Rh1AG174200

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0015815
Physical Location & Seq
Forward (+)
33420838 .. 33429717
8880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG174200.1

Sequence Viewer

Length: 1011 bp
ATGGCTGGTGGAGGCGAAGGTGTGACGCTAGAGTTCACTCCAACATGGGTGGTCGCACTAGTATGCTTCATCATAGTCAGCATCTCTCTTTTGGCAGAGAGAGGCCTTCACTCTGCTGGGAAGTACTTCAAGAAGAAGAACCAGAATCCGCTGTATGAGGCATTGCTGAAAGTGAAGGAGGAGCTCATGTTGTTGGGCTTTATCTCTCTTCTACTGACTGTGTCCCAGAAAACCATCACCAAAATGTGCGTCAAAGAGTCATTATATAAGTCGTGGCAGCCATGCAGACCAGATCCGGGTCCCCATAATAGGCCCAAAAACAGTACCCATTTTGAGCTAGAAAGTATTTCTGGGAATTTAAGGAGGCTTCTGGCTGCTGGGGGTGAGGCTCAAGAGGGGTACTGTGCCCAAAAGGGTAAGGTACCATTGTTATCAGTTCAAGCTTTCCATCATCTGCATATCTTTATATTTGTCCTAGCCATCGCTCATGTGACCTTCTCTGCTATGACTATTTGTTTCGGTGGGCTAAAGATACGCCAATGGAAACAGTGGGAGGAGTTGGTTGCAAAAGAGATTGATGATACAGAATCTGCTAAACCAAAGGTCACCCATGTTCAAGACCATGATTTTGTCAAAGATCATTATTTGGGTATTGGTAAAAATTATCGAATCAAGGGTTGGATACATTCTTTTAAAAACCAATTTTATGGATCTGTGACCAAATCAGATTATTTGACTTTGCGTCTAGGCTTCATCTCGACTCATTGCAAGGAAAATCCCAAGTTTGATTTTCACGATTACATGGTGCGTGCGCTTGAAAATGATTTCAAAAAAGTTGTCGGTATCAGTTGGTATCTATGGTTATTCGTAGTCATCTTTCTGCTGATCAATGTTGATGGTTTGCATGCATACTTCTGGCTAGCGTTCATCCCTTTCTTTGTGAGTACTCTAGCTCTTAGTTGGCATGATCCATTTTGTTCATATATGCATTTTATTGCATTTAATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

336

Amino Acids

38.53

Weight (kDa)

8.91

Isoelectric Point (pI)

24.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 314 1.4e-115 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015815)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08130 FvH4_7g08130
malus_domestica MD02G1230200.v1.1 MD07G1082000.v1.1
prunus_persica Prupe.2G107600_v2.0.a1 Prupe.2G107600_v2.0.a1
pyrus_communis pycom07g06530
rosa_chinensis RchiOBHm_Chr1g0341101
rosa_laevigata RLG00000029064
rosa_multiflora Rmu_sc0001211.1_g000067
rosa_rugosa Rorug01G0159900.1
rosa_samantha Rh1AG174200 Rh1BG142500 Rh1CG162000
rosa_wichuraiana Rw1G014530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 421
AccB1I GGYRCC 1 cut(s) 421
AciI CCGC 1 cut(s) 149
AclWI GGATC 3 cut(s) 287, 718, 962
AcsI RAATTY 1 cut(s) 355
AfaI GTAC 5 cut(s) 125, 325, 401, 423, 946
AfiI CCNNNNNNNGG 2 cut(s) 296, 309
AgsI TTSAA 5 cut(s) 130, 440, 617, 818, 829
AhdI GACNNNNNGTC 1 cut(s) 741
AhlI ACTAGT 1 cut(s) 58
AjuI GAANNNNNNNTTGG 2 cut(s) 661, 693
AluBI AGCT 4 cut(s) 184, 337, 443, 953
AluI AGCT 4 cut(s) 184, 337, 443, 953
Alw21I GWGCWC 1 cut(s) 186
AlwI GGATC 3 cut(s) 287, 718, 962
AlwNI CAGNNNCTG 1 cut(s) 590
AoxI GGCC 2 cut(s) 103, 311
ApeKI GCWGC 2 cut(s) 277, 374
ApoI RAATTY 1 cut(s) 355
ArsI GACNNNNNNTTYG 6 cut(s) 234, 266, 611, 643, 822, 854
Asp700I GAANNNNTTC 1 cut(s) 125
Asp718I GGTACC 1 cut(s) 421
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 2 cut(s) 299, 312
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 3 cut(s) 229, 395, 598
AsuNHI GCTAGC 1 cut(s) 919
AvaII GGWCC 1 cut(s) 299
BaeGI GKGCMC 1 cut(s) 409
BaeI ACNNNNGTAYC 2 cut(s) 413, 446
BanI GGYRCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 289, 361
BccI CCATC 4 cut(s) 242, 456, 488, 890
BciVI GTATCC 1 cut(s) 675
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 1 cut(s) 297
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 7 cut(s) 29, 59, 338, 476, 746, 920, 950
BfuI GTATCC 1 cut(s) 675
BisI GCNGC 2 cut(s) 278, 375
BlsI GCNGC 2 cut(s) 279, 376
BmcAI AGTACT 2 cut(s) 125, 946
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 299
BmeRI GACNNNNNGTC 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 299, 312
BmiI GGNNCC 3 cut(s) 300, 301, 423
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 90
BmtI GCTAGC 1 cut(s) 923
BpuEI CTTGAG 1 cut(s) 375
BpuMI CCSGG 1 cut(s) 297
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 309
Bse3DI GCAATG 2 cut(s) 161, 763
BseGI GGATG 1 cut(s) 927
BseLI CCNNNNNNNGG 2 cut(s) 296, 309
BseMI GCAATG 2 cut(s) 161, 763
BseRI GAGGAG 2 cut(s) 194, 569
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 2 cut(s) 289, 361
BseYI CCCAGC 2 cut(s) 116, 377
BshFI GGCC 2 cut(s) 105, 313
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 2 cut(s) 208, 285
BslI CCNNNNNNNGG 2 cut(s) 296, 309
BsmFI GGGAC 2 cut(s) 208, 285
BsnI GGCC 2 cut(s) 105, 313
Bsp1286I GDGCHC 2 cut(s) 186, 409
Bsp143I GATC 5 cut(s) 292, 637, 710, 885, 967
BspACI CCGC 1 cut(s) 149
BspANI GGCC 2 cut(s) 105, 313
BspLI GGNNCC 3 cut(s) 300, 301, 423
BspOI GCTAGC 1 cut(s) 923
BspPI GGATC 3 cut(s) 287, 718, 962
BspT107I GGYRCC 1 cut(s) 421
BsrDI GCAATG 2 cut(s) 161, 763
BssMI GATC 5 cut(s) 292, 637, 710, 885, 967
Bst4CI ACNGT 4 cut(s) 220, 323, 404, 549
Bst6I CTCTTC 1 cut(s) 213
BstC8I GCNNGC 3 cut(s) 810, 906, 921
BstDEI CTNAG 1 cut(s) 956
BstEII GGTNACC 1 cut(s) 604
BstF5I GGATG 1 cut(s) 927
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 5 cut(s) 295, 640, 713, 888, 970
BstMBI GATC 5 cut(s) 292, 637, 710, 885, 967
BstNSI RCATGY 1 cut(s) 908
BstPI GGTNACC 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 295
BstSLI GKGCMC 1 cut(s) 409
BstV1I GCAGC 2 cut(s) 289, 361
BstX2I RGATCY 2 cut(s) 292, 710
BstXI CCANNNNNNTGG 1 cut(s) 707
BstYI RGATCY 2 cut(s) 292, 710
BsuI GTATCC 1 cut(s) 675
BsuRI GGCC 2 cut(s) 105, 313
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 1 cut(s) 927
BtsIMutI CAGTG 1 cut(s) 554
Cac8I GCNNGC 3 cut(s) 810, 906, 921
CaiI CAGNNNCTG 1 cut(s) 590
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 2 cut(s) 299, 312
CseI GACGC 3 cut(s) 34, 238, 731
Csp6I GTAC 5 cut(s) 124, 324, 400, 422, 945
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviAII CATG 9 cut(s) 45, 187, 282, 488, 611, 623, 802, 905, 965
CviQI GTAC 5 cut(s) 124, 324, 400, 422, 945
DdeI CTNAG 1 cut(s) 956
DpnI GATC 5 cut(s) 294, 639, 712, 887, 969
DpnII GATC 5 cut(s) 292, 637, 710, 885, 967
DraI TTTAAA 1 cut(s) 694
DriI GACNNNNNGTC 1 cut(s) 741
Eam1104I CTCTTC 1 cut(s) 213
Eam1105I GACNNNNNGTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 299
Eco53kI GAGCTC 1 cut(s) 184
Eco91I GGTNACC 1 cut(s) 604
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 604
EcoT22I ATGCAT 2 cut(s) 910, 990
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 9 cut(s) 48, 190, 285, 491, 614, 626, 805, 908, 968
FaqI GGGAC 2 cut(s) 208, 285
FatI CATG 9 cut(s) 44, 186, 281, 487, 610, 622, 801, 904, 964
FbaI TGATCA 1 cut(s) 885
Fnu4HI GCNGC 2 cut(s) 278, 375
FokI GGATG 1 cut(s) 914
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 278, 375
FspBI CTAG 7 cut(s) 29, 59, 338, 476, 746, 920, 950
GlaI GCGC 1 cut(s) 813
GluI GCNGC 2 cut(s) 278, 375
GsaI CCCAGC 2 cut(s) 120, 381
HaeIII GGCC 2 cut(s) 105, 313
HapII CCGG 1 cut(s) 296
HgaI GACGC 3 cut(s) 34, 238, 731
HhaI GCGC 1 cut(s) 814
Hin1II CATG 9 cut(s) 48, 190, 285, 491, 614, 626, 805, 908, 968
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 5 cut(s) 145, 257, 587, 669, 760
HpaII CCGG 1 cut(s) 296
HphI GGTGA 3 cut(s) 229, 395, 598
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 1 cut(s) 727
Hpy188III TCNNGA 5 cut(s) 130, 392, 617, 757, 794
Hpy8I GTNNAC 1 cut(s) 36
HpyAV CCTTC 4 cut(s) 11, 116, 169, 505
HpyCH4III ACNGT 4 cut(s) 220, 323, 404, 549
HpyCH4V TGCA 8 cut(s) 285, 457, 566, 768, 904, 908, 988, 998
HpyF3I CTNAG 1 cut(s) 956
Hsp92II CATG 9 cut(s) 48, 190, 285, 491, 614, 626, 805, 908, 968
HspAI GCGC 1 cut(s) 812
KflI GGGWCCC 1 cut(s) 299
KpnI GGTACC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 885
Kzo9I GATC 5 cut(s) 292, 637, 710, 885, 967
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 9 cut(s) 102, 155, 239, 303, 309, 336, 356, 363, 901
Lsp1109I GCAGC 2 cut(s) 289, 361
LweI GCATC 1 cut(s) 90
MaeI CTAG 7 cut(s) 29, 59, 338, 476, 746, 920, 950
MaeIII GTNAC 4 cut(s) 22, 490, 604, 715
MalI GATC 5 cut(s) 294, 639, 712, 887, 969
MboI GATC 5 cut(s) 292, 637, 710, 885, 967
MboII GAAGA 3 cut(s) 145, 148, 200
MflI RGATCY 2 cut(s) 292, 710
MhlI GDGCHC 2 cut(s) 186, 409
MluCI AATT 3 cut(s) 355, 661, 701
MlyI GAGTC 2 cut(s) 266, 754
MmeI TCCRAC 2 cut(s) 65, 659
MnlI CCTC 8 cut(s) 5, 95, 151, 172, 357, 379, 388, 547
Mph1103I ATGCAT 2 cut(s) 910, 990
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 3 cut(s) 359, 693, 1002
MslI CAYNNNNRTG 2 cut(s) 61, 242
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 297
NciI CCSGG 1 cut(s) 297
NdeII GATC 5 cut(s) 292, 637, 710, 885, 967
NheI GCTAGC 1 cut(s) 919
NlaIII CATG 9 cut(s) 48, 190, 285, 491, 614, 626, 805, 908, 968
NlaIV GGNNCC 3 cut(s) 300, 301, 423
NmuCI GTSAC 4 cut(s) 22, 490, 604, 715
NsiI ATGCAT 2 cut(s) 910, 990
NspI RCATGY 1 cut(s) 908
PaeI GCATGC 1 cut(s) 908
PceI AGGCCT 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 3 cut(s) 145, 587, 669
PflFI GACNNNGTC 1 cut(s) 220
PkrI GCNGC 2 cut(s) 279, 376
PleI GAGTC 2 cut(s) 265, 754
PpsI GAGTC 2 cut(s) 265, 754
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 299
PspEI GGTNACC 1 cut(s) 604
PspFI CCCAGC 2 cut(s) 116, 377
PspN4I GGNNCC 3 cut(s) 300, 301, 423
PspPI GGNCC 2 cut(s) 299, 312
PspPPI RGGWCCY 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 2 cut(s) 292, 710
PsyI GACNNNGTC 1 cut(s) 220
RsaI GTAC 5 cut(s) 125, 325, 401, 423, 946
RsaNI GTAC 5 cut(s) 124, 324, 400, 422, 945
RseI CAYNNNNRTG 2 cut(s) 61, 242
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 3 cut(s) 359, 693, 1002
SatI GCNGC 2 cut(s) 278, 375
Sau3AI GATC 5 cut(s) 292, 637, 710, 885, 967
Sau96I GGNCC 2 cut(s) 299, 312
ScaI AGTACT 2 cut(s) 125, 946
SchI GAGTC 2 cut(s) 266, 754
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 2 cut(s) 186, 409
SetI ASST 8 cut(s) 22, 186, 339, 423, 445, 497, 606, 955
SfaNI GCATC 1 cut(s) 90
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 61, 242
SmlI CTYRAG 1 cut(s) 390
SmoI CTYRAG 1 cut(s) 390
SpeI ACTAGT 1 cut(s) 58
SphI GCATGC 1 cut(s) 908
Sse9I AATT 3 cut(s) 355, 661, 701
SseBI AGGCCT 1 cut(s) 105
SsiI CCGC 1 cut(s) 149
SspMI CTAG 7 cut(s) 29, 59, 338, 476, 746, 920, 950
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 4 cut(s) 220, 323, 404, 549
TaqI TCGA 2 cut(s) 667, 758
TasI AATT 3 cut(s) 355, 661, 701
TatI WGTACW 2 cut(s) 123, 944
TfiI GAWTC 3 cut(s) 145, 587, 669
Tru1I TTAA 3 cut(s) 359, 693, 1002
Tru9I TTAA 3 cut(s) 359, 693, 1002
TscAI CASTG 1 cut(s) 554
TseFI GTSAC 4 cut(s) 22, 490, 604, 715
TseI GCWGC 2 cut(s) 277, 374
Tsp45I GTSAC 4 cut(s) 22, 490, 604, 715
TspDTI ATGAA 4 cut(s) 58, 742, 916, 969
TspRI CASTG 1 cut(s) 554
Tth111I GACNNNGTC 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 908
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 7 cut(s) 29, 59, 338, 476, 746, 920, 950
ZrmI AGTACT 2 cut(s) 125, 946
Zsp2I ATGCAT 2 cut(s) 910, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.