Rh1CG162000

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0015815
Physical Location & Seq
Forward (+)
35919911 .. 35928315
8405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG162000.1

Sequence Viewer

Length: 1347 bp
ATGGCTGGTGGAGGCGAAGGTGTGACGCTAGAGTTCACTCCAACATGGGTGGTCGCACTAGTATGCTTCATCATAGTCAGCATCTCTCTTTTGGCAGAGAGAGGCCTTCACTCTGCTGGGAAGTACTTCAAGAAGAAGAACCAGAATCCGCTGTATGAGGCATTGCTGAAAGTGAAGGAGGAGCTCATGTTGTTGGGCTTTATCTCTCTTCTACTGACTGTGTCCCAGAAAACCATCACCAAAATGTGCGTCAAAGAGTCATTATATAAGTCGTGGCAGCCATGCAGACCAGATCCGGGTCCCCATAATAGGCCCAAAAACAGTACCCATTTTGAGCTAGAAAGTATTTCTGGGAATTTAAGGAGGCTTCTGGCTGCTGGGGGTGAGGCTCAAGAGGGGTACTGTGCCCAAAAGGGTAAGGTACCATTGTTATCAGTTCAAGCTTTCCATCATCTGCATATCTTTATATTTGTCCTAGCCATCGCTCATGTGACCTTCTCTGCTATGACTATTTGTTTCGGTGGGCTAAAGATACGCCAATGGAAACAGTGGGAGGAGTTGGTTGCAAAAGAGATTGATGATACAGAATCTGCTAAACCAAAGGTCACCCATGTTCAAGACCATGATTTTGTCAAAGATCATTATTTGGGTATTGGTAAAAATTATCGAATCAAGGGTTGGATACATTCTTTTAAAAACCAATTTTATGGATCTGTGACCAAATCAGATTATTTGACTTTGCGTCTAGGCTTCATCTCGACTCATTGCAAGGAAAATCCCAAGTTTGATTTTCACGATTACATGGTGCGTGCGCTTGAAAATGATTTCAAAAAAGTTGTCGGTATCAGTTGGTATCTATGGTTATTCGTAGTCATCTTTCTGCTGATCAATGTTGATGGTTTGCATGCATACTTCTGGCTAGCGTTCATCCCTTTCTTTCTTCTACTTGCTGTAGGAGCTAAGCTACAACATATAATCACCCAGTTGGCTCAAGAAGTTGCTGAGAAACATATAGCAGTAGAAGGGGATCTAATAGTTCAACCATCAGACGAACATTTTTGGTTTAGTCGACCCAGACTTGTTATCTCCTTGCTCCATTTCATTCTTTTCCAAAATGCTTTCGAGATGGCATTTTTCATTTGGATATGGCTTACATATGGTTTTGACTCTTGCATAATGGGGAGAATCCGATACATTGTCCCCAGGATTGTTATAGGGTTGTTCATTCAGATCCTCTGTAGTTACAGCACTTTACCACTGTATGCCATTGTCACACAGATGGGATCCAATTTCAAGCAAGCAATATTCAATAACTCAAACAGCGGAGTGAAGGAACGCAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

448

Amino Acids

51.32

Weight (kDa)

9.05

Isoelectric Point (pI)

23.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 439 8.9e-181 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015815)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08130 FvH4_7g08130
malus_domestica MD02G1230200.v1.1 MD07G1082000.v1.1
prunus_persica Prupe.2G107600_v2.0.a1 Prupe.2G107600_v2.0.a1
pyrus_communis pycom07g06530
rosa_chinensis RchiOBHm_Chr1g0341101
rosa_laevigata RLG00000029064
rosa_multiflora Rmu_sc0001211.1_g000067
rosa_rugosa Rorug01G0159900.1
rosa_samantha Rh1AG174200 Rh1BG142500 Rh1CG162000
rosa_wichuraiana Rw1G014530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 421
AccB1I GGYRCC 1 cut(s) 421
AccI GTMKAC 1 cut(s) 1069
AciI CCGC 2 cut(s) 149, 1323
AclWI GGATC 6 cut(s) 287, 718, 1035, 1225, 1278, 1291
AcsI RAATTY 1 cut(s) 355
AfaI GTAC 4 cut(s) 125, 325, 401, 423
AfiI CCNNNNNNNGG 2 cut(s) 296, 309
AgsI TTSAA 8 cut(s) 130, 440, 617, 818, 829, 1040, 1294, 1309
AhdI GACNNNNNGTC 1 cut(s) 741
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 1 cut(s) 1202
AjuI GAANNNNNNNTTGG 2 cut(s) 661, 693
AluBI AGCT 5 cut(s) 184, 337, 443, 959, 964
AluI AGCT 5 cut(s) 184, 337, 443, 959, 964
Alw21I GWGCWC 1 cut(s) 186
AlwI GGATC 6 cut(s) 287, 718, 1035, 1225, 1278, 1291
AlwNI CAGNNNCTG 1 cut(s) 590
AoxI GGCC 2 cut(s) 103, 311
ApeKI GCWGC 2 cut(s) 277, 374
ApoI RAATTY 1 cut(s) 355
ArsI GACNNNNNNTTYG 6 cut(s) 234, 266, 611, 643, 822, 854
Asp700I GAANNNNTTC 1 cut(s) 125
Asp718I GGTACC 1 cut(s) 421
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 2 cut(s) 299, 312
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 4 cut(s) 229, 395, 598, 970
AsuNHI GCTAGC 1 cut(s) 919
AvaII GGWCC 1 cut(s) 299
BaeGI GKGCMC 1 cut(s) 409
BaeI ACNNNNGTAYC 2 cut(s) 413, 446
BamHI GGATCC 1 cut(s) 1283
BanI GGYRCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 289, 361
BccI CCATC 7 cut(s) 242, 456, 488, 890, 1051, 1120, 1273
BciT130I CCWGG 1 cut(s) 1204
BciVI GTATCC 1 cut(s) 675
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 1 cut(s) 297
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 6 cut(s) 29, 59, 338, 476, 746, 920
BfmI CTRYAG 2 cut(s) 951, 1237
BfuI GTATCC 1 cut(s) 675
BisI GCNGC 2 cut(s) 278, 375
BlpI GCTNAGC 1 cut(s) 960
BlsI GCNGC 2 cut(s) 279, 376
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 297, 1204
Bme18I GGWCC 1 cut(s) 299
BmeRI GACNNNNNGTC 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 299, 312
BmiI GGNNCC 4 cut(s) 300, 301, 423, 1285
BmrFI CCNGG 2 cut(s) 297, 1204
BmrI ACTGGG 1 cut(s) 976
BmsI GCATC 1 cut(s) 90
BmtI GCTAGC 1 cut(s) 923
BmuI ACTGGG 1 cut(s) 976
Bpu1102I GCTNAGC 1 cut(s) 960
BpuEI CTTGAG 2 cut(s) 375, 975
BpuMI CCSGG 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 1202
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 309
Bse1I ACTGG 1 cut(s) 982
Bse3DI GCAATG 2 cut(s) 161, 763
BseBI CCWGG 1 cut(s) 1204
BseDI CCNNGG 1 cut(s) 1202
BseGI GGATG 1 cut(s) 927
BseLI CCNNNNNNNGG 2 cut(s) 296, 309
BseMI GCAATG 2 cut(s) 161, 763
BseMII CTCAG 1 cut(s) 993
BseNI ACTGG 1 cut(s) 982
BseRI GAGGAG 2 cut(s) 194, 569
BseSI GKGCMC 1 cut(s) 409
BseXI GCAGC 2 cut(s) 289, 361
BseYI CCCAGC 2 cut(s) 116, 377
BshFI GGCC 2 cut(s) 105, 313
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 3 cut(s) 208, 285, 1184
BslI CCNNNNNNNGG 2 cut(s) 296, 309
BsmFI GGGAC 3 cut(s) 208, 285, 1184
BsnI GGCC 2 cut(s) 105, 313
Bsp1286I GDGCHC 2 cut(s) 186, 409
Bsp143I GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
Bsp1720I GCTNAGC 1 cut(s) 960
BspACI CCGC 2 cut(s) 149, 1323
BspANI GGCC 2 cut(s) 105, 313
BspCNI CTCAG 1 cut(s) 994
BspLI GGNNCC 4 cut(s) 300, 301, 423, 1285
BspOI GCTAGC 1 cut(s) 923
BspPI GGATC 6 cut(s) 287, 718, 1035, 1225, 1278, 1291
BspT107I GGYRCC 1 cut(s) 421
BsrDI GCAATG 2 cut(s) 161, 763
BsrI ACTGG 1 cut(s) 982
BssECI CCNNGG 1 cut(s) 1202
BssMI GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
Bst2UI CCWGG 1 cut(s) 1204
Bst4CI ACNGT 5 cut(s) 220, 323, 404, 549, 1260
Bst6I CTCTTC 1 cut(s) 213
BstC8I GCNNGC 4 cut(s) 810, 906, 921, 1299
BstDEI CTNAG 2 cut(s) 960, 1002
BstEII GGTNACC 1 cut(s) 604
BstF5I GGATG 1 cut(s) 927
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 7 cut(s) 295, 640, 713, 888, 1030, 1233, 1286
BstMBI GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
BstMWI GCNNNNNNNGC 1 cut(s) 956
BstNI CCWGG 1 cut(s) 1204
BstNSI RCATGY 1 cut(s) 908
BstPI GGTNACC 1 cut(s) 604
BstSCI CCNGG 2 cut(s) 295, 1202
BstSFI CTRYAG 2 cut(s) 951, 1237
BstSLI GKGCMC 1 cut(s) 409
BstV1I GCAGC 2 cut(s) 289, 361
BstX2I RGATCY 5 cut(s) 292, 710, 1027, 1230, 1283
BstXI CCANNNNNNTGG 1 cut(s) 707
BstYI RGATCY 5 cut(s) 292, 710, 1027, 1230, 1283
BsuI GTATCC 1 cut(s) 675
BsuRI GGCC 2 cut(s) 105, 313
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 1 cut(s) 927
BtsIMutI CAGTG 2 cut(s) 554, 1256
Cac8I GCNNGC 4 cut(s) 810, 906, 921, 1299
CaiI CAGNNNCTG 1 cut(s) 590
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 2 cut(s) 299, 312
CseI GACGC 3 cut(s) 34, 238, 731
Csp6I GTAC 4 cut(s) 124, 324, 400, 422
CspCI CAANNNNNGTGG 2 cut(s) 30, 65
CviAII CATG 8 cut(s) 45, 187, 282, 488, 611, 623, 802, 905
CviQI GTAC 4 cut(s) 124, 324, 400, 422
DdeI CTNAG 2 cut(s) 960, 1002
DpnI GATC 7 cut(s) 294, 639, 712, 887, 1029, 1232, 1285
DpnII GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
DraI TTTAAA 1 cut(s) 694
DriI GACNNNNNGTC 1 cut(s) 741
Eam1104I CTCTTC 1 cut(s) 213
Eam1105I GACNNNNNGTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 299
Eco53kI GAGCTC 1 cut(s) 184
Eco91I GGTNACC 1 cut(s) 604
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 604
EcoRII CCWGG 1 cut(s) 1202
EcoT22I ATGCAT 1 cut(s) 910
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 8 cut(s) 48, 190, 285, 491, 614, 626, 805, 908
FaqI GGGAC 3 cut(s) 208, 285, 1184
FatI CATG 8 cut(s) 44, 186, 281, 487, 610, 622, 801, 904
FauNDI CATATG 1 cut(s) 1156
FbaI TGATCA 1 cut(s) 885
FblI GTMKAC 1 cut(s) 1069
Fnu4HI GCNGC 2 cut(s) 278, 375
FokI GGATG 1 cut(s) 914
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 278, 375
FspBI CTAG 6 cut(s) 29, 59, 338, 476, 746, 920
GlaI GCGC 1 cut(s) 813
GluI GCNGC 2 cut(s) 278, 375
GsaI CCCAGC 2 cut(s) 120, 381
HaeIII GGCC 2 cut(s) 105, 313
HapII CCGG 1 cut(s) 296
HgaI GACGC 3 cut(s) 34, 238, 731
HhaI GCGC 1 cut(s) 814
Hin1II CATG 8 cut(s) 48, 190, 285, 491, 614, 626, 805, 908
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 1070
HindII GTYRAC 1 cut(s) 1070
HindIII AAGCTT 1 cut(s) 441
HinfI GANTC 7 cut(s) 145, 257, 587, 669, 760, 1166, 1185
HpaII CCGG 1 cut(s) 296
HphI GGTGA 4 cut(s) 229, 395, 598, 970
Hpy166II GTNNAC 2 cut(s) 36, 1070
Hpy188I TCNGA 4 cut(s) 727, 1048, 1190, 1230
Hpy188III TCNNGA 7 cut(s) 130, 392, 617, 757, 794, 992, 1123
Hpy8I GTNNAC 2 cut(s) 36, 1070
HpyAV CCTTC 6 cut(s) 11, 116, 169, 505, 1016, 1324
HpyCH4III ACNGT 5 cut(s) 220, 323, 404, 549, 1260
HpyCH4V TGCA 7 cut(s) 285, 457, 566, 768, 904, 908, 1173
HpyF10VI GCNNNNNNNGC 1 cut(s) 956
HpyF3I CTNAG 2 cut(s) 960, 1002
Hsp92II CATG 8 cut(s) 48, 190, 285, 491, 614, 626, 805, 908
HspAI GCGC 1 cut(s) 812
KflI GGGWCCC 1 cut(s) 299
KpnI GGTACC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 885
Kzo9I GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
LmnI GCTCC 3 cut(s) 181, 956, 1098
Lsp1109I GCAGC 2 cut(s) 289, 361
LweI GCATC 1 cut(s) 90
MaeI CTAG 6 cut(s) 29, 59, 338, 476, 746, 920
MaeIII GTNAC 6 cut(s) 22, 490, 604, 715, 1241, 1270
MalI GATC 7 cut(s) 294, 639, 712, 887, 1029, 1232, 1285
MboI GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
MboII GAAGA 4 cut(s) 145, 148, 200, 932
MflI RGATCY 5 cut(s) 292, 710, 1027, 1230, 1283
MhlI GDGCHC 2 cut(s) 186, 409
MluCI AATT 4 cut(s) 355, 661, 701, 1288
MlyI GAGTC 3 cut(s) 266, 754, 1160
MmeI TCCRAC 2 cut(s) 65, 659
MnlI CCTC 9 cut(s) 5, 95, 151, 172, 357, 379, 388, 547, 1244
Mph1103I ATGCAT 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 3 cut(s) 359, 693, 1345
MslI CAYNNNNRTG 3 cut(s) 61, 242, 1277
MspA1I CMGCKG 2 cut(s) 151, 1323
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 297, 1204
MvaI CCWGG 1 cut(s) 1204
MwoI GCNNNNNNNGC 1 cut(s) 956
NciI CCSGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 1156
NdeII GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
NheI GCTAGC 1 cut(s) 919
NlaIII CATG 8 cut(s) 48, 190, 285, 491, 614, 626, 805, 908
NlaIV GGNNCC 4 cut(s) 300, 301, 423, 1285
NmuCI GTSAC 5 cut(s) 22, 490, 604, 715, 1270
NsiI ATGCAT 1 cut(s) 910
NspI RCATGY 1 cut(s) 908
PaeI GCATGC 1 cut(s) 908
PceI AGGCCT 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 4 cut(s) 145, 587, 669, 1185
PflFI GACNNNGTC 1 cut(s) 220
PkrI GCNGC 2 cut(s) 279, 376
PleI GAGTC 3 cut(s) 265, 754, 1160
PpsI GAGTC 3 cut(s) 265, 754, 1160
PpuMI RGGWCCY 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 1202
PspEI GGTNACC 1 cut(s) 604
PspFI CCCAGC 2 cut(s) 116, 377
PspGI CCWGG 1 cut(s) 1202
PspN4I GGNNCC 4 cut(s) 300, 301, 423, 1285
PspPI GGNCC 2 cut(s) 299, 312
PspPPI RGGWCCY 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 5 cut(s) 292, 710, 1027, 1230, 1283
PsyI GACNNNGTC 1 cut(s) 220
RsaI GTAC 4 cut(s) 125, 325, 401, 423
RsaNI GTAC 4 cut(s) 124, 324, 400, 422
RseI CAYNNNNRTG 3 cut(s) 61, 242, 1277
SacI GAGCTC 1 cut(s) 186
SalI GTCGAC 1 cut(s) 1068
SaqAI TTAA 3 cut(s) 359, 693, 1345
SatI GCNGC 2 cut(s) 278, 375
Sau3AI GATC 7 cut(s) 292, 637, 710, 885, 1027, 1230, 1283
Sau96I GGNCC 2 cut(s) 299, 312
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 3 cut(s) 266, 754, 1160
ScrFI CCNGG 2 cut(s) 297, 1204
SduI GDGCHC 2 cut(s) 186, 409
SetI ASST 9 cut(s) 22, 186, 339, 423, 445, 497, 606, 961, 966
SfaNI GCATC 1 cut(s) 90
SfcI CTRYAG 2 cut(s) 951, 1237
SinI GGWCC 1 cut(s) 299
SmiMI CAYNNNNRTG 3 cut(s) 61, 242, 1277
SmlI CTYRAG 2 cut(s) 390, 990
SmoI CTYRAG 2 cut(s) 390, 990
SpeI ACTAGT 1 cut(s) 58
SphI GCATGC 1 cut(s) 908
Sse9I AATT 4 cut(s) 355, 661, 701, 1288
SseBI AGGCCT 1 cut(s) 105
SsiI CCGC 2 cut(s) 149, 1323
SspI AATATT 1 cut(s) 1305
SspMI CTAG 6 cut(s) 29, 59, 338, 476, 746, 920
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 2 cut(s) 295, 1202
TaaI ACNGT 5 cut(s) 220, 323, 404, 549, 1260
TaqI TCGA 4 cut(s) 667, 758, 1069, 1122
TasI AATT 4 cut(s) 355, 661, 701, 1288
TatI WGTACW 1 cut(s) 123
TfiI GAWTC 4 cut(s) 145, 587, 669, 1185
Tru1I TTAA 3 cut(s) 359, 693, 1345
Tru9I TTAA 3 cut(s) 359, 693, 1345
TscAI CASTG 2 cut(s) 554, 1263
TseFI GTSAC 5 cut(s) 22, 490, 604, 715, 1270
TseI GCWGC 2 cut(s) 277, 374
Tsp45I GTSAC 5 cut(s) 22, 490, 604, 715, 1270
TspDTI ATGAA 6 cut(s) 58, 742, 916, 1090, 1126, 1213
TspRI CASTG 2 cut(s) 554, 1263
Tth111I GACNNNGTC 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 908
XmiI GTMKAC 1 cut(s) 1069
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 6 cut(s) 29, 59, 338, 476, 746, 920
ZrmI AGTACT 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.