MD02G1230200.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015815
Physical Location & Seq
Forward (+)
27623258 .. 27631421
8164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1230200.v1.1.491

Sequence Viewer

Length: 1401 bp
ATGGCTGGTGGTGGAGAAGGTCCAACGCTACAGTTCACGCCAACATGGGTGGTGGCGGCGGTGTGCACGCTGATCGTGGCTATCTCTCTCGCTATGGAGCGTGCACTACACTCCGGCGGCAAGTACTTGAAGAAGAAGAAGCAGAAGCCGCTGTACGAGGCTTTGCTGAAGGTGAAGGAGGAGCTGATGCTGCTGGGGTTCATCTCTCTCCTCCTCACTGTTTTTCAGAAGAGGATCACTAAAATTTGCGTGAGCCCTAGGATTATGGTCAAGTGGCTGCCATGCAAACTCAATCACTATGACGATCATCTGAACGGCGAAAAAACTGCCCATTACTCAGCCAACTCCGTGTTTGGTGGCATTCTTGGACCTACCAGGAGGCTGCTAGCTGCCGGGGGTGAGGCTCAACGAGGTTACTGTGGCAAAAAGAATAAGGTGCCATTGCTATCAGTTGAGGCATTCCATCACTTGCACATCTTTATCTTTGTCCTAGCCATTGTCCATGTGAGCTTCTGTGCTCTCACTATTGTCTTCGGAAGTTTAAAGATACATCAATGGAAAAAATGGGAGGATGAAAATGCAAAAGAGATTATTGAAGTAGATGAAGCTCAAACAAAAATTACCCATGTTCAGGAGCATGACTTTGTCAAGGATCACTTTTGGGGTATCGGTAAAAATTATGTTTTCATGGGATGGCTGCATTCTTTTCTCAACCAACTTTATGGATCAGTGTGCAGATCAGATTACTTGACTTTGCGATTGGGTTTCATCTCGACCCATTGCAGGGGAAACCCTAAGTTTAATTTCTACACATACATGGTACGCGCACTTGAAGATGATTTTAAAAGAGTCGTCGGTATCAGTTGGTATTTGTGGATATTTGTCGTCCTCTTCATGCTTCTGAATGTTGATGGATGGCATGCATACTTCTGGATTGCTTTTGTTCCTTTCTTTCTTCTACTTGCTGTCGGAACCAAGCTGCAACACGTAATCACCCAGTTGGCTCACGAGGTTGCTGAAAAACATATCGCCATAGAAGGGGAACTAATAGTTCAGCCATCAGACAAGCACTTTTGGTTTGGCAAGCCCAAGTTTGTTCTCTTCTTGATCCATTTCATCCTCTTCCAAAATGCTTTCGAAATGGCGTTCTTTTTCTGGATATGGGTTCAATATGGTTTTACATCTTGCATAATGGGGAAAGTAAAATACATTGTCCCCAGGCTTGTTATTGGGGTGTTCATTCAGGTGCTCTGTAGTTATAGTACTCTACCACTTTATGCGATTGTCACGCAGATGGGAACCAATTTCAAGAAAGCAATGTTCGATGAGAATATACGCTCAGGACTTGTTGGTTGGGCTCAAATGGCAAAGAGAAGGAAGGCGAGCATCGACGCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

467

Amino Acids

53.48

Weight (kDa)

9.36

Isoelectric Point (pI)

27.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 461 3.2e-185 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015815)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08130 FvH4_7g08130
malus_domestica MD02G1230200.v1.1 MD07G1082000.v1.1
prunus_persica Prupe.2G107600_v2.0.a1 Prupe.2G107600_v2.0.a1
pyrus_communis pycom07g06530
rosa_chinensis RchiOBHm_Chr1g0341101
rosa_laevigata RLG00000029064
rosa_multiflora Rmu_sc0001211.1_g000067
rosa_rugosa Rorug01G0159900.1
rosa_samantha Rh1AG174200 Rh1BG142500 Rh1CG162000
rosa_wichuraiana Rw1G014530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 436
AccII CGCG 1 cut(s) 825
AciI CCGC 4 cut(s) 56, 59, 117, 149
AclWI GGATC 4 cut(s) 242, 660, 733, 1102
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 4 cut(s) 125, 155, 822, 1264
AfiI CCNNNNNNNGG 4 cut(s) 631, 783, 784, 1038
AflIII ACRYGT 1 cut(s) 985
AgsI TTSAA 5 cut(s) 130, 596, 833, 1169, 1309
AjnI CCWGG 2 cut(s) 374, 1217
AluBI AGCT 5 cut(s) 184, 389, 510, 608, 979
AluI AGCT 5 cut(s) 184, 389, 510, 608, 979
Alw21I GWGCWC 4 cut(s) 68, 106, 520, 1251
Alw44I GTGCAC 2 cut(s) 64, 102
AlwI GGATC 4 cut(s) 242, 660, 733, 1102
ApaLI GTGCAC 2 cut(s) 64, 102
ApeKI GCWGC 6 cut(s) 190, 277, 382, 389, 697, 979
ApoI RAATTY 1 cut(s) 243
AspA2I CCTAGG 1 cut(s) 257
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 2 cut(s) 20, 368
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 3 cut(s) 184, 410, 985
AsuII TTCGAA 1 cut(s) 1137
AsuNHI GCTAGC 1 cut(s) 385
AvaII GGWCC 2 cut(s) 20, 368
AvrII CCTAGG 1 cut(s) 257
BaeGI GKGCMC 2 cut(s) 68, 106
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 2 cut(s) 257, 1360
BarI GAAGNNNNNNTAC 2 cut(s) 137, 169
BauI CACGAG 1 cut(s) 1007
BbsI GAAGAC 1 cut(s) 523
Bbv12I GWGCWC 4 cut(s) 68, 106, 520, 1251
BbvI GCAGC 6 cut(s) 177, 264, 369, 376, 684, 966
BccI CCATC 6 cut(s) 471, 687, 905, 909, 1066, 1288
BceAI ACGGC 1 cut(s) 331
BcgI CGANNNNNNTGC 4 cut(s) 55, 89, 308, 342
BciT130I CCWGG 2 cut(s) 376, 1219
BcnI CCSGG 1 cut(s) 394
BfaI CTAG 3 cut(s) 258, 386, 491
BfmI CTRYAG 2 cut(s) 29, 1252
BisI GCNGC 9 cut(s) 57, 118, 149, 191, 278, 383, 390, 698, 980
BlnI CCTAGG 1 cut(s) 257
BlsI GCNGC 9 cut(s) 58, 119, 150, 192, 279, 384, 391, 699, 981
BmcAI AGTACT 2 cut(s) 125, 1264
Bme1390I CCNGG 3 cut(s) 376, 394, 1219
Bme18I GGWCC 2 cut(s) 20, 368
BmgT120I GGNCC 2 cut(s) 20, 368
BmiI GGNNCC 3 cut(s) 438, 973, 1300
BmrFI CCNGG 3 cut(s) 376, 394, 1219
BmrI ACTGGG 1 cut(s) 991
BmsI GCATC 2 cut(s) 177, 1395
BmtI GCTAGC 1 cut(s) 389
BmuI ACTGGG 1 cut(s) 991
BpiI GAAGAC 1 cut(s) 523
Bpu10I CCTNAGC 1 cut(s) 1339
Bpu14I TTCGAA 1 cut(s) 1137
BpuMI CCSGG 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 988
BsaJI CCNNGG 3 cut(s) 257, 393, 1217
Bsc4I CCNNNNNNNGG 4 cut(s) 631, 783, 784, 1038
Bse1I ACTGG 1 cut(s) 997
Bse3DI GCAATG 3 cut(s) 440, 778, 1323
BseBI CCWGG 2 cut(s) 376, 1219
BseDI CCNNGG 3 cut(s) 257, 393, 1217
BseGI GGATG 4 cut(s) 577, 698, 920, 1116
BseLI CCNNNNNNNGG 4 cut(s) 631, 783, 784, 1038
BseMI GCAATG 3 cut(s) 440, 778, 1323
BseMII CTCAG 2 cut(s) 351, 1353
BseNI ACTGG 1 cut(s) 997
BseRI GAGGAG 3 cut(s) 194, 200, 203
BseSI GKGCMC 2 cut(s) 68, 106
BseXI GCAGC 6 cut(s) 177, 264, 369, 376, 684, 966
BseYI CCCAGC 1 cut(s) 193
BsgI GTGCAG 1 cut(s) 754
Bsh1236I CGCG 1 cut(s) 825
BshNI GGYRCC 1 cut(s) 436
BsiHKAI GWGCWC 4 cut(s) 68, 106, 520, 1251
BsiSI CCGG 2 cut(s) 114, 393
BslFI GGGAC 1 cut(s) 1199
BslI CCNNNNNNNGG 4 cut(s) 631, 783, 784, 1038
BsmFI GGGAC 1 cut(s) 1199
BsmI GAATGC 3 cut(s) 360, 458, 700
Bsp119I TTCGAA 1 cut(s) 1137
Bsp1286I GDGCHC 6 cut(s) 68, 106, 257, 520, 1251, 1360
Bsp143I GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
BspACI CCGC 4 cut(s) 56, 59, 117, 149
BspCNI CTCAG 2 cut(s) 350, 1352
BspFNI CGCG 1 cut(s) 825
BspLI GGNNCC 3 cut(s) 438, 973, 1300
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 4 cut(s) 242, 660, 733, 1102
BspT104I TTCGAA 1 cut(s) 1137
BspT107I GGYRCC 1 cut(s) 436
BsrDI GCAATG 3 cut(s) 440, 778, 1323
BsrI ACTGG 1 cut(s) 997
BssECI CCNNGG 3 cut(s) 257, 393, 1217
BssMI GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
BssSI CACGAG 1 cut(s) 1007
BssT1I CCWWGG 1 cut(s) 257
Bst2BI CACGAG 1 cut(s) 1007
Bst2UI CCWGG 2 cut(s) 376, 1219
Bst4CI ACNGT 3 cut(s) 33, 220, 419
Bst6I CTCTTC 4 cut(s) 224, 896, 1106, 1127
BstBAI YACGTR 1 cut(s) 988
BstBI TTCGAA 1 cut(s) 1137
BstC8I GCNNGC 6 cut(s) 68, 102, 387, 921, 1085, 1384
BstDEI CTNAG 3 cut(s) 337, 795, 1339
BstF5I GGATG 4 cut(s) 577, 698, 920, 1116
BstFNI CGCG 1 cut(s) 825
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 7 cut(s) 75, 237, 307, 655, 728, 740, 1110
BstMBI GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
BstMWI GCNNNNNNNGC 3 cut(s) 148, 190, 1364
BstNI CCWGG 2 cut(s) 376, 1219
BstNSI RCATGY 1 cut(s) 923
BstSCI CCNGG 3 cut(s) 374, 392, 1217
BstSFI CTRYAG 2 cut(s) 29, 1252
BstSLI GKGCMC 2 cut(s) 68, 106
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 6 cut(s) 177, 264, 369, 376, 684, 966
BstV2I GAAGAC 1 cut(s) 523
BstXI CCANNNNNNTGG 1 cut(s) 722
BtsCI GGATG 4 cut(s) 577, 698, 920, 1116
BtsIMutI CAGTG 2 cut(s) 216, 735
Cac8I GCNNGC 6 cut(s) 68, 102, 387, 921, 1085, 1384
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 2 cut(s) 20, 368
Csp6I GTAC 4 cut(s) 124, 154, 821, 1263
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviAII CATG 9 cut(s) 45, 282, 503, 626, 638, 688, 817, 895, 920
CviQI GTAC 4 cut(s) 124, 154, 821, 1263
DdeI CTNAG 3 cut(s) 337, 795, 1339
DpnI GATC 7 cut(s) 74, 236, 306, 654, 727, 739, 1109
DpnII GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
DraI TTTAAA 2 cut(s) 543, 844
Eam1104I CTCTTC 4 cut(s) 224, 896, 1106, 1127
EarI CTCTTC 4 cut(s) 224, 896, 1106, 1127
Eco130I CCWWGG 1 cut(s) 257
Eco24I GRGCYC 2 cut(s) 257, 1360
Eco47I GGWCC 2 cut(s) 20, 368
Eco57I CTGAAG 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 374, 1217
EcoT14I CCWWGG 1 cut(s) 257
EcoT22I ATGCAT 1 cut(s) 925
EcoT38I GRGCYC 2 cut(s) 257, 1360
ErhI CCWWGG 1 cut(s) 257
FaeI CATG 9 cut(s) 48, 285, 506, 629, 641, 691, 820, 898, 923
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 1 cut(s) 1199
FatI CATG 9 cut(s) 44, 281, 502, 625, 637, 687, 816, 894, 919
Fnu4HI GCNGC 9 cut(s) 57, 118, 149, 191, 278, 383, 390, 698, 980
FokI GGATG 4 cut(s) 584, 705, 927, 1103
FriOI GRGCYC 2 cut(s) 257, 1360
Fsp4HI GCNGC 9 cut(s) 57, 118, 149, 191, 278, 383, 390, 698, 980
FspBI CTAG 3 cut(s) 258, 386, 491
GlaI GCGC 1 cut(s) 826
GluI GCNGC 9 cut(s) 57, 118, 149, 191, 278, 383, 390, 698, 980
GsaI CCCAGC 1 cut(s) 197
HapII CCGG 2 cut(s) 114, 393
HhaI GCGC 1 cut(s) 827
Hin1II CATG 9 cut(s) 48, 285, 506, 629, 641, 691, 820, 898, 923
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HinfI GANTC 1 cut(s) 849
HpaII CCGG 2 cut(s) 114, 393
HphI GGTGA 3 cut(s) 184, 410, 985
Hpy166II GTNNAC 3 cut(s) 36, 66, 104
Hpy188I TCNGA 7 cut(s) 228, 312, 536, 742, 903, 971, 1063
Hpy188III TCNNGA 8 cut(s) 632, 772, 931, 1007, 1105, 1156, 1309, 1341
Hpy8I GTNNAC 3 cut(s) 36, 66, 104
Hpy99I CGWCG 2 cut(s) 857, 1394
HpyAV CCTTC 6 cut(s) 11, 163, 169, 1031, 1368, 1372
HpyCH4III ACNGT 3 cut(s) 33, 220, 419
HpyCH4IV ACGT 1 cut(s) 987
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 190, 1364
HpyF3I CTNAG 3 cut(s) 337, 795, 1339
HpySE526I ACGT 1 cut(s) 987
Hsp92II CATG 9 cut(s) 48, 285, 506, 629, 641, 691, 820, 898, 923
HspAI GCGC 1 cut(s) 825
Kzo9I GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
LmnI GCTCC 3 cut(s) 97, 181, 634
Lsp1109I GCAGC 6 cut(s) 177, 264, 369, 376, 684, 966
LweI GCATC 2 cut(s) 177, 1395
MaeI CTAG 3 cut(s) 258, 386, 491
MaeII ACGT 1 cut(s) 987
MaeIII GTNAC 2 cut(s) 413, 1285
MalI GATC 7 cut(s) 74, 236, 306, 654, 727, 739, 1109
MboI GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
MhlI GDGCHC 6 cut(s) 68, 106, 257, 520, 1251, 1360
MluCI AATT 5 cut(s) 243, 618, 676, 802, 1303
MlyI GAGTC 1 cut(s) 858
MmeI TCCRAC 2 cut(s) 47, 949
Mph1103I ATGCAT 1 cut(s) 925
MseI TTAA 4 cut(s) 542, 801, 843, 1399
MslI CAYNNNNRTG 3 cut(s) 815, 1244, 1292
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 2 cut(s) 114, 393
MspR9I CCNGG 3 cut(s) 376, 394, 1219
Mva1269I GAATGC 3 cut(s) 360, 458, 700
MvaI CCWGG 2 cut(s) 376, 1219
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 3 cut(s) 148, 190, 1364
NciI CCSGG 1 cut(s) 394
NdeII GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
NheI GCTAGC 1 cut(s) 385
NlaIII CATG 9 cut(s) 48, 285, 506, 629, 641, 691, 820, 898, 923
NlaIV GGNNCC 3 cut(s) 438, 973, 1300
NmuCI GTSAC 1 cut(s) 1285
NsiI ATGCAT 1 cut(s) 925
NspI RCATGY 1 cut(s) 923
NspV TTCGAA 1 cut(s) 1137
PaeI GCATGC 1 cut(s) 923
PctI GAATGC 3 cut(s) 360, 458, 700
PflFI GACNNNGTC 1 cut(s) 644
PkrI GCNGC 9 cut(s) 58, 119, 150, 192, 279, 384, 391, 699, 981
PleI GAGTC 1 cut(s) 857
PpsI GAGTC 1 cut(s) 857
Ppu21I YACGTR 1 cut(s) 988
Psp6I CCWGG 2 cut(s) 374, 1217
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 374, 1217
PspN4I GGNNCC 3 cut(s) 438, 973, 1300
PspPI GGNCC 2 cut(s) 20, 368
PsyI GACNNNGTC 1 cut(s) 644
RsaI GTAC 4 cut(s) 125, 155, 822, 1264
RsaNI GTAC 4 cut(s) 124, 154, 821, 1263
RseI CAYNNNNRTG 3 cut(s) 815, 1244, 1292
SaqAI TTAA 4 cut(s) 542, 801, 843, 1399
SatI GCNGC 9 cut(s) 57, 118, 149, 191, 278, 383, 390, 698, 980
Sau3AI GATC 7 cut(s) 72, 234, 304, 652, 725, 737, 1107
Sau96I GGNCC 2 cut(s) 20, 368
ScaI AGTACT 2 cut(s) 125, 1264
SchI GAGTC 1 cut(s) 858
ScrFI CCNGG 3 cut(s) 376, 394, 1219
SduI GDGCHC 6 cut(s) 68, 106, 257, 520, 1251, 1360
SfaNI GCATC 2 cut(s) 177, 1395
SfcI CTRYAG 2 cut(s) 29, 1252
SfuI TTCGAA 1 cut(s) 1137
SinI GGWCC 2 cut(s) 20, 368
SmiMI CAYNNNNRTG 3 cut(s) 815, 1244, 1292
SphI GCATGC 1 cut(s) 923
Sse9I AATT 5 cut(s) 243, 618, 676, 802, 1303
SsiI CCGC 4 cut(s) 56, 59, 117, 149
SspMI CTAG 3 cut(s) 258, 386, 491
StyD4I CCNGG 3 cut(s) 374, 392, 1217
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 3 cut(s) 33, 220, 419
TaiI ACGT 1 cut(s) 990
TaqI TCGA 4 cut(s) 773, 1137, 1323, 1389
TasI AATT 5 cut(s) 243, 618, 676, 802, 1303
TatI WGTACW 2 cut(s) 123, 1262
TauI GCSGC 3 cut(s) 59, 120, 151
Tru1I TTAA 4 cut(s) 542, 801, 843, 1399
Tru9I TTAA 4 cut(s) 542, 801, 843, 1399
TscAI CASTG 2 cut(s) 223, 735
TseFI GTSAC 1 cut(s) 1285
TseI GCWGC 6 cut(s) 190, 277, 382, 389, 697, 979
Tsp45I GTSAC 1 cut(s) 1285
TspDTI ATGAA 8 cut(s) 190, 588, 618, 676, 757, 883, 1105, 1228
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 2 cut(s) 223, 735
Tth111I GACNNNGTC 1 cut(s) 644
VneI GTGCAC 2 cut(s) 64, 102
VpaK11BI GGWCC 2 cut(s) 20, 368
XapI RAATTY 1 cut(s) 243
XceI RCATGY 1 cut(s) 923
XmaJI CCTAGG 1 cut(s) 257
XspI CTAG 3 cut(s) 258, 386, 491
ZrmI AGTACT 2 cut(s) 125, 1264
Zsp2I ATGCAT 1 cut(s) 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.