FvH4_7g03700

exoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015680
Physical Location & Seq
Forward (+)
4220215 .. 4227489
7275 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03700.t1

Sequence Viewer

Length: 1248 bp
ATGATGGCCCTTGAACAGTCAGAAAATATGCAAAGGAACTGTGAGGCTTCCTTAAAATGCCTCCAAAGTAAGGGATTCCCTTACAACCTCCAATGTAATGGGAATCCCATAGAAGGATTTCCAGAGCATAAAGATGAACTTAGTAACCATCCAGGTGGGGGTAATTCTGAGTCGGACCGTCCTATGGGCAGCGAATTTCTGGAACCCCCAACTGAATGTCACAACAAACCTACCTACCATCATGATTTTGGCTACTGGTCAGCCTTTCATTTTGATTCTCAAAAGTTGCAGCAGTGCCAGATGAATTCTTTTGAGAGCCAATTTTATCAAAATCCTGTTGACAGTCGATTTCATTATGTTCCATTCAATGTGTTTGCTCAAAGCTACCCTAATGAGCTCCAGTTCCAAGATTTTCAGTATTTTGTGGTAATCGACTTTGAGGCTACATGTGATAAGGATAGAAATCCTCACCCTCAAGAGATAATTGAGTTTCCATCCGTGATAGTGAATAGCGTGACTGGCCAACTGGAAGCGTGTTTTCAGACTTATGTGCGGCCAACCTGCAATCATCTTCTGAGTGATTTCTGCAAGGATCTGACAGGCATACAGCAAATTCAGGTGGACAGAGGTGTCACTCTAAATGAGGCCCTTCTTAGGCATGATAAATGGCTTGAGAAGAAGGGGATAAAGAACACCAACTTTGCTGTTGTCACATGGTCAAACTGGGACTGTCGGGTCATGTTGGAATCTGAGTGTCGATTTAAGAAAATCCGAAAGCCTCCTTATTTTAACAGATGGATCAACTTGAAGGTGCCTTTCCGTGAGGTTTTTGGTGGCATGAGGTGCAACTTGAAGGAGGCAGTTCAAATTGCAGGCTTAGCATGGCAGGGACGGGCTCACTGTGGCCTGGATGATGCCAAAAACACCGCTCGCCTACTTTCTGTCCTCATGCGTAGGGGTTTCAAATTTGCCATAACAAACTCATTGTTGTGGCAGACTGCTGATCGCCCAGCAACACTGAAGCAGTCCCCAGAACACCTGTCACTGCCTCATCACCCTCTGAAACTGAAGGATATGAGCATTCCAGTATGCCAGTATCATCACCCATTCTGTTTCTGTGGGGTGAAGAGCAGCAGAGGAATGGTCCGGAAGCCCGGGCCAAAGCAAGGGAGCTTCTTCTTTGGCTGTGGGAACTGGACTGCCACCAGAGGCGCCCGCTGCCATTACTTTGAATGGGTATCCGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0071704 GO:0090304 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T zf-GRF

Protein Analysis

416

Amino Acids

47.92

Weight (kDa)

8.22

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 142 - 313 6.7e-25 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g03700
malus_domestica MD02G1278000.v1.1 MD07G1045900.v1.1
prunus_persica Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1
pyrus_communis pycom02g23800 pycom07g03310
rosa_chinensis RchiOBHm_Chr1g0326491
rosa_laevigata RLG00000030069
rosa_rugosa Rorug01G0061000
rosa_samantha Rh1AG076600 Rh1BG062500 Rh1CG075300 Rh1DG082000
rosa_wichuraiana Rw1G006120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 629, 734
Acc36I ACCTGC 1 cut(s) 569
AccB1I GGYRCC 2 cut(s) 811, 1211
AccBSI CCGCTC 1 cut(s) 929
AccII CGCG 1 cut(s) 1244
AccIII TCCGGA 1 cut(s) 1146
AciI CCGC 4 cut(s) 553, 927, 1216, 1242
AclWI GGATC 2 cut(s) 600, 806
AcoI YGGCCR 2 cut(s) 520, 554
AcsI RAATTY 4 cut(s) 194, 304, 612, 965
AcuI CTGAAG 2 cut(s) 1040, 1088
AcyI GRCGYC 1 cut(s) 1212
AfiI CCNNNNNNNGG 6 cut(s) 70, 113, 158, 184, 654, 1166
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 7 cut(s) 14, 367, 808, 853, 866, 964, 1232
AjnI CCWGG 2 cut(s) 151, 906
AluBI AGCT 3 cut(s) 384, 397, 1173
AluI AGCT 3 cut(s) 384, 397, 1173
Alw21I GWGCWC 1 cut(s) 399
AlwI GGATC 2 cut(s) 600, 806
Ama87I CYCGRG 1 cut(s) 1154
Aor13HI TCCGGA 1 cut(s) 1146
AoxI GGCC 6 cut(s) 6, 520, 554, 645, 904, 1157
ApeKI GCWGC 4 cut(s) 189, 289, 1131, 1218
ApoI RAATTY 4 cut(s) 194, 304, 612, 965
Asp700I GAANNNNTTC 1 cut(s) 117
AspLEI GCGC 1 cut(s) 1214
AspS9I GGNCC 5 cut(s) 7, 175, 646, 1144, 1157
AsuC2I CCSGG 2 cut(s) 1155, 1156
AsuHPI GGTGA 4 cut(s) 461, 1046, 1094, 1135
AvaI CYCGRG 1 cut(s) 1154
AvaII GGWCC 2 cut(s) 175, 1144
BalI TGGCCA 1 cut(s) 522
BanI GGYRCC 2 cut(s) 811, 1211
BanII GRGCYC 2 cut(s) 399, 898
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 4 cut(s) 201, 301, 1143, 1205
BccI CCATC 4 cut(s) 156, 246, 502, 789
BciT130I CCWGG 2 cut(s) 153, 908
BcnI CCSGG 2 cut(s) 1155, 1156
BfoI RGCGCY 1 cut(s) 1215
BfuAI ACCTGC 1 cut(s) 569
BisI GCNGC 5 cut(s) 190, 290, 554, 1132, 1219
BlpI GCTNAGC 1 cut(s) 877
BlsI GCNGC 5 cut(s) 191, 291, 555, 1133, 1220
Bme1390I CCNGG 4 cut(s) 153, 908, 1155, 1156
Bme18I GGWCC 2 cut(s) 175, 1144
BmeT110I CYCGRG 1 cut(s) 1154
BmgT120I GGNCC 5 cut(s) 7, 175, 646, 1144, 1157
BmiI GGNNCC 3 cut(s) 204, 813, 1213
BmrFI CCNGG 4 cut(s) 153, 908, 1155, 1156
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 1 cut(s) 733
BpmI CTGGAG 1 cut(s) 383
Bpu1102I GCTNAGC 1 cut(s) 877
BpuEI CTTGAG 2 cut(s) 459, 692
BpuMI CCSGG 2 cut(s) 1155, 1156
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 1212
BsaJI CCNNGG 1 cut(s) 1154
BsaWI WCCGGW 1 cut(s) 1146
Bsc4I CCNNNNNNNGG 6 cut(s) 70, 113, 158, 184, 654, 1166
Bse1I ACTGG 8 cut(s) 260, 400, 523, 531, 728, 1085, 1093, 1199
Bse8I GATNNNNATC 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 1146
BseBI CCWGG 2 cut(s) 153, 908
BseDI CCNNGG 1 cut(s) 1154
BseGI GGATG 3 cut(s) 148, 494, 916
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 6 cut(s) 70, 113, 158, 184, 654, 1166
BseMII CTCAG 3 cut(s) 159, 566, 741
BseNI ACTGG 8 cut(s) 260, 400, 523, 531, 728, 1085, 1093, 1199
BseXI GCAGC 4 cut(s) 201, 301, 1143, 1205
BseYI CCCAGC 1 cut(s) 1009
Bsh1236I CGCG 1 cut(s) 1244
BshFI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
BshNI GGYRCC 2 cut(s) 811, 1211
BsiHKAI GWGCWC 1 cut(s) 399
BsiHKCI CYCGRG 1 cut(s) 1154
BsiSI CCGG 2 cut(s) 1147, 1155
BslFI GGGAC 3 cut(s) 740, 903, 1012
BslI CCNNNNNNNGG 6 cut(s) 70, 113, 158, 184, 654, 1166
BsmFI GGGAC 3 cut(s) 740, 903, 1012
BsmI GAATGC 1 cut(s) 1080
BsnI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
BsoBI CYCGRG 1 cut(s) 1154
Bsp1286I GDGCHC 2 cut(s) 399, 898
Bsp13I TCCGGA 1 cut(s) 1146
Bsp143I GATC 3 cut(s) 592, 798, 1003
Bsp1720I GCTNAGC 1 cut(s) 877
BspACI CCGC 4 cut(s) 553, 927, 1216, 1242
BspANI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
BspCNI CTCAG 3 cut(s) 160, 567, 742
BspEI TCCGGA 1 cut(s) 1146
BspFNI CGCG 1 cut(s) 1244
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 204, 813, 1213
BspMI ACCTGC 1 cut(s) 569
BspPI GGATC 2 cut(s) 600, 806
BspQI GCTCTTC 1 cut(s) 1121
BspT107I GGYRCC 2 cut(s) 811, 1211
BsrBI CCGCTC 1 cut(s) 929
BsrI ACTGG 8 cut(s) 260, 400, 523, 531, 728, 1085, 1093, 1199
BssECI CCNNGG 1 cut(s) 1154
BssMI GATC 3 cut(s) 592, 798, 1003
BssNI GRCGYC 1 cut(s) 1212
Bst2UI CCWGG 2 cut(s) 153, 908
Bst4CI ACNGT 6 cut(s) 18, 41, 179, 344, 731, 902
Bst6I CTCTTC 1 cut(s) 1121
BstACI GRCGYC 1 cut(s) 1212
BstAPI GCANNNNNTGC 1 cut(s) 843
BstC8I GCNNGC 3 cut(s) 874, 931, 1216
BstDEI CTNAG 6 cut(s) 140, 168, 575, 653, 750, 877
BstF5I GGATG 3 cut(s) 148, 494, 916
BstFNI CGCG 1 cut(s) 1244
BstH2I RGCGCY 1 cut(s) 1215
BstHHI GCGC 1 cut(s) 1214
BstKTI GATC 3 cut(s) 595, 801, 1006
BstMBI GATC 3 cut(s) 592, 798, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 519, 843, 878, 1218
BstNI CCWGG 2 cut(s) 153, 908
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 4 cut(s) 151, 906, 1153, 1154
BstUI CGCG 1 cut(s) 1244
BstV1I GCAGC 4 cut(s) 201, 301, 1143, 1205
BstX2I RGATCY 1 cut(s) 592
BstXI CCANNNNNNTGG 2 cut(s) 98, 155
BstYI RGATCY 1 cut(s) 592
BsuRI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
BtsCI GGATG 3 cut(s) 148, 494, 916
BtsI GCAGTG 2 cut(s) 299, 1043
BtsIMutI CAGTG 4 cut(s) 299, 898, 1016, 1043
BveI ACCTGC 1 cut(s) 569
Cac8I GCNNGC 3 cut(s) 874, 931, 1216
CciI TCATGA 1 cut(s) 241
CfoI GCGC 1 cut(s) 1214
Cfr13I GGNCC 5 cut(s) 7, 175, 646, 1144, 1157
Cfr9I CCCGGG 1 cut(s) 1154
CpoI CGGWCCG 1 cut(s) 175
CspI CGGWCCG 1 cut(s) 175
CviAII CATG 8 cut(s) 242, 447, 659, 714, 739, 838, 882, 949
DdeI CTNAG 6 cut(s) 140, 168, 575, 653, 750, 877
DinI GGCGCC 1 cut(s) 1213
DpnI GATC 3 cut(s) 594, 800, 1005
DpnII GATC 3 cut(s) 592, 798, 1003
DrdI GACNNNNNNGTC 2 cut(s) 629, 734
DseDI GACNNNNNNGTC 2 cut(s) 629, 734
EaeI YGGCCR 2 cut(s) 520, 554
Eam1104I CTCTTC 1 cut(s) 1121
EarI CTCTTC 1 cut(s) 1121
Ecl136II GAGCTC 1 cut(s) 397
Eco24I GRGCYC 2 cut(s) 399, 898
Eco47I GGWCC 2 cut(s) 175, 1144
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 1040, 1088
Eco88I CYCGRG 1 cut(s) 1154
EcoICRI GAGCTC 1 cut(s) 397
EcoO109I RGGNCCY 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 151, 906
EcoT38I GRGCYC 2 cut(s) 399, 898
EgeI GGCGCC 1 cut(s) 1213
EheI GGCGCC 1 cut(s) 1213
FaeI CATG 8 cut(s) 245, 450, 662, 717, 742, 841, 885, 952
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 3 cut(s) 740, 903, 1012
FatI CATG 8 cut(s) 241, 446, 658, 713, 738, 837, 881, 948
FauI CCCGC 1 cut(s) 1223
Fnu4HI GCNGC 5 cut(s) 190, 290, 554, 1132, 1219
FokI GGATG 3 cut(s) 135, 481, 923
FriOI GRGCYC 2 cut(s) 399, 898
Fsp4HI GCNGC 5 cut(s) 190, 290, 554, 1132, 1219
GlaI GCGC 1 cut(s) 1213
GluI GCNGC 5 cut(s) 190, 290, 554, 1132, 1219
GsaI CCCAGC 1 cut(s) 1013
GsuI CTGGAG 1 cut(s) 383
HaeII RGCGCY 1 cut(s) 1215
HaeIII GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
HapII CCGG 2 cut(s) 1147, 1155
HhaI GCGC 1 cut(s) 1214
Hin1I GRCGYC 1 cut(s) 1212
Hin1II CATG 8 cut(s) 245, 450, 662, 717, 742, 841, 885, 952
Hin6I GCGC 1 cut(s) 1212
HinP1I GCGC 1 cut(s) 1212
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 5 cut(s) 75, 103, 170, 275, 746
HpaII CCGG 2 cut(s) 1147, 1155
HphI GGTGA 4 cut(s) 461, 1046, 1094, 1135
Hpy166II GTNNAC 2 cut(s) 340, 622
Hpy188I TCNGA 9 cut(s) 22, 169, 175, 543, 576, 597, 751, 773, 1062
Hpy188III TCNNGA 5 cut(s) 122, 200, 242, 476, 1147
Hpy8I GTNNAC 2 cut(s) 340, 622
HpyAV CCTTC 6 cut(s) 107, 659, 673, 802, 847, 1063
HpyCH4III ACNGT 6 cut(s) 18, 41, 179, 344, 731, 902
HpyCH4V TGCA 6 cut(s) 31, 289, 564, 588, 846, 872
HpyF10VI GCNNNNNNNGC 4 cut(s) 519, 843, 878, 1218
HpyF3I CTNAG 6 cut(s) 140, 168, 575, 653, 750, 877
Hsp92I GRCGYC 1 cut(s) 1212
Hsp92II CATG 8 cut(s) 245, 450, 662, 717, 742, 841, 885, 952
HspAI GCGC 1 cut(s) 1212
KasI GGCGCC 1 cut(s) 1211
Kpn2I TCCGGA 1 cut(s) 1146
Kzo9I GATC 3 cut(s) 592, 798, 1003
LguI GCTCTTC 1 cut(s) 1121
LmnI GCTCC 2 cut(s) 402, 1170
Lsp1109I GCAGC 4 cut(s) 201, 301, 1143, 1205
LweI GCATC 1 cut(s) 904
MaeIII GTNAC 6 cut(s) 143, 218, 514, 631, 709, 1041
MalI GATC 3 cut(s) 594, 800, 1005
MbiI CCGCTC 1 cut(s) 929
MboI GATC 3 cut(s) 592, 798, 1003
MboII GAAGA 4 cut(s) 563, 688, 1138, 1168
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 399, 898
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 8 cut(s) 163, 194, 304, 320, 483, 612, 867, 965
MluNI TGGCCA 1 cut(s) 522
Mly113I GGCGCC 1 cut(s) 1212
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 153, 723
Mox20I TGGCCA 1 cut(s) 522
MroI TCCGGA 1 cut(s) 1146
MroXI GAANNNNTTC 1 cut(s) 117
MscI TGGCCA 1 cut(s) 522
MseI TTAA 3 cut(s) 53, 762, 789
MslI CAYNNNNRTG 3 cut(s) 132, 153, 988
Msp20I TGGCCA 1 cut(s) 522
MspA1I CMGCKG 1 cut(s) 1218
MspI CCGG 2 cut(s) 1147, 1155
MspR9I CCNGG 4 cut(s) 153, 908, 1155, 1156
Mva1269I GAATGC 1 cut(s) 1080
MvaI CCWGG 2 cut(s) 153, 908
MvnI CGCG 1 cut(s) 1244
MwoI GCNNNNNNNGC 4 cut(s) 519, 843, 878, 1218
NarI GGCGCC 1 cut(s) 1212
NciI CCSGG 2 cut(s) 1155, 1156
NdeII GATC 3 cut(s) 592, 798, 1003
NlaIII CATG 8 cut(s) 245, 450, 662, 717, 742, 841, 885, 952
NlaIV GGNNCC 3 cut(s) 204, 813, 1213
NmuCI GTSAC 5 cut(s) 218, 514, 631, 709, 1041
NspI RCATGY 1 cut(s) 450
PagI TCATGA 1 cut(s) 241
PciI ACATGT 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 1121
PctI GAATGC 1 cut(s) 1080
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 4 cut(s) 75, 103, 275, 746
PkrI GCNGC 5 cut(s) 191, 291, 555, 1133, 1220
PleI GAGTC 1 cut(s) 178
PluTI GGCGCC 1 cut(s) 1215
PpsI GAGTC 1 cut(s) 178
PscI ACATGT 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 151, 906
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 2 cut(s) 151, 906
PspN4I GGNNCC 3 cut(s) 204, 813, 1213
PspPI GGNCC 5 cut(s) 7, 175, 646, 1144, 1157
PsuI RGATCY 1 cut(s) 592
RseI CAYNNNNRTG 3 cut(s) 132, 153, 988
Rsr2I CGGWCCG 1 cut(s) 175
RsrII CGGWCCG 1 cut(s) 175
SacI GAGCTC 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 1121
SaqAI TTAA 3 cut(s) 53, 762, 789
SatI GCNGC 5 cut(s) 190, 290, 554, 1132, 1219
Sau3AI GATC 3 cut(s) 592, 798, 1003
Sau96I GGNCC 5 cut(s) 7, 175, 646, 1144, 1157
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 4 cut(s) 153, 908, 1155, 1156
SduI GDGCHC 2 cut(s) 399, 898
SfaNI GCATC 1 cut(s) 904
SfoI GGCGCC 1 cut(s) 1213
SinI GGWCC 2 cut(s) 175, 1144
SmaI CCCGGG 1 cut(s) 1156
SmiMI CAYNNNNRTG 3 cut(s) 132, 153, 988
SmlI CTYRAG 2 cut(s) 474, 671
SmoI CTYRAG 2 cut(s) 474, 671
SrfI GCCCGGGC 1 cut(s) 1156
Sse9I AATT 8 cut(s) 163, 194, 304, 320, 483, 612, 867, 965
SsiI CCGC 4 cut(s) 553, 927, 1216, 1242
SspDI GGCGCC 1 cut(s) 1211
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 4 cut(s) 151, 906, 1153, 1154
TaaI ACNGT 6 cut(s) 18, 41, 179, 344, 731, 902
TaqI TCGA 3 cut(s) 346, 432, 757
TasI AATT 8 cut(s) 163, 194, 304, 320, 483, 612, 867, 965
TauI GCSGC 1 cut(s) 556
TfiI GAWTC 4 cut(s) 75, 103, 275, 746
Tru1I TTAA 3 cut(s) 53, 762, 789
Tru9I TTAA 3 cut(s) 53, 762, 789
TscAI CASTG 4 cut(s) 299, 905, 1023, 1050
TseFI GTSAC 5 cut(s) 218, 514, 631, 709, 1041
TseI GCWGC 4 cut(s) 189, 289, 1131, 1218
Tsp45I GTSAC 5 cut(s) 218, 514, 631, 709, 1041
TspDTI ATGAA 4 cut(s) 150, 257, 317, 341
TspGWI ACGGA 2 cut(s) 487, 809
TspMI CCCGGG 1 cut(s) 1154
TspRI CASTG 4 cut(s) 299, 905, 1023, 1050
VpaK11BI GGWCC 2 cut(s) 175, 1144
XapI RAATTY 4 cut(s) 194, 304, 612, 965
XceI RCATGY 1 cut(s) 450
XcmI CCANNNNNNNNNTGG 1 cut(s) 245
XmaI CCCGGG 1 cut(s) 1154
XmnI GAANNNNTTC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.