pycom07g03310

exoribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015680
Physical Location & Seq
Reverse (-)
2745823 .. 2751510
5688 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03310.4

Sequence Viewer

Length: 1245 bp
ATGATGGCCCTTGAACAACCCGAACATATGGAAAGGACCTGCGAGGCTTCCTTAAAATGCCTCCACAAGGGATTCCCATACAACCTCCAGTGTAATGGGAGTCCCATTGAAGGATTTCCAAATCTTAAAGATGCAATTAGTAATCATCCAGTTGGGGATGTTCCTGAATCAGACCGTCCATTGGGCAGCGAATTTCTTGAACCTCCAACTGAATGTCACGACAAACCTAACTACCATCATGATTTTGGATACTGGTCAACCTTCCATTATGACTCCCAAAAGGTGCAACAGTGCCAGACGAATGCTTTTGAGAGCCAGTTTTATCCCTTTCCCATGGAAAATCGATTTCATTATGCTCCATTCAATATGTTTGCTCAAAGTTACCCGAATGACTTCCAGTTCCAAGACTTTCAGTATTTTGTGGTAATAGACTTTGAGGCTACCTGCGATAAGGATAGAAATCCCCATCCTCAAGAGATAATCGAGTTTCCATCTGTGATAGTGAGTAGCATGACAGGCCAACTGGAAGCATGTTTTCAGACTTACGTGAGACCAACATGCAACCAACTCTTAAGTGATTTCTGCAAGGATCTTACAGGAATCCAGCAAATTCAGGTGGATAGAGGAGTTACCATAAGTGAGGCCCTTTTAAGGCATGACAAATGGCTTGAGAAGAAGGGAATAAAAAACACCAACTTTGCTGTTGTGACATGGTCAAACTGGGACTGTCGGGTCATGTTGGAATCTGAGTGCCGATTCAAGAAAATTCGAAAGCCTCCATATTTTAACCGGTGGATCAACTTAAAGGTCCCTTTCCGTGAGGTTTTTGGTGGTGCTAGGTGCAACCTGAAGGAGGCAGTTCAAATGGCAGGGTTGGCATGGCAGGGCCGTGCTCACTGTGGCCTGGATGATGCCAAAAACACCGCTCGCCTACTTTCGCTTCTCATGTGCAGGGGCTTCAAATTTGCCATTACAAACTCATTGGTGTGGCAGACTGCTGACCGTCCCTTGACAATGAAACAATCCCCAGACCACATGTCACCACCACATCACCCTCTGAAACTGAAGGAGATGGGCGCTCCAGTATTCCATTATCATCACCCATTCTGTTTCTGTGGGGTGAAGAGCAGCAAAGGTATGGTCCGGAAGCCAGGGCCAAAGCAAGGGAGCTTCTTCTTTGGCTGTGGAAACTGGACCGCCACTAGAGGCGCCCGTTGCCATTACTTCGAGTGGGCTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0071704 GO:0090304 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNase_T zf-GRF

Protein Analysis

415

Amino Acids

47.77

Weight (kDa)

7.83

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g03700
malus_domestica MD02G1278000.v1.1 MD07G1045900.v1.1
prunus_persica Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1 Prupe.2G042700_v2.0.a1
pyrus_communis pycom02g23800 pycom07g03310
rosa_chinensis RchiOBHm_Chr1g0326491
rosa_laevigata RLG00000030069
rosa_rugosa Rorug01G0061000
rosa_samantha Rh1AG076600 Rh1BG062500 Rh1CG075300 Rh1DG082000
rosa_wichuraiana Rw1G006120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 731
Acc36I ACCTGC 2 cut(s) 47, 452
AccB1I GGYRCC 1 cut(s) 1208
AccBSI CCGCTC 1 cut(s) 926
AccIII TCCGGA 1 cut(s) 1143
AciI CCGC 2 cut(s) 924, 1197
AclWI GGATC 2 cut(s) 597, 803
AcsI RAATTY 4 cut(s) 191, 609, 765, 962
AcuI CTGAAG 2 cut(s) 869, 1085
AcyI GRCGYC 1 cut(s) 1209
AfiI CCNNNNNNNGG 6 cut(s) 67, 110, 181, 651, 853, 1163
AflII CTTAAG 1 cut(s) 571
AflIII ACRYGT 1 cut(s) 1035
AgeI ACCGGT 1 cut(s) 789
AgsI TTSAA 7 cut(s) 14, 110, 200, 364, 760, 863, 961
AhdI GACNNNNNGTC 1 cut(s) 1036
AjnI CCWGG 2 cut(s) 903, 1150
AluBI AGCT 1 cut(s) 1170
AluI AGCT 1 cut(s) 1170
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 1 cut(s) 544
AlwI GGATC 2 cut(s) 597, 803
Aor13HI TCCGGA 1 cut(s) 1143
AoxI GGCC 6 cut(s) 6, 517, 642, 886, 901, 1154
ApeKI GCWGC 2 cut(s) 186, 1128
ApoI RAATTY 4 cut(s) 191, 609, 765, 962
ArsI GACNNNNNNTTYG 2 cut(s) 1125, 1157
AsiGI ACCGGT 1 cut(s) 789
Asp700I GAANNNNTTC 2 cut(s) 114, 392
AspLEI GCGC 2 cut(s) 1079, 1211
AspS9I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
AsuHPI GGTGA 4 cut(s) 1032, 1043, 1091, 1132
AsuII TTCGAA 1 cut(s) 769
AvaII GGWCC 4 cut(s) 36, 808, 1141, 1194
BanI GGYRCC 1 cut(s) 1208
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 2 cut(s) 198, 1140
BccI CCATC 4 cut(s) 243, 474, 499, 1066
BceAI ACGGC 1 cut(s) 873
BciT130I CCWGG 2 cut(s) 905, 1152
BciVI GTATCC 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 2 cut(s) 837, 1203
BfoI RGCGCY 2 cut(s) 1080, 1212
BfrI CTTAAG 1 cut(s) 571
BfuAI ACCTGC 2 cut(s) 47, 452
BfuI GTATCC 1 cut(s) 242
BisI GCNGC 2 cut(s) 187, 1129
BlsI GCNGC 2 cut(s) 188, 1130
Bme1390I CCNGG 2 cut(s) 905, 1152
Bme18I GGWCC 4 cut(s) 36, 808, 1141, 1194
BmeRI GACNNNNNGTC 1 cut(s) 1036
BmgT120I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
BmiI GGNNCC 2 cut(s) 810, 1210
BmrFI CCNGG 2 cut(s) 905, 1152
BmrI ACTGGG 1 cut(s) 730
BmsI GCATC 2 cut(s) 121, 901
BmuI ACTGGG 1 cut(s) 730
BpmI CTGGAG 2 cut(s) 71, 1065
Bpu14I TTCGAA 1 cut(s) 769
BpuEI CTTGAG 2 cut(s) 456, 689
Bsa29I ATCGAT 1 cut(s) 343
BsaAI YACGTR 1 cut(s) 547
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 1209
BsaI GGTCTC 1 cut(s) 544
BsaJI CCNNGG 2 cut(s) 333, 1151
BsaWI WCCGGW 2 cut(s) 789, 1143
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 110, 181, 651, 853, 1163
Bse118I RCCGGY 1 cut(s) 789
Bse1I ACTGG 9 cut(s) 88, 149, 257, 316, 397, 528, 725, 1082, 1196
Bse8I GATNNNNATC 1 cut(s) 459
BseAI TCCGGA 1 cut(s) 1143
BseBI CCWGG 2 cut(s) 905, 1152
BseCI ATCGAT 1 cut(s) 343
BseDI CCNNGG 2 cut(s) 333, 1151
BseGI GGATG 4 cut(s) 145, 163, 466, 913
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 6 cut(s) 67, 110, 181, 651, 853, 1163
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 9 cut(s) 88, 149, 257, 316, 397, 528, 725, 1082, 1196
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 2 cut(s) 198, 1140
BsgI GTGCAG 1 cut(s) 970
BshFI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
BshNI GGYRCC 1 cut(s) 1208
BshTI ACCGGT 1 cut(s) 789
BshVI ATCGAT 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 895
BsiSI CCGG 2 cut(s) 790, 1144
BslFI GGGAC 4 cut(s) 87, 737, 794, 990
BslI CCNNNNNNNGG 6 cut(s) 67, 110, 181, 651, 853, 1163
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 4 cut(s) 87, 737, 794, 990
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
Bso31I GGTCTC 1 cut(s) 544
Bsp119I TTCGAA 1 cut(s) 769
Bsp1286I GDGCHC 1 cut(s) 895
Bsp13I TCCGGA 1 cut(s) 1143
Bsp143I GATC 2 cut(s) 589, 795
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 2 cut(s) 924, 1197
BspANI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
BspCNI CTCAG 1 cut(s) 739
BspDI ATCGAT 1 cut(s) 343
BspEI TCCGGA 1 cut(s) 1143
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 2 cut(s) 810, 1210
BspMI ACCTGC 2 cut(s) 47, 452
BspPI GGATC 2 cut(s) 597, 803
BspQI GCTCTTC 1 cut(s) 1118
BspT104I TTCGAA 1 cut(s) 769
BspT107I GGYRCC 1 cut(s) 1208
BspTI CTTAAG 1 cut(s) 571
BspTNI GGTCTC 1 cut(s) 544
BsrBI CCGCTC 1 cut(s) 926
BsrFI RCCGGY 1 cut(s) 789
BsrI ACTGG 9 cut(s) 88, 149, 257, 316, 397, 528, 725, 1082, 1196
BssAI RCCGGY 1 cut(s) 789
BssECI CCNNGG 2 cut(s) 333, 1151
BssMI GATC 2 cut(s) 589, 795
BssNI GRCGYC 1 cut(s) 1209
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 905, 1152
Bst4CI ACNGT 5 cut(s) 176, 291, 728, 899, 1004
Bst6I CTCTTC 1 cut(s) 1118
BstACI GRCGYC 1 cut(s) 1209
BstAFI CTTAAG 1 cut(s) 571
BstBAI YACGTR 1 cut(s) 547
BstBI TTCGAA 1 cut(s) 769
BstC8I GCNNGC 1 cut(s) 928
BstDEI CTNAG 1 cut(s) 747
BstDSI CCRYGG 1 cut(s) 333
BstENI CCTNNNNNAGG 2 cut(s) 65, 851
BstF5I GGATG 4 cut(s) 145, 163, 466, 913
BstH2I RGCGCY 2 cut(s) 1080, 1212
BstHHI GCGC 2 cut(s) 1079, 1211
BstKTI GATC 2 cut(s) 592, 798
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 2 cut(s) 589, 795
BstMWI GCNNNNNNNGC 3 cut(s) 516, 875, 1215
BstNI CCWGG 2 cut(s) 905, 1152
BstNSI RCATGY 3 cut(s) 534, 561, 1039
BstSCI CCNGG 2 cut(s) 903, 1150
BstV1I GCAGC 2 cut(s) 198, 1140
BstX2I RGATCY 1 cut(s) 589
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 1 cut(s) 589
Bsu15I ATCGAT 1 cut(s) 343
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
BsuTUI ATCGAT 1 cut(s) 343
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 145, 163, 466, 913
BtsIMutI CAGTG 3 cut(s) 95, 296, 895
BveI ACCTGC 2 cut(s) 47, 452
Cac8I GCNNGC 1 cut(s) 928
CciI TCATGA 1 cut(s) 238
CfoI GCGC 2 cut(s) 1079, 1211
Cfr10I RCCGGY 1 cut(s) 789
Cfr13I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
ClaI ATCGAT 1 cut(s) 343
CspAI ACCGGT 1 cut(s) 789
DdeI CTNAG 1 cut(s) 747
DinI GGCGCC 1 cut(s) 1210
DpnI GATC 2 cut(s) 591, 797
DpnII GATC 2 cut(s) 589, 795
DrdI GACNNNNNNGTC 1 cut(s) 731
DriI GACNNNNNGTC 1 cut(s) 1036
DseDI GACNNNNNNGTC 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 1118
Eam1105I GACNNNNNGTC 1 cut(s) 1036
EarI CTCTTC 1 cut(s) 1118
Eco130I CCWWGG 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 544
Eco47I GGWCC 4 cut(s) 36, 808, 1141, 1194
Eco57I CTGAAG 2 cut(s) 869, 1085
EcoNI CCTNNNNNAGG 2 cut(s) 65, 851
EcoO109I RGGNCCY 3 cut(s) 36, 643, 808
EcoRII CCWGG 2 cut(s) 903, 1150
EcoT14I CCWWGG 1 cut(s) 333
EgeI GGCGCC 1 cut(s) 1210
EheI GGCGCC 1 cut(s) 1210
ErhI CCWWGG 1 cut(s) 333
FaqI GGGAC 4 cut(s) 87, 737, 794, 990
FauNDI CATATG 1 cut(s) 27
Fnu4HI GCNGC 2 cut(s) 187, 1129
FokI GGATG 4 cut(s) 132, 170, 453, 920
Fsp4HI GCNGC 2 cut(s) 187, 1129
FspBI CTAG 2 cut(s) 837, 1203
GlaI GCGC 2 cut(s) 1078, 1210
GluI GCNGC 2 cut(s) 187, 1129
GsuI CTGGAG 2 cut(s) 71, 1065
HaeII RGCGCY 2 cut(s) 1080, 1212
HaeIII GGCC 6 cut(s) 8, 519, 644, 888, 903, 1156
HapII CCGG 2 cut(s) 790, 1144
HhaI GCGC 2 cut(s) 1079, 1211
Hin1I GRCGYC 1 cut(s) 1209
Hin6I GCGC 2 cut(s) 1077, 1209
HinP1I GCGC 2 cut(s) 1077, 1209
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 7 cut(s) 72, 100, 167, 272, 600, 743, 756
HpaII CCGG 2 cut(s) 790, 1144
HphI GGTGA 4 cut(s) 1032, 1043, 1091, 1132
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 4 cut(s) 172, 540, 748, 1059
Hpy188III TCNNGA 7 cut(s) 164, 197, 218, 239, 473, 760, 1144
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 5 cut(s) 104, 271, 670, 844, 1060
HpyCH4III ACNGT 5 cut(s) 176, 291, 728, 899, 1004
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 6 cut(s) 134, 286, 561, 585, 843, 951
HpyF10VI GCNNNNNNNGC 3 cut(s) 516, 875, 1215
HpyF3I CTNAG 1 cut(s) 747
HpySE526I ACGT 1 cut(s) 546
Hsp92I GRCGYC 1 cut(s) 1209
HspAI GCGC 2 cut(s) 1077, 1209
KasI GGCGCC 1 cut(s) 1208
Kpn2I TCCGGA 1 cut(s) 1143
Kzo9I GATC 2 cut(s) 589, 795
LguI GCTCTTC 1 cut(s) 1118
LmnI GCTCC 3 cut(s) 361, 1084, 1167
Lsp1109I GCAGC 2 cut(s) 198, 1140
LweI GCATC 2 cut(s) 121, 901
MaeI CTAG 2 cut(s) 837, 1203
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 5 cut(s) 215, 380, 628, 706, 1038
MalI GATC 2 cut(s) 591, 797
MbiI CCGCTC 1 cut(s) 926
MboI GATC 2 cut(s) 589, 795
MboII GAAGA 3 cut(s) 685, 1135, 1165
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 895
MluCI AATT 5 cut(s) 135, 191, 609, 765, 962
Mly113I GGCGCC 1 cut(s) 1209
MlyI GAGTC 2 cut(s) 109, 266
MmeI TCCRAC 2 cut(s) 230, 720
MroI TCCGGA 1 cut(s) 1143
MroXI GAANNNNTTC 2 cut(s) 114, 392
MseI TTAA 7 cut(s) 53, 126, 572, 650, 786, 803, 1243
MslI CAYNNNNRTG 1 cut(s) 985
MspCI CTTAAG 1 cut(s) 571
MspI CCGG 2 cut(s) 790, 1144
MspR9I CCNGG 2 cut(s) 905, 1152
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 2 cut(s) 905, 1152
MwoI GCNNNNNNNGC 3 cut(s) 516, 875, 1215
NarI GGCGCC 1 cut(s) 1209
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 27
NdeII GATC 2 cut(s) 589, 795
NlaIV GGNNCC 2 cut(s) 810, 1210
NmuCI GTSAC 3 cut(s) 215, 706, 1038
NspI RCATGY 3 cut(s) 534, 561, 1039
NspV TTCGAA 1 cut(s) 769
PagI TCATGA 1 cut(s) 238
PciI ACATGT 1 cut(s) 1035
PciSI GCTCTTC 1 cut(s) 1118
PctI GAATGC 1 cut(s) 307
PdmI GAANNNNTTC 2 cut(s) 114, 392
PfeI GAWTC 5 cut(s) 72, 167, 600, 743, 756
PflFI GACNNNGTC 1 cut(s) 712
PinAI ACCGGT 1 cut(s) 789
PkrI GCNGC 2 cut(s) 188, 1130
PleI GAGTC 2 cut(s) 108, 266
PluTI GGCGCC 1 cut(s) 1212
PpsI GAGTC 2 cut(s) 108, 266
Ppu21I YACGTR 1 cut(s) 547
PpuMI RGGWCCY 2 cut(s) 36, 808
PscI ACATGT 1 cut(s) 1035
Psp5II RGGWCCY 2 cut(s) 36, 808
Psp6I CCWGG 2 cut(s) 903, 1150
PspGI CCWGG 2 cut(s) 903, 1150
PspN4I GGNNCC 2 cut(s) 810, 1210
PspPI GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
PspPPI RGGWCCY 2 cut(s) 36, 808
PsuI RGATCY 1 cut(s) 589
PsyI GACNNNGTC 1 cut(s) 712
RseI CAYNNNNRTG 1 cut(s) 985
SapI GCTCTTC 1 cut(s) 1118
SaqAI TTAA 7 cut(s) 53, 126, 572, 650, 786, 803, 1243
SatI GCNGC 2 cut(s) 187, 1129
Sau3AI GATC 2 cut(s) 589, 795
Sau96I GGNCC 8 cut(s) 7, 36, 643, 808, 886, 1141, 1154, 1194
SchI GAGTC 2 cut(s) 109, 266
ScrFI CCNGG 2 cut(s) 905, 1152
SduI GDGCHC 1 cut(s) 895
SfaNI GCATC 2 cut(s) 121, 901
SfoI GGCGCC 1 cut(s) 1210
SfuI TTCGAA 1 cut(s) 769
SinI GGWCC 4 cut(s) 36, 808, 1141, 1194
SmiMI CAYNNNNRTG 1 cut(s) 985
SmlI CTYRAG 3 cut(s) 471, 571, 668
SmoI CTYRAG 3 cut(s) 471, 571, 668
Sse9I AATT 5 cut(s) 135, 191, 609, 765, 962
SsiI CCGC 2 cut(s) 924, 1197
SspDI GGCGCC 1 cut(s) 1208
SspMI CTAG 2 cut(s) 837, 1203
StyD4I CCNGG 2 cut(s) 903, 1150
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 5 cut(s) 176, 291, 728, 899, 1004
TaiI ACGT 1 cut(s) 549
TaqI TCGA 4 cut(s) 343, 483, 769, 1227
TasI AATT 5 cut(s) 135, 191, 609, 765, 962
TfiI GAWTC 5 cut(s) 72, 167, 600, 743, 756
Tru1I TTAA 7 cut(s) 53, 126, 572, 650, 786, 803, 1243
Tru9I TTAA 7 cut(s) 53, 126, 572, 650, 786, 803, 1243
TscAI CASTG 3 cut(s) 95, 296, 902
TseFI GTSAC 3 cut(s) 215, 706, 1038
TseI GCWGC 2 cut(s) 186, 1128
Tsp45I GTSAC 3 cut(s) 215, 706, 1038
TspDTI ATGAA 2 cut(s) 338, 1031
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 3 cut(s) 95, 296, 902
Tth111I GACNNNGTC 1 cut(s) 712
Vha464I CTTAAG 1 cut(s) 571
VpaK11BI GGWCC 4 cut(s) 36, 808, 1141, 1194
XagI CCTNNNNNAGG 2 cut(s) 65, 851
XapI RAATTY 4 cut(s) 191, 609, 765, 962
XceI RCATGY 3 cut(s) 534, 561, 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmnI GAANNNNTTC 2 cut(s) 114, 392
XspI CTAG 2 cut(s) 837, 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.