Rw0G019960

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00902
OG0016911
Physical Location & Seq
Forward (+)
47858 .. 48412
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019960.1

Sequence Viewer

Length: 555 bp
ATGTCCTCATTTCTTCTCTTCACTCTCTTCACAATCTTCTCCACCTTTTTATTCTCCATTGATGGAGTTCTAATCAGGCAATTCTTCAAGGCAGATCAGAAAAAAGAAGAAGAAACGAGCCACCTTCACTTTTTCTTCCATGACATTCTTAGTGGCAAAACTCCAAGTGCTGTCAGAATTATCGGCGGGCCAAACAGCAGCCTCTCCAGCTTCGGTTCCACCTTGATGATTGATGATGCGCTCACTGAAAAGCAAGATCCCACATCGAAGATCATCGGACGAGCACAAGGGTTTTACTCAGTGGCTGCACAACAGGAGATTGCACTGCTTATGGTCATGAGTTTTCAGTTTGTGGAAGGTCAGTACAAGGGGAGTAGCATCAGCATTCTTGGGAGGAATCCAGTGATGAACGATGTCAGGGAGATGCCGATTGTTGGAGGCACCGGATTGTTTCGGTTTGCTCGTGGCTATGTTTTAGCACATACGGTTTGGTTTGATGCTAATACAGGAGACGCCATTGTTGAGTACAATGTTTATGTGTCACAGACCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

184

Amino Acids

20.44

Weight (kDa)

5.76

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 40 - 180 4.3e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016911)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02330
malus_domestica MD07G1030400.v1.1
prunus_persica Prupe.2G028800_v2.0.a1 Prupe.2G028900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0319661
rosa_laevigata RLG00000030466
rosa_roxburghii Rroxscaffold_4G00328230
rosa_rugosa Rorug01G0029400
rosa_samantha Rh1AG041000 Rh1BG038200 Rh1CG042100 Rh1DG027800
rosa_wichuraiana Rw0G019960 Rw1G003410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 440
AciI CCGC 1 cut(s) 186
AclWI GGATC 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 513
AfaI GTAC 2 cut(s) 365, 527
AfiI CCNNNNNNNGG 1 cut(s) 434
AgsI TTSAA 1 cut(s) 88
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 1 cut(s) 251
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 198, 305
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 188
BanI GGYRCC 1 cut(s) 440
BauI CACGAG 2 cut(s) 462, 550
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 2 cut(s) 210, 292
BccI CCATC 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 504
BisI GCNGC 2 cut(s) 199, 306
BlsI GCNGC 2 cut(s) 200, 307
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 2 cut(s) 217, 442
BmsI GCATC 4 cut(s) 226, 387, 414, 487
BpmI CTGGAG 1 cut(s) 190
BsaHI GRCGYC 1 cut(s) 513
BsaWI WCCGGW 1 cut(s) 443
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 401
BseLI CCNNNNNNNGG 1 cut(s) 434
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 401
BseXI GCAGC 2 cut(s) 210, 292
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 1 cut(s) 190
BshNI GGYRCC 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 286
BsiSI CCGG 1 cut(s) 444
BslI CCNNNNNNNGG 1 cut(s) 434
BsmAI GTCTC 1 cut(s) 504
BsmBI CGTCTC 1 cut(s) 504
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 3 cut(s) 94, 256, 270
BspACI CCGC 1 cut(s) 186
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 1 cut(s) 311
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 2 cut(s) 217, 442
BspPI GGATC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 440
BsrI ACTGG 1 cut(s) 401
BssMI GATC 3 cut(s) 94, 256, 270
BssNI GRCGYC 1 cut(s) 513
BssSI CACGAG 2 cut(s) 462, 550
Bst2BI CACGAG 2 cut(s) 462, 550
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 2 cut(s) 23, 32
BstACI GRCGYC 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 2 cut(s) 149, 298
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 3 cut(s) 97, 259, 273
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 3 cut(s) 94, 256, 270
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 210, 292
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 1 cut(s) 190
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 4 cut(s) 243, 306, 323, 408
Cac8I GCNNGC 1 cut(s) 188
CaiI CAGNNNCTG 1 cut(s) 305
CciI TCATGA 1 cut(s) 336
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 188
CseI GACGC 1 cut(s) 521
Csp6I GTAC 2 cut(s) 364, 526
CviAII CATG 2 cut(s) 140, 337
CviJI RGCY 6 cut(s) 120, 190, 201, 210, 305, 468
CviKI_1 RGCY 6 cut(s) 120, 190, 201, 210, 305, 468
CviQI GTAC 2 cut(s) 364, 526
DdeI CTNAG 2 cut(s) 149, 298
DpnI GATC 3 cut(s) 96, 258, 272
DpnII GATC 3 cut(s) 94, 256, 270
Eam1104I CTCTTC 2 cut(s) 23, 32
EarI CTCTTC 2 cut(s) 23, 32
Esp3I CGTCTC 1 cut(s) 504
FaeI CATG 2 cut(s) 143, 340
FaiI YATR 6 cut(s) 141, 332, 338, 471, 483, 537
FatI CATG 2 cut(s) 139, 336
FauI CCCGC 1 cut(s) 179
Fnu4HI GCNGC 2 cut(s) 199, 306
Fsp4HI GCNGC 2 cut(s) 199, 306
GlaI GCGC 1 cut(s) 240
GluI GCNGC 2 cut(s) 199, 306
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 1 cut(s) 190
HapII CCGG 1 cut(s) 444
HgaI GACGC 1 cut(s) 521
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 513
Hin1II CATG 2 cut(s) 143, 340
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HinfI GANTC 1 cut(s) 397
HpaII CCGG 1 cut(s) 444
Hpy188I TCNGA 3 cut(s) 99, 176, 278
Hpy188III TCNNGA 2 cut(s) 337, 552
HpyAV CCTTC 2 cut(s) 134, 350
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4V TGCA 2 cut(s) 308, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 2 cut(s) 149, 298
Hsp92I GRCGYC 1 cut(s) 513
Hsp92II CATG 2 cut(s) 143, 340
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 3 cut(s) 94, 256, 270
LpnPI CCDG 7 cut(s) 61, 220, 299, 403, 414, 457, 492
Lsp1109I GCAGC 2 cut(s) 210, 292
LweI GCATC 4 cut(s) 226, 387, 414, 487
MaeIII GTNAC 1 cut(s) 540
MalI GATC 3 cut(s) 96, 258, 272
MboI GATC 3 cut(s) 94, 256, 270
MboII GAAGA 9 cut(s) 5, 10, 19, 28, 76, 119, 122, 127, 280
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 2 cut(s) 80, 177
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 4 cut(s) 16, 212, 387, 431
MslI CAYNNNNRTG 1 cut(s) 224
MspI CCGG 1 cut(s) 444
Mva1269I GAATGC 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 207
NdeII GATC 3 cut(s) 94, 256, 270
NlaIII CATG 2 cut(s) 143, 340
NlaIV GGNNCC 2 cut(s) 217, 442
NmuCI GTSAC 1 cut(s) 540
PagI TCATGA 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 460
PctI GAATGC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 2 cut(s) 200, 307
PspN4I GGNNCC 2 cut(s) 217, 442
PspPI GGNCC 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 2 cut(s) 365, 527
RsaNI GTAC 2 cut(s) 364, 526
RseI CAYNNNNRTG 1 cut(s) 224
SatI GCNGC 2 cut(s) 199, 306
Sau3AI GATC 3 cut(s) 94, 256, 270
Sau96I GGNCC 1 cut(s) 188
SduI GDGCHC 1 cut(s) 286
SetI ASST 6 cut(s) 47, 126, 212, 224, 361, 551
SfaNI GCATC 4 cut(s) 226, 387, 414, 487
SmiMI CAYNNNNRTG 1 cut(s) 224
Sse9I AATT 2 cut(s) 80, 177
SsiI CCGC 1 cut(s) 186
TaaI ACNGT 1 cut(s) 487
TaqI TCGA 1 cut(s) 266
TasI AATT 2 cut(s) 80, 177
TatI WGTACW 2 cut(s) 363, 525
TfiI GAWTC 1 cut(s) 397
TscAI CASTG 4 cut(s) 250, 306, 330, 408
TseFI GTSAC 1 cut(s) 540
TseI GCWGC 2 cut(s) 198, 305
Tsp45I GTSAC 1 cut(s) 540
TspDTI ATGAA 1 cut(s) 422
TspRI CASTG 4 cut(s) 250, 306, 330, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.