Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009227

Physical Location & Seq

Genomic Coordinates Reverse (-)

27812816 .. 27817873

Gene Structure 5058 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1200100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3201 bp

ATGGTGTTCCGGATCGGCACCGACGCGCACCTCTACGACGATCCCGACGACGTCAGCATCGCTCCTCTCCTCGACAGCAGGTTCGACTCCGAGAAGTGCGAGGCTCTCAAGCGATTGCTCGCTCTCATCGCCCAGGGCTTCGAAGTCTCCAATTTCTTCCCTCAGGTTGTGAAGAATGTAGCGTCGCAGTCGTTGGAAGTGAAGAAGCTCGTTTACTTGTATTTGTTGCATTATGCTCAAAAGCGTCCAAACGAAGCATTGCTGTCGATTAATTATTTCCAGAAGGATTTAGGGGATCCGAATCCGTTAGTGAGGGCGTGGGCACTGAGAACAATGGCAGGGATTCGGCTACACGTTATTGCACCTTTGGTTCTTGTTGCAGCAGGAAAATGTGCTAGAGATCCCTCTGTGTATGTTAGAAAATGCGCGGCCAATGCACTTCGTAAGCTGCACGATTTGCACCTTGAGGAGAATACTGCGGGGATTGAAGAGATTATTGGAATATTGTTGAATGACTTCCCCTTGTGTTGTTGGAGCTGCTGCTGCTGCTTTCTCTTCTGTTTGTTATGCGAGGTTCTTCCCGATGTGGAAGAATGGGGTAAAATAGTCTTAATTGGAATCCTTTTGCGCTATGTAGTTGCAAGGCATGGGCTTGTAAAGGATTCCATTATGTTTTCTTTGCATGGAACTGAGAATTCTCGATCTGAAAAGTATTGCTTGGATAACAACTCTGCGTTGGATGACAACGGTGACATGAATGATCTTTATGAGTCTGAATTAACAAATGCTGTATCCCGTTGTTATATTGAAGGACCAGCTGAATATTTATCGCGATTAAGTTTCATGAATAAAGATTCCTCTGAATTTAGTTGTGCTCGTTTTACATCTGGAAAAAATAATGATGATGTGAAGATCCTGCTGCAATGTACATCGCCATTGTTGTGGAGTAATAACAGTGCAGTTGTACTAGCCGCTGCTGGCGTACATTGGATTATGGCGCCGGTGGAGGATTTGAAAAGAATCGTTAAGCCACTCTTGTTCGTCCTAAGATCATCTAATGCCTCAAAATATGTGGTTTTATGTAACATTCAAGTGTTGGCCAAAGCAATTCCTTCACTCTTCTCTCCATACTTTGAAGATTTCTTTATATGCTCTTTGGATTCATATCAAATTAAAGCTTTGAAACTGGATATACTGGCTTACATTGCTACAGATTCATCAATTTCATTTATACTCAAAGAATTTCAGGATTATATTAGAGACCCAGACAGGAGATTTGCTGCTGATACTGTTGCTAGAATTGGAATATGTGCGCAACGACTTCCAGAAATGTCAAATTCATGCTTGGAGTTTCTATTGGCTTTGACCAGACAGCAACTTACAACAGGTGAGTTTGGGTCTGTGGATGCTGAAGCAGATATACTGATTCAAGCAATAATGTCCATAAAGTCAATCATTCAGCAAGATCCACCCAGTCATGAAAAGGTTATTATCCAATTGGTTCGAAGTTTAACTTCAATAAAGGTGCCTGATGTGCGTGCTATGGTTGTTTGGATGGTGGGGGAGTATAACTCTTTAGGTGATCTCATCCCAAGGATGTTAGCTACAGTGCTCAAGTATCTTGCAAGGTGCTTTGCTTCCGAAGAAGTGGAAACAAAGCTTCAGATTTGTAATACCGCTGTTAAGGTTTTATCAGGGACAAAAGGGAATGACCTGTTGACAATCAAAATGGTTTTAATCTACGTGTTGGAGTTGGCCAAATGCGATTTAAACTATGACATTTGTGACCGAGCTCATTTCTTAAAGAAACTTCTTTCAACTTATCTAGATTCTCAAGGCTTGGAGGAGGAAAACAACCGTCCGCCCCAAGATAAAGATAGTTCACGTCTCCTGGCAGAAATCTTGTTTGGAAAACAAAGGAAACCAGTGTCCCATGAGCCCATTGATCAACGGTTTTACCTTCCTGGCTCTTTATCGCAGATAGTACTTCATGCTGCTCCTGGGTACGAACCTCTCCCAAAGCCTTGTAGTTTGCGCAGTGATGGCCCCAAACTCAATGAATTTGGAGAGGCAGTCACTAATGGTGACCAATATGTTACAGATGATGAGGACTCAGCTGGTTCTGCACGTGAAGATGATGGTAATTCTCATCAGTTGATTCAGTTTTCAGATAAAAGGACAAATGGAGCTTCTCAGTCTGCTTCTGATTTTGGGGAGTTGTTGTCAAATAGAGCTTTAGAATCATGGTTAGATGACCAACCTGGTTTTTCAAACCCAAATACTTCAGAACAAAGTCAAGTCCGTACGTCTTCAGCAAGAATCTCCATCAGGGACATCGGAGGTCAAGTTAGACATAAAAGCTATCCACTCTTAGACCCTGTGAATGGAAACGGCTTAAAGGTTGACTATTCGTTTTCATCTGAGATATCAAGTATCTCTCCTCTATTTATATGTATAGAAGTTTCCTTCAAGAACTGTTCAAAGGAGACCGTGTCTGATGTGACTTTGGTTGACGAGGAGGCTGGCAAAGGCAAGGATTCTGTAGACCAAGCTTCGTTTTCAGATGAAAGCTCAACGATACCTCAAAATAATGAGCCAAATCTGGTTTCTATAGAAGAAATCACTTCTCTGGAACCTGGCCAGACTGTGACAAGAAGCATCCAGGTCCGCTTCCATCACCACCTCTTGCCTCTAAAACTGACCTTATATTGTAACGGCAAGAAGCATCCTATTAAATTGCGGCCTGACATTGGATACATTGTAAAAGCTCTTCCTATGGATGTTGAAGCCTTTACAAATAAGGAGTCTCAACTGCGTGGAATGTTTGAATGCGTAAGAAGGTGCACCTTCACTGATCACATCAAGGAGTTCGACAAGGACAACTCATTGGTAGAAGACAAATTTCTCGTAATCTGTCAAAGTCTTGCATTGAAGATGCTTAGCAATGCAAATCTTCATCTTGTATCCATCGATTTGCCAGTTGCAGCGAAGCTTGATGATGCAACAGGTTTGTGCTTACGATTCAGCAGCAAGATTTTGAGCACCTCTGTCCCCTGCTTGATAACCGTTACTGTCAAAGGTAGATGTACAGAACCATTGGAAATATCAGTTAAAGTGAACTGTGAAGAAACTGTATTTGGGTTGAATCTTCTGAACAGGATCGTAAATTTCTCAGTCGAACCCTCTCATGCCCATAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04142 map04142

Pfam Domains

Protein Families

AP3B1_C Adaptin_N

Protein Analysis

1067

Amino Acids

118.85

Weight (kDa)

5.44

Isoelectric Point (pI)

40.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Adaptin_N	PF01602	23 - 607	2.3e-79	Adaptin N terminal region
AP3B1_C	PF14796	758 - 842	1.5e-08	Clathrin-adaptor complex-3 beta-1 subunit C-terminal
AP3B1_C_2	PF24080	920 - 1056	1.1e-07	AP-3 complex subunit beta-1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55480 AT3G55480
fragaria_vesca	FvH4_7g22680 FvH4_7g22680 FvH4_7g22680 FvH4_7g22680
malus_domestica	MD01G1135400.v1.1 MD07G1200100.v1.1
prunus_persica	Prupe.2G236800_v2.0.a1 Prupe.2G236800_v2.0.a1
pyrus_communis	pycom01g15610 pycom07g18690
rosa_chinensis	RchiOBHm_Chr1g0367761
rosa_laevigata	RLG00000027265
rosa_multiflora	Rmu_ssc0000395.1_g000020
rosa_roxburghii	Rroxscaffold_4G00289240
rosa_rugosa	Rorug01G0336400
rosa_samantha	Rh1AG343600 Rh1BG305500 Rh1CG321100 Rh1DG337700
rosa_wichuraiana	Rw1G030580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	54
Acc16I	TGCGCA	2 cut(s)	1314, 2036
Acc36I	ACCTGC	1 cut(s)	69
AccB1I	GGYRCC	3 cut(s)	17, 997, 1525
AccI	GTMKAC	1 cut(s)	2544
AccII	CGCG	3 cut(s)	26, 428, 832
AccIII	TCCGGA	1 cut(s)	9
AciI	CCGC	7 cut(s)	428, 479, 972, 1677, 1862, 2668, 2740
AclWI	GGATC	8 cut(s)	20, 35, 290, 303, 395, 907, 1460, 3167
AcoI	YGGCCR	4 cut(s)	429, 1098, 1755, 2638
AcsI	RAATTY	7 cut(s)	694, 863, 1241, 1336, 2060, 2900, 3166
AcuI	CTGAAG	4 cut(s)	1431, 1646, 2268, 2295
AcvI	CACGTG	1 cut(s)	2129
AcyI	GRCGYC	2 cut(s)	51, 998
AfaI	GTAC	7 cut(s)	928, 966, 984, 1986, 2006, 2305, 3088
AfiI	CCNNNNNNNGG	2 cut(s)	2384, 2750
AflIII	ACRYGT	2 cut(s)	352, 1743
AjiI	CACGTC	1 cut(s)	1886
AjnI	CCWGG	7 cut(s)	132, 1890, 1963, 1999, 2260, 2635, 2661
AjuI	GAANNNNNNNTTGG	8 cut(s)	480, 512, 1890, 1922, 2590, 2622, 3120, 3152
Alw21I	GWGCWC	5 cut(s)	877, 1614, 1795, 2846, 3044
Alw26I	GTCTC	5 cut(s)	151, 1254, 1892, 2480, 2811
Alw44I	GTGCAC	1 cut(s)	2842
AlwI	GGATC	8 cut(s)	20, 35, 290, 303, 395, 907, 1460, 3167
Aor13HI	TCCGGA	1 cut(s)	9
AoxI	GGCC	6 cut(s)	429, 1098, 1755, 2044, 2638, 2741
ApaLI	GTGCAC	1 cut(s)	2842
ApoI	RAATTY	7 cut(s)	694, 863, 1241, 1336, 2060, 2900, 3166
ArsI	GACNNNNNNTTYG	6 cut(s)	65, 97, 1259, 1291, 2789, 2821
AseI	ATTAAT	1 cut(s)	270
Asp700I	GAANNNNTTC	1 cut(s)	515
AspLEI	GCGC	6 cut(s)	28, 428, 630, 1000, 1315, 2037
AspS9I	GGNCC	3 cut(s)	812, 2045, 2665
AsuHPI	GGTGA	5 cut(s)	761, 1400, 1592, 2096, 2669
AsuII	TTCGAA	2 cut(s)	141, 1504
AvaII	GGWCC	2 cut(s)	812, 2665
AxyI	CCTNAGG	1 cut(s)	162
BaeGI	GKGCMC	2 cut(s)	325, 2846
BalI	TGGCCA	3 cut(s)	1100, 1757, 2640
BamHI	GGATCC	1 cut(s)	295
BanI	GGYRCC	3 cut(s)	17, 997, 1525
BanII	GRGCYC	2 cut(s)	1795, 1941
BarI	GAAGNNNNNNTAC	6 cut(s)	197, 229, 1404, 1436, 2754, 2786
BbrPI	CACGTG	1 cut(s)	2129
BbsI	GAAGAC	2 cut(s)	2301, 2901
Bbv12I	GWGCWC	5 cut(s)	877, 1614, 1795, 2846, 3044
BccI	CCATC	6 cut(s)	1549, 2036, 2132, 2333, 2682, 2975
BceAI	ACGGC	2 cut(s)	2407, 2731
BcgI	CGANNNNNNTGC	2 cut(s)	246, 280
BciT130I	CCWGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
BciVI	GTATCC	3 cut(s)	802, 2747, 2974
BclI	TGATCA	2 cut(s)	1945, 2854
BcoDI	GTCTC	5 cut(s)	151, 1254, 1892, 2480, 2811
BfaI	CTAG	4 cut(s)	396, 968, 1296, 1826
BfmI	CTRYAG	4 cut(s)	1209, 1605, 2541, 2610
BfoI	RGCGCY	1 cut(s)	1001
BfuAI	ACCTGC	1 cut(s)	69
BfuI	GTATCC	3 cut(s)	802, 2747, 2974
BlpI	GCTNAGC	1 cut(s)	2939
BmcAI	AGTACT	1 cut(s)	1986
Bme1390I	CCNGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
Bme18I	GGWCC	2 cut(s)	812, 2665
BmgBI	CACGTC	1 cut(s)	1886
BmgT120I	GGNCC	3 cut(s)	812, 2045, 2665
BmiI	GGNNCC	6 cut(s)	19, 297, 999, 1527, 2047, 2634
BmrFI	CCNGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
BmrI	ACTGGG	1 cut(s)	1467
BmsI	GCATC	6 cut(s)	66, 1396, 2667, 2734, 2925, 2989
BmuI	ACTGGG	1 cut(s)	1467
BpiI	GAAGAC	2 cut(s)	2301, 2901
BplI	GAGNNNNNCTC	2 cut(s)	1556, 1588
Bpu1102I	GCTNAGC	1 cut(s)	2939
Bpu14I	TTCGAA	2 cut(s)	141, 1504
BpuEI	CTTGAG	4 cut(s)	92, 485, 1598, 1818
Bsa29I	ATCGAT	1 cut(s)	2970
BsaAI	YACGTR	2 cut(s)	1744, 2129
BsaBI	GATNNNNATC	2 cut(s)	300, 1164
BsaHI	GRCGYC	2 cut(s)	51, 998
BsaI	GGTCTC	2 cut(s)	1254, 2480
BsaJI	CCNNGG	4 cut(s)	132, 133, 1592, 2000
BsaWI	WCCGGW	1 cut(s)	9
BsaXI	ACNNNNNCTCC	8 cut(s)	2058, 2088, 2207, 2237, 2331, 2361, 2477, 2507
Bsc4I	CCNNNNNNNGG	2 cut(s)	2384, 2750
Bse118I	RCCGGY	1 cut(s)	1000
Bse1I	ACTGG	5 cut(s)	1191, 1200, 1473, 1925, 2978
Bse21I	CCTNAGG	1 cut(s)	162
Bse3DI	GCAATG	4 cut(s)	257, 929, 1203, 2950
Bse8I	GATNNNNATC	2 cut(s)	300, 1164
BseAI	TCCGGA	1 cut(s)	9
BseBI	CCWGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
BseCI	ATCGAT	1 cut(s)	2970
BseDI	CCNNGG	4 cut(s)	132, 133, 1592, 2000
BseGI	GGATG	8 cut(s)	745, 1411, 1560, 1587, 1602, 2658, 2725, 2785
BseJI	GATNNNNATC	2 cut(s)	300, 1164
BseLI	CCNNNNNNNGG	2 cut(s)	2384, 2750
BseMI	GCAATG	4 cut(s)	257, 929, 1203, 2950
BseMII	CTCAG	7 cut(s)	176, 317, 681, 2127, 2207, 2412, 3186
BseNI	ACTGG	5 cut(s)	1191, 1200, 1473, 1925, 2978
BseRI	GAGGAG	6 cut(s)	54, 59, 482, 1859, 2430, 2531
BseSI	GKGCMC	2 cut(s)	325, 2846
BsgI	GTGCAG	3 cut(s)	434, 978, 2109
Bsh1236I	CGCG	3 cut(s)	26, 428, 832
BshFI	GGCC	6 cut(s)	431, 1100, 1757, 2046, 2640, 2743
BshNI	GGYRCC	3 cut(s)	17, 997, 1525
BshVI	ATCGAT	1 cut(s)	2970
BsiHKAI	GWGCWC	5 cut(s)	877, 1614, 1795, 2846, 3044
BsiSI	CCGG	2 cut(s)	10, 1001
BsiWI	CGTACG	1 cut(s)	2303
BslFI	GGGAC	4 cut(s)	1711, 1915, 2345, 3035
BslI	CCNNNNNNNGG	2 cut(s)	2384, 2750
BsmAI	GTCTC	5 cut(s)	151, 1254, 1892, 2480, 2811
BsmBI	CGTCTC	1 cut(s)	1892
BsmFI	GGGAC	4 cut(s)	1711, 1915, 2345, 3035
BsmI	GAATGC	1 cut(s)	2834
BsnI	GGCC	6 cut(s)	431, 1100, 1757, 2046, 2640, 2743
Bso31I	GGTCTC	2 cut(s)	1254, 2480
Bsp119I	TTCGAA	2 cut(s)	141, 1504
Bsp1286I	GDGCHC	7 cut(s)	325, 877, 1614, 1795, 1941, 2846, 3044
Bsp13I	TCCGGA	1 cut(s)	9
Bsp1407I	TGTACA	2 cut(s)	926, 3086
Bsp1720I	GCTNAGC	1 cut(s)	2939
Bsp68I	TCGCGA	1 cut(s)	832
BspACI	CCGC	7 cut(s)	428, 479, 972, 1677, 1862, 2668, 2740
BspANI	GGCC	6 cut(s)	431, 1100, 1757, 2046, 2640, 2743
BspCNI	CTCAG	7 cut(s)	175, 318, 682, 2126, 2206, 2413, 3185
BspDI	ATCGAT	1 cut(s)	2970
BspEI	TCCGGA	1 cut(s)	9
BspFNI	CGCG	3 cut(s)	26, 428, 832
BspHI	TCATGA	2 cut(s)	843, 1477
BspLI	GGNNCC	6 cut(s)	19, 297, 999, 1527, 2047, 2634
BspMI	ACCTGC	1 cut(s)	69
BspPI	GGATC	8 cut(s)	20, 35, 290, 303, 395, 907, 1460, 3167
BspQI	GCTCTTC	1 cut(s)	2775
BspT104I	TTCGAA	2 cut(s)	141, 1504
BspT107I	GGYRCC	3 cut(s)	17, 997, 1525
BspTNI	GGTCTC	2 cut(s)	1254, 2480
BsrDI	GCAATG	4 cut(s)	257, 929, 1203, 2950
BsrFI	RCCGGY	1 cut(s)	1000
BsrGI	TGTACA	2 cut(s)	926, 3086
BsrI	ACTGG	5 cut(s)	1191, 1200, 1473, 1925, 2978
BssAI	RCCGGY	1 cut(s)	1000
BssECI	CCNNGG	4 cut(s)	132, 133, 1592, 2000
BssNI	GRCGYC	2 cut(s)	51, 998
BssT1I	CCWWGG	1 cut(s)	1592
Bst2UI	CCWGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
Bst6I	CTCTTC	4 cut(s)	483, 560, 1124, 2775
BstACI	GRCGYC	2 cut(s)	51, 998
BstAPI	GCANNNNNTGC	1 cut(s)	457
BstAUI	TGTACA	2 cut(s)	926, 3086
BstBAI	YACGTR	2 cut(s)	1744, 2129
BstBI	TTCGAA	2 cut(s)	141, 1504
BstC8I	GCNNGC	4 cut(s)	120, 979, 1539, 2524
BstEII	GGTNACC	1 cut(s)	2084
BstF5I	GGATG	8 cut(s)	745, 1411, 1560, 1587, 1602, 2658, 2725, 2785
BstFNI	CGCG	3 cut(s)	26, 428, 832
BstH2I	RGCGCY	1 cut(s)	1001
BstHHI	GCGC	6 cut(s)	28, 428, 630, 1000, 1315, 2037
BstMAI	GTCTC	5 cut(s)	151, 1254, 1892, 2480, 2811
BstMWI	GCNNNNNNNGC	9 cut(s)	128, 434, 457, 543, 546, 1205, 1534, 2043, 2123
BstNI	CCWGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
BstPI	GGTNACC	1 cut(s)	2084
BstSCI	CCNGG	7 cut(s)	132, 1890, 1963, 1999, 2260, 2635, 2661
BstSFI	CTRYAG	4 cut(s)	1209, 1605, 2541, 2610
BstSLI	GKGCMC	2 cut(s)	325, 2846
BstUI	CGCG	3 cut(s)	26, 428, 832
BstV2I	GAAGAC	2 cut(s)	2301, 2901
BstX2I	RGATCY	4 cut(s)	295, 400, 912, 1465
BstXI	CCANNNNNNTGG	1 cut(s)	942
BstYI	RGATCY	4 cut(s)	295, 400, 912, 1465
Bsu15I	ATCGAT	1 cut(s)	2970
Bsu36I	CCTNAGG	1 cut(s)	162
BsuI	GTATCC	3 cut(s)	802, 2747, 2974
BsuRI	GGCC	6 cut(s)	431, 1100, 1757, 2046, 2640, 2743
BsuTUI	ATCGAT	1 cut(s)	2970
BtgZI	GCGATG	3 cut(s)	43, 112, 915
BtrI	CACGTC	1 cut(s)	1886
BtsCI	GGATG	8 cut(s)	745, 1411, 1560, 1587, 1602, 2658, 2725, 2785
BtsI	GCAGTG	1 cut(s)	2044
BtsIMutI	CAGTG	6 cut(s)	323, 961, 1614, 1932, 2044, 2850
BtuMI	TCGCGA	1 cut(s)	832
BveI	ACCTGC	1 cut(s)	69
Cac8I	GCNNGC	4 cut(s)	120, 979, 1539, 2524
CciI	TCATGA	2 cut(s)	843, 1477
CfoI	GCGC	6 cut(s)	28, 428, 630, 1000, 1315, 2037
Cfr10I	RCCGGY	1 cut(s)	1000
Cfr13I	GGNCC	3 cut(s)	812, 2045, 2665
ClaI	ATCGAT	1 cut(s)	2970
CseI	GACGC	3 cut(s)	32, 171, 233
CsiI	ACCWGGT	1 cut(s)	2260
Csp6I	GTAC	7 cut(s)	927, 965, 983, 1985, 2005, 2304, 3087
CspCI	CAANNNNNGTGG	2 cut(s)	1053, 1088
CviQI	GTAC	7 cut(s)	927, 965, 983, 1985, 2005, 2304, 3087
DinI	GGCGCC	1 cut(s)	999
DraI	TTTAAA	1 cut(s)	1770
EaeI	YGGCCR	4 cut(s)	429, 1098, 1755, 2638
Eam1104I	CTCTTC	4 cut(s)	483, 560, 1124, 2775
EarI	CTCTTC	4 cut(s)	483, 560, 1124, 2775
EciI	GGCGGA	1 cut(s)	1851
Ecl136II	GAGCTC	1 cut(s)	1793
Eco130I	CCWWGG	1 cut(s)	1592
Eco24I	GRGCYC	2 cut(s)	1795, 1941
Eco31I	GGTCTC	2 cut(s)	1254, 2480
Eco32I	GATATC	1 cut(s)	2427
Eco47I	GGWCC	2 cut(s)	812, 2665
Eco53kI	GAGCTC	1 cut(s)	1793
Eco57I	CTGAAG	4 cut(s)	1431, 1646, 2268, 2295
Eco72I	CACGTG	1 cut(s)	2129
Eco81I	CCTNAGG	1 cut(s)	162
Eco91I	GGTNACC	1 cut(s)	2084
EcoICRI	GAGCTC	1 cut(s)	1793
EcoO65I	GGTNACC	1 cut(s)	2084
EcoRI	GAATTC	1 cut(s)	694
EcoRII	CCWGG	7 cut(s)	132, 1890, 1963, 1999, 2260, 2635, 2661
EcoRV	GATATC	1 cut(s)	2427
EcoT14I	CCWWGG	1 cut(s)	1592
EcoT38I	GRGCYC	2 cut(s)	1795, 1941
EgeI	GGCGCC	1 cut(s)	999
EheI	GGCGCC	1 cut(s)	999
ErhI	CCWWGG	1 cut(s)	1592
Esp3I	CGTCTC	1 cut(s)	1892
FalI	AAGNNNNNCTT	6 cut(s)	701, 733, 1019, 1051, 1498, 1530
FaqI	GGGAC	4 cut(s)	1711, 1915, 2345, 3035
FauI	CCCGC	1 cut(s)	472
FbaI	TGATCA	2 cut(s)	1945, 2854
FblI	GTMKAC	1 cut(s)	2544
FokI	GGATG	8 cut(s)	752, 1418, 1567, 1574, 1609, 2645, 2712, 2792
FriOI	GRGCYC	2 cut(s)	1795, 1941
FspBI	CTAG	4 cut(s)	396, 968, 1296, 1826
FspI	TGCGCA	2 cut(s)	1314, 2036
GlaI	GCGC	6 cut(s)	27, 427, 629, 999, 1314, 2036
HaeII	RGCGCY	1 cut(s)	1001
HaeIII	GGCC	6 cut(s)	431, 1100, 1757, 2046, 2640, 2743
HapII	CCGG	2 cut(s)	10, 1001
HgaI	GACGC	3 cut(s)	32, 171, 233
HhaI	GCGC	6 cut(s)	28, 428, 630, 1000, 1315, 2037
Hin1I	GRCGYC	2 cut(s)	51, 998
Hin6I	GCGC	6 cut(s)	26, 426, 628, 998, 1313, 2035
HinP1I	GCGC	6 cut(s)	26, 426, 628, 998, 1313, 2035
HincII	GTYRAC	3 cut(s)	1719, 2404, 2512
HindII	GTYRAC	3 cut(s)	1719, 2404, 2512
HindIII	AAGCTT	4 cut(s)	1176, 1658, 2550, 2990
HpaII	CCGG	2 cut(s)	10, 1001
HphI	GGTGA	5 cut(s)	761, 1400, 1592, 2096, 2669
Hpy166II	GTNNAC	8 cut(s)	214, 1719, 1883, 2404, 2512, 2545, 2844, 3118
Hpy8I	GTNNAC	8 cut(s)	214, 1719, 1883, 2404, 2512, 2545, 2844, 3118
Hpy99I	CGWCG	5 cut(s)	26, 41, 50, 53, 187
HpyAV	CCTTC	7 cut(s)	277, 803, 1122, 1970, 2476, 2832, 2857
HpyCH4IV	ACGT	6 cut(s)	51, 354, 1743, 1885, 2128, 2306
HpyF10VI	GCNNNNNNNGC	9 cut(s)	128, 434, 457, 543, 546, 1205, 1534, 2043, 2123
HpySE526I	ACGT	6 cut(s)	51, 354, 1743, 1885, 2128, 2306
Hsp92I	GRCGYC	2 cut(s)	51, 998
HspAI	GCGC	6 cut(s)	26, 426, 628, 998, 1313, 2035
KasI	GGCGCC	1 cut(s)	997
Kpn2I	TCCGGA	1 cut(s)	9
Ksp22I	TGATCA	2 cut(s)	1945, 2854
LguI	GCTCTTC	1 cut(s)	2775
LmnI	GCTCC	4 cut(s)	67, 534, 2002, 2186
LweI	GCATC	6 cut(s)	66, 1396, 2667, 2734, 2925, 2989
MabI	ACCWGGT	1 cut(s)	2260
MaeI	CTAG	4 cut(s)	396, 968, 1296, 1826
MaeII	ACGT	6 cut(s)	51, 354, 1743, 1885, 2128, 2306
MfeI	CAATTG	1 cut(s)	1496
MflI	RGATCY	4 cut(s)	295, 400, 912, 1465
MhlI	GDGCHC	7 cut(s)	325, 877, 1614, 1795, 1941, 2846, 3044
MlsI	TGGCCA	3 cut(s)	1100, 1757, 2640
MluNI	TGGCCA	3 cut(s)	1100, 1757, 2640
Mly113I	GGCGCC	1 cut(s)	998
MlyI	GAGTC	4 cut(s)	80, 779, 2105, 2813
MmeI	TCCRAC	4 cut(s)	174, 512, 717, 1728
Mox20I	TGGCCA	3 cut(s)	1100, 1757, 2640
MroI	TCCGGA	1 cut(s)	9
MroXI	GAANNNNTTC	1 cut(s)	515
MscI	TGGCCA	3 cut(s)	1100, 1757, 2640
MslI	CAYNNNNRTG	2 cut(s)	940, 1091
Msp20I	TGGCCA	3 cut(s)	1100, 1757, 2640
MspA1I	CMGCKG	4 cut(s)	818, 974, 1679, 2117
MspI	CCGG	2 cut(s)	10, 1001
MspR9I	CCNGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
MunI	CAATTG	1 cut(s)	1496
Mva1269I	GAATGC	1 cut(s)	2834
MvaI	CCWGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
MvnI	CGCG	3 cut(s)	26, 428, 832
MwoI	GCNNNNNNNGC	9 cut(s)	128, 434, 457, 543, 546, 1205, 1534, 2043, 2123
NarI	GGCGCC	1 cut(s)	998
NlaIV	GGNNCC	6 cut(s)	19, 297, 999, 1527, 2047, 2634
NmuCI	GTSAC	6 cut(s)	749, 1784, 2074, 2084, 2500, 2647
NruI	TCGCGA	1 cut(s)	832
NsbI	TGCGCA	2 cut(s)	1314, 2036
NspV	TTCGAA	2 cut(s)	141, 1504
PagI	TCATGA	2 cut(s)	843, 1477
PasI	CCCWGGG	1 cut(s)	133
PciSI	GCTCTTC	1 cut(s)	2775
PcsI	WCGNNNNNNNCGW	2 cut(s)	42, 45
PctI	GAATGC	1 cut(s)	2834
PdmI	GAANNNNTTC	1 cut(s)	515
Pfl23II	CGTACG	1 cut(s)	2303
PflFI	GACNNNGTC	2 cut(s)	50, 2491
PleI	GAGTC	4 cut(s)	80, 778, 2105, 2812
PluTI	GGCGCC	1 cut(s)	1001
PmaCI	CACGTG	1 cut(s)	2129
PmlI	CACGTG	1 cut(s)	2129
PpsI	GAGTC	4 cut(s)	80, 778, 2105, 2812
Ppu21I	YACGTR	2 cut(s)	1744, 2129
PshBI	ATTAAT	1 cut(s)	270
Psp124BI	GAGCTC	1 cut(s)	1795
Psp6I	CCWGG	7 cut(s)	132, 1890, 1963, 1999, 2260, 2635, 2661
PspCI	CACGTG	1 cut(s)	2129
PspEI	GGTNACC	1 cut(s)	2084
PspGI	CCWGG	7 cut(s)	132, 1890, 1963, 1999, 2260, 2635, 2661
PspLI	CGTACG	1 cut(s)	2303
PspN4I	GGNNCC	6 cut(s)	19, 297, 999, 1527, 2047, 2634
PspPI	GGNCC	3 cut(s)	812, 2045, 2665
PsuI	RGATCY	4 cut(s)	295, 400, 912, 1465
PsyI	GACNNNGTC	2 cut(s)	50, 2491
PvuII	CAGCTG	2 cut(s)	818, 2117
RruI	TCGCGA	1 cut(s)	832
RsaI	GTAC	7 cut(s)	928, 966, 984, 1986, 2006, 2305, 3088
RsaNI	GTAC	7 cut(s)	927, 965, 983, 1985, 2005, 2304, 3087
RseI	CAYNNNNRTG	2 cut(s)	940, 1091
SacI	GAGCTC	1 cut(s)	1795
SapI	GCTCTTC	1 cut(s)	2775
Sau96I	GGNCC	3 cut(s)	812, 2045, 2665
ScaI	AGTACT	1 cut(s)	1986
SchI	GAGTC	4 cut(s)	80, 779, 2105, 2813
ScrFI	CCNGG	7 cut(s)	134, 1892, 1965, 2001, 2262, 2637, 2663
SduI	GDGCHC	7 cut(s)	325, 877, 1614, 1795, 1941, 2846, 3044
SexAI	ACCWGGT	1 cut(s)	2260
SfaNI	GCATC	6 cut(s)	66, 1396, 2667, 2734, 2925, 2989
SfcI	CTRYAG	4 cut(s)	1209, 1605, 2541, 2610
SfoI	GGCGCC	1 cut(s)	999
SfuI	TTCGAA	2 cut(s)	141, 1504
SgrAI	CRCCGGYG	1 cut(s)	1000
SinI	GGWCC	2 cut(s)	812, 2665
SmiMI	CAYNNNNRTG	2 cut(s)	940, 1091
SmlI	CTYRAG	4 cut(s)	107, 464, 1613, 1833
SmoI	CTYRAG	4 cut(s)	107, 464, 1613, 1833
SsiI	CCGC	7 cut(s)	428, 479, 972, 1677, 1862, 2668, 2740
SspDI	GGCGCC	1 cut(s)	997
SspI	AATATT	2 cut(s)	504, 824
SspMI	CTAG	4 cut(s)	396, 968, 1296, 1826
SstI	GAGCTC	1 cut(s)	1795
StyD4I	CCNGG	7 cut(s)	132, 1890, 1963, 1999, 2260, 2635, 2661
StyI	CCWWGG	1 cut(s)	1592
TaiI	ACGT	6 cut(s)	54, 357, 1746, 1888, 2131, 2309
TaqI	TCGA	9 cut(s)	72, 84, 141, 266, 700, 1504, 2871, 2970, 3177
TaqII	GACCGA	1 cut(s)	1803
TatI	WGTACW	4 cut(s)	926, 964, 1984, 3086
TauI	GCSGC	3 cut(s)	431, 974, 2743
TscAI	CASTG	6 cut(s)	330, 961, 1614, 1932, 2044, 2857
TseFI	GTSAC	6 cut(s)	749, 1784, 2074, 2084, 2500, 2647
Tsp45I	GTSAC	6 cut(s)	749, 1784, 2074, 2084, 2500, 2647
TspGWI	ACGGA	2 cut(s)	294, 2291
TspRI	CASTG	6 cut(s)	330, 961, 1614, 1932, 2044, 2857
Tth111I	GACNNNGTC	2 cut(s)	50, 2491
VneI	GTGCAC	1 cut(s)	2842
VpaK11BI	GGWCC	2 cut(s)	812, 2665
VspI	ATTAAT	1 cut(s)	270
XapI	RAATTY	7 cut(s)	694, 863, 1241, 1336, 2060, 2900, 3166
XbaI	TCTAGA	1 cut(s)	1825
XmiI	GTMKAC	1 cut(s)	2544
XmnI	GAANNNNTTC	1 cut(s)	515
XspI	CTAG	4 cut(s)	396, 968, 1296, 1826
ZraI	GACGTC	1 cut(s)	52
ZrmI	AGTACT	1 cut(s)	1986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.