MD07G1030400.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016911
Physical Location & Seq
Reverse (-)
2537379 .. 2537933
555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1030400.v1.1.491

Sequence Viewer

Length: 555 bp
ATGTCTTCGTTTCTCTACTACTCTATCTTCATCCTCTGCTCAACCTTTCTCTATTCCATTGATGGAGTTCTAATCAGGCAATTCGTCAGATCCGGCGGGAAAACAGAAAACTTAAGCCACCTCCACTTTTTCTTCCATGACATCCTTGGTGGCAAAAACCCAACCTCTGTTAGAATTATTGGGCCACCAAATGGTACCGCTGCAGGCTTTGGCAGCACTATGATGATCGATAATGCACTGACTGAAGAGCAAGACCGTGCCTCCAAGCTCGTCGGAAGAGCACAAGGGTTTTACTCAGTGGCTTCACAACAAGCAGATGAATTTGCACTCCTTATGGTCATGAATTTTGTTTTTGTGGAAGGCAAGTACAAGGGGAGTAGCATTAGCATTCTCGGTAGGAATCCGGTCTTGAACGATGTGAGAGAGATGCCAGTTGTTGGAGGCACTGGGCAGTTTAGGTCTGCTCGAGGTTATGCCTTAGCCCATACGGTTTGGTTTGATGCTAATACGGGAGATGCCATTGTTGAATACAATGTTTATGTGTCAGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

185

Amino Acids

20.19

Weight (kDa)

6.05

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 39 - 181 4.3e-58 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016911)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02330
malus_domestica MD07G1030400.v1.1
prunus_persica Prupe.2G028800_v2.0.a1 Prupe.2G028900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0319661
rosa_laevigata RLG00000030466
rosa_roxburghii Rroxscaffold_4G00328230
rosa_rugosa Rorug01G0029400
rosa_samantha Rh1AG041000 Rh1BG038200 Rh1CG042100 Rh1DG027800
rosa_wichuraiana Rw0G019960 Rw1G003410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 194
AccB7I CCANNNNNTGG 2 cut(s) 191, 437
AciI CCGC 2 cut(s) 96, 198
AclWI GGATC 1 cut(s) 84
AcsI RAATTY 2 cut(s) 320, 343
AcuI CTGAAG 1 cut(s) 264
AfaI GTAC 2 cut(s) 196, 368
AfiI CCNNNNNNNGG 2 cut(s) 191, 437
AflII CTTAAG 1 cut(s) 112
AgsI TTSAA 2 cut(s) 412, 527
AluBI AGCT 1 cut(s) 268
AluI AGCT 1 cut(s) 268
Alw21I GWGCWC 1 cut(s) 283
AlwI GGATC 1 cut(s) 84
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 200, 213
ApoI RAATTY 2 cut(s) 320, 343
Asp718I GGTACC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 465
BanI GGYRCC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 2 cut(s) 187, 225
BccI CCATC 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 201
BfrI CTTAAG 1 cut(s) 112
BisI GCNGC 2 cut(s) 201, 214
BlsI GCNGC 2 cut(s) 202, 215
BmeT110I CYCGRG 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 1 cut(s) 196
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 3 cut(s) 417, 490, 505
BmuI ACTGGG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 478
Bsa29I ATCGAT 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 145
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 437
Bse1I ACTGG 2 cut(s) 431, 451
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 2 cut(s) 30, 141
BseLI CCNNNNNNNGG 2 cut(s) 191, 437
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 431, 451
BseXI GCAGC 2 cut(s) 187, 225
BshFI GGCC 1 cut(s) 184
BshNI GGYRCC 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 283
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 2 cut(s) 93, 404
BslI CCNNNNNNNGG 2 cut(s) 191, 437
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 2 cut(s) 89, 225
BspACI CCGC 2 cut(s) 96, 198
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 1 cut(s) 308
BspDI ATCGAT 1 cut(s) 228
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 196
BspMAI CTGCAG 1 cut(s) 205
BspPI GGATC 1 cut(s) 84
BspQI GCTCTTC 2 cut(s) 240, 271
BspT107I GGYRCC 1 cut(s) 194
BspTI CTTAAG 1 cut(s) 112
BsrI ACTGG 2 cut(s) 431, 451
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 2 cut(s) 89, 225
BssT1I CCWWGG 1 cut(s) 145
Bst4CI ACNGT 2 cut(s) 257, 490
Bst6I CTCTTC 2 cut(s) 240, 271
BstAFI CTTAAG 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 2 cut(s) 295, 478
BstF5I GGATG 2 cut(s) 30, 141
BstKTI GATC 2 cut(s) 92, 228
BstMBI GATC 2 cut(s) 89, 225
BstMWI GCNNNNNNNGC 1 cut(s) 213
BstSFI CTRYAG 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 187, 225
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
Bsu15I ATCGAT 1 cut(s) 228
BsuRI GGCC 1 cut(s) 184
BsuTUI ATCGAT 1 cut(s) 228
BtsCI GGATG 2 cut(s) 30, 141
BtsIMutI CAGTG 3 cut(s) 236, 303, 444
Cac8I GCNNGC 1 cut(s) 205
CciI TCATGA 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 182
ClaI ATCGAT 1 cut(s) 228
Csp6I GTAC 2 cut(s) 195, 367
CviAII CATG 2 cut(s) 137, 340
CviJI RGCY 6 cut(s) 117, 184, 207, 268, 302, 482
CviKI_1 RGCY 6 cut(s) 117, 184, 207, 268, 302, 482
CviQI GTAC 2 cut(s) 195, 367
DdeI CTNAG 2 cut(s) 295, 478
DpnI GATC 2 cut(s) 91, 227
DpnII GATC 2 cut(s) 89, 225
Eam1104I CTCTTC 2 cut(s) 240, 271
EarI CTCTTC 2 cut(s) 240, 271
Eco130I CCWWGG 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 2 cut(s) 140, 343
FaiI YATR 7 cut(s) 138, 221, 335, 341, 474, 486, 540
FatI CATG 2 cut(s) 136, 339
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 2 cut(s) 201, 214
FokI GGATG 2 cut(s) 17, 128
Fsp4HI GCNGC 2 cut(s) 201, 214
GluI GCNGC 2 cut(s) 201, 214
HaeIII GGCC 1 cut(s) 184
HapII CCGG 2 cut(s) 93, 404
Hin1II CATG 2 cut(s) 140, 343
HinfI GANTC 2 cut(s) 400, 548
HpaII CCGG 2 cut(s) 93, 404
Hpy188I TCNGA 3 cut(s) 89, 275, 547
Hpy188III TCNNGA 3 cut(s) 340, 409, 552
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 353
HpyCH4III ACNGT 2 cut(s) 257, 490
HpyCH4V TGCA 3 cut(s) 203, 236, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 213
HpyF3I CTNAG 2 cut(s) 295, 478
Hsp92II CATG 2 cut(s) 140, 343
KpnI GGTACC 1 cut(s) 198
Kzo9I GATC 2 cut(s) 89, 225
LguI GCTCTTC 2 cut(s) 240, 271
LpnPI CCDG 6 cut(s) 61, 106, 189, 417, 432, 444
Lsp1109I GCAGC 2 cut(s) 187, 225
LweI GCATC 3 cut(s) 417, 490, 505
MalI GATC 2 cut(s) 91, 227
MboI GATC 2 cut(s) 89, 225
MboII GAAGA 4 cut(s) 19, 124, 257, 288
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 1 cut(s) 283
MluCI AATT 4 cut(s) 80, 174, 320, 343
MmeI TCCRAC 2 cut(s) 253, 418
MnlI CCTC 6 cut(s) 44, 131, 175, 271, 434, 461
MseI TTAA 1 cut(s) 113
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 200
MspCI CTTAAG 1 cut(s) 112
MspI CCGG 2 cut(s) 93, 404
Mva1269I GAATGC 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 213
NdeII GATC 2 cut(s) 89, 225
NlaIII CATG 2 cut(s) 140, 343
NlaIV GGNNCC 1 cut(s) 196
PaeR7I CTCGAG 1 cut(s) 465
PagI TCATGA 1 cut(s) 339
PciSI GCTCTTC 2 cut(s) 240, 271
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 2 cut(s) 400, 548
PflMI CCANNNNNTGG 2 cut(s) 191, 437
PkrI GCNGC 2 cut(s) 202, 215
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 1 cut(s) 182
PspXI VCTCGAGB 1 cut(s) 465
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 89
RsaI GTAC 2 cut(s) 196, 368
RsaNI GTAC 2 cut(s) 195, 367
RseI CAYNNNNRTG 1 cut(s) 221
SapI GCTCTTC 2 cut(s) 240, 271
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 2 cut(s) 201, 214
Sau3AI GATC 2 cut(s) 89, 225
Sau96I GGNCC 1 cut(s) 182
SduI GDGCHC 1 cut(s) 283
SetI ASST 6 cut(s) 47, 123, 167, 270, 461, 472
SfaNI GCATC 3 cut(s) 417, 490, 505
SfcI CTRYAG 1 cut(s) 201
Sfr274I CTCGAG 1 cut(s) 465
SlaI CTCGAG 1 cut(s) 465
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 112, 465
SmoI CTYRAG 2 cut(s) 112, 465
Sse9I AATT 4 cut(s) 80, 174, 320, 343
SsiI CCGC 2 cut(s) 96, 198
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 2 cut(s) 257, 490
TaqI TCGA 2 cut(s) 228, 466
TasI AATT 4 cut(s) 80, 174, 320, 343
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 2 cut(s) 400, 548
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 3 cut(s) 243, 303, 451
TseI GCWGC 2 cut(s) 200, 213
TspDTI ATGAA 3 cut(s) 19, 333, 356
TspRI CASTG 3 cut(s) 243, 303, 451
Van91I CCANNNNNTGG 2 cut(s) 191, 437
Vha464I CTTAAG 1 cut(s) 112
XapI RAATTY 2 cut(s) 320, 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XhoI CTCGAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.