FvH4_7g08130

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015815
Physical Location & Seq
Forward (+)
8086215 .. 8091786
5572 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08130.t2

Sequence Viewer

Length: 1392 bp
ATGGCCGGTGGAGGCGAAGGTCCAGATTTAGAGTGGACTCCGACATGGGTGGTTGCCATAGTGTGCTCTGTGATAGTTGTTATCTCTCTTATGTTAGAGAGAGGCCTTCACTCGGCCGGGAACTACTTGAAAAGGAAGGAACAAAATCCGTTGTATGAGGCGTTGCTCAAGGTGAAGGAGGAGTTGATGTTGGTAGGGTTTATATCTCTGCTACTGACCGTGTTCACGAAAACCATCACCAAAATCTGCATCAAGGAGTCGAAAATGGATAAATGGCTGCCATGTCCACTCGGCGAGGAACCACAGGACATGGACGATTTGGATCAAGATACCCTGTTGAAGCTTGACGATGAATTGAGTTACTGTGCCAGAAAGGGTAAGGTACCAGTGTTATCAGCTGAAGCTGTCCATCAATTGCATATCTTTATATTTACCCTAGCCATTGTTCATGTAACCTTCTCTGCTATGACTATTTTCATCGGTGGGTTAAAGATACGCCAATGGAAACAGTGGGAGGAGTTAGTTGCAAAAGAGATTAGTGAAAAGGAATCTGTTGGTAGTGCAGACGAAGCAAAGGTTACAAATGTTCAAGAGCATGATTTTGTCAAAGATCATTACTTGGGTATCGGTGAAAATATTCGAATCAAGGGTTGGATACATTCTTTTAAAAACCAATTTTATGGATCTGTGACCAAATCAGATTATCTAACTTTGCGTCTAGGCTTCATCTCGACTCATTGCAAGAACAACCCCAAATTTGATTTTCACACTTACATGGTGCGTACTCTTGAAGATGATTTTAGAAAAGTTGTTGGTATCAGTTGGTATCTATGGATATTCGTGGTGTTTTTTTTGCTGATCAATATTGATGGCGTGCATGCGTACTTCTGGCTAGCTTTCATCCCTTTCTTTCTTCTACTTGCTACAGGAACTAAGTTGCAGCATATAATCACCCAATTGGCTCACGAGGTTGCTGAGAAACATGTAGCAGTAGAAGGGGATCTATTAGTTAAACCATCAGACCAACATTTTTGGTTTGGTCGACCCAGTTTTGTTCTCTTCTTGATCCATTTCATCCTTTTCCAAAATGCTTTCGAGATGGCATTTTTCCTTTGGATATGGGCTCAATATGGTTTTAGGTCTTGCATAATGGGAAAAGCCGAATACATTTTCCCTCGGATTCTTATAGGGACGTTCATTCAGTTCCTCACTAGTTACAGCACTCTACCACTTTATGCCATTGTCACACAGATGGGATCTAATTTCAAGAAAGAAATATTCGTTGAGGGATCGGTACATACGACGAGAAGGAACCAAAGTAATTGGTCTCACATAGCGACGAGAAGGAAAACAAGTCCTGATGGTAATGGGGAAGTTCAACTGCAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

464

Amino Acids

53.28

Weight (kDa)

6.79

Isoelectric Point (pI)

28.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 451 7.4e-172 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015815)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08130 FvH4_7g08130
malus_domestica MD02G1230200.v1.1 MD07G1082000.v1.1
prunus_persica Prupe.2G107600_v2.0.a1 Prupe.2G107600_v2.0.a1
pyrus_communis pycom07g06530
rosa_chinensis RchiOBHm_Chr1g0341101
rosa_laevigata RLG00000029064
rosa_multiflora Rmu_sc0001211.1_g000067
rosa_rugosa Rorug01G0159900.1
rosa_samantha Rh1AG174200 Rh1BG142500 Rh1CG162000
rosa_wichuraiana Rw1G014530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 382
AccB1I GGYRCC 1 cut(s) 382
AccI GTMKAC 1 cut(s) 1042
AclWI GGATC 6 cut(s) 330, 691, 1008, 1060, 1264, 1297
AcoI YGGCCR 2 cut(s) 3, 114
AcsI RAATTY 1 cut(s) 755
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 4 cut(s) 384, 784, 884, 1296
AfiI CCNNNNNNNGG 1 cut(s) 112
AflIII ACRYGT 1 cut(s) 982
AgsI TTSAA 6 cut(s) 130, 340, 590, 791, 1267, 1379
AhlI ACTAGT 1 cut(s) 1211
AjuI GAANNNNNNNTTGG 2 cut(s) 634, 666
AluBI AGCT 4 cut(s) 343, 398, 404, 896
AluI AGCT 4 cut(s) 343, 398, 404, 896
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 1 cut(s) 1332
AlwI GGATC 6 cut(s) 330, 691, 1008, 1060, 1264, 1297
AoxI GGCC 3 cut(s) 3, 103, 114
ApeKI GCWGC 2 cut(s) 277, 940
ApoI RAATTY 1 cut(s) 755
Asp700I GAANNNNTTC 1 cut(s) 636
Asp718I GGTACC 1 cut(s) 382
AspS9I GGNCC 1 cut(s) 20
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 4 cut(s) 184, 229, 641, 943
AsuII TTCGAA 1 cut(s) 640
AsuNHI GCTAGC 1 cut(s) 892
AvaII GGWCC 1 cut(s) 20
BaeI ACNNNNGTAYC 4 cut(s) 374, 407, 607, 640
BanI GGYRCC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 1126
BauI CACGAG 1 cut(s) 965
Bbv12I GWGCWC 1 cut(s) 68
BbvI GCAGC 2 cut(s) 264, 952
BccI CCATC 7 cut(s) 242, 417, 863, 1024, 1093, 1246, 1355
BciVI GTATCC 1 cut(s) 648
BclI TGATCA 1 cut(s) 858
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 1332
BcuI ACTAGT 1 cut(s) 1211
BfaI CTAG 4 cut(s) 437, 719, 893, 1212
BfmI CTRYAG 1 cut(s) 924
BfuI GTATCC 1 cut(s) 648
BisI GCNGC 2 cut(s) 278, 941
BlsI GCNGC 2 cut(s) 279, 942
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 3 cut(s) 300, 384, 1313
BmrFI CCNGG 1 cut(s) 118
BmrI ACTGGG 1 cut(s) 1041
BmsI GCATC 1 cut(s) 258
BmtI GCTAGC 1 cut(s) 896
BmuI ACTGGG 1 cut(s) 1041
Bpu14I TTCGAA 1 cut(s) 640
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 321
BsaI GGTCTC 1 cut(s) 1332
BsaJI CCNNGG 1 cut(s) 1175
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 2 cut(s) 386, 1047
Bse3DI GCAATG 1 cut(s) 736
Bse8I GATNNNNATC 1 cut(s) 321
BseDI CCNNGG 1 cut(s) 1175
BseGI GGATG 2 cut(s) 900, 1074
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 112
BseMI GCAATG 1 cut(s) 736
BseMII CTCAG 1 cut(s) 966
BseNI ACTGG 2 cut(s) 386, 1047
BseRI GAGGAG 2 cut(s) 194, 530
BseX3I CGGCCG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 264, 952
BsgI GTGCAG 1 cut(s) 582
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 3 cut(s) 5, 105, 116
BshNI GGYRCC 1 cut(s) 382
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 68
BsiSI CCGG 2 cut(s) 6, 117
BslFI GGGAC 1 cut(s) 1204
BslI CCNNNNNNNGG 1 cut(s) 112
BsmAI GTCTC 1 cut(s) 1332
BsmFI GGGAC 1 cut(s) 1204
BsnI GGCC 3 cut(s) 5, 105, 116
Bso31I GGTCTC 1 cut(s) 1332
Bsp119I TTCGAA 1 cut(s) 640
Bsp1286I GDGCHC 2 cut(s) 68, 1126
Bsp143I GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
BspANI GGCC 3 cut(s) 5, 105, 116
BspCNI CTCAG 1 cut(s) 967
BspLI GGNNCC 3 cut(s) 300, 384, 1313
BspOI GCTAGC 1 cut(s) 896
BspPI GGATC 6 cut(s) 330, 691, 1008, 1060, 1264, 1297
BspT104I TTCGAA 1 cut(s) 640
BspT107I GGYRCC 1 cut(s) 382
BspTNI GGTCTC 1 cut(s) 1332
BsrDI GCAATG 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 2 cut(s) 386, 1047
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 1175
BssMI GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
BssSI CACGAG 1 cut(s) 965
Bst2BI CACGAG 1 cut(s) 965
Bst4CI ACNGT 3 cut(s) 220, 365, 510
Bst6I CTCTTC 1 cut(s) 1064
BstBI TTCGAA 1 cut(s) 640
BstC8I GCNNGC 3 cut(s) 875, 879, 894
BstDEI CTNAG 2 cut(s) 933, 975
BstF5I GGATG 2 cut(s) 900, 1074
BstKTI GATC 8 cut(s) 325, 613, 686, 861, 1003, 1068, 1259, 1292
BstMAI GTCTC 1 cut(s) 1332
BstMBI GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 1 cut(s) 569
BstNSI RCATGY 2 cut(s) 881, 986
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 1 cut(s) 924
BstV1I GCAGC 2 cut(s) 264, 952
BstX2I RGATCY 3 cut(s) 683, 1000, 1256
BstXI CCANNNNNNTGG 1 cut(s) 680
BstYI RGATCY 3 cut(s) 683, 1000, 1256
BstZI CGGCCG 1 cut(s) 114
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 3 cut(s) 5, 105, 116
BtsCI GGATG 2 cut(s) 900, 1074
BtsIMutI CAGTG 2 cut(s) 393, 515
Cac8I GCNNGC 3 cut(s) 875, 879, 894
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 20
CseI GACGC 1 cut(s) 704
Csp6I GTAC 4 cut(s) 383, 783, 883, 1295
CviAII CATG 8 cut(s) 45, 282, 310, 449, 596, 775, 878, 983
CviQI GTAC 4 cut(s) 383, 783, 883, 1295
DdeI CTNAG 2 cut(s) 933, 975
DpnI GATC 8 cut(s) 324, 612, 685, 860, 1002, 1067, 1258, 1291
DpnII GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
DraI TTTAAA 1 cut(s) 667
EaeI YGGCCR 2 cut(s) 3, 114
EagI CGGCCG 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 1064
EarI CTCTTC 1 cut(s) 1064
EclXI CGGCCG 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 1126
Eco31I GGTCTC 1 cut(s) 1332
Eco47I GGWCC 1 cut(s) 20
Eco52I CGGCCG 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 1126
FaeI CATG 8 cut(s) 48, 285, 313, 452, 599, 778, 881, 986
FaqI GGGAC 1 cut(s) 1204
FatI CATG 8 cut(s) 44, 281, 309, 448, 595, 774, 877, 982
FbaI TGATCA 1 cut(s) 858
FblI GTMKAC 1 cut(s) 1042
Fnu4HI GCNGC 2 cut(s) 278, 941
FokI GGATG 2 cut(s) 887, 1061
FriOI GRGCYC 1 cut(s) 1126
Fsp4HI GCNGC 2 cut(s) 278, 941
FspBI CTAG 4 cut(s) 437, 719, 893, 1212
GluI GCNGC 2 cut(s) 278, 941
HaeIII GGCC 3 cut(s) 5, 105, 116
HapII CCGG 2 cut(s) 6, 117
HgaI GACGC 1 cut(s) 704
Hin1II CATG 8 cut(s) 48, 285, 313, 452, 599, 778, 881, 986
HincII GTYRAC 1 cut(s) 1043
HindII GTYRAC 1 cut(s) 1043
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 6 cut(s) 37, 257, 548, 642, 733, 1180
HpaII CCGG 2 cut(s) 6, 117
HphI GGTGA 4 cut(s) 184, 229, 641, 943
Hpy166II GTNNAC 4 cut(s) 36, 225, 287, 1043
Hpy188I TCNGA 4 cut(s) 42, 700, 1021, 1179
Hpy8I GTNNAC 4 cut(s) 36, 225, 287, 1043
Hpy99I CGWCG 2 cut(s) 1306, 1342
HpyAV CCTTC 8 cut(s) 11, 116, 130, 169, 466, 989, 1302, 1338
HpyCH4III ACNGT 3 cut(s) 220, 365, 510
HpyCH4IV ACGT 1 cut(s) 1193
HpyCH4V TGCA 9 cut(s) 249, 418, 527, 563, 741, 877, 940, 1146, 1384
HpyF10VI GCNNNNNNNGC 1 cut(s) 569
HpyF3I CTNAG 2 cut(s) 933, 975
HpySE526I ACGT 1 cut(s) 1193
Hsp92II CATG 8 cut(s) 48, 285, 313, 452, 599, 778, 881, 986
KpnI GGTACC 1 cut(s) 386
Ksp22I TGATCA 1 cut(s) 858
Kzo9I GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
Lsp1109I GCAGC 2 cut(s) 264, 952
LweI GCATC 1 cut(s) 258
MaeI CTAG 4 cut(s) 437, 719, 893, 1212
MaeII ACGT 1 cut(s) 1193
MaeIII GTNAC 6 cut(s) 359, 451, 577, 688, 1214, 1243
MalI GATC 8 cut(s) 324, 612, 685, 860, 1002, 1067, 1258, 1291
MboI GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
MboII GAAGA 3 cut(s) 803, 905, 1051
MfeI CAATTG 2 cut(s) 413, 956
MflI RGATCY 3 cut(s) 683, 1000, 1256
MhlI GDGCHC 2 cut(s) 68, 1126
MluCI AATT 7 cut(s) 353, 413, 674, 755, 956, 1261, 1321
MlyI GAGTC 3 cut(s) 31, 266, 727
MmeI TCCRAC 2 cut(s) 65, 632
MroXI GAANNNNTTC 1 cut(s) 636
MseI TTAA 3 cut(s) 488, 666, 1011
MslI CAYNNNNRTG 2 cut(s) 773, 1250
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 2 cut(s) 6, 117
MspR9I CCNGG 1 cut(s) 118
MunI CAATTG 2 cut(s) 413, 956
MwoI GCNNNNNNNGC 1 cut(s) 569
NciI CCSGG 1 cut(s) 118
NdeII GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
NheI GCTAGC 1 cut(s) 892
NlaIII CATG 8 cut(s) 48, 285, 313, 452, 599, 778, 881, 986
NlaIV GGNNCC 3 cut(s) 300, 384, 1313
NmeAIII GCCGAG 2 cut(s) 92, 270
NmuCI GTSAC 2 cut(s) 688, 1243
NspI RCATGY 2 cut(s) 881, 986
NspV TTCGAA 1 cut(s) 640
PaeI GCATGC 1 cut(s) 881
PceI AGGCCT 1 cut(s) 105
PciI ACATGT 1 cut(s) 982
PcsI WCGNNNNNNNCGW 1 cut(s) 1298
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 3 cut(s) 548, 642, 1180
PkrI GCNGC 2 cut(s) 279, 942
PleI GAGTC 3 cut(s) 31, 265, 727
PpsI GAGTC 3 cut(s) 31, 265, 727
PscI ACATGT 1 cut(s) 982
PspN4I GGNNCC 3 cut(s) 300, 384, 1313
PspPI GGNCC 1 cut(s) 20
PsuI RGATCY 3 cut(s) 683, 1000, 1256
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 4 cut(s) 384, 784, 884, 1296
RsaNI GTAC 4 cut(s) 383, 783, 883, 1295
RseI CAYNNNNRTG 2 cut(s) 773, 1250
SalI GTCGAC 1 cut(s) 1041
SaqAI TTAA 3 cut(s) 488, 666, 1011
SatI GCNGC 2 cut(s) 278, 941
Sau3AI GATC 8 cut(s) 322, 610, 683, 858, 1000, 1065, 1256, 1289
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 3 cut(s) 31, 266, 727
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 2 cut(s) 68, 1126
SfaNI GCATC 1 cut(s) 258
SfcI CTRYAG 1 cut(s) 924
SfuI TTCGAA 1 cut(s) 640
SinI GGWCC 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 773, 1250
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SpeI ACTAGT 1 cut(s) 1211
SphI GCATGC 1 cut(s) 881
Sse9I AATT 7 cut(s) 353, 413, 674, 755, 956, 1261, 1321
SseBI AGGCCT 1 cut(s) 105
SspI AATATT 3 cut(s) 637, 865, 1278
SspMI CTAG 4 cut(s) 437, 719, 893, 1212
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 3 cut(s) 220, 365, 510
TaiI ACGT 1 cut(s) 1196
TaqI TCGA 5 cut(s) 260, 640, 731, 1042, 1095
TasI AATT 7 cut(s) 353, 413, 674, 755, 956, 1261, 1321
TfiI GAWTC 3 cut(s) 548, 642, 1180
Tru1I TTAA 3 cut(s) 488, 666, 1011
Tru9I TTAA 3 cut(s) 488, 666, 1011
TscAI CASTG 2 cut(s) 393, 515
TseFI GTSAC 2 cut(s) 688, 1243
TseI GCWGC 2 cut(s) 277, 940
Tsp45I GTSAC 2 cut(s) 688, 1243
TspDTI ATGAA 7 cut(s) 366, 437, 466, 715, 889, 1063, 1186
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 2 cut(s) 393, 515
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 1 cut(s) 755
XceI RCATGY 2 cut(s) 881, 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 30
XmiI GTMKAC 1 cut(s) 1042
XmnI GAANNNNTTC 1 cut(s) 636
XspI CTAG 4 cut(s) 437, 719, 893, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.