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 127, 144
BmiI GGNNCC 4 cut(s) 53, 128, 322, 547
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 4 cut(s) 331, 492, 519, 592
BpiI GAAGAC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 1 cut(s) 56
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse1I ACTGG 1 cut(s) 506
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 506
BsePI GCGCGC 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 2 cut(s) 315, 397
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 2 cut(s) 51, 545
BsiHKAI GWGCWC 1 cut(s) 391
BsiSI CCGG 1 cut(s) 549
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 1 cut(s) 609
BsmBI CGTCTC 1 cut(s) 609
BsmI GAATGC 1 cut(s) 489
BsnI GGCC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 56, 391
Bsp143I GATC 3 cut(s) 202, 361, 375
BspACI CCGC 3 cut(s) 12, 15, 18
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 1 cut(s) 416
BspFNI CGCG 1 cut(s) 293
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 4 cut(s) 53, 128, 322, 547
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 2 cut(s) 51, 545
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 1 cut(s) 56
BssHII GCGCGC 1 cut(s) 291
BssMI GATC 3 cut(s) 202, 361, 375
BssNI GRCGYC 1 cut(s) 618
BssSI CACGAG 3 cut(s) 83, 567, 655
Bst2BI CACGAG 3 cut(s) 83, 567, 655
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 1 cut(s) 592
BstACI GRCGYC 1 cut(s) 618
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 3 cut(s) 140, 254, 403
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 1 cut(s) 293
BstHHI GCGC 3 cut(s) 293, 295, 346
BstKTI GATC 3 cut(s) 205, 364, 378
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 3 cut(s) 202, 361, 375
BstMWI GCNNNNNNNGC 2 cut(s) 18, 312
BstNI CCWGG 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 146
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 315, 397
BstV2I GAAGAC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 1 cut(s) 146
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 428
BtsIMutI CAGTG 4 cut(s) 348, 411, 428, 513
Cac8I GCNNGC 1 cut(s) 293
CaiI CAGNNNCTG 1 cut(s) 410
CciI TCATGA 1 cut(s) 441
CfoI GCGC 3 cut(s) 293, 295, 346
Cfr13I GGNCC 2 cut(s) 127, 144
CseI GACGC 1 cut(s) 626
Csp6I GTAC 2 cut(s) 469, 631
CviAII CATG 3 cut(s) 5, 245, 442
CviJI RGCY 6 cut(s) 108, 146, 306, 315, 410, 573
CviKI_1 RGCY 6 cut(s) 108, 146, 306, 315, 410, 573
CviQI GTAC 2 cut(s) 469, 631
DdeI CTNAG 3 cut(s) 140, 254, 403
DpnI GATC 3 cut(s) 204, 363, 377
DpnII GATC 3 cut(s) 202, 361, 375
Eco47I GGWCC 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 609
FaeI CATG 3 cut(s) 8, 248, 445
FaiI YATR 8 cut(s) 6, 121, 246, 437, 443, 576, 588, 642
FatI CATG 3 cut(s) 4, 244, 441
Fnu4HI GCNGC 5 cut(s) 13, 16, 19, 304, 411
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 5 cut(s) 13, 16, 19, 304, 411
GlaI GCGC 3 cut(s) 292, 294, 345
GluI GCNGC 5 cut(s) 13, 16, 19, 304, 411
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 1 cut(s) 146
HapII CCGG 1 cut(s) 549
HgaI GACGC 1 cut(s) 626
HhaI GCGC 3 cut(s) 293, 295, 346
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 3 cut(s) 8, 248, 445
Hin6I GCGC 3 cut(s) 291, 293, 344
HinP1I GCGC 3 cut(s) 291, 293, 344
HinfI GANTC 1 cut(s) 502
HpaII CCGG 1 cut(s) 549
Hpy188I TCNGA 2 cut(s) 207, 281
Hpy188III TCNNGA 2 cut(s) 442, 657
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 3 cut(s) 4, 413, 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 312
HpyF3I CTNAG 3 cut(s) 140, 254, 403
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 3 cut(s) 8, 248, 445
HspAI GCGC 3 cut(s) 291, 293, 344
Kzo9I GATC 3 cut(s) 202, 361, 375
Lsp1109I GCAGC 2 cut(s) 315, 397
LweI GCATC 4 cut(s) 331, 492, 519, 592
MaeIII GTNAC 1 cut(s) 645
MalI GATC 3 cut(s) 204, 363, 377
MboI GATC 3 cut(s) 202, 361, 375
MboII GAAGA 7 cut(s) 112, 128, 184, 227, 230, 232, 385
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 2 cut(s) 56, 391
MluCI AATT 4 cut(s) 62, 188, 282, 534
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 7 cut(s) 66, 166, 239, 317, 454, 492, 536
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 329
MspI CCGG 1 cut(s) 549
MspR9I CCNGG 1 cut(s) 148
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 1 cut(s) 148
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 18, 312
NdeII GATC 3 cut(s) 202, 361, 375
NlaIII CATG 3 cut(s) 8, 248, 445
NlaIV GGNNCC 4 cut(s) 53, 128, 322, 547
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 645
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 441
PalAI GGCGCGCC 1 cut(s) 291
PauI GCGCGC 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 565
PctI GAATGC 1 cut(s) 489
PfeI GAWTC 1 cut(s) 502
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 5 cut(s) 14, 17, 20, 305, 412
Psp6I CCWGG 1 cut(s) 146
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 4 cut(s) 53, 128, 322, 547
PspPI GGNCC 2 cut(s) 127, 144
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 1 cut(s) 361
PteI GCGCGC 1 cut(s) 291
RsaI GTAC 2 cut(s) 470, 632
RsaNI GTAC 2 cut(s) 469, 631
RseI CAYNNNNRTG 1 cut(s) 329
SatI GCNGC 5 cut(s) 13, 16, 19, 304, 411
Sau3AI GATC 3 cut(s) 202, 361, 375
Sau96I GGNCC 2 cut(s) 127, 144
ScrFI CCNGG 1 cut(s) 148
SduI GDGCHC 2 cut(s) 56, 391
SetI ASST 5 cut(s) 177, 231, 317, 329, 656
SfaNI GCATC 4 cut(s) 331, 492, 519, 592
SgsI GGCGCGCC 1 cut(s) 291
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 329
Sse9I AATT 4 cut(s) 62, 188, 282, 534
SsiI CCGC 3 cut(s) 12, 15, 18
StyD4I CCNGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 592
TaqI TCGA 2 cut(s) 66, 371
TasI AATT 4 cut(s) 62, 188, 282, 534
TatI WGTACW 2 cut(s) 468, 630
TauI GCSGC 3 cut(s) 15, 18, 21
TfiI GAWTC 1 cut(s) 502
TscAI CASTG 4 cut(s) 355, 411, 435, 513
TseFI GTSAC 1 cut(s) 645
TseI GCWGC 2 cut(s) 303, 410
Tsp45I GTSAC 1 cut(s) 645
TspDTI ATGAA 1 cut(s) 527
TspRI CASTG 4 cut(s) 355, 411, 435, 513
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.