MD02G1278000.v1.1

exoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015680
Physical Location & Seq
Reverse (-)
33372668 .. 33379580
6913 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1278000.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGATGGCCCTTGAACAAACAGAAGATATGGAAAGGACCTGCGAGGCTTCCTTAAAATGCCTCCACAAGGGATTCCCGTACAACCTCAAGTGTAATGGGAATCCCATTGAAGGATTTCCAGAGCTTAAAGATGAAATTAGTAATCATCCAGCTGGGGATGTTCCTGAATCAAACCGTCCATTGGGCAGTGAATTTCTTGAACCTCCAACTGGATGTTACAACAAACCTACCTACCATCATGATTTTGGATACTGGTCAACTTTCCATAATGACTCTCAAAAGGTGCAACAGTGCCAGATGAATGCTTTTGAGAGCCAGTTTTATCCCTTTCCCATGGAAAATCGATTTCATTATGTTCCATTCAATATGTTTGCTCAAAGTTACCCAAATGACTTCCAGTTCCAAGACTTTCAGTATTTTGTGGTAATAGACTTTGAGGCTACCTGCGATAAGGATAGAAATCCCCATCCTCAAGAGATAATCGAGTTTCCATCTGTGATAGTGAGTAGCATGACAGGCCAACTGGAAGCATGTTTTCAGACTTATGTGAGACCAACATGCAACCAACTCTTGAGTGATTTCTGCAAGGATCTGACAGGAATCCAGCAAATTCAGGTGGATAGAGGAGTTACCTTAAGTGAGGCCCTCCTAAGGCATGACAAATGGCTTGAGAAGAAGGGAATAAAAAACACCAACTTTGCTGTTGTGACATGGTCAAACTGGGACTGTCGGGTCATGTTGGAATCCGAATGTCGATTCAAGAAAATTCGAAAGCCTCCATATTTTAACCGATGGATCAACTTAAAGGTCCCTTTCTGTGAGGTTTTCGGTGGTGCTAGGTGCAACTTGAAGGACGCAGTTCAAATGGCAGGCTTGGCATGGCAGGGCCGTCCTCACTGTGGCCTGGATGATGCCAAAAACACCGCTCGCCTACTTTCTCTTCTCATGCACAGGGGTTTCAAATTTGCAATCACAAACTCATTGGTCTGGCATACTGCTGACCGTCCTATGACAATGAAGCAATCCCCCGAGCACATGTCACCACCACACCACCCTCTGAAACTGAAGGAGATGGGTGTTCCCGTATTCCATTATCATCACCCATTCTGTTTCTGTGGGGTGAAGAGCAGCAGAGGTATGGTCCGCAAGCCAGGGCCAAAGCAAGGGAGCTTCTTCTTTGGCTGCGGAAACTGGACCGCCACCAGAGGCTCCCGTTGCCATTACTTCGAGTGGGCTTCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0071704 GO:0090304 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T zf-GRF

Protein Analysis

415

Amino Acids

47.88

Weight (kDa)

7.83

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 141 - 312 1.4e-24 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g03700
malus_domestica MD02G1278000.v1.1 MD07G1045900.v1.1
prunus_persica Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1
pyrus_communis pycom02g23800 pycom07g03310
rosa_chinensis RchiOBHm_Chr1g0326491
rosa_laevigata RLG00000030069
rosa_rugosa Rorug01G0061000
rosa_samantha Rh1AG076600 Rh1BG062500 Rh1CG075300 Rh1DG082000
rosa_wichuraiana Rw1G006120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 731
Acc36I ACCTGC 2 cut(s) 47, 452
AccBSI CCGCTC 1 cut(s) 926
AciI CCGC 4 cut(s) 924, 1144, 1185, 1197
AclWI GGATC 2 cut(s) 597, 803
AcsI RAATTY 4 cut(s) 191, 609, 765, 962
AcuI CTGAAG 1 cut(s) 1085
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 7 cut(s) 67, 110, 181, 209, 651, 899, 1163
AflII CTTAAG 1 cut(s) 634
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 8 cut(s) 14, 110, 200, 364, 760, 850, 863, 961
AjnI CCWGG 2 cut(s) 903, 1150
AluBI AGCT 3 cut(s) 124, 152, 1170
AluI AGCT 3 cut(s) 124, 152, 1170
Alw21I GWGCWC 1 cut(s) 1035
Alw26I GTCTC 1 cut(s) 544
AlwI GGATC 2 cut(s) 597, 803
Ama87I CYCGRG 1 cut(s) 1028
AoxI GGCC 6 cut(s) 6, 517, 642, 886, 901, 1154
ApeKI GCWGC 2 cut(s) 1128, 1182
ApoI RAATTY 4 cut(s) 191, 609, 765, 962
Asp700I GAANNNNTTC 1 cut(s) 114
AspS9I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
AsuHPI GGTGA 3 cut(s) 1032, 1091, 1132
AsuII TTCGAA 1 cut(s) 769
AvaI CYCGRG 1 cut(s) 1028
AvaII GGWCC 4 cut(s) 36, 808, 1141, 1194
AxyI CCTNAGG 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 1035
BbvI GCAGC 2 cut(s) 1140, 1169
BccI CCATC 5 cut(s) 243, 474, 499, 786, 1066
BceAI ACGGC 1 cut(s) 873
BciT130I CCWGG 2 cut(s) 905, 1152
BciVI GTATCC 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 1 cut(s) 837
BfrI CTTAAG 1 cut(s) 634
BfuAI ACCTGC 2 cut(s) 47, 452
BfuI GTATCC 1 cut(s) 242
BisI GCNGC 2 cut(s) 1129, 1183
BlsI GCNGC 2 cut(s) 1130, 1184
Bme1390I CCNGG 2 cut(s) 905, 1152
Bme18I GGWCC 4 cut(s) 36, 808, 1141, 1194
BmeT110I CYCGRG 1 cut(s) 1028
BmgT120I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
BmiI GGNNCC 2 cut(s) 810, 1210
BmrFI CCNGG 2 cut(s) 905, 1152
BmrI ACTGGG 1 cut(s) 730
BmsI GCATC 1 cut(s) 901
BmuI ACTGGG 1 cut(s) 730
Bpu14I TTCGAA 1 cut(s) 769
BpuEI CTTGAG 4 cut(s) 71, 456, 592, 689
Bsa29I ATCGAT 1 cut(s) 343
BsaBI GATNNNNATC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 333, 1151
Bsc4I CCNNNNNNNGG 7 cut(s) 67, 110, 181, 209, 651, 899, 1163
Bse1I ACTGG 7 cut(s) 214, 257, 316, 397, 528, 725, 1196
Bse21I CCTNAGG 1 cut(s) 650
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 2 cut(s) 905, 1152
BseCI ATCGAT 1 cut(s) 343
BseDI CCNNGG 2 cut(s) 333, 1151
BseGI GGATG 5 cut(s) 145, 163, 218, 466, 913
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 7 cut(s) 67, 110, 181, 209, 651, 899, 1163
BseNI ACTGG 7 cut(s) 214, 257, 316, 397, 528, 725, 1196
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 2 cut(s) 1140, 1169
BseYI CCCAGC 1 cut(s) 152
BshFI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
BshVI ATCGAT 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 1035
BsiHKCI CYCGRG 1 cut(s) 1028
BslFI GGGAC 2 cut(s) 737, 794
BslI CCNNNNNNNGG 7 cut(s) 67, 110, 181, 209, 651, 899, 1163
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 2 cut(s) 737, 794
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
Bso31I GGTCTC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 1028
Bsp119I TTCGAA 1 cut(s) 769
Bsp1286I GDGCHC 1 cut(s) 1035
Bsp143I GATC 2 cut(s) 589, 795
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 4 cut(s) 924, 1144, 1185, 1197
BspANI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
BspDI ATCGAT 1 cut(s) 343
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 2 cut(s) 810, 1210
BspMI ACCTGC 2 cut(s) 47, 452
BspPI GGATC 2 cut(s) 597, 803
BspQI GCTCTTC 1 cut(s) 1118
BspT104I TTCGAA 1 cut(s) 769
BspTI CTTAAG 1 cut(s) 634
BspTNI GGTCTC 1 cut(s) 544
BsrBI CCGCTC 1 cut(s) 926
BsrI ACTGG 7 cut(s) 214, 257, 316, 397, 528, 725, 1196
BssECI CCNNGG 2 cut(s) 333, 1151
BssMI GATC 2 cut(s) 589, 795
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 905, 1152
Bst4CI ACNGT 5 cut(s) 176, 291, 728, 899, 1004
Bst6I CTCTTC 2 cut(s) 945, 1118
BstAFI CTTAAG 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 769
BstC8I GCNNGC 3 cut(s) 871, 928, 1148
BstDEI CTNAG 1 cut(s) 650
BstDSI CCRYGG 1 cut(s) 333
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 5 cut(s) 145, 163, 218, 466, 913
BstKTI GATC 2 cut(s) 592, 798
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 2 cut(s) 589, 795
BstMWI GCNNNNNNNGC 3 cut(s) 516, 875, 1215
BstNI CCWGG 2 cut(s) 905, 1152
BstNSI RCATGY 3 cut(s) 534, 561, 1039
BstSCI CCNGG 2 cut(s) 903, 1150
BstV1I GCAGC 2 cut(s) 1140, 1169
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
Bsu15I ATCGAT 1 cut(s) 343
Bsu36I CCTNAGG 1 cut(s) 650
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
BsuTUI ATCGAT 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 5 cut(s) 145, 163, 218, 466, 913
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 3 cut(s) 193, 296, 895
BveI ACCTGC 2 cut(s) 47, 452
Cac8I GCNNGC 3 cut(s) 871, 928, 1148
CciI TCATGA 1 cut(s) 238
Cfr13I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
ClaI ATCGAT 1 cut(s) 343
CseI GACGC 1 cut(s) 863
Csp6I GTAC 1 cut(s) 79
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 650
DpnI GATC 2 cut(s) 591, 797
DpnII GATC 2 cut(s) 589, 795
DrdI GACNNNNNNGTC 1 cut(s) 731
DseDI GACNNNNNNGTC 1 cut(s) 731
Eam1104I CTCTTC 2 cut(s) 945, 1118
EarI CTCTTC 2 cut(s) 945, 1118
Eco130I CCWWGG 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 544
Eco47I GGWCC 4 cut(s) 36, 808, 1141, 1194
Eco57I CTGAAG 1 cut(s) 1085
Eco81I CCTNAGG 1 cut(s) 650
Eco88I CYCGRG 1 cut(s) 1028
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoO109I RGGNCCY 3 cut(s) 36, 643, 808
EcoRII CCWGG 2 cut(s) 903, 1150
EcoT14I CCWWGG 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 333
FaqI GGGAC 2 cut(s) 737, 794
Fnu4HI GCNGC 2 cut(s) 1129, 1183
FokI GGATG 5 cut(s) 132, 170, 225, 453, 920
Fsp4HI GCNGC 2 cut(s) 1129, 1183
FspBI CTAG 1 cut(s) 837
GluI GCNGC 2 cut(s) 1129, 1183
GsaI CCCAGC 1 cut(s) 156
HaeIII GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
HgaI GACGC 1 cut(s) 863
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 7 cut(s) 72, 100, 167, 272, 600, 743, 756
HphI GGTGA 3 cut(s) 1032, 1091, 1132
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 4 cut(s) 540, 594, 748, 1059
Hpy188III TCNNGA 7 cut(s) 119, 164, 197, 239, 473, 571, 760
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 4 cut(s) 104, 670, 844, 1060
HpyCH4III ACNGT 5 cut(s) 176, 291, 728, 899, 1004
HpyCH4V TGCA 7 cut(s) 286, 561, 585, 843, 949, 968, 1241
HpyF10VI GCNNNNNNNGC 3 cut(s) 516, 875, 1215
HpyF3I CTNAG 1 cut(s) 650
Kzo9I GATC 2 cut(s) 589, 795
LguI GCTCTTC 1 cut(s) 1118
LmnI GCTCC 2 cut(s) 1167, 1214
Lsp1109I GCAGC 2 cut(s) 1140, 1169
LweI GCATC 1 cut(s) 901
MaeI CTAG 1 cut(s) 837
MaeIII GTNAC 5 cut(s) 215, 380, 628, 706, 1038
MalI GATC 2 cut(s) 591, 797
MbiI CCGCTC 1 cut(s) 926
MboI GATC 2 cut(s) 589, 795
MboII GAAGA 5 cut(s) 35, 685, 932, 1135, 1165
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 1035
MluCI AATT 5 cut(s) 135, 191, 609, 765, 962
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 2 cut(s) 230, 720
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 5 cut(s) 53, 126, 635, 786, 803
MspA1I CMGCKG 1 cut(s) 152
MspCI CTTAAG 1 cut(s) 634
MspR9I CCNGG 2 cut(s) 905, 1152
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 2 cut(s) 905, 1152
MwoI GCNNNNNNNGC 3 cut(s) 516, 875, 1215
NcoI CCATGG 1 cut(s) 333
NdeII GATC 2 cut(s) 589, 795
NlaIV GGNNCC 2 cut(s) 810, 1210
NmuCI GTSAC 2 cut(s) 706, 1038
NspI RCATGY 3 cut(s) 534, 561, 1039
NspV TTCGAA 1 cut(s) 769
PagI TCATGA 1 cut(s) 238
PciI ACATGT 1 cut(s) 1035
PciSI GCTCTTC 1 cut(s) 1118
PctI GAATGC 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 6 cut(s) 72, 100, 167, 600, 743, 756
PflFI GACNNNGTC 1 cut(s) 712
PkrI GCNGC 2 cut(s) 1130, 1184
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PpuMI RGGWCCY 2 cut(s) 36, 808
PscI ACATGT 1 cut(s) 1035
Psp5II RGGWCCY 2 cut(s) 36, 808
Psp6I CCWGG 2 cut(s) 903, 1150
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 2 cut(s) 903, 1150
PspN4I GGNNCC 2 cut(s) 810, 1210
PspPI GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
PspPPI RGGWCCY 2 cut(s) 36, 808
PsuI RGATCY 1 cut(s) 589
PsyI GACNNNGTC 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 1118
SaqAI TTAA 5 cut(s) 53, 126, 635, 786, 803
SatI GCNGC 2 cut(s) 1129, 1183
Sau3AI GATC 2 cut(s) 589, 795
Sau96I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 2 cut(s) 905, 1152
SduI GDGCHC 1 cut(s) 1035
SfaNI GCATC 1 cut(s) 901
SfuI TTCGAA 1 cut(s) 769
SinI GGWCC 4 cut(s) 36, 808, 1141, 1194
SmlI CTYRAG 5 cut(s) 86, 471, 571, 634, 668
SmoI CTYRAG 5 cut(s) 86, 471, 571, 634, 668
Sse9I AATT 5 cut(s) 135, 191, 609, 765, 962
SsiI CCGC 4 cut(s) 924, 1144, 1185, 1197
SspMI CTAG 1 cut(s) 837
StyD4I CCNGG 2 cut(s) 903, 1150
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 5 cut(s) 176, 291, 728, 899, 1004
TaqI TCGA 5 cut(s) 343, 483, 754, 769, 1227
TasI AATT 5 cut(s) 135, 191, 609, 765, 962
TfiI GAWTC 6 cut(s) 72, 100, 167, 600, 743, 756
Tru1I TTAA 5 cut(s) 53, 126, 635, 786, 803
Tru9I TTAA 5 cut(s) 53, 126, 635, 786, 803
TscAI CASTG 3 cut(s) 193, 296, 902
TseFI GTSAC 2 cut(s) 706, 1038
TseI GCWGC 2 cut(s) 1128, 1182
Tsp45I GTSAC 2 cut(s) 706, 1038
TspDTI ATGAA 4 cut(s) 147, 314, 338, 1031
TspRI CASTG 3 cut(s) 193, 296, 902
Tth111I GACNNNGTC 1 cut(s) 712
Vha464I CTTAAG 1 cut(s) 634
VpaK11BI GGWCC 4 cut(s) 36, 808, 1141, 1194
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 4 cut(s) 191, 609, 765, 962
XceI RCATGY 3 cut(s) 534, 561, 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.