MD07G1045900.v1.1

exoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015680
Physical Location & Seq
Reverse (-)
3909084 .. 3912551
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1045900.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGTTGTTCTGCATACACTTTAAACTCGCCTACTCTTTGTTGTTTGATGTGTTGGTTTTTGCTGGTTATCCAATGACACGATACTTGTGTTTCATCTCATTTTATGTCTTTCCTGTTGCAGAAGATATGGAAAGGACCTGCGAGGCTTCCTTAAAATGCCTCCACAAGGGATTCCCATACAACCTCCAGTGTAATGGGAGTCCCATTGAAGGATTTCCAAATCTTAAAGATGCAATTAGTAATCATCCAGTTGGGGATGTTCCTGAATCAGACCCTCCATTGGGCAGCGAATTTCTTGAACCTCCAACTGAATGTCACAACAAACCTAACTACCATCATGATTTTGGATACTGGTCAACCTTCCATTATGACTCCCAAAAGGTGCAACAGTGCCAGATGAATGCTTTTGAGAGCCAGTTTTATCCCTTTCCCATGGAAAATCGATTTCATTATGCTCCATTCAATATGTTTGCTCAAAGCTACCCGAATGACTTCCAGTTCCAAGACTTTCAGTATTTTGTGGTAATAGACTTTGAGGCTACCTGCGATAAGGATAGAAATCCCCATCCTCAAGAGATAATCGAGTTTCCATCTGTGATAGTGAGTAGCATGACAGGCCAACTGGAAGCATGTTTTCAGACTTATGTGAGACCAACATGCAACCAAGTCTTAAGTGATTTCTGCAAGGACCTGACAGGAATCCAGCAAATTCAGGTGGATAGAGGAGTTACCTTAAGCGAGGCCCTTTTAAGACATGACAAATGGCTTGAGAAGAAGGGAATAAAAAACACCAACTTTGCTGTTGTGACATGGTCAAACTGGGACTGTCGGGTCATGTTGGAATCTGAGTGCCGATTCAAGAAAATTCGAAAGCCTCCGTATTTTAACCGATGGATCAACTTAAAGGTCCCTTTCCTTGAGGTTTTCGGTGGTGCTAGGTGCAACCTGAAGGAGGCTGTTCAAATGGCAGGCTTGGCATGGCAGGGCCGTGCTCACTGTGGCCTGGATGATGCCAAAAACACCGCTCGCCTACTTTCGCTTCTCATGTGCAGGGGCTTCAAATTTGCCATTACAAACTCATTGGTGTGGCAGACTGCTGACCGTCCTTTGACAATGAAACAATCCCCAGAGCACATGTCACCACCACATCACCCTCTGAAACTGAAGGAGATGGGTGCTCCAGTATTCCATTATCATCACCCATTCTGTTTCTGCGGGGTGAAGAGCAGCAAAGGTATGGTCCGGAAGCCAGGGCCAAAGCAAGGGAGCTTCTTCTTTGGCTGCGGAAACTGGACCGCCACTAGAGGCGCCCGTTGCCATTACTTCGAGTGGGCTTCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0071704 GO:0090304 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T zf-GRF

Protein Analysis

448

Amino Acids

51.61

Weight (kDa)

7.79

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 174 - 345 2.3e-24 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g03700
malus_domestica MD02G1278000.v1.1 MD07G1045900.v1.1
prunus_persica Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1
pyrus_communis pycom02g23800 pycom07g03310
rosa_chinensis RchiOBHm_Chr1g0326491
rosa_laevigata RLG00000030069
rosa_rugosa Rorug01G0061000
rosa_samantha Rh1AG076600 Rh1BG062500 Rh1CG075300 Rh1DG082000
rosa_wichuraiana Rw1G006120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 830
Acc36I ACCTGC 2 cut(s) 146, 551
AccB1I GGYRCC 1 cut(s) 1307
AccBSI CCGCTC 1 cut(s) 1025
AccIII TCCGGA 1 cut(s) 1242
AciI CCGC 4 cut(s) 1023, 1215, 1284, 1296
AclWI GGATC 1 cut(s) 902
AcsI RAATTY 4 cut(s) 290, 708, 864, 1061
AcuI CTGAAG 2 cut(s) 968, 1184
AcyI GRCGYC 1 cut(s) 1308
AfiI CCNNNNNNNGG 6 cut(s) 166, 209, 280, 281, 952, 1262
AflII CTTAAG 2 cut(s) 670, 733
AflIII ACRYGT 1 cut(s) 1134
AgsI TTSAA 6 cut(s) 209, 299, 463, 859, 962, 1060
AjnI CCWGG 2 cut(s) 1002, 1249
AluBI AGCT 2 cut(s) 480, 1269
AluI AGCT 2 cut(s) 480, 1269
Alw21I GWGCWC 3 cut(s) 994, 1134, 1180
Alw26I GTCTC 1 cut(s) 643
AlwI GGATC 1 cut(s) 902
Aor13HI TCCGGA 1 cut(s) 1242
AoxI GGCC 5 cut(s) 616, 741, 985, 1000, 1253
ApeKI GCWGC 3 cut(s) 285, 1227, 1281
ApoI RAATTY 4 cut(s) 290, 708, 864, 1061
ArsI GACNNNNNNTTYG 2 cut(s) 1224, 1256
Asp700I GAANNNNTTC 2 cut(s) 213, 491
AspLEI GCGC 1 cut(s) 1310
AspS9I GGNCC 8 cut(s) 135, 688, 742, 907, 985, 1240, 1253, 1293
AsuHPI GGTGA 4 cut(s) 1131, 1142, 1190, 1231
AsuII TTCGAA 1 cut(s) 868
AvaII GGWCC 5 cut(s) 135, 688, 907, 1240, 1293
BaeI ACNNNNGTAYC 2 cut(s) 73, 106
BanI GGYRCC 1 cut(s) 1307
Bbv12I GWGCWC 3 cut(s) 994, 1134, 1180
BbvI GCAGC 3 cut(s) 297, 1239, 1268
BccI CCATC 5 cut(s) 342, 573, 598, 885, 1165
BceAI ACGGC 1 cut(s) 972
BciT130I CCWGG 2 cut(s) 1004, 1251
BciVI GTATCC 1 cut(s) 341
BcoDI GTCTC 1 cut(s) 643
BfaI CTAG 2 cut(s) 936, 1302
BfoI RGCGCY 1 cut(s) 1311
BfrI CTTAAG 2 cut(s) 670, 733
BfuAI ACCTGC 2 cut(s) 146, 551
BfuI GTATCC 1 cut(s) 341
BisI GCNGC 3 cut(s) 286, 1228, 1282
BlsI GCNGC 3 cut(s) 287, 1229, 1283
Bme1390I CCNGG 2 cut(s) 1004, 1251
Bme18I GGWCC 5 cut(s) 135, 688, 907, 1240, 1293
BmgT120I GGNCC 8 cut(s) 135, 688, 742, 907, 985, 1240, 1253, 1293
BmiI GGNNCC 2 cut(s) 909, 1309
BmrFI CCNGG 2 cut(s) 1004, 1251
BmrI ACTGGG 1 cut(s) 829
BmsI GCATC 2 cut(s) 220, 1000
BmuI ACTGGG 1 cut(s) 829
BpmI CTGGAG 2 cut(s) 170, 1164
Bpu14I TTCGAA 1 cut(s) 868
BpuEI CTTGAG 3 cut(s) 555, 788, 938
Bsa29I ATCGAT 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 1308
BsaI GGTCTC 1 cut(s) 643
BsaJI CCNNGG 2 cut(s) 432, 1250
BsaWI WCCGGW 1 cut(s) 1242
Bsc4I CCNNNNNNNGG 6 cut(s) 166, 209, 280, 281, 952, 1262
Bse1I ACTGG 9 cut(s) 187, 248, 356, 415, 496, 627, 824, 1181, 1295
Bse8I GATNNNNATC 1 cut(s) 558
BseAI TCCGGA 1 cut(s) 1242
BseBI CCWGG 2 cut(s) 1004, 1251
BseCI ATCGAT 1 cut(s) 442
BseDI CCNNGG 2 cut(s) 432, 1250
BseGI GGATG 4 cut(s) 244, 262, 565, 1012
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 6 cut(s) 166, 209, 280, 281, 952, 1262
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 9 cut(s) 187, 248, 356, 415, 496, 627, 824, 1181, 1295
BseRI GAGGAG 1 cut(s) 738
BseXI GCAGC 3 cut(s) 297, 1239, 1268
BsgI GTGCAG 1 cut(s) 1069
BshFI GGCC 5 cut(s) 618, 743, 987, 1002, 1255
BshNI GGYRCC 1 cut(s) 1307
BshVI ATCGAT 1 cut(s) 442
BsiHKAI GWGCWC 3 cut(s) 994, 1134, 1180
BsiSI CCGG 1 cut(s) 1243
BslFI GGGAC 3 cut(s) 186, 836, 893
BslI CCNNNNNNNGG 6 cut(s) 166, 209, 280, 281, 952, 1262
BsmAI GTCTC 1 cut(s) 643
BsmFI GGGAC 3 cut(s) 186, 836, 893
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 5 cut(s) 618, 743, 987, 1002, 1255
Bso31I GGTCTC 1 cut(s) 643
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 3 cut(s) 994, 1134, 1180
Bsp13I TCCGGA 1 cut(s) 1242
Bsp143I GATC 1 cut(s) 894
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 4 cut(s) 1023, 1215, 1284, 1296
BspANI GGCC 5 cut(s) 618, 743, 987, 1002, 1255
BspCNI CTCAG 1 cut(s) 838
BspDI ATCGAT 1 cut(s) 442
BspEI TCCGGA 1 cut(s) 1242
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 909, 1309
BspMI ACCTGC 2 cut(s) 146, 551
BspPI GGATC 1 cut(s) 902
BspQI GCTCTTC 1 cut(s) 1217
BspT104I TTCGAA 1 cut(s) 868
BspT107I GGYRCC 1 cut(s) 1307
BspTI CTTAAG 2 cut(s) 670, 733
BspTNI GGTCTC 1 cut(s) 643
BsrBI CCGCTC 1 cut(s) 1025
BsrI ACTGG 9 cut(s) 187, 248, 356, 415, 496, 627, 824, 1181, 1295
BssECI CCNNGG 2 cut(s) 432, 1250
BssMI GATC 1 cut(s) 894
BssNI GRCGYC 1 cut(s) 1308
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 1004, 1251
Bst4CI ACNGT 4 cut(s) 390, 827, 998, 1103
Bst6I CTCTTC 1 cut(s) 1217
BstACI GRCGYC 1 cut(s) 1308
BstAFI CTTAAG 2 cut(s) 670, 733
BstBI TTCGAA 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 970, 1027
BstDEI CTNAG 1 cut(s) 846
BstDSI CCRYGG 1 cut(s) 432
BstENI CCTNNNNNAGG 2 cut(s) 164, 950
BstF5I GGATG 4 cut(s) 244, 262, 565, 1012
BstH2I RGCGCY 1 cut(s) 1311
BstHHI GCGC 1 cut(s) 1310
BstKTI GATC 1 cut(s) 897
BstMAI GTCTC 1 cut(s) 643
BstMBI GATC 1 cut(s) 894
BstMWI GCNNNNNNNGC 3 cut(s) 615, 974, 1314
BstNI CCWGG 2 cut(s) 1004, 1251
BstNSI RCATGY 3 cut(s) 633, 660, 1138
BstSCI CCNGG 2 cut(s) 1002, 1249
BstV1I GCAGC 3 cut(s) 297, 1239, 1268
BstXI CCANNNNNNTGG 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 442
BsuI GTATCC 1 cut(s) 341
BsuRI GGCC 5 cut(s) 618, 743, 987, 1002, 1255
BsuTUI ATCGAT 1 cut(s) 442
BtgI CCRYGG 1 cut(s) 432
BtsCI GGATG 4 cut(s) 244, 262, 565, 1012
BtsIMutI CAGTG 3 cut(s) 194, 395, 994
BveI ACCTGC 2 cut(s) 146, 551
Cac8I GCNNGC 2 cut(s) 970, 1027
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 1310
Cfr13I GGNCC 8 cut(s) 135, 688, 742, 907, 985, 1240, 1253, 1293
ClaI ATCGAT 1 cut(s) 442
DdeI CTNAG 1 cut(s) 846
DinI GGCGCC 1 cut(s) 1309
DpnI GATC 1 cut(s) 896
DpnII GATC 1 cut(s) 894
DraI TTTAAA 1 cut(s) 22
DrdI GACNNNNNNGTC 1 cut(s) 830
DseDI GACNNNNNNGTC 1 cut(s) 830
Eam1104I CTCTTC 1 cut(s) 1217
EarI CTCTTC 1 cut(s) 1217
Eco130I CCWWGG 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 643
Eco47I GGWCC 5 cut(s) 135, 688, 907, 1240, 1293
Eco57I CTGAAG 2 cut(s) 968, 1184
EcoNI CCTNNNNNAGG 2 cut(s) 164, 950
EcoO109I RGGNCCY 4 cut(s) 135, 688, 742, 907
EcoRII CCWGG 2 cut(s) 1002, 1249
EcoT14I CCWWGG 1 cut(s) 432
EgeI GGCGCC 1 cut(s) 1309
EheI GGCGCC 1 cut(s) 1309
ErhI CCWWGG 1 cut(s) 432
FaqI GGGAC 3 cut(s) 186, 836, 893
FauI CCCGC 1 cut(s) 1208
Fnu4HI GCNGC 3 cut(s) 286, 1228, 1282
FokI GGATG 4 cut(s) 231, 269, 552, 1019
Fsp4HI GCNGC 3 cut(s) 286, 1228, 1282
FspBI CTAG 2 cut(s) 936, 1302
GlaI GCGC 1 cut(s) 1309
GluI GCNGC 3 cut(s) 286, 1228, 1282
GsuI CTGGAG 2 cut(s) 170, 1164
HaeII RGCGCY 1 cut(s) 1311
HaeIII GGCC 5 cut(s) 618, 743, 987, 1002, 1255
HapII CCGG 1 cut(s) 1243
HhaI GCGC 1 cut(s) 1310
Hin1I GRCGYC 1 cut(s) 1308
Hin6I GCGC 1 cut(s) 1308
HinP1I GCGC 1 cut(s) 1308
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 7 cut(s) 171, 199, 266, 371, 699, 842, 855
HpaII CCGG 1 cut(s) 1243
HphI GGTGA 4 cut(s) 1131, 1142, 1190, 1231
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 4 cut(s) 271, 639, 847, 1158
Hpy188III TCNNGA 6 cut(s) 263, 296, 338, 572, 859, 1243
Hpy8I GTNNAC 1 cut(s) 357
HpyAV CCTTC 5 cut(s) 203, 370, 769, 943, 1159
HpyCH4III ACNGT 4 cut(s) 390, 827, 998, 1103
HpyCH4V TGCA 9 cut(s) 12, 119, 233, 385, 660, 684, 942, 1050, 1340
HpyF10VI GCNNNNNNNGC 3 cut(s) 615, 974, 1314
HpyF3I CTNAG 1 cut(s) 846
Hsp92I GRCGYC 1 cut(s) 1308
HspAI GCGC 1 cut(s) 1308
KasI GGCGCC 1 cut(s) 1307
Kpn2I TCCGGA 1 cut(s) 1242
Kzo9I GATC 1 cut(s) 894
LguI GCTCTTC 1 cut(s) 1217
LmnI GCTCC 3 cut(s) 460, 1183, 1266
Lsp1109I GCAGC 3 cut(s) 297, 1239, 1268
LweI GCATC 2 cut(s) 220, 1000
MaeI CTAG 2 cut(s) 936, 1302
MaeIII GTNAC 4 cut(s) 314, 727, 805, 1137
MalI GATC 1 cut(s) 896
MbiI CCGCTC 1 cut(s) 1025
MboI GATC 1 cut(s) 894
MboII GAAGA 4 cut(s) 134, 784, 1234, 1264
MhlI GDGCHC 3 cut(s) 994, 1134, 1180
MluCI AATT 5 cut(s) 234, 290, 708, 864, 1061
Mly113I GGCGCC 1 cut(s) 1308
MlyI GAGTC 2 cut(s) 208, 365
MmeI TCCRAC 2 cut(s) 329, 819
MroI TCCGGA 1 cut(s) 1242
MroXI GAANNNNTTC 2 cut(s) 213, 491
MseI TTAA 8 cut(s) 21, 152, 225, 671, 734, 749, 885, 902
MslI CAYNNNNRTG 1 cut(s) 1084
MspCI CTTAAG 2 cut(s) 670, 733
MspI CCGG 1 cut(s) 1243
MspR9I CCNGG 2 cut(s) 1004, 1251
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 2 cut(s) 1004, 1251
MwoI GCNNNNNNNGC 3 cut(s) 615, 974, 1314
NarI GGCGCC 1 cut(s) 1308
NcoI CCATGG 1 cut(s) 432
NdeII GATC 1 cut(s) 894
NlaIV GGNNCC 2 cut(s) 909, 1309
NmuCI GTSAC 3 cut(s) 314, 805, 1137
NspI RCATGY 3 cut(s) 633, 660, 1138
NspV TTCGAA 1 cut(s) 868
PagI TCATGA 1 cut(s) 337
PciI ACATGT 1 cut(s) 1134
PciSI GCTCTTC 1 cut(s) 1217
PctI GAATGC 1 cut(s) 406
PdmI GAANNNNTTC 2 cut(s) 213, 491
PfeI GAWTC 5 cut(s) 171, 266, 699, 842, 855
PflFI GACNNNGTC 1 cut(s) 811
PkrI GCNGC 3 cut(s) 287, 1229, 1283
PleI GAGTC 2 cut(s) 207, 365
PluTI GGCGCC 1 cut(s) 1311
PpsI GAGTC 2 cut(s) 207, 365
PpuMI RGGWCCY 3 cut(s) 135, 688, 907
PscI ACATGT 1 cut(s) 1134
Psp5II RGGWCCY 3 cut(s) 135, 688, 907
Psp6I CCWGG 2 cut(s) 1002, 1249
PspGI CCWGG 2 cut(s) 1002, 1249
PspN4I GGNNCC 2 cut(s) 909, 1309
PspPI GGNCC 8 cut(s) 135, 688, 742, 907, 985, 1240, 1253, 1293
PspPPI RGGWCCY 3 cut(s) 135, 688, 907
PsyI GACNNNGTC 1 cut(s) 811
RseI CAYNNNNRTG 1 cut(s) 1084
SapI GCTCTTC 1 cut(s) 1217
SaqAI TTAA 8 cut(s) 21, 152, 225, 671, 734, 749, 885, 902
SatI GCNGC 3 cut(s) 286, 1228, 1282
Sau3AI GATC 1 cut(s) 894
Sau96I GGNCC 8 cut(s) 135, 688, 742, 907, 985, 1240, 1253, 1293
SchI GAGTC 2 cut(s) 208, 365
ScrFI CCNGG 2 cut(s) 1004, 1251
SduI GDGCHC 3 cut(s) 994, 1134, 1180
SfaNI GCATC 2 cut(s) 220, 1000
SfoI GGCGCC 1 cut(s) 1309
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 5 cut(s) 135, 688, 907, 1240, 1293
SmiMI CAYNNNNRTG 1 cut(s) 1084
SmlI CTYRAG 5 cut(s) 570, 670, 733, 767, 917
SmoI CTYRAG 5 cut(s) 570, 670, 733, 767, 917
Sse9I AATT 5 cut(s) 234, 290, 708, 864, 1061
SsiI CCGC 4 cut(s) 1023, 1215, 1284, 1296
SspDI GGCGCC 1 cut(s) 1307
SspMI CTAG 2 cut(s) 936, 1302
StyD4I CCNGG 2 cut(s) 1002, 1249
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 390, 827, 998, 1103
TaqI TCGA 4 cut(s) 442, 582, 868, 1326
TasI AATT 5 cut(s) 234, 290, 708, 864, 1061
TfiI GAWTC 5 cut(s) 171, 266, 699, 842, 855
Tru1I TTAA 8 cut(s) 21, 152, 225, 671, 734, 749, 885, 902
Tru9I TTAA 8 cut(s) 21, 152, 225, 671, 734, 749, 885, 902
TscAI CASTG 3 cut(s) 194, 395, 1001
TseFI GTSAC 3 cut(s) 314, 805, 1137
TseI GCWGC 3 cut(s) 285, 1227, 1281
Tsp45I GTSAC 3 cut(s) 314, 805, 1137
TspDTI ATGAA 4 cut(s) 82, 413, 437, 1130
TspGWI ACGGA 1 cut(s) 867
TspRI CASTG 3 cut(s) 194, 395, 1001
Tth111I GACNNNGTC 1 cut(s) 811
Vha464I CTTAAG 2 cut(s) 670, 733
VpaK11BI GGWCC 5 cut(s) 135, 688, 907, 1240, 1293
XagI CCTNNNNNAGG 2 cut(s) 164, 950
XapI RAATTY 4 cut(s) 290, 708, 864, 1061
XceI RCATGY 3 cut(s) 633, 660, 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XmnI GAANNNNTTC 2 cut(s) 213, 491
XspI CTAG 2 cut(s) 936, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.