pycom07g06530

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015815
Physical Location & Seq
Forward (+)
5836924 .. 5840963
4040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06530.1

Sequence Viewer

Length: 1329 bp
ATGAGTAGAAAAAAAGGTGAAAAAATGGCTGGTGGGGGAGAAGGTCCAACGCTAGAGTTCACGCCAACATGGGTGGTGGCGGTGGTCTGCACGGTGATCGTGGCTATATCTCTCGCAGTAGAGCGTGCCCTACACTCCGGCGGCAAGTACTTGAAAAAGAAGAACCAGAAGCCGCTGTACGAGGCGTTGCTCAAGGTGAAGGAGGAGCTGATGCTGCTGGGGTTTATCTCTCTCCTCCTCACTGTCTTTCAGAACAGGATCACCAAAATCTGCGTGAGCTCTAAGATTATGGACAAGTGGCTGCCATGCAAACTCCACCATGACGGTCATCTGGACGGCAAAGAAACTGCACATTACTCCGCCACCTCCGTGTTTGGCGGCATTTTTGGACCTACCAGGAGGCTGCTGGCTGCCGGGGCGAAGGCTCAAGAGGGTTACTGTGGCAAAAAGAATAAGGTACCATTGCTATCTGTTGAGGCATTCCATCACCTGCACATCTTTATCTTTGTCCTAGCCATTGTCCATGTGACCTTCTGTGCTCTCACAATTGTCTTCGGAGGTTTAAAGATACGTCAATGGAAACAATGGGAGGAGGCAAATGCAAAAGAGATTAGCGAAGTAGATGAAGCTCAAACAAAGATCACCCATGTTCAGGAGCATGACTTTGTCAAGGATCACTTTTGGGGTATCGGCAAAAATTATCGTGTGAAGGGATGGCTGCATTCTTTCCTCAACCAGTTTTATGGATCAGTGTGCAGATCAGACTACTTGACTTTGCGACTTGGTTTCATCTCGACCCATTGCAGGGGAAACCCCAAGTTTGATTTCTACACGTACATGGTACGCACCCTTGAAGATGATTTTAAAAGAGTCGTCGGTATCAGTTGGTATCTTTGGATATTTGTGGTCATCTTCATGCTTATGAATGTCGATGGATGGCATGCATACTTCTGGATTGCATTTATTCCTTTCTTTCTTTTGCTTGCTGTTGGAACTAAGCTGCAGCACGTAATCACCCAGTTGGCTCACGAGGTTGCTGAGAAGCATATCGCCGTAGAAGGAGAACTAATAGTTCAACCATCAGACAAGCACTTTTGGTTTGGCAAGCCCAAATTTGTTCTCTTCTTGATTCATCTCATCCTCTTCCAAAATGCTTTCGAAATGGCATTCTTTTTCTGGATATGGGTTCAATATGGTTTTACATCTTGCATAATGGGAAAAGTAAGATACATTGTCCCCAGGCTTGTTATTGGGGTGTTCATTCAGGTGCTCTGCAGTTACAGTACTCTACCACTTTATGCCATTGTCACGCAGGTGCGTCGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

443

Amino Acids

50.79

Weight (kDa)

9.34

Isoelectric Point (pI)

23.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 17 - 439 8.7e-176 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015815)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08130 FvH4_7g08130
malus_domestica MD02G1230200.v1.1 MD07G1082000.v1.1
prunus_persica Prupe.2G107600_v2.0.a1 Prupe.2G107600_v2.0.a1
pyrus_communis pycom07g06530
rosa_chinensis RchiOBHm_Chr1g0341101
rosa_laevigata RLG00000029064
rosa_multiflora Rmu_sc0001211.1_g000067
rosa_rugosa Rorug01G0159900.1
rosa_samantha Rh1AG174200 Rh1BG142500 Rh1CG162000
rosa_wichuraiana Rw1G014530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 498, 1303
Acc36I ACCTGC 2 cut(s) 498, 1303
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 1 cut(s) 457
AciI CCGC 5 cut(s) 80, 141, 173, 360, 378
AclWI GGATC 3 cut(s) 266, 681, 754
AcsI RAATTY 1 cut(s) 1112
AfaI GTAC 6 cut(s) 149, 179, 459, 836, 843, 1285
AfiI CCNNNNNNNGG 3 cut(s) 652, 804, 805
AflIII ACRYGT 1 cut(s) 831
AgsI TTSAA 4 cut(s) 154, 854, 1076, 1190
AjnI CCWGG 2 cut(s) 395, 1238
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AleI CACNNNNGTG 2 cut(s) 368, 1313
AluBI AGCT 4 cut(s) 208, 279, 629, 1000
AluI AGCT 4 cut(s) 208, 279, 629, 1000
Alw21I GWGCWC 3 cut(s) 281, 541, 1272
AlwI GGATC 3 cut(s) 266, 681, 754
ApeKI GCWGC 7 cut(s) 214, 301, 403, 410, 718, 1000, 1003
ApoI RAATTY 1 cut(s) 1112
Asp718I GGTACC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 44, 389
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 7 cut(s) 29, 106, 208, 253, 479, 634, 1006
AsuII TTCGAA 1 cut(s) 1158
AvaII GGWCC 2 cut(s) 44, 389
BaeGI GKGCMC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 281
BarI GAAGNNNNNNTAC 2 cut(s) 161, 193
BauI CACGAG 1 cut(s) 1028
BbsI GAAGAC 1 cut(s) 544
Bbv12I GWGCWC 3 cut(s) 281, 541, 1272
BbvI GCAGC 7 cut(s) 201, 288, 390, 397, 705, 987, 1015
BccI CCATC 5 cut(s) 492, 708, 926, 930, 1087
BceAI ACGGC 2 cut(s) 352, 1037
BcgI CGANNNNNNTGC 3 cut(s) 79, 113, 1301
BciT130I CCWGG 2 cut(s) 397, 1240
BcnI CCSGG 1 cut(s) 415
BfaI CTAG 2 cut(s) 53, 512
BfmI CTRYAG 2 cut(s) 1001, 1273
BfuAI ACCTGC 2 cut(s) 498, 1303
BmcAI AGTACT 2 cut(s) 149, 1285
Bme1390I CCNGG 3 cut(s) 397, 415, 1240
Bme18I GGWCC 2 cut(s) 44, 389
BmgT120I GGNCC 2 cut(s) 44, 389
BmiI GGNNCC 1 cut(s) 459
BmrFI CCNGG 3 cut(s) 397, 415, 1240
BmrI ACTGGG 1 cut(s) 1012
BmsI GCATC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 1012
BpiI GAAGAC 1 cut(s) 544
Bpu14I TTCGAA 1 cut(s) 1158
BpuEI CTTGAG 2 cut(s) 176, 411
BpuMI CCSGG 1 cut(s) 415
BsaAI YACGTR 2 cut(s) 834, 1009
BsaJI CCNNGG 2 cut(s) 414, 1238
Bsc4I CCNNNNNNNGG 3 cut(s) 652, 804, 805
Bse1I ACTGG 2 cut(s) 736, 1018
Bse3DI GCAATG 2 cut(s) 461, 799
BseBI CCWGG 2 cut(s) 397, 1240
BseDI CCNNGG 2 cut(s) 414, 1238
BseGI GGATG 3 cut(s) 719, 941, 1137
BseLI CCNNNNNNNGG 3 cut(s) 652, 804, 805
BseMI GCAATG 2 cut(s) 461, 799
BseMII CTCAG 1 cut(s) 1029
BseNI ACTGG 2 cut(s) 736, 1018
BseRI GAGGAG 4 cut(s) 218, 224, 227, 605
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 7 cut(s) 201, 288, 390, 397, 705, 987, 1015
BseYI CCCAGC 1 cut(s) 217
BsgI GTGCAG 4 cut(s) 73, 333, 476, 775
BshNI GGYRCC 1 cut(s) 457
BsiHKAI GWGCWC 3 cut(s) 281, 541, 1272
BsiSI CCGG 2 cut(s) 138, 414
BslFI GGGAC 1 cut(s) 1220
BslI CCNNNNNNNGG 3 cut(s) 652, 804, 805
BsmFI GGGAC 1 cut(s) 1220
BsmI GAATGC 3 cut(s) 479, 721, 1166
Bsp119I TTCGAA 1 cut(s) 1158
Bsp1286I GDGCHC 4 cut(s) 130, 281, 541, 1272
Bsp143I GATC 6 cut(s) 96, 258, 639, 673, 746, 758
BspACI CCGC 5 cut(s) 80, 141, 173, 360, 378
BspCNI CTCAG 1 cut(s) 1030
BspLI GGNNCC 1 cut(s) 459
BspMAI CTGCAG 2 cut(s) 1005, 1277
BspMI ACCTGC 2 cut(s) 498, 1303
BspPI GGATC 3 cut(s) 266, 681, 754
BspT104I TTCGAA 1 cut(s) 1158
BspT107I GGYRCC 1 cut(s) 457
BsrDI GCAATG 2 cut(s) 461, 799
BsrI ACTGG 2 cut(s) 736, 1018
BssECI CCNNGG 2 cut(s) 414, 1238
BssMI GATC 6 cut(s) 96, 258, 639, 673, 746, 758
BssSI CACGAG 1 cut(s) 1028
Bst2BI CACGAG 1 cut(s) 1028
Bst2UI CCWGG 2 cut(s) 397, 1240
Bst4CI ACNGT 5 cut(s) 94, 244, 326, 440, 1283
Bst6I CTCTTC 2 cut(s) 1127, 1148
BstBAI YACGTR 2 cut(s) 834, 1009
BstBI TTCGAA 1 cut(s) 1158
BstC8I GCNNGC 5 cut(s) 126, 408, 942, 984, 1106
BstDEI CTNAG 3 cut(s) 282, 996, 1038
BstF5I GGATG 3 cut(s) 719, 941, 1137
BstKTI GATC 6 cut(s) 99, 261, 642, 676, 749, 761
BstMBI GATC 6 cut(s) 96, 258, 639, 673, 746, 758
BstMWI GCNNNNNNNGC 2 cut(s) 214, 416
BstNI CCWGG 2 cut(s) 397, 1240
BstNSI RCATGY 1 cut(s) 944
BstSCI CCNGG 3 cut(s) 395, 413, 1238
BstSFI CTRYAG 2 cut(s) 1001, 1273
BstSLI GKGCMC 1 cut(s) 130
BstV1I GCAGC 7 cut(s) 201, 288, 390, 397, 705, 987, 1015
BstV2I GAAGAC 1 cut(s) 544
BstXI CCANNNNNNTGG 1 cut(s) 743
BtsCI GGATG 3 cut(s) 719, 941, 1137
BtsIMutI CAGTG 2 cut(s) 240, 756
BveI ACCTGC 2 cut(s) 498, 1303
Cac8I GCNNGC 5 cut(s) 126, 408, 942, 984, 1106
Cfr13I GGNCC 2 cut(s) 44, 389
CseI GACGC 1 cut(s) 1307
Csp6I GTAC 6 cut(s) 148, 178, 458, 835, 842, 1284
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviAII CATG 9 cut(s) 69, 306, 320, 524, 647, 659, 838, 916, 941
CviQI GTAC 6 cut(s) 148, 178, 458, 835, 842, 1284
DdeI CTNAG 3 cut(s) 282, 996, 1038
DpnI GATC 6 cut(s) 98, 260, 641, 675, 748, 760
DpnII GATC 6 cut(s) 96, 258, 639, 673, 746, 758
DraI TTTAAA 2 cut(s) 564, 865
Eam1104I CTCTTC 2 cut(s) 1127, 1148
EarI CTCTTC 2 cut(s) 1127, 1148
EciI GGCGGA 1 cut(s) 349
Ecl136II GAGCTC 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 281
Eco47I GGWCC 2 cut(s) 44, 389
Eco53kI GAGCTC 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 395, 1238
EcoT22I ATGCAT 1 cut(s) 946
EcoT38I GRGCYC 1 cut(s) 281
FaeI CATG 9 cut(s) 72, 309, 323, 527, 650, 662, 841, 919, 944
FalI AAGNNNNNCTT 2 cut(s) 662, 694
FaqI GGGAC 1 cut(s) 1220
FatI CATG 9 cut(s) 68, 305, 319, 523, 646, 658, 837, 915, 940
FokI GGATG 3 cut(s) 726, 948, 1124
FriOI GRGCYC 1 cut(s) 281
FspBI CTAG 2 cut(s) 53, 512
GsaI CCCAGC 1 cut(s) 221
HapII CCGG 2 cut(s) 138, 414
HgaI GACGC 1 cut(s) 1307
Hin1II CATG 9 cut(s) 72, 309, 323, 527, 650, 662, 841, 919, 944
HinfI GANTC 2 cut(s) 870, 1129
HpaII CCGG 2 cut(s) 138, 414
HphI GGTGA 7 cut(s) 29, 106, 208, 253, 479, 634, 1006
Hpy166II GTNNAC 1 cut(s) 60
Hpy188I TCNGA 4 cut(s) 252, 557, 763, 1084
Hpy188III TCNNGA 8 cut(s) 332, 428, 653, 793, 952, 1028, 1126, 1177
Hpy8I GTNNAC 1 cut(s) 60
Hpy99I CGWCG 2 cut(s) 878, 1323
HpyAV CCTTC 6 cut(s) 35, 193, 415, 541, 703, 1052
HpyCH4III ACNGT 5 cut(s) 94, 244, 326, 440, 1283
HpyCH4IV ACGT 3 cut(s) 571, 833, 1008
HpyF10VI GCNNNNNNNGC 2 cut(s) 214, 416
HpyF3I CTNAG 3 cut(s) 282, 996, 1038
HpySE526I ACGT 3 cut(s) 571, 833, 1008
Hsp92II CATG 9 cut(s) 72, 309, 323, 527, 650, 662, 841, 919, 944
KpnI GGTACC 1 cut(s) 461
Kzo9I GATC 6 cut(s) 96, 258, 639, 673, 746, 758
LmnI GCTCC 2 cut(s) 205, 655
Lsp1109I GCAGC 7 cut(s) 201, 288, 390, 397, 705, 987, 1015
LweI GCATC 1 cut(s) 201
MaeI CTAG 2 cut(s) 53, 512
MaeII ACGT 3 cut(s) 571, 833, 1008
MaeIII GTNAC 4 cut(s) 434, 526, 1277, 1306
MalI GATC 6 cut(s) 98, 260, 641, 675, 748, 760
MboI GATC 6 cut(s) 96, 258, 639, 673, 746, 758
MboII GAAGA 6 cut(s) 172, 544, 866, 904, 1114, 1135
MfeI CAATTG 1 cut(s) 546
MhlI GDGCHC 4 cut(s) 130, 281, 541, 1272
MluCI AATT 3 cut(s) 546, 697, 1112
MlyI GAGTC 1 cut(s) 879
MmeI TCCRAC 2 cut(s) 71, 970
Mph1103I ATGCAT 1 cut(s) 946
MseI TTAA 3 cut(s) 563, 864, 1327
MslI CAYNNNNRTG 6 cut(s) 368, 836, 914, 920, 1265, 1313
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 2 cut(s) 138, 414
MspR9I CCNGG 3 cut(s) 397, 415, 1240
MunI CAATTG 1 cut(s) 546
Mva1269I GAATGC 3 cut(s) 479, 721, 1166
MvaI CCWGG 2 cut(s) 397, 1240
MwoI GCNNNNNNNGC 2 cut(s) 214, 416
NciI CCSGG 1 cut(s) 415
NdeII GATC 6 cut(s) 96, 258, 639, 673, 746, 758
NlaIII CATG 9 cut(s) 72, 309, 323, 527, 650, 662, 841, 919, 944
NlaIV GGNNCC 1 cut(s) 459
NmuCI GTSAC 2 cut(s) 526, 1306
NsiI ATGCAT 1 cut(s) 946
NspI RCATGY 1 cut(s) 944
NspV TTCGAA 1 cut(s) 1158
OliI CACNNNNGTG 2 cut(s) 368, 1313
PaeI GCATGC 1 cut(s) 944
PaqCI CACCTGC 2 cut(s) 498, 1303
PctI GAATGC 3 cut(s) 479, 721, 1166
PfeI GAWTC 1 cut(s) 1129
PflFI GACNNNGTC 1 cut(s) 665
PleI GAGTC 1 cut(s) 878
PpsI GAGTC 1 cut(s) 878
Ppu21I YACGTR 2 cut(s) 834, 1009
Psp124BI GAGCTC 1 cut(s) 281
Psp6I CCWGG 2 cut(s) 395, 1238
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 2 cut(s) 395, 1238
PspN4I GGNNCC 1 cut(s) 459
PspPI GGNCC 2 cut(s) 44, 389
PstI CTGCAG 2 cut(s) 1005, 1277
PsyI GACNNNGTC 1 cut(s) 665
RsaI GTAC 6 cut(s) 149, 179, 459, 836, 843, 1285
RsaNI GTAC 6 cut(s) 148, 178, 458, 835, 842, 1284
RseI CAYNNNNRTG 6 cut(s) 368, 836, 914, 920, 1265, 1313
SacI GAGCTC 1 cut(s) 281
SaqAI TTAA 3 cut(s) 563, 864, 1327
Sau3AI GATC 6 cut(s) 96, 258, 639, 673, 746, 758
Sau96I GGNCC 2 cut(s) 44, 389
ScaI AGTACT 2 cut(s) 149, 1285
SchI GAGTC 1 cut(s) 879
ScrFI CCNGG 3 cut(s) 397, 415, 1240
SduI GDGCHC 4 cut(s) 130, 281, 541, 1272
SfaNI GCATC 1 cut(s) 201
SfcI CTRYAG 2 cut(s) 1001, 1273
SfuI TTCGAA 1 cut(s) 1158
SinI GGWCC 2 cut(s) 44, 389
SmiMI CAYNNNNRTG 6 cut(s) 368, 836, 914, 920, 1265, 1313
SmlI CTYRAG 2 cut(s) 191, 426
SmoI CTYRAG 2 cut(s) 191, 426
SphI GCATGC 1 cut(s) 944
Sse9I AATT 3 cut(s) 546, 697, 1112
SsiI CCGC 5 cut(s) 80, 141, 173, 360, 378
SspMI CTAG 2 cut(s) 53, 512
SstI GAGCTC 1 cut(s) 281
StyD4I CCNGG 3 cut(s) 395, 413, 1238
TaaI ACNGT 5 cut(s) 94, 244, 326, 440, 1283
TaiI ACGT 3 cut(s) 574, 836, 1011
TaqI TCGA 4 cut(s) 794, 930, 1158, 1321
TasI AATT 3 cut(s) 546, 697, 1112
TatI WGTACW 2 cut(s) 147, 1283
TauI GCSGC 3 cut(s) 144, 175, 381
TfiI GAWTC 1 cut(s) 1129
Tru1I TTAA 3 cut(s) 563, 864, 1327
Tru9I TTAA 3 cut(s) 563, 864, 1327
TscAI CASTG 2 cut(s) 247, 756
TseFI GTSAC 2 cut(s) 526, 1306
TseI GCWGC 7 cut(s) 214, 301, 403, 410, 718, 1000, 1003
Tsp45I GTSAC 2 cut(s) 526, 1306
TspDTI ATGAA 6 cut(s) 639, 778, 904, 938, 1121, 1249
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 2 cut(s) 247, 756
Tth111I GACNNNGTC 1 cut(s) 665
VpaK11BI GGWCC 2 cut(s) 44, 389
XapI RAATTY 1 cut(s) 1112
XceI RCATGY 1 cut(s) 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XspI CTAG 2 cut(s) 53, 512
ZrmI AGTACT 2 cut(s) 149, 1285
Zsp2I ATGCAT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.