FvH4_7g06300

OTU domain-containing protein At3g57810-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014791
Physical Location & Seq
Forward (+)
6385123 .. 6387869
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06300.t1

Sequence Viewer

Length: 975 bp
ATGCTTGGATTTCTCTGCGCGCGCCGGAAAACTTGGATTGTCAGCTCCCTTTCTTCTTTTGCTCACGGACCGGCGGCGATTCACCAGAGCCGGATCGTCCACAGCCCTCTGATTCAGCATCAATTCACTAACTTCTCCGGCGAGACCAGGGGCCGACACCACCACAACAGCTCCTGCCAGCTAGGCTCAGCATGCGGCGGCGGCGCCGCAGCGTCCATATGGCACGCTATCCTCCCCTCCTCCGGTCTTTGGAGGCGGCGCGATCTCCGTCGTCCGGCGATCCATTACGAGCTTAAGGGTGAGGGTTCTTGGAATGCTGCTCTCGATGCTCGCCCGGCTCGCTGGCTCCACCGGCCCGACTCGGCTTGGCTCCTTTTCGGGGTTTGCAACTGCCTGGCGCCGATAGACTGGGGCAGTACAACTAACTCAACCACCAACGATGAGGTGTCCAATAACAAAACTGAAGCCTGTGATTCGAAAAGCTCAATTACTTCGGATGTGCAGTTAGAGACGCCTGATTACAGAGTTACAGGAGTGCTAGCTGATGGTCGGTGTCTCTTCAGAGCGATAGCACATGTGGCTTGCTTAAGAAATGGGGAGGAACCTCCTGATGAAAACCGACAAAGAGAGCTCGCTGATGAATTAAGAGCTCAAGTTGTCGATGAGCTTTTAAAGAGGCGGGAGGAAACTGAATGGTTCATTGAAGGTGATTTCGATGCATATGTGAAGAGAATTCAACAACCTTATGTTTGGGGAGGAGAACCTGAATTGCTTATGGCTTCTCATGTTAAAAAGGCACCAATATCAGTCTACATGGTAGATAGAAGCTCGGGTGGTTTGGTAAACATAGCAAAATATGGTGAAGAGTATGGGAAACAAGAAGAGAAACCAATCAATGTTCTATTTCATGGTTATGGTCACTATGACATCTTGGAGTCTTTCTCAGAACAGAGTCTGCAGAAAGTAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

325

Amino Acids

36.36

Weight (kDa)

6.4

Isoelectric Point (pI)

63.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 181 - 304 8.5e-21 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014791)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06300
malus_domestica MD02G1245100.v1.1
prunus_persica Prupe.2G089600_v2.0.a1 Prupe.2G089600_v2.0.a1
pyrus_communis pycom02g21100
rosa_chinensis RchiOBHm_Chr1g0334131
rosa_laevigata RLG00000029477
rosa_multiflora Rmu_sc0003690.1_g000029 Rmu_sc0008401.1_g000011
rosa_roxburghii Rroxscaffold_4G00316990
rosa_rugosa Rorug01G0112900
rosa_samantha Rh1AG136900 Rh1BG104300 Rh1CG129100 Rh1DG141900
rosa_wichuraiana Rw0G019310 Rw1G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 203, 397, 796
AccI GTMKAC 1 cut(s) 810
AccII CGCG 3 cut(s) 20, 22, 261
AciI CCGC 7 cut(s) 74, 195, 198, 201, 207, 256, 679
AclWI GGATC 2 cut(s) 101, 274
AcsI RAATTY 1 cut(s) 732
AcuI CTGAAG 2 cut(s) 483, 544
AcyI GRCGYC 3 cut(s) 204, 398, 512
AfaI GTAC 1 cut(s) 418
AfiI CCNNNNNNNGG 4 cut(s) 242, 249, 274, 379
AflII CTTAAG 2 cut(s) 293, 586
AflIII ACRYGT 2 cut(s) 574, 969
AgsI TTSAA 2 cut(s) 704, 737
AjnI CCWGG 2 cut(s) 146, 393
Alw21I GWGCWC 2 cut(s) 633, 652
Alw26I GTCTC 3 cut(s) 137, 503, 560
AlwI GGATC 2 cut(s) 101, 274
AlwNI CAGNNNCTG 2 cut(s) 174, 955
Ama87I CYCGRG 1 cut(s) 829
AoxI GGCC 2 cut(s) 151, 353
ApeKI GCWGC 2 cut(s) 209, 317
ApoI RAATTY 1 cut(s) 732
AspLEI GCGC 6 cut(s) 20, 22, 24, 206, 261, 400
AspS9I GGNCC 3 cut(s) 68, 151, 354
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 4 cut(s) 74, 311, 719, 872
AsuII TTCGAA 1 cut(s) 476
AsuNHI GCTAGC 1 cut(s) 538
AvaI CYCGRG 1 cut(s) 829
AvaII GGWCC 1 cut(s) 68
BanI GGYRCC 3 cut(s) 203, 397, 796
BanII GRGCYC 2 cut(s) 633, 652
Bbv12I GWGCWC 2 cut(s) 633, 652
BbvI GCAGC 2 cut(s) 221, 304
BccI CCATC 1 cut(s) 539
BciT130I CCWGG 2 cut(s) 148, 395
BcnI CCSGG 1 cut(s) 335
BcoDI GTCTC 3 cut(s) 137, 503, 560
BfaI CTAG 2 cut(s) 182, 539
BfmI CTRYAG 1 cut(s) 956
BfoI RGCGCY 2 cut(s) 207, 401
BfrI CTTAAG 2 cut(s) 293, 586
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 8 cut(s) 75, 196, 199, 202, 207, 210, 257, 318
BlpI GCTNAGC 1 cut(s) 187
BlsI GCNGC 8 cut(s) 76, 197, 200, 203, 208, 211, 258, 319
Bme1390I CCNGG 3 cut(s) 148, 335, 395
Bme18I GGWCC 1 cut(s) 68
BmeT110I CYCGRG 1 cut(s) 829
BmgT120I GGNCC 3 cut(s) 68, 151, 354
BmiI GGNNCC 7 cut(s) 152, 205, 347, 371, 399, 603, 798
BmrFI CCNGG 3 cut(s) 148, 335, 395
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 3 cut(s) 127, 316, 706
BmtI GCTAGC 1 cut(s) 542
BmuI ACTGGG 1 cut(s) 418
BplI GAGNNNNNCTC 2 cut(s) 926, 958
Bpu1102I GCTNAGC 1 cut(s) 187
Bpu14I TTCGAA 1 cut(s) 476
BpuEI CTTGAG 1 cut(s) 636
BpuMI CCSGG 1 cut(s) 335
BsaHI GRCGYC 3 cut(s) 204, 398, 512
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 4 cut(s) 242, 249, 274, 379
Bse118I RCCGGY 2 cut(s) 70, 351
Bse1I ACTGG 1 cut(s) 413
BseBI CCWGG 2 cut(s) 148, 395
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 1 cut(s) 502
BseLI CCNNNNNNNGG 4 cut(s) 242, 249, 274, 379
BseMII CTCAG 2 cut(s) 201, 957
BseNI ACTGG 1 cut(s) 413
BsePI GCGCGC 2 cut(s) 18, 20
BseRI GAGGAG 2 cut(s) 229, 771
BseXI GCAGC 2 cut(s) 221, 304
BsgI GTGCAG 1 cut(s) 521
Bsh1236I CGCG 3 cut(s) 20, 22, 261
BshFI GGCC 2 cut(s) 153, 355
BshNI GGYRCC 3 cut(s) 203, 397, 796
BsiHKAI GWGCWC 2 cut(s) 633, 652
BsiHKCI CYCGRG 1 cut(s) 829
BsiSI CCGG 8 cut(s) 25, 71, 91, 138, 243, 275, 335, 352
BslI CCNNNNNNNGG 4 cut(s) 242, 249, 274, 379
BsmAI GTCTC 3 cut(s) 137, 503, 560
BsmBI CGTCTC 1 cut(s) 503
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 2 cut(s) 153, 355
Bso31I GGTCTC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 829
Bsp119I TTCGAA 1 cut(s) 476
Bsp1286I GDGCHC 2 cut(s) 633, 652
Bsp143I GATC 3 cut(s) 93, 262, 279
Bsp1720I GCTNAGC 1 cut(s) 187
BspACI CCGC 7 cut(s) 74, 195, 198, 201, 207, 256, 679
BspANI GGCC 2 cut(s) 153, 355
BspCNI CTCAG 2 cut(s) 200, 956
BspFNI CGCG 3 cut(s) 20, 22, 261
BspLI GGNNCC 7 cut(s) 152, 205, 347, 371, 399, 603, 798
BspMAI CTGCAG 1 cut(s) 960
BspOI GCTAGC 1 cut(s) 542
BspPI GGATC 2 cut(s) 101, 274
BspT104I TTCGAA 1 cut(s) 476
BspT107I GGYRCC 3 cut(s) 203, 397, 796
BspTI CTTAAG 2 cut(s) 293, 586
BspTNI GGTCTC 1 cut(s) 137
BsrFI RCCGGY 2 cut(s) 70, 351
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 2 cut(s) 70, 351
BssECI CCNNGG 1 cut(s) 147
BssHII GCGCGC 2 cut(s) 18, 20
BssMI GATC 3 cut(s) 93, 262, 279
BssNI GRCGYC 3 cut(s) 204, 398, 512
Bst2UI CCWGG 2 cut(s) 148, 395
Bst6I CTCTTC 4 cut(s) 563, 722, 858, 876
BstACI GRCGYC 3 cut(s) 204, 398, 512
BstAFI CTTAAG 2 cut(s) 293, 586
BstBI TTCGAA 1 cut(s) 476
BstDEI CTNAG 2 cut(s) 187, 943
BstF5I GGATG 1 cut(s) 502
BstFNI CGCG 3 cut(s) 20, 22, 261
BstH2I RGCGCY 2 cut(s) 207, 401
BstHHI GCGC 6 cut(s) 20, 22, 24, 206, 261, 400
BstKTI GATC 3 cut(s) 96, 265, 282
BstMAI GTCTC 3 cut(s) 137, 503, 560
BstMBI GATC 3 cut(s) 93, 262, 279
BstMWI GCNNNNNNNGC 8 cut(s) 183, 192, 201, 326, 335, 339, 352, 578
BstNI CCWGG 2 cut(s) 148, 395
BstNSI RCATGY 3 cut(s) 195, 578, 973
BstSCI CCNGG 3 cut(s) 146, 333, 393
BstSFI CTRYAG 1 cut(s) 956
BstUI CGCG 3 cut(s) 20, 22, 261
BstV1I GCAGC 2 cut(s) 221, 304
BsuRI GGCC 2 cut(s) 153, 355
BtsCI GGATG 1 cut(s) 502
CaiI CAGNNNCTG 2 cut(s) 174, 955
CfoI GCGC 6 cut(s) 20, 22, 24, 206, 261, 400
Cfr10I RCCGGY 2 cut(s) 70, 351
Cfr13I GGNCC 3 cut(s) 68, 151, 354
CpoI CGGWCCG 1 cut(s) 68
CseI GACGC 2 cut(s) 201, 520
Csp6I GTAC 1 cut(s) 417
CspI CGGWCCG 1 cut(s) 68
CviAII CATG 6 cut(s) 192, 575, 785, 814, 908, 970
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 2 cut(s) 187, 943
DinI GGCGCC 2 cut(s) 205, 399
DpnI GATC 3 cut(s) 95, 264, 281
DpnII GATC 3 cut(s) 93, 262, 279
DraI TTTAAA 1 cut(s) 672
Eam1104I CTCTTC 4 cut(s) 563, 722, 858, 876
EarI CTCTTC 4 cut(s) 563, 722, 858, 876
Ecl136II GAGCTC 2 cut(s) 631, 650
Eco24I GRGCYC 2 cut(s) 633, 652
Eco31I GGTCTC 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 68
Eco53kI GAGCTC 2 cut(s) 631, 650
Eco57I CTGAAG 2 cut(s) 483, 544
Eco88I CYCGRG 1 cut(s) 829
EcoICRI GAGCTC 2 cut(s) 631, 650
EcoRI GAATTC 1 cut(s) 732
EcoRII CCWGG 2 cut(s) 146, 393
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 2 cut(s) 633, 652
EgeI GGCGCC 2 cut(s) 205, 399
EheI GGCGCC 2 cut(s) 205, 399
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 6 cut(s) 195, 578, 788, 817, 911, 973
FatI CATG 6 cut(s) 191, 574, 784, 813, 907, 969
FauI CCCGC 1 cut(s) 672
FauNDI CATATG 2 cut(s) 218, 721
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 8 cut(s) 75, 196, 199, 202, 207, 210, 257, 318
FokI GGATG 1 cut(s) 509
FriOI GRGCYC 2 cut(s) 633, 652
Fsp4HI GCNGC 8 cut(s) 75, 196, 199, 202, 207, 210, 257, 318
FspBI CTAG 2 cut(s) 182, 539
GlaI GCGC 6 cut(s) 19, 21, 23, 205, 260, 399
GluI GCNGC 8 cut(s) 75, 196, 199, 202, 207, 210, 257, 318
HaeII RGCGCY 2 cut(s) 207, 401
HaeIII GGCC 2 cut(s) 153, 355
HapII CCGG 8 cut(s) 25, 71, 91, 138, 243, 275, 335, 352
HgaI GACGC 2 cut(s) 201, 520
HhaI GCGC 6 cut(s) 20, 22, 24, 206, 261, 400
Hin1I GRCGYC 3 cut(s) 204, 398, 512
Hin1II CATG 6 cut(s) 195, 578, 788, 817, 911, 973
Hin6I GCGC 6 cut(s) 18, 20, 22, 204, 259, 398
HinP1I GCGC 6 cut(s) 18, 20, 22, 204, 259, 398
HinfI GANTC 6 cut(s) 79, 112, 359, 473, 935, 952
HpaII CCGG 8 cut(s) 25, 71, 91, 138, 243, 275, 335, 352
HphI GGTGA 4 cut(s) 74, 311, 719, 872
Hpy166II GTNNAC 4 cut(s) 100, 811, 844, 967
Hpy188I TCNGA 4 cut(s) 111, 496, 563, 946
Hpy188III TCNNGA 2 cut(s) 323, 608
Hpy8I GTNNAC 4 cut(s) 100, 811, 844, 967
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 1 cut(s) 698
HpyCH4V TGCA 4 cut(s) 387, 502, 719, 958
HpyF10VI GCNNNNNNNGC 8 cut(s) 183, 192, 201, 326, 335, 339, 352, 578
HpyF3I CTNAG 2 cut(s) 187, 943
Hsp92I GRCGYC 3 cut(s) 204, 398, 512
Hsp92II CATG 6 cut(s) 195, 578, 788, 817, 911, 973
HspAI GCGC 6 cut(s) 18, 20, 22, 204, 259, 398
KasI GGCGCC 2 cut(s) 203, 397
Kzo9I GATC 3 cut(s) 93, 262, 279
LmnI GCTCC 4 cut(s) 50, 176, 351, 375
Lsp1109I GCAGC 2 cut(s) 221, 304
LweI GCATC 3 cut(s) 127, 316, 706
MaeI CTAG 2 cut(s) 182, 539
MaeIII GTNAC 2 cut(s) 526, 917
MalI GATC 3 cut(s) 95, 264, 281
MboI GATC 3 cut(s) 93, 262, 279
MboII GAAGA 5 cut(s) 45, 550, 739, 875, 893
MhlI GDGCHC 2 cut(s) 633, 652
MluCI AATT 5 cut(s) 122, 486, 641, 732, 767
Mly113I GGCGCC 2 cut(s) 204, 398
MlyI GAGTC 3 cut(s) 353, 944, 961
Mph1103I ATGCAT 1 cut(s) 721
MseI TTAA 5 cut(s) 294, 587, 644, 671, 789
MslI CAYNNNNRTG 1 cut(s) 912
MspCI CTTAAG 2 cut(s) 293, 586
MspI CCGG 8 cut(s) 25, 71, 91, 138, 243, 275, 335, 352
MspR9I CCNGG 3 cut(s) 148, 335, 395
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 2 cut(s) 148, 395
MvnI CGCG 3 cut(s) 20, 22, 261
MwoI GCNNNNNNNGC 8 cut(s) 183, 192, 201, 326, 335, 339, 352, 578
NarI GGCGCC 2 cut(s) 204, 398
NciI CCSGG 1 cut(s) 335
NdeI CATATG 2 cut(s) 218, 721
NdeII GATC 3 cut(s) 93, 262, 279
NheI GCTAGC 1 cut(s) 538
NlaIII CATG 6 cut(s) 195, 578, 788, 817, 911, 973
NlaIV GGNNCC 7 cut(s) 152, 205, 347, 371, 399, 603, 798
NmeAIII GCCGAG 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 917
NsiI ATGCAT 1 cut(s) 721
NspI RCATGY 3 cut(s) 195, 578, 973
NspV TTCGAA 1 cut(s) 476
PaeI GCATGC 1 cut(s) 195
PauI GCGCGC 2 cut(s) 18, 20
PciI ACATGT 2 cut(s) 574, 969
PctI GAATGC 1 cut(s) 319
PfeI GAWTC 3 cut(s) 79, 112, 473
PkrI GCNGC 8 cut(s) 76, 197, 200, 203, 208, 211, 258, 319
PleI GAGTC 3 cut(s) 353, 943, 960
PluTI GGCGCC 2 cut(s) 207, 401
PpsI GAGTC 3 cut(s) 353, 943, 960
PscI ACATGT 2 cut(s) 574, 969
Psp124BI GAGCTC 2 cut(s) 633, 652
Psp6I CCWGG 2 cut(s) 146, 393
PspGI CCWGG 2 cut(s) 146, 393
PspN4I GGNNCC 7 cut(s) 152, 205, 347, 371, 399, 603, 798
PspPI GGNCC 3 cut(s) 68, 151, 354
PstI CTGCAG 1 cut(s) 960
PstNI CAGNNNCTG 2 cut(s) 174, 955
PteI GCGCGC 2 cut(s) 18, 20
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
RseI CAYNNNNRTG 1 cut(s) 912
Rsr2I CGGWCCG 1 cut(s) 68
RsrII CGGWCCG 1 cut(s) 68
SacI GAGCTC 2 cut(s) 633, 652
SaqAI TTAA 5 cut(s) 294, 587, 644, 671, 789
SatI GCNGC 8 cut(s) 75, 196, 199, 202, 207, 210, 257, 318
Sau3AI GATC 3 cut(s) 93, 262, 279
Sau96I GGNCC 3 cut(s) 68, 151, 354
SchI GAGTC 3 cut(s) 353, 944, 961
ScrFI CCNGG 3 cut(s) 148, 335, 395
SduI GDGCHC 2 cut(s) 633, 652
SfaNI GCATC 3 cut(s) 127, 316, 706
SfcI CTRYAG 1 cut(s) 956
SfoI GGCGCC 2 cut(s) 205, 399
SfuI TTCGAA 1 cut(s) 476
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 3 cut(s) 293, 586, 651
SmoI CTYRAG 3 cut(s) 293, 586, 651
SphI GCATGC 1 cut(s) 195
Sse9I AATT 5 cut(s) 122, 486, 641, 732, 767
SsiI CCGC 7 cut(s) 74, 195, 198, 201, 207, 256, 679
SspDI GGCGCC 2 cut(s) 203, 397
SspMI CTAG 2 cut(s) 182, 539
SstI GAGCTC 2 cut(s) 633, 652
StyD4I CCNGG 3 cut(s) 146, 333, 393
TaqI TCGA 4 cut(s) 324, 476, 660, 714
TasI AATT 5 cut(s) 122, 486, 641, 732, 767
TatI WGTACW 1 cut(s) 416
TauI GCSGC 6 cut(s) 77, 198, 201, 204, 209, 259
TfiI GAWTC 3 cut(s) 79, 112, 473
Tru1I TTAA 5 cut(s) 294, 587, 644, 671, 789
Tru9I TTAA 5 cut(s) 294, 587, 644, 671, 789
TseFI GTSAC 1 cut(s) 917
TseI GCWGC 2 cut(s) 209, 317
Tsp45I GTSAC 1 cut(s) 917
TspDTI ATGAA 4 cut(s) 627, 654, 688, 896
TspGWI ACGGA 2 cut(s) 81, 257
Vha464I CTTAAG 2 cut(s) 293, 586
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 1 cut(s) 732
XceI RCATGY 3 cut(s) 195, 578, 973
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 2 cut(s) 182, 539
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.