Rh1BG062500

exoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0015680
Physical Location & Seq
Forward (+)
9253251 .. 9261258
8008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG062500.1

Sequence Viewer

Length: 1248 bp
ATGATGGCCCTTGAACAAACAGAAAATATGCAAAGGAACTGTGAGGCTTCCTTAAAATGCCTCCACAGTAAGGGATTCCCTTACAACCTACAATGTAATGGAAATCCCATAGAAGGATTTCCAGAGCATAAAGATGAACTTAGTAACCATCCAGGTGGGGATAATTCTGAGTCCGACCGTCCATTAGGCAGCGAATTTCTGGAACCCCCAACTGAATGTCACAACAAACCTACCTACCATCATGATTTTGGGTACTGGTCAGCCTTTCATTTTGATTCTCAAAAGTTGCAACAGTGCCAGATGAATGCTTTTGAGAGCCAATTTTATCAGCTTCCTGTTGACAATCGATTTCATTATGTTCCATTCAATGTGTTTGCTCAAAGCTACCCGAACGAGTTCCAGTTCCAAGATTTTCAGTATTTTGTGGTAATAGACTTTGAGGCTACATGTGATAAGGATAGAAATCCCCATCCTCAAGAGATAATTGAGTTTCCATCTGTGATAGTGAGTAGCGTGACTGGCCAATTGGAAGCATGTTTTCAAACTTATGTGCGGCCGACCTGCAATCATCTGCTGAGTGATTTCTGCAAGGATCTGACAGGCATACAGCAAATTCAGGTGGACAGAGGAGTAACTCTGAGTGAGGCCCTTCTTAGGCATGATAAATGGCTTGAGAAGAAGGGGATAAAGAACACCAACTTTGCTGTTGTTACGTGGTCAAACTGGGACTGTCGGGTCATGTTGGAATCTGAGTGTCGATTCAAGAAAATTCGAAAGCCTCCTTATTTTAACCGATGGATCAACTTGAAGGTGCCTTTCCGTGAGGTTTTCGGTGGCATGAGGTGCAATCTGAAGGAGGCAGTTCAAATGGCAGGCTTAGCATGGCAGGGACGGGCTCACTGTGGCCTGGATGATGCCAAAAACACTGCTCGCCTACTTTCTGTCCTCATGCGTAGGGGTTTCAAATTTGCCATAACAAATTCATTGTTATGGCAGACTGCTGATCGTCCTGTAACATTGAAGCAGTCCCCAGAACACCTGTCACCGCCTCATCACACTCTGAAACTGAAGGATATGAGTATTCCTGTATGCCAGTATCATCACCCATTCTGTTTCTGTGGGGTGAAGAGCAGCAGAGGAATGGTCAGGAAGCCAGGGCCAAAACAGGGGAGTGTCTTCTTTGGCTGTGGGAACTGGACTGCCACTAGAGGTGCCCGCTGCCATTACTTTGAATGGGCGTCTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0071704 GO:0090304 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T zf-GRF

Protein Analysis

415

Amino Acids

47.93

Weight (kDa)

8.06

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_T PF00929 142 - 313 9.1e-25 Exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g03700
malus_domestica MD02G1278000.v1.1 MD07G1045900.v1.1
prunus_persica Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1
pyrus_communis pycom02g23800 pycom07g03310
rosa_chinensis RchiOBHm_Chr1g0326491
rosa_laevigata RLG00000030069
rosa_rugosa Rorug01G0061000
rosa_samantha Rh1AG076600 Rh1BG062500 Rh1CG075300 Rh1DG082000
rosa_wichuraiana Rw1G006120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
Acc36I ACCTGC 1 cut(s) 569
AccB1I GGYRCC 2 cut(s) 811, 1211
AciI CCGC 3 cut(s) 553, 1046, 1216
AclWI GGATC 2 cut(s) 600, 806
AcoI YGGCCR 2 cut(s) 520, 554
AcsI RAATTY 5 cut(s) 194, 612, 768, 965, 979
AcuI CTGAAG 2 cut(s) 872, 1088
AcyI GRCGYC 1 cut(s) 1238
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 4 cut(s) 70, 113, 654, 1166
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 9 cut(s) 14, 367, 542, 763, 808, 866, 964, 1021, 1232
AjnI CCWGG 3 cut(s) 151, 906, 1153
AluBI AGCT 2 cut(s) 331, 384
AluI AGCT 2 cut(s) 331, 384
AlwI GGATC 2 cut(s) 600, 806
AoxI GGCC 6 cut(s) 6, 520, 554, 645, 904, 1157
ApeKI GCWGC 3 cut(s) 189, 1131, 1218
ApoI RAATTY 5 cut(s) 194, 612, 768, 965, 979
Asp700I GAANNNNTTC 2 cut(s) 117, 395
AspS9I GGNCC 3 cut(s) 7, 646, 1157
AsuHPI GGTGA 3 cut(s) 1035, 1094, 1135
AsuII TTCGAA 1 cut(s) 772
BaeGI GKGCMC 1 cut(s) 1216
BalI TGGCCA 1 cut(s) 522
BanI GGYRCC 2 cut(s) 811, 1211
BanII GRGCYC 1 cut(s) 898
BbsI GAAGAC 1 cut(s) 1168
BbvI GCAGC 3 cut(s) 201, 1143, 1205
BccI CCATC 5 cut(s) 156, 246, 477, 502, 789
BciT130I CCWGG 3 cut(s) 153, 908, 1155
BfaI CTAG 1 cut(s) 1206
BfuAI ACCTGC 1 cut(s) 569
BisI GCNGC 4 cut(s) 190, 554, 1132, 1219
BlpI GCTNAGC 1 cut(s) 877
BlsI GCNGC 4 cut(s) 191, 555, 1133, 1220
Bme1390I CCNGG 3 cut(s) 153, 908, 1155
BmgT120I GGNCC 3 cut(s) 7, 646, 1157
BmiI GGNNCC 3 cut(s) 204, 813, 1213
BmrFI CCNGG 3 cut(s) 153, 908, 1155
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 1 cut(s) 733
BpiI GAAGAC 1 cut(s) 1168
Bpu1102I GCTNAGC 1 cut(s) 877
Bpu14I TTCGAA 1 cut(s) 772
BpuEI CTTGAG 2 cut(s) 459, 692
Bsa29I ATCGAT 1 cut(s) 346
BsaAI YACGTR 1 cut(s) 714
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 1238
BsaJI CCNNGG 1 cut(s) 1154
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 113, 654, 1166
Bse1I ACTGG 6 cut(s) 260, 400, 523, 728, 1093, 1199
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 3 cut(s) 153, 908, 1155
BseCI ATCGAT 1 cut(s) 346
BseDI CCNNGG 1 cut(s) 1154
BseGI GGATG 3 cut(s) 148, 469, 916
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 4 cut(s) 70, 113, 654, 1166
BseMII CTCAG 4 cut(s) 159, 566, 629, 741
BseNI ACTGG 6 cut(s) 260, 400, 523, 728, 1093, 1199
BseRI GAGGAG 1 cut(s) 642
BseSI GKGCMC 1 cut(s) 1216
BseX3I CGGCCG 1 cut(s) 554
BseXI GCAGC 3 cut(s) 201, 1143, 1205
Bsh1285I CGRYCG 2 cut(s) 178, 557
BshFI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
BshNI GGYRCC 2 cut(s) 811, 1211
BshVI ATCGAT 1 cut(s) 346
BsiEI CGRYCG 2 cut(s) 178, 557
BslFI GGGAC 3 cut(s) 740, 903, 1012
BslI CCNNNNNNNGG 4 cut(s) 70, 113, 654, 1166
BsmFI GGGAC 3 cut(s) 740, 903, 1012
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
Bsp119I TTCGAA 1 cut(s) 772
Bsp1286I GDGCHC 2 cut(s) 898, 1216
Bsp143I GATC 3 cut(s) 592, 798, 1003
Bsp1720I GCTNAGC 1 cut(s) 877
BspACI CCGC 3 cut(s) 553, 1046, 1216
BspANI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
BspCNI CTCAG 4 cut(s) 160, 567, 630, 742
BspDI ATCGAT 1 cut(s) 346
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 204, 813, 1213
BspMI ACCTGC 1 cut(s) 569
BspPI GGATC 2 cut(s) 600, 806
BspQI GCTCTTC 1 cut(s) 1121
BspT104I TTCGAA 1 cut(s) 772
BspT107I GGYRCC 2 cut(s) 811, 1211
BsrI ACTGG 6 cut(s) 260, 400, 523, 728, 1093, 1199
BssECI CCNNGG 1 cut(s) 1154
BssMI GATC 3 cut(s) 592, 798, 1003
BssNI GRCGYC 1 cut(s) 1238
Bst2UI CCWGG 3 cut(s) 153, 908, 1155
Bst4CI ACNGT 6 cut(s) 41, 68, 179, 294, 731, 902
Bst6I CTCTTC 1 cut(s) 1121
BstACI GRCGYC 1 cut(s) 1238
BstAPI GCANNNNNTGC 1 cut(s) 843
BstBAI YACGTR 1 cut(s) 714
BstBI TTCGAA 1 cut(s) 772
BstC8I GCNNGC 3 cut(s) 874, 931, 1216
BstDEI CTNAG 7 cut(s) 140, 168, 575, 638, 653, 750, 877
BstF5I GGATG 3 cut(s) 148, 469, 916
BstKTI GATC 3 cut(s) 595, 801, 1006
BstMBI GATC 3 cut(s) 592, 798, 1003
BstMCI CGRYCG 2 cut(s) 178, 557
BstMWI GCNNNNNNNGC 3 cut(s) 519, 843, 878
BstNI CCWGG 3 cut(s) 153, 908, 1155
BstNSI RCATGY 2 cut(s) 450, 537
BstSCI CCNGG 3 cut(s) 151, 906, 1153
BstSLI GKGCMC 1 cut(s) 1216
BstV1I GCAGC 3 cut(s) 201, 1143, 1205
BstV2I GAAGAC 1 cut(s) 1168
BstX2I RGATCY 1 cut(s) 592
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 592
BstZI CGGCCG 1 cut(s) 554
Bsu15I ATCGAT 1 cut(s) 346
BsuRI GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
BsuTUI ATCGAT 1 cut(s) 346
BtsCI GGATG 3 cut(s) 148, 469, 916
BtsI GCAGTG 1 cut(s) 924
BtsIMutI CAGTG 3 cut(s) 299, 898, 924
BveI ACCTGC 1 cut(s) 569
Cac8I GCNNGC 3 cut(s) 874, 931, 1216
CciI TCATGA 1 cut(s) 241
Cfr13I GGNCC 3 cut(s) 7, 646, 1157
ClaI ATCGAT 1 cut(s) 346
CseI GACGC 1 cut(s) 1227
Csp6I GTAC 1 cut(s) 253
CviAII CATG 8 cut(s) 242, 447, 534, 659, 739, 838, 882, 949
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 7 cut(s) 140, 168, 575, 638, 653, 750, 877
DpnI GATC 3 cut(s) 594, 800, 1005
DpnII GATC 3 cut(s) 592, 798, 1003
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 2 cut(s) 520, 554
EagI CGGCCG 1 cut(s) 554
Eam1104I CTCTTC 1 cut(s) 1121
EarI CTCTTC 1 cut(s) 1121
EclXI CGGCCG 1 cut(s) 554
Eco24I GRGCYC 1 cut(s) 898
Eco52I CGGCCG 1 cut(s) 554
Eco57I CTGAAG 2 cut(s) 872, 1088
EcoO109I RGGNCCY 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 151, 906, 1153
EcoT38I GRGCYC 1 cut(s) 898
FaeI CATG 8 cut(s) 245, 450, 537, 662, 742, 841, 885, 952
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 3 cut(s) 740, 903, 1012
FatI CATG 8 cut(s) 241, 446, 533, 658, 738, 837, 881, 948
FauI CCCGC 1 cut(s) 1223
Fnu4HI GCNGC 4 cut(s) 190, 554, 1132, 1219
FokI GGATG 3 cut(s) 135, 456, 923
FriOI GRGCYC 1 cut(s) 898
Fsp4HI GCNGC 4 cut(s) 190, 554, 1132, 1219
FspBI CTAG 1 cut(s) 1206
GluI GCNGC 4 cut(s) 190, 554, 1132, 1219
HaeIII GGCC 6 cut(s) 8, 522, 556, 647, 906, 1159
HgaI GACGC 1 cut(s) 1227
Hin1I GRCGYC 1 cut(s) 1238
Hin1II CATG 8 cut(s) 245, 450, 537, 662, 742, 841, 885, 952
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 5 cut(s) 75, 170, 275, 746, 759
HphI GGTGA 3 cut(s) 1035, 1094, 1135
Hpy166II GTNNAC 2 cut(s) 340, 622
Hpy188I TCNGA 7 cut(s) 169, 175, 597, 639, 751, 852, 1062
Hpy188III TCNNGA 6 cut(s) 122, 200, 242, 476, 763, 1147
Hpy8I GTNNAC 2 cut(s) 340, 622
HpyAV CCTTC 6 cut(s) 107, 659, 673, 802, 847, 1063
HpyCH4III ACNGT 6 cut(s) 41, 68, 179, 294, 731, 902
HpyCH4IV ACGT 1 cut(s) 713
HpyCH4V TGCA 5 cut(s) 31, 289, 564, 588, 846
HpyF10VI GCNNNNNNNGC 3 cut(s) 519, 843, 878
HpyF3I CTNAG 7 cut(s) 140, 168, 575, 638, 653, 750, 877
HpySE526I ACGT 1 cut(s) 713
Hsp92I GRCGYC 1 cut(s) 1238
Hsp92II CATG 8 cut(s) 245, 450, 537, 662, 742, 841, 885, 952
Kzo9I GATC 3 cut(s) 592, 798, 1003
LguI GCTCTTC 1 cut(s) 1121
Lsp1109I GCAGC 3 cut(s) 201, 1143, 1205
LweI GCATC 1 cut(s) 904
MaeI CTAG 1 cut(s) 1206
MaeII ACGT 1 cut(s) 713
MaeIII GTNAC 7 cut(s) 143, 218, 514, 631, 709, 1012, 1041
MalI GATC 3 cut(s) 594, 800, 1005
MboI GATC 3 cut(s) 592, 798, 1003
MboII GAAGA 3 cut(s) 688, 1138, 1168
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 898, 1216
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 9 cut(s) 163, 194, 320, 483, 524, 612, 768, 965, 979
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 198, 723
Mox20I TGGCCA 1 cut(s) 522
MroXI GAANNNNTTC 2 cut(s) 117, 395
MscI TGGCCA 1 cut(s) 522
MseI TTAA 2 cut(s) 53, 789
MslI CAYNNNNRTG 3 cut(s) 132, 153, 988
Msp20I TGGCCA 1 cut(s) 522
MspA1I CMGCKG 1 cut(s) 1218
MspR9I CCNGG 3 cut(s) 153, 908, 1155
MunI CAATTG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 3 cut(s) 153, 908, 1155
MwoI GCNNNNNNNGC 3 cut(s) 519, 843, 878
NdeII GATC 3 cut(s) 592, 798, 1003
NlaIII CATG 8 cut(s) 245, 450, 537, 662, 742, 841, 885, 952
NlaIV GGNNCC 3 cut(s) 204, 813, 1213
NmuCI GTSAC 3 cut(s) 218, 514, 1041
NspI RCATGY 2 cut(s) 450, 537
NspV TTCGAA 1 cut(s) 772
PagI TCATGA 1 cut(s) 241
PciI ACATGT 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 1121
PctI GAATGC 1 cut(s) 310
PdmI GAANNNNTTC 2 cut(s) 117, 395
PfeI GAWTC 4 cut(s) 75, 275, 746, 759
PkrI GCNGC 4 cut(s) 191, 555, 1133, 1220
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Ppu21I YACGTR 1 cut(s) 714
PscI ACATGT 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 151, 906, 1153
PspGI CCWGG 3 cut(s) 151, 906, 1153
PspN4I GGNNCC 3 cut(s) 204, 813, 1213
PspPI GGNCC 3 cut(s) 7, 646, 1157
PsuI RGATCY 1 cut(s) 592
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 3 cut(s) 132, 153, 988
SapI GCTCTTC 1 cut(s) 1121
SaqAI TTAA 2 cut(s) 53, 789
SatI GCNGC 4 cut(s) 190, 554, 1132, 1219
Sau3AI GATC 3 cut(s) 592, 798, 1003
Sau96I GGNCC 3 cut(s) 7, 646, 1157
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 3 cut(s) 153, 908, 1155
SduI GDGCHC 2 cut(s) 898, 1216
SfaNI GCATC 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 772
SmiMI CAYNNNNRTG 3 cut(s) 132, 153, 988
SmlI CTYRAG 2 cut(s) 474, 671
SmoI CTYRAG 2 cut(s) 474, 671
Sse9I AATT 9 cut(s) 163, 194, 320, 483, 524, 612, 768, 965, 979
SsiI CCGC 3 cut(s) 553, 1046, 1216
SspMI CTAG 1 cut(s) 1206
StyD4I CCNGG 3 cut(s) 151, 906, 1153
TaaI ACNGT 6 cut(s) 41, 68, 179, 294, 731, 902
TaiI ACGT 1 cut(s) 716
TaqI TCGA 3 cut(s) 346, 757, 772
TasI AATT 9 cut(s) 163, 194, 320, 483, 524, 612, 768, 965, 979
TauI GCSGC 1 cut(s) 556
TfiI GAWTC 4 cut(s) 75, 275, 746, 759
Tru1I TTAA 2 cut(s) 53, 789
Tru9I TTAA 2 cut(s) 53, 789
TscAI CASTG 3 cut(s) 299, 905, 931
TseFI GTSAC 3 cut(s) 218, 514, 1041
TseI GCWGC 3 cut(s) 189, 1131, 1218
Tsp45I GTSAC 3 cut(s) 218, 514, 1041
TspDTI ATGAA 5 cut(s) 150, 257, 317, 341, 972
TspGWI ACGGA 1 cut(s) 809
TspRI CASTG 3 cut(s) 299, 905, 931
XapI RAATTY 5 cut(s) 194, 612, 768, 965, 979
XceI RCATGY 2 cut(s) 450, 537
XcmI CCANNNNNNNNNTGG 1 cut(s) 245
XmnI GAANNNNTTC 2 cut(s) 117, 395
XspI CTAG 1 cut(s) 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.