Prupe.2G042700_v2.0.a1

exoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0015680
Physical Location & Seq
Forward (+)
4701152 .. 4709816
8665 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G042700.1

Sequence Viewer

Length: 1248 bp
ATGATGGCCCTTGAACAAACAGAAAATATGCAAAGGAACTGCGAGGCTTCCTTAAAATGCCTCCACAACAAGGGATTCCCTTACAACCTCCAATGTAATGGGAATCCCATCGAAGGATTTGCAGAGCATAAAGATGATATTAGTACTCACCCAGGTGGGGATGTTGCTGAGCCAGACCGTCCTGTGGGCGGTGAATTTCTTGAACCTCCAACTGAATGTCACAACAAACCTACCTACCATCATGATTTTGGATACTGGTCAACCTTCCATTTTGACTCCCAAAAGGTGCAACAGTGCCAGATAAATGCTTTTGAGAGCCAGTTTCATCCCTTTCCTGTGGAAAATCGATTTAACTATGTCCCACTCAATATGTTTGCTCAAAGTTACCCAAATGAGTTCCAGTTCCAAGATTTTCAGTATTTTGTGGTAATAGACTTTGAGGCTACCTGCGATAAGGATAGAAATCCCCATCCTCAAGAGATAATCGAGTTTCCATCTGTGATAGTGAGTAGTGTGACAGGCCAACTGGAAGCATGTTTTCAGACTTATGTGCGGCCAACCTGCAACCAGCTCCTGAGTGATTTCTGCAAGGATCTGACAGGAATCCAGCAAATTCAGGTGGATAGAGGAGTTACCTTAAGCGAGGCCCTTCTTAGGCATGACAAATGGCTTGAGAAGAAGGGAATAAAGAACACCAACTTTGCTGTTGTGACATGGTCAAACTGGGACTGTCGGGTCATGTTGGAATCTGAGTGTCGATTCAAGAAAATTCGAAAGCCTCCTTATTTTAACAGATGGATCAACTTAAAGGTTCCTTTCCGGGAGGTTTTCGGTGGTGTTAGGTGCAACCTGAAGGAGGCAGTTCAAATGGCAGGCTTGGCATGGCAGGGCCGTGCTCACTGTGGCCTGGATGATGCGAAAAACACTGCTCGCCTACTCTCTCTGCTTATGCGTAGGGGCTTCAGATTCGCCATTACAAACTCTTTGGTGTGGCAGACTGCTGATCGCCCATTGACATTGAAGCAATCCTCAGAGCACCTGTCACTGCCACATCACCCCCTGAAACTGAAGGATATGAGTATTCCTCTGTTCCAGTATCATCACCCAATCTGTTTCTGTGGGGTGAAGAGCAGCAGAGGTATGGTTCGGAAGCCAGGTCCAAAGCAAGGGAGCTTCTTCTTTGGCTGTGGAAACTGGACCGCCACTAGAGGTGCCCGCTGCCATTACTTTGAATGGTCTTCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0071704 GO:0090304 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T zf-GRF

Protein Analysis

416

Amino Acids

47.92

Weight (kDa)

8.08

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g03700
malus_domestica MD02G1278000.v1.1 MD07G1045900.v1.1
prunus_persica Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1
pyrus_communis pycom02g23800 pycom07g03310
rosa_chinensis RchiOBHm_Chr1g0326491
rosa_laevigata RLG00000030069
rosa_rugosa Rorug01G0061000
rosa_samantha Rh1AG076600 Rh1BG062500 Rh1CG075300 Rh1DG082000
rosa_wichuraiana Rw1G006120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
Acc36I ACCTGC 2 cut(s) 455, 569
AccB1I GGYRCC 1 cut(s) 1211
AciI CCGC 4 cut(s) 189, 553, 1200, 1216
AclWI GGATC 2 cut(s) 600, 806
AcoI YGGCCR 1 cut(s) 554
AcsI RAATTY 3 cut(s) 194, 612, 768
AcuI CTGAAG 3 cut(s) 872, 946, 1088
AfaI GTAC 1 cut(s) 145
AfiI CCNNNNNNNGG 8 cut(s) 70, 113, 157, 184, 188, 654, 856, 1166
AflII CTTAAG 1 cut(s) 637
AgsI TTSAA 6 cut(s) 14, 203, 763, 866, 1021, 1232
AjnI CCWGG 3 cut(s) 151, 906, 1153
AleI CACNNNNGTG 1 cut(s) 153
AluBI AGCT 2 cut(s) 571, 1173
AluI AGCT 2 cut(s) 571, 1173
Alw21I GWGCWC 2 cut(s) 898, 1038
AlwI GGATC 2 cut(s) 600, 806
AlwNI CAGNNNCTG 1 cut(s) 574
AoxI GGCC 6 cut(s) 6, 520, 554, 645, 889, 904
ApeKI GCWGC 2 cut(s) 1131, 1218
ApoI RAATTY 3 cut(s) 194, 612, 768
AspS9I GGNCC 5 cut(s) 7, 646, 889, 1157, 1197
AsuC2I CCSGG 1 cut(s) 821
AsuHPI GGTGA 5 cut(s) 140, 203, 1046, 1094, 1135
AsuII TTCGAA 1 cut(s) 772
AvaII GGWCC 2 cut(s) 1157, 1197
BaeGI GKGCMC 1 cut(s) 1216
BanI GGYRCC 1 cut(s) 1211
BbsI GAAGAC 1 cut(s) 1230
Bbv12I GWGCWC 2 cut(s) 898, 1038
BbvI GCAGC 2 cut(s) 1143, 1205
BccI CCATC 5 cut(s) 116, 246, 477, 502, 789
BceAI ACGGC 1 cut(s) 876
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 3 cut(s) 153, 908, 1155
BciVI GTATCC 1 cut(s) 245
BcnI CCSGG 1 cut(s) 821
BfaI CTAG 1 cut(s) 1206
BfrI CTTAAG 1 cut(s) 637
BfuAI ACCTGC 2 cut(s) 455, 569
BfuI GTATCC 1 cut(s) 245
BisI GCNGC 3 cut(s) 554, 1132, 1219
BlpI GCTNAGC 1 cut(s) 168
BlsI GCNGC 3 cut(s) 555, 1133, 1220
BmcAI AGTACT 1 cut(s) 145
Bme1390I CCNGG 4 cut(s) 153, 821, 908, 1155
Bme18I GGWCC 2 cut(s) 1157, 1197
BmgT120I GGNCC 5 cut(s) 7, 646, 889, 1157, 1197
BmiI GGNNCC 2 cut(s) 813, 1213
BmrFI CCNGG 4 cut(s) 153, 821, 908, 1155
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 1 cut(s) 733
BpiI GAAGAC 1 cut(s) 1230
BplI GAGNNNNNCTC 2 cut(s) 1069, 1101
Bpu1102I GCTNAGC 1 cut(s) 168
Bpu14I TTCGAA 1 cut(s) 772
BpuEI CTTGAG 2 cut(s) 459, 692
BpuMI CCSGG 1 cut(s) 821
Bsa29I ATCGAT 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 8 cut(s) 70, 113, 157, 184, 188, 654, 856, 1166
Bse1I ACTGG 7 cut(s) 260, 319, 400, 531, 728, 1093, 1199
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 3 cut(s) 153, 908, 1155
BseCI ATCGAT 1 cut(s) 346
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 4 cut(s) 166, 325, 469, 916
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 8 cut(s) 70, 113, 157, 184, 188, 654, 856, 1166
BseMII CTCAG 4 cut(s) 159, 566, 741, 1044
BseNI ACTGG 7 cut(s) 260, 319, 400, 531, 728, 1093, 1199
BseRI GAGGAG 1 cut(s) 642
BseSI GKGCMC 1 cut(s) 1216
BseXI GCAGC 2 cut(s) 1143, 1205
BshFI GGCC 6 cut(s) 8, 522, 556, 647, 891, 906
BshNI GGYRCC 1 cut(s) 1211
BshVI ATCGAT 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 898, 1038
BsiSI CCGG 1 cut(s) 820
BslFI GGGAC 2 cut(s) 344, 740
BslI CCNNNNNNNGG 8 cut(s) 70, 113, 157, 184, 188, 654, 856, 1166
BsmFI GGGAC 2 cut(s) 344, 740
BsnI GGCC 6 cut(s) 8, 522, 556, 647, 891, 906
Bsp119I TTCGAA 1 cut(s) 772
Bsp1286I GDGCHC 3 cut(s) 898, 1038, 1216
Bsp143I GATC 3 cut(s) 592, 798, 1003
Bsp1720I GCTNAGC 1 cut(s) 168
BspACI CCGC 4 cut(s) 189, 553, 1200, 1216
BspANI GGCC 6 cut(s) 8, 522, 556, 647, 891, 906
BspCNI CTCAG 4 cut(s) 160, 567, 742, 1043
BspDI ATCGAT 1 cut(s) 346
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 813, 1213
BspMI ACCTGC 2 cut(s) 455, 569
BspPI GGATC 2 cut(s) 600, 806
BspQI GCTCTTC 1 cut(s) 1121
BspT104I TTCGAA 1 cut(s) 772
BspT107I GGYRCC 1 cut(s) 1211
BspTI CTTAAG 1 cut(s) 637
BsrI ACTGG 7 cut(s) 260, 319, 400, 531, 728, 1093, 1199
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 3 cut(s) 592, 798, 1003
Bst2UI CCWGG 3 cut(s) 153, 908, 1155
Bst4CI ACNGT 4 cut(s) 179, 294, 731, 902
Bst6I CTCTTC 1 cut(s) 1121
BstAFI CTTAAG 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 772
BstC8I GCNNGC 3 cut(s) 874, 931, 1216
BstDEI CTNAG 5 cut(s) 168, 575, 653, 750, 1030
BstENI CCTNNNNNAGG 1 cut(s) 854
BstF5I GGATG 4 cut(s) 166, 325, 469, 916
BstKTI GATC 3 cut(s) 595, 801, 1006
BstMBI GATC 3 cut(s) 592, 798, 1003
BstMWI GCNNNNNNNGC 1 cut(s) 878
BstNI CCWGG 3 cut(s) 153, 908, 1155
BstNSI RCATGY 1 cut(s) 537
BstSCI CCNGG 4 cut(s) 151, 819, 906, 1153
BstSLI GKGCMC 1 cut(s) 1216
BstV1I GCAGC 2 cut(s) 1143, 1205
BstV2I GAAGAC 1 cut(s) 1230
BstX2I RGATCY 1 cut(s) 592
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 1 cut(s) 592
Bsu15I ATCGAT 1 cut(s) 346
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 6 cut(s) 8, 522, 556, 647, 891, 906
BsuTUI ATCGAT 1 cut(s) 346
BtsCI GGATG 4 cut(s) 166, 325, 469, 916
BtsI GCAGTG 2 cut(s) 924, 1043
BtsIMutI CAGTG 4 cut(s) 299, 898, 924, 1043
BveI ACCTGC 2 cut(s) 455, 569
Cac8I GCNNGC 3 cut(s) 874, 931, 1216
CaiI CAGNNNCTG 1 cut(s) 574
CciI TCATGA 1 cut(s) 241
Cfr13I GGNCC 5 cut(s) 7, 646, 889, 1157, 1197
ClaI ATCGAT 1 cut(s) 346
Csp6I GTAC 1 cut(s) 144
CviAII CATG 7 cut(s) 242, 534, 659, 714, 739, 882, 1245
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 5 cut(s) 168, 575, 653, 750, 1030
DpnI GATC 3 cut(s) 594, 800, 1005
DpnII GATC 3 cut(s) 592, 798, 1003
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 554
Eam1104I CTCTTC 1 cut(s) 1121
EarI CTCTTC 1 cut(s) 1121
Eco47I GGWCC 2 cut(s) 1157, 1197
Eco57I CTGAAG 3 cut(s) 872, 946, 1088
EcoNI CCTNNNNNAGG 1 cut(s) 854
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 151, 906, 1153
FaeI CATG 7 cut(s) 245, 537, 662, 717, 742, 885, 1248
FaqI GGGAC 2 cut(s) 344, 740
FatI CATG 7 cut(s) 241, 533, 658, 713, 738, 881, 1244
FauI CCCGC 1 cut(s) 1223
Fnu4HI GCNGC 3 cut(s) 554, 1132, 1219
FokI GGATG 4 cut(s) 173, 312, 456, 923
Fsp4HI GCNGC 3 cut(s) 554, 1132, 1219
FspBI CTAG 1 cut(s) 1206
GluI GCNGC 3 cut(s) 554, 1132, 1219
HaeIII GGCC 6 cut(s) 8, 522, 556, 647, 891, 906
HapII CCGG 1 cut(s) 820
Hin1II CATG 7 cut(s) 245, 537, 662, 717, 742, 885, 1248
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 7 cut(s) 75, 103, 275, 603, 746, 759, 966
HpaII CCGG 1 cut(s) 820
HphI GGTGA 5 cut(s) 140, 203, 1046, 1094, 1135
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 6 cut(s) 543, 597, 751, 965, 1033, 1149
Hpy188III TCNNGA 5 cut(s) 200, 242, 476, 574, 763
Hpy8I GTNNAC 1 cut(s) 261
HpyAV CCTTC 6 cut(s) 107, 274, 659, 673, 847, 1063
HpyCH4III ACNGT 4 cut(s) 179, 294, 731, 902
HpyCH4V TGCA 6 cut(s) 31, 122, 289, 564, 588, 846
HpyF10VI GCNNNNNNNGC 1 cut(s) 878
HpyF3I CTNAG 5 cut(s) 168, 575, 653, 750, 1030
Hsp92II CATG 7 cut(s) 245, 537, 662, 717, 742, 885, 1248
Kzo9I GATC 3 cut(s) 592, 798, 1003
LguI GCTCTTC 1 cut(s) 1121
LmnI GCTCC 2 cut(s) 576, 1170
Lsp1109I GCAGC 2 cut(s) 1143, 1205
LweI GCATC 1 cut(s) 904
MaeI CTAG 1 cut(s) 1206
MaeIII GTNAC 6 cut(s) 218, 383, 514, 631, 709, 1041
MalI GATC 3 cut(s) 594, 800, 1005
MboI GATC 3 cut(s) 592, 798, 1003
MboII GAAGA 4 cut(s) 688, 1138, 1168, 1230
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 3 cut(s) 898, 1038, 1216
MluCI AATT 3 cut(s) 194, 612, 768
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 233, 723
MseI TTAA 5 cut(s) 53, 351, 638, 789, 806
MslI CAYNNNNRTG 2 cut(s) 132, 153
MspA1I CMGCKG 1 cut(s) 1218
MspCI CTTAAG 1 cut(s) 637
MspI CCGG 1 cut(s) 820
MspR9I CCNGG 4 cut(s) 153, 821, 908, 1155
MvaI CCWGG 3 cut(s) 153, 908, 1155
MwoI GCNNNNNNNGC 1 cut(s) 878
NciI CCSGG 1 cut(s) 821
NdeII GATC 3 cut(s) 592, 798, 1003
NlaIII CATG 7 cut(s) 245, 537, 662, 717, 742, 885, 1248
NlaIV GGNNCC 2 cut(s) 813, 1213
NmuCI GTSAC 4 cut(s) 218, 514, 709, 1041
NspI RCATGY 1 cut(s) 537
NspV TTCGAA 1 cut(s) 772
OliI CACNNNNGTG 1 cut(s) 153
PagI TCATGA 1 cut(s) 241
PciSI GCTCTTC 1 cut(s) 1121
PfeI GAWTC 6 cut(s) 75, 103, 603, 746, 759, 966
PflFI GACNNNGTC 1 cut(s) 715
PfoI TCCNGGA 1 cut(s) 819
PkrI GCNGC 3 cut(s) 555, 1133, 1220
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
Psp6I CCWGG 3 cut(s) 151, 906, 1153
PspGI CCWGG 3 cut(s) 151, 906, 1153
PspN4I GGNNCC 2 cut(s) 813, 1213
PspPI GGNCC 5 cut(s) 7, 646, 889, 1157, 1197
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 592
PsyI GACNNNGTC 1 cut(s) 715
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
RseI CAYNNNNRTG 2 cut(s) 132, 153
SapI GCTCTTC 1 cut(s) 1121
SaqAI TTAA 5 cut(s) 53, 351, 638, 789, 806
SatI GCNGC 3 cut(s) 554, 1132, 1219
Sau3AI GATC 3 cut(s) 592, 798, 1003
Sau96I GGNCC 5 cut(s) 7, 646, 889, 1157, 1197
ScaI AGTACT 1 cut(s) 145
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 4 cut(s) 153, 821, 908, 1155
SduI GDGCHC 3 cut(s) 898, 1038, 1216
SfaNI GCATC 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 772
SinI GGWCC 2 cut(s) 1157, 1197
SmiMI CAYNNNNRTG 2 cut(s) 132, 153
SmlI CTYRAG 3 cut(s) 474, 637, 671
SmoI CTYRAG 3 cut(s) 474, 637, 671
Sse9I AATT 3 cut(s) 194, 612, 768
SsiI CCGC 4 cut(s) 189, 553, 1200, 1216
SspMI CTAG 1 cut(s) 1206
StyD4I CCNGG 4 cut(s) 151, 819, 906, 1153
TaaI ACNGT 4 cut(s) 179, 294, 731, 902
TaqI TCGA 5 cut(s) 111, 346, 486, 757, 772
TasI AATT 3 cut(s) 194, 612, 768
TatI WGTACW 1 cut(s) 143
TauI GCSGC 1 cut(s) 556
TfiI GAWTC 6 cut(s) 75, 103, 603, 746, 759, 966
Tru1I TTAA 5 cut(s) 53, 351, 638, 789, 806
Tru9I TTAA 5 cut(s) 53, 351, 638, 789, 806
TscAI CASTG 4 cut(s) 299, 905, 931, 1050
TseFI GTSAC 4 cut(s) 218, 514, 709, 1041
TseI GCWGC 2 cut(s) 1131, 1218
Tsp45I GTSAC 4 cut(s) 218, 514, 709, 1041
TspDTI ATGAA 1 cut(s) 314
TspRI CASTG 4 cut(s) 299, 905, 931, 1050
Tth111I GACNNNGTC 1 cut(s) 715
Vha464I CTTAAG 1 cut(s) 637
VpaK11BI GGWCC 2 cut(s) 1157, 1197
XagI CCTNNNNNAGG 1 cut(s) 854
XapI RAATTY 3 cut(s) 194, 612, 768
XceI RCATGY 1 cut(s) 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 245
XspI CTAG 1 cut(s) 1206
ZrmI AGTACT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.