FvH4_7g22680

Belongs to the adaptor complexes large subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009227
Physical Location & Seq
Reverse (-)
17848484 .. 17854781
6298 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22680.t4

Sequence Viewer

Length: 3060 bp
ATGTTCAGTCAATTCGGCGCCACCGCCGATAATCTAAGCAAGGCGTCGGCAGTCGTGTTCCGGATCGGCACCGACGCCCACCTCTACGACGACCCCGAGGATGTCAGCATCGCTCCTCTCCTCGACAGCAAGTTCGACTCCGAGAAGTGCGAAGCTCTCAAACGCCTCCTCGCTCTCATCGCCCAAGGCTTCGACGTCTCCAATTTCTTCCCTCAGGTTGTTAAGAACGTAGCGACTCAGTCGCTGGAAGTGAAGAAGCTGGTGTACTTGTACCTGTTGCATTATGCTCACAAGCGTCCGAATGAGGCGTTGCTGTCGATTAATTGCTTCCAGAAGGATCTAGGGGATCCGAACCCGTTGGTGAGGGCATGGGCGCTGCGAGCCATGGCCGGGATCCGGCTGCATGTTATTGCGCCTTTGGTTATGGTTGCTGTGGGGAAATGTGCTAGAGATCCGTCTGTGTATGTTCGGAAATGCGCTGCTAATGCACTTCCTAAGCTGCATGATTTGCGGCTCGATGAGTATACTGCCAGTATTGAAGAGGTTATTGGAATACTGTTGAATGACCATTCCCCTTGTGTTGTTGGAGCTGCTGCAGCTGCCTTCTCTTCAATTTGTCCAAATAATATGTCTTTGATTGGAAGAAACTATCACAGGCTATGCGAGATTCTTCCTGATGTCGAAGAATGGGGTCAAATAGTTTTAATTGGGATCCTTCTGCGCTATGTAATTGCAAGGCATGGGTTTGTGCAGGAATCCATTATGGCTTCTTTGCATCATACAGAGAATTGTAAATCTCAGAAGGATTTCTGCGACACCAATTCTGTATTAGAAGACAATGGTGCCATGAGTGGTCTTCATGAGTCTGAATTGGCAAATGTTGTCTTTCGATGTTATATTGAAGGGCCAGATGAATATTTATCACGAGTGGGTTTCATGAATAAAGATTCCTCTGAATTCAATCCACGTGTTACATCTGGAAACAATAATGAGGATATGACGTTCCTGCTGCGATGTACCTCACCATTGTTATGGAGCAATAATAGTGCAGTTGTACTAGCAGCTGCTGGTGTTCACTGGATTATGTCACCGATAGAGGAAGTAAAAAGAATTGTTAAACCACTCTTGTTCGTCCAAAGATCATCTACTGCCTCAAAATATGTGGTTTTGTGCAACATTCAAGTCTTTGCTAAAGCAATTCCTTCACTCTTCTCTCCATACTTTGAAGATTTCTTCATATGCTCATCAGATTCGTATCAAATTAAAGCCTTGAAACTTGATATACTGGCTCACATCGTTACAGATTCATCAATTCCATTTGTTCTCAAAGAGTTTCAGGATTATATTAGAGATCCAGACAGGAGATTTGCTGCTGACACTGTTGCTGGAATTGGTATCTGTGCACAACGACTTCCAAATATGGCAAACACATGCTTGGAATTTCTATTGGCTTTGACCAGACAGCAAGTTATGACTGGGGAATTTGGGTCTGTGGAGGGAGAAGCAAATATTTTGATTCAAGCAATTATATCAATTAAATCAATCGTTCAGCAAGATCCACCCAGTTATGAAAAGGTAATTATTCAGTTGGTTCGCAGTCTGAATTCAGTAAAGGTGCCCGCAGCTCGTGCAATGATTGTTTGGATGGTGGGGGAGTATAACTCTTTAGGCGATATGATTCCAAGGATGGTAACTACAGTACTCAAGTATCTTGCAAGGTGCTTTACTTCAGAAGAGTTGGAGACAAAGCTTCAAATTTGTAATACTACTGTTAAGGTTTTATTGCATGCTGAAGGGAATGATCAGTCAACTATCCAAAAAGTTTTGAGCTATGTGCTGGAACTGGCCAAGTATGACTTATGCTACGATGTTCGTGACCGTGCTTATTTCCTAAAGAATCTTTTGTCATCTTATCTGGATTCTCAAGGTCTGAAGGAGGAAAACAATAATCTGTCACAAGATAAAGATATTCCGTGTGTCCTTGCAAAATACTTGTTTGGAGGACAAACAAAATCAAATTCATCTGAGCCCATTGATCACCGGTTTTATCTTCCTGGCTCTTTATCACAAATAGTACTTCATGCTGCTCCAGGGTATGAACCTCTTCCGAAGCCCTGTACTATGCTCTCTGATGGCCTCAAAAATGAATTTGGGGAGGGAGTCACTAGTGAAACATCTGTTACAGATGATCAGAATTCAGTCTCTGAGTCTTTGGATGAGGAGAATTCCTCTACTTATAGTTCACACCATTCTGATGCCAGTGGCAGTGGTGACAGTGAAGAGGATGCTTCAGCAAGTGAAGATGACAATTCTAATCCGTTGATTCAATTAGCAGATGCTGGCAATGCGCATGAAGTGAAAAATGGAGCTTCTCAATCTGCTTCTGATTTTGGGGAATTGTTGTCAAAGAGAGCTCTGGAATCATGGTTGGACGAACAGCCTGGTTTTTCGAGCTCAAATAATCCAGAACAAAGTCAAGTCAATAGATCTTCGGCTAGAATCTCCATTGGGGATGTCGGAGGACAAGTTAAACCTAAAAGCTATTCACTTTTGGACACTGTGAATGGAAATGGCTTAAAGGTTGACTACTCATTTTCATCTGAGATTTCAGACATCTCTCCTCTTTTTATATGTATAGAAGCTTCCTTCAAGAATTGTTCAAACGAGATCATGTCTGATATAAATTTGGTTGACGAGGAATCTGACAAAGGCACAAACTCTGGAGACCAGGCTTCGGTTACACATGAAAGCTCCATGATATCTCAAAATAATGCGTCAAATCTAGCTTCTGTAGAAGAGATCACTTCTCTGGAATCTGGTCAGACAATGACAAGAGTCATCCAAGTTCGCTTCCATCACCACCTCTTGCCTCTAAAACTCACCTTATATTGCAATGGCAAGAGGCATCCTGTTAAGTTGAGGCCTGACATCGGATACTTTGTGAGAGCTCTTCCTTTGGATGTTGATGCCTTCACAATTAAGGAGTCACATCTGCGAGGAATGTTTGAATGCACAAGAAGTTTGAATGTCATATTTTGTGTAACAGGTGCAATTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
AP3B1_C Adaptin_N

Protein Analysis

1020

Amino Acids

112.41

Weight (kDa)

5.15

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 40 - 633 1.3e-97 Adaptin N terminal region
Cnd1 PF12717 120 - 262 4.3e-14 non-SMC mitotic condensation complex subunit 1
AP3B1_C PF14796 818 - 900 1.4e-07 Clathrin-adaptor complex-3 beta-1 subunit C-terminal
AP3B1_C_2 PF24080 977 - 1116 5.1e-06 AP-3 complex subunit beta-1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
Acc16I TGCGCA 1 cut(s) 2349
AccB1I GGYRCC 4 cut(s) 17, 68, 842, 1615
AccI GTMKAC 1 cut(s) 524
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 3 cut(s) 24, 511, 1620
AcoI YGGCCR 2 cut(s) 387, 1845
AcuI CTGAAG 4 cut(s) 1713, 1812, 1952, 2274
AcvI CACGTG 1 cut(s) 968
AcyI GRCGYC 4 cut(s) 18, 44, 75, 195
AfaI GTAC 7 cut(s) 266, 272, 1018, 1056, 1701, 2076, 2119
AfiI CCNNNNNNNGG 4 cut(s) 390, 396, 2734, 2930
AflIII ACRYGT 1 cut(s) 967
AgeI ACCGGT 1 cut(s) 2040
AhlI ACTAGT 1 cut(s) 2165
AjnI CCWGG 4 cut(s) 2053, 2089, 2440, 2727
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
AloI GAACNNNNNNTCC 2 cut(s) 986, 1018
Alw21I GWGCWC 4 cut(s) 1405, 2416, 2456, 2950
Alw26I GTCTC 4 cut(s) 202, 1736, 2206, 2718
Alw44I GTGCAC 1 cut(s) 1401
AlwNI CAGNNNCTG 4 cut(s) 244, 1067, 2204, 2339
Ama87I CYCGRG 1 cut(s) 95
Aor13HI TCCGGA 1 cut(s) 60
AoxI GGCC 5 cut(s) 387, 905, 1845, 2134, 2921
ApaLI GTGCAC 1 cut(s) 1401
ArsI GACNNNNNNTTYG 6 cut(s) 116, 148, 614, 646, 1349, 1381
AseI ATTAAT 1 cut(s) 321
AsiGI ACCGGT 1 cut(s) 2040
Asp700I GAANNNNTTC 2 cut(s) 806, 2103
AspLEI GCGC 6 cut(s) 20, 376, 415, 479, 723, 2350
AspS9I GGNCC 1 cut(s) 905
AsuC2I CCSGG 1 cut(s) 391
AsuHPI GGTGA 7 cut(s) 373, 1014, 1080, 2030, 2282, 2849, 2872
AvaI CYCGRG 1 cut(s) 95
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 2 cut(s) 1405, 1620
BaeI ACNNNNGTAYC 2 cut(s) 1691, 1724
BalI TGGCCA 1 cut(s) 1847
BamHI GGATCC 3 cut(s) 346, 393, 711
BanI GGYRCC 4 cut(s) 17, 68, 842, 1615
BanII GRGCYC 4 cut(s) 2031, 2416, 2456, 2950
BarI GAAGNNNNNNTAC 4 cut(s) 248, 280, 2626, 2658
BauI CACGAG 2 cut(s) 924, 1626
BbrPI CACGTG 1 cut(s) 968
BbsI GAAGAC 2 cut(s) 840, 848
Bbv12I GWGCWC 4 cut(s) 1405, 2416, 2456, 2950
BccI CCATC 4 cut(s) 1639, 1681, 2126, 2862
BciT130I CCWGG 4 cut(s) 2055, 2091, 2442, 2729
BciVI GTATCC 1 cut(s) 2927
BclI TGATCA 3 cut(s) 1801, 2035, 2188
BcnI CCSGG 1 cut(s) 391
BcoDI GTCTC 4 cut(s) 202, 1736, 2206, 2718
BcuI ACTAGT 1 cut(s) 2165
BfaI CTAG 6 cut(s) 341, 447, 1058, 2166, 2496, 2783
BfmI CTRYAG 3 cut(s) 594, 1695, 2790
BfoI RGCGCY 2 cut(s) 21, 377
BfuI GTATCC 1 cut(s) 2927
BglII AGATCT 1 cut(s) 2486
BmcAI AGTACT 2 cut(s) 1701, 2076
Bme1390I CCNGG 5 cut(s) 391, 2055, 2091, 2442, 2729
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 7 cut(s) 19, 70, 348, 395, 713, 844, 1617
BmrFI CCNGG 5 cut(s) 391, 2055, 2091, 2442, 2729
BmrI ACTGGG 2 cut(s) 1485, 1557
BmsI GCATC 7 cut(s) 117, 784, 2245, 2275, 2326, 2914, 2956
BmuI ACTGGG 2 cut(s) 1485, 1557
BoxI GACNNNNGTC 1 cut(s) 2834
BpiI GAAGAC 2 cut(s) 840, 848
BplI GAGNNNNNCTC 4 cut(s) 1646, 1678, 2213, 2245
BpmI CTGGAG 2 cut(s) 2073, 2742
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 1688, 1908
BpuMI CCSGG 1 cut(s) 391
BsaAI YACGTR 1 cut(s) 968
BsaBI GATNNNNATC 1 cut(s) 1254
BsaHI GRCGYC 4 cut(s) 18, 44, 75, 195
BsaI GGTCTC 1 cut(s) 2718
BsaJI CCNNGG 5 cut(s) 96, 184, 384, 1682, 2090
BsaWI WCCGGW 2 cut(s) 60, 2040
BsaXI ACNNNNNCTCC 2 cut(s) 2511, 2541
Bsc4I CCNNNNNNNGG 4 cut(s) 390, 396, 2734, 2930
Bse118I RCCGGY 1 cut(s) 2040
Bse1I ACTGG 7 cut(s) 531, 1082, 1290, 1480, 1563, 1848, 2259
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 3 cut(s) 1638, 2350, 2899
Bse8I GATNNNNATC 1 cut(s) 1254
BseAI TCCGGA 1 cut(s) 60
BseBI CCWGG 4 cut(s) 2055, 2091, 2442, 2729
BseDI CCNNGG 5 cut(s) 96, 184, 384, 1682, 2090
BseGI GGATG 9 cut(s) 106, 1650, 1692, 2221, 2290, 2518, 2838, 2905, 2965
BseJI GATNNNNATC 1 cut(s) 1254
BseLI CCNNNNNNNGG 4 cut(s) 390, 396, 2734, 2930
BseMI GCAATG 3 cut(s) 1638, 2350, 2899
BseMII CTCAG 6 cut(s) 227, 251, 812, 2016, 2196, 2592
BseNI ACTGG 7 cut(s) 531, 1082, 1290, 1480, 1563, 1848, 2259
BseRI GAGGAG 5 cut(s) 105, 110, 158, 2234, 2610
BseSI GKGCMC 2 cut(s) 1405, 1620
BsgI GTGCAG 2 cut(s) 770, 1068
BshFI GGCC 5 cut(s) 389, 907, 1847, 2136, 2923
BshNI GGYRCC 4 cut(s) 17, 68, 842, 1615
BshTI ACCGGT 1 cut(s) 2040
BsiHKAI GWGCWC 4 cut(s) 1405, 2416, 2456, 2950
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 4 cut(s) 61, 390, 397, 2041
BslI CCNNNNNNNGG 4 cut(s) 390, 396, 2734, 2930
BsmAI GTCTC 4 cut(s) 202, 1736, 2206, 2718
BsmBI CGTCTC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 3014
BsnI GGCC 5 cut(s) 389, 907, 1847, 2136, 2923
Bso31I GGTCTC 1 cut(s) 2718
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 6 cut(s) 1405, 1620, 2031, 2416, 2456, 2950
Bsp13I TCCGGA 1 cut(s) 60
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 3 cut(s) 24, 511, 1620
BspANI GGCC 5 cut(s) 389, 907, 1847, 2136, 2923
BspCNI CTCAG 6 cut(s) 226, 250, 811, 2017, 2197, 2593
BspEI TCCGGA 1 cut(s) 60
BspHI TCATGA 2 cut(s) 859, 936
BspLI GGNNCC 7 cut(s) 19, 70, 348, 395, 713, 844, 1617
BspMAI CTGCAG 1 cut(s) 598
BspQI GCTCTTC 1 cut(s) 2955
BspT107I GGYRCC 4 cut(s) 17, 68, 842, 1615
BspTNI GGTCTC 1 cut(s) 2718
BsrDI GCAATG 3 cut(s) 1638, 2350, 2899
BsrFI RCCGGY 1 cut(s) 2040
BsrI ACTGG 7 cut(s) 531, 1082, 1290, 1480, 1563, 1848, 2259
BssAI RCCGGY 1 cut(s) 2040
BssECI CCNNGG 5 cut(s) 96, 184, 384, 1682, 2090
BssNAI GTATAC 1 cut(s) 525
BssNI GRCGYC 4 cut(s) 18, 44, 75, 195
BssSI CACGAG 2 cut(s) 924, 1626
BssT1I CCWWGG 3 cut(s) 184, 384, 1682
Bst1107I GTATAC 1 cut(s) 525
Bst2BI CACGAG 2 cut(s) 924, 1626
Bst2UI CCWGG 4 cut(s) 2055, 2091, 2442, 2729
Bst4CI ACNGT 7 cut(s) 558, 1381, 1699, 1771, 1880, 2276, 2560
Bst6I CTCTTC 8 cut(s) 534, 613, 1214, 1728, 2109, 2274, 2790, 2955
BstACI GRCGYC 4 cut(s) 18, 44, 75, 195
BstAPI GCANNNNNTGC 2 cut(s) 508, 1628
BstBAI YACGTR 1 cut(s) 968
BstC8I GCNNGC 4 cut(s) 381, 1620, 1788, 2341
BstDEI CTNAG 8 cut(s) 35, 213, 237, 495, 798, 2025, 2205, 2601
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 9 cut(s) 106, 1650, 1692, 2221, 2290, 2518, 2838, 2905, 2965
BstH2I RGCGCY 2 cut(s) 21, 377
BstHHI GCGC 6 cut(s) 20, 376, 415, 479, 723, 2350
BstMAI GTCTC 4 cut(s) 202, 1736, 2206, 2718
BstMWI GCNNNNNNNGC 8 cut(s) 179, 380, 485, 508, 596, 599, 1628, 2345
BstNI CCWGG 4 cut(s) 2055, 2091, 2442, 2729
BstNSI RCATGY 3 cut(s) 407, 1434, 1790
BstPAI GACNNNNGTC 1 cut(s) 2834
BstSCI CCNGG 5 cut(s) 389, 2053, 2089, 2440, 2727
BstSFI CTRYAG 3 cut(s) 594, 1695, 2790
BstSLI GKGCMC 2 cut(s) 1405, 1620
BstV2I GAAGAC 2 cut(s) 840, 848
BstX2I RGATCY 8 cut(s) 337, 346, 393, 451, 711, 1351, 1555, 2486
BstXI CCANNNNNNTGG 1 cut(s) 1032
BstYI RGATCY 8 cut(s) 337, 346, 393, 451, 711, 1351, 1555, 2486
BstZ17I GTATAC 1 cut(s) 525
Bsu36I CCTNAGG 1 cut(s) 213
BsuI GTATCC 1 cut(s) 2927
BsuRI GGCC 5 cut(s) 389, 907, 1847, 2136, 2923
BtgI CCRYGG 1 cut(s) 384
BtgZI GCGATG 3 cut(s) 94, 163, 1027
BtsCI GGATG 9 cut(s) 106, 1650, 1692, 2221, 2290, 2518, 2838, 2905, 2965
BtsI GCAGTG 1 cut(s) 2272
BtsIMutI CAGTG 6 cut(s) 1075, 1377, 2266, 2272, 2281, 2556
Cac8I GCNNGC 4 cut(s) 381, 1620, 1788, 2341
CaiI CAGNNNCTG 4 cut(s) 244, 1067, 2204, 2339
CciI TCATGA 2 cut(s) 859, 936
CfoI GCGC 6 cut(s) 20, 376, 415, 479, 723, 2350
Cfr10I RCCGGY 1 cut(s) 2040
Cfr13I GGNCC 1 cut(s) 905
CseI GACGC 4 cut(s) 33, 83, 284, 2763
Csp6I GTAC 7 cut(s) 265, 271, 1017, 1055, 1700, 2075, 2118
CspAI ACCGGT 1 cut(s) 2040
CspCI CAANNNNNGTGG 2 cut(s) 1143, 1178
CviQI GTAC 7 cut(s) 265, 271, 1017, 1055, 1700, 2075, 2118
DdeI CTNAG 8 cut(s) 35, 213, 237, 495, 798, 2025, 2205, 2601
DinI GGCGCC 1 cut(s) 19
EaeI YGGCCR 2 cut(s) 387, 1845
Eam1104I CTCTTC 8 cut(s) 534, 613, 1214, 1728, 2109, 2274, 2790, 2955
EarI CTCTTC 8 cut(s) 534, 613, 1214, 1728, 2109, 2274, 2790, 2955
Ecl136II GAGCTC 3 cut(s) 2414, 2454, 2948
Eco130I CCWWGG 3 cut(s) 184, 384, 1682
Eco147I AGGCCT 1 cut(s) 2923
Eco24I GRGCYC 4 cut(s) 2031, 2416, 2456, 2950
Eco31I GGTCTC 1 cut(s) 2718
Eco32I GATATC 1 cut(s) 2760
Eco53kI GAGCTC 3 cut(s) 2414, 2454, 2948
Eco57I CTGAAG 4 cut(s) 1713, 1812, 1952, 2274
Eco72I CACGTG 1 cut(s) 968
Eco81I CCTNAGG 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 3 cut(s) 2414, 2454, 2948
EcoRI GAATTC 4 cut(s) 956, 1603, 2194, 2224
EcoRII CCWGG 4 cut(s) 2053, 2089, 2440, 2727
EcoRV GATATC 1 cut(s) 2760
EcoT14I CCWWGG 3 cut(s) 184, 384, 1682
EcoT38I GRGCYC 4 cut(s) 2031, 2416, 2456, 2950
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
ErhI CCWWGG 3 cut(s) 184, 384, 1682
Esp3I CGTCTC 1 cut(s) 202
FalI AAGNNNNNCTT 2 cut(s) 1841, 1873
FauI CCCGC 1 cut(s) 1627
FauNDI CATATG 1 cut(s) 1238
FbaI TGATCA 3 cut(s) 1801, 2035, 2188
FblI GTMKAC 1 cut(s) 524
FokI GGATG 9 cut(s) 113, 1657, 1699, 2228, 2297, 2525, 2825, 2892, 2972
FriOI GRGCYC 4 cut(s) 2031, 2416, 2456, 2950
FspAI RTGCGCAY 1 cut(s) 2349
FspBI CTAG 6 cut(s) 341, 447, 1058, 2166, 2496, 2783
FspI TGCGCA 1 cut(s) 2349
GlaI GCGC 6 cut(s) 19, 375, 414, 478, 722, 2349
GsuI CTGGAG 2 cut(s) 2073, 2742
HaeII RGCGCY 2 cut(s) 21, 377
HaeIII GGCC 5 cut(s) 389, 907, 1847, 2136, 2923
HapII CCGG 4 cut(s) 61, 390, 397, 2041
HgaI GACGC 4 cut(s) 33, 83, 284, 2763
HhaI GCGC 6 cut(s) 20, 376, 415, 479, 723, 2350
Hin1I GRCGYC 4 cut(s) 18, 44, 75, 195
Hin6I GCGC 6 cut(s) 18, 374, 413, 477, 721, 2348
HinP1I GCGC 6 cut(s) 18, 374, 413, 477, 721, 2348
HincII GTYRAC 3 cut(s) 1809, 2584, 2692
HindII GTYRAC 3 cut(s) 1809, 2584, 2692
HindIII AAGCTT 2 cut(s) 1748, 2640
HpaII CCGG 4 cut(s) 61, 390, 397, 2041
HphI GGTGA 7 cut(s) 373, 1014, 1080, 2030, 2282, 2849, 2872
Hpy166II GTNNAC 8 cut(s) 265, 525, 1075, 1403, 1809, 2243, 2584, 2692
Hpy8I GTNNAC 8 cut(s) 265, 525, 1075, 1403, 1809, 2243, 2584, 2692
Hpy99I CGWCG 4 cut(s) 49, 77, 92, 197
HpyCH4III ACNGT 7 cut(s) 558, 1381, 1699, 1771, 1880, 2276, 2560
HpyCH4IV ACGT 4 cut(s) 195, 228, 967, 1001
HpyF10VI GCNNNNNNNGC 8 cut(s) 179, 380, 485, 508, 596, 599, 1628, 2345
HpyF3I CTNAG 8 cut(s) 35, 213, 237, 495, 798, 2025, 2205, 2601
HpySE526I ACGT 4 cut(s) 195, 228, 967, 1001
Hsp92I GRCGYC 4 cut(s) 18, 44, 75, 195
HspAI GCGC 6 cut(s) 18, 374, 413, 477, 721, 2348
KasI GGCGCC 1 cut(s) 17
Kpn2I TCCGGA 1 cut(s) 60
Ksp22I TGATCA 3 cut(s) 1801, 2035, 2188
LguI GCTCTTC 1 cut(s) 2955
LmnI GCTCC 6 cut(s) 118, 587, 1035, 2092, 2366, 2756
LweI GCATC 7 cut(s) 117, 784, 2245, 2275, 2326, 2914, 2956
MaeI CTAG 6 cut(s) 341, 447, 1058, 2166, 2496, 2783
MaeII ACGT 4 cut(s) 195, 228, 967, 1001
MflI RGATCY 8 cut(s) 337, 346, 393, 451, 711, 1351, 1555, 2486
MhlI GDGCHC 6 cut(s) 1405, 1620, 2031, 2416, 2456, 2950
MlsI TGGCCA 1 cut(s) 1847
MluNI TGGCCA 1 cut(s) 1847
Mly113I GGCGCC 1 cut(s) 18
MlyI GAGTC 7 cut(s) 131, 229, 872, 2169, 2216, 2844, 2993
MmeI TCCRAC 4 cut(s) 565, 1719, 2409, 2497
Mox20I TGGCCA 1 cut(s) 1847
MroI TCCGGA 1 cut(s) 60
MroXI GAANNNNTTC 2 cut(s) 806, 2103
MscI TGGCCA 1 cut(s) 1847
MslI CAYNNNNRTG 2 cut(s) 1030, 2253
Msp20I TGGCCA 1 cut(s) 1847
MspA1I CMGCKG 2 cut(s) 599, 1064
MspI CCGG 4 cut(s) 61, 390, 397, 2041
MspR9I CCNGG 5 cut(s) 391, 2055, 2091, 2442, 2729
Mva1269I GAATGC 1 cut(s) 3014
MvaI CCWGG 4 cut(s) 2055, 2091, 2442, 2729
MwoI GCNNNNNNNGC 8 cut(s) 179, 380, 485, 508, 596, 599, 1628, 2345
NarI GGCGCC 1 cut(s) 18
NciI CCSGG 1 cut(s) 391
NcoI CCATGG 1 cut(s) 384
NdeI CATATG 1 cut(s) 1238
NlaIV GGNNCC 7 cut(s) 19, 70, 348, 395, 713, 844, 1617
NmuCI GTSAC 6 cut(s) 1086, 1874, 1953, 2161, 2270, 2985
NsbI TGCGCA 1 cut(s) 2349
NspI RCATGY 3 cut(s) 407, 1434, 1790
PaeI GCATGC 1 cut(s) 1790
PagI TCATGA 2 cut(s) 859, 936
PceI AGGCCT 1 cut(s) 2923
PciSI GCTCTTC 1 cut(s) 2955
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PctI GAATGC 1 cut(s) 3014
PdmI GAANNNNTTC 2 cut(s) 806, 2103
PflFI GACNNNGTC 1 cut(s) 238
PinAI ACCGGT 1 cut(s) 2040
PleI GAGTC 7 cut(s) 131, 229, 871, 2168, 2215, 2843, 2992
PluTI GGCGCC 1 cut(s) 21
PmaCI CACGTG 1 cut(s) 968
PmlI CACGTG 1 cut(s) 968
PpsI GAGTC 7 cut(s) 131, 229, 871, 2168, 2215, 2843, 2992
Ppu21I YACGTR 1 cut(s) 968
PshAI GACNNNNGTC 1 cut(s) 2834
PshBI ATTAAT 1 cut(s) 321
Psp124BI GAGCTC 3 cut(s) 2416, 2456, 2950
Psp6I CCWGG 4 cut(s) 2053, 2089, 2440, 2727
PspCI CACGTG 1 cut(s) 968
PspGI CCWGG 4 cut(s) 2053, 2089, 2440, 2727
PspN4I GGNNCC 7 cut(s) 19, 70, 348, 395, 713, 844, 1617
PspPI GGNCC 1 cut(s) 905
PstI CTGCAG 1 cut(s) 598
PstNI CAGNNNCTG 4 cut(s) 244, 1067, 2204, 2339
PsuI RGATCY 8 cut(s) 337, 346, 393, 451, 711, 1351, 1555, 2486
PsyI GACNNNGTC 1 cut(s) 238
PvuII CAGCTG 2 cut(s) 599, 1064
RsaI GTAC 7 cut(s) 266, 272, 1018, 1056, 1701, 2076, 2119
RsaNI GTAC 7 cut(s) 265, 271, 1017, 1055, 1700, 2075, 2118
RseI CAYNNNNRTG 2 cut(s) 1030, 2253
SacI GAGCTC 3 cut(s) 2416, 2456, 2950
SapI GCTCTTC 1 cut(s) 2955
Sau96I GGNCC 1 cut(s) 905
ScaI AGTACT 2 cut(s) 1701, 2076
SchI GAGTC 7 cut(s) 131, 229, 872, 2169, 2216, 2844, 2993
ScrFI CCNGG 5 cut(s) 391, 2055, 2091, 2442, 2729
SduI GDGCHC 6 cut(s) 1405, 1620, 2031, 2416, 2456, 2950
SfaNI GCATC 7 cut(s) 117, 784, 2245, 2275, 2326, 2914, 2956
SfcI CTRYAG 3 cut(s) 594, 1695, 2790
SfoI GGCGCC 1 cut(s) 19
SmiMI CAYNNNNRTG 2 cut(s) 1030, 2253
SmlI CTYRAG 2 cut(s) 1703, 1923
SmoI CTYRAG 2 cut(s) 1703, 1923
SpeI ACTAGT 1 cut(s) 2165
SphI GCATGC 1 cut(s) 1790
SseBI AGGCCT 1 cut(s) 2923
SsiI CCGC 3 cut(s) 24, 511, 1620
SspDI GGCGCC 1 cut(s) 17
SspI AATATT 2 cut(s) 917, 1510
SspMI CTAG 6 cut(s) 341, 447, 1058, 2166, 2496, 2783
SstI GAGCTC 3 cut(s) 2416, 2456, 2950
StuI AGGCCT 1 cut(s) 2923
StyD4I CCNGG 5 cut(s) 389, 2053, 2089, 2440, 2727
StyI CCWWGG 3 cut(s) 184, 384, 1682
TaaI ACNGT 7 cut(s) 558, 1381, 1699, 1771, 1880, 2276, 2560
TaiI ACGT 4 cut(s) 198, 231, 970, 1004
TaqI TCGA 8 cut(s) 123, 135, 192, 317, 516, 681, 889, 2450
TatI WGTACW 5 cut(s) 264, 1054, 1699, 2074, 2117
TauI GCSGC 1 cut(s) 514
TscAI CASTG 6 cut(s) 1082, 1384, 2266, 2272, 2281, 2563
TseFI GTSAC 6 cut(s) 1086, 1874, 1953, 2161, 2270, 2985
Tsp45I GTSAC 6 cut(s) 1086, 1874, 1953, 2161, 2270, 2985
TspGWI ACGGA 3 cut(s) 444, 1962, 2307
TspRI CASTG 6 cut(s) 1082, 1384, 2266, 2272, 2281, 2563
Tth111I GACNNNGTC 1 cut(s) 238
VneI GTGCAC 1 cut(s) 1401
VspI ATTAAT 1 cut(s) 321
XceI RCATGY 3 cut(s) 407, 1434, 1790
XmiI GTMKAC 1 cut(s) 524
XmnI GAANNNNTTC 2 cut(s) 806, 2103
XspI CTAG 6 cut(s) 341, 447, 1058, 2166, 2496, 2783
ZraI GACGTC 1 cut(s) 196
ZrmI AGTACT 2 cut(s) 1701, 2076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.