MD01G1135400.v1.1

Belongs to the adaptor complexes large subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009227
Physical Location & Seq
Reverse (-)
24456626 .. 24462394
5769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1135400.v1.1.491

Sequence Viewer

Length: 3390 bp
ATGTTCAATCAGTTCGGAGCAACGGCGGAGACTCTAAGCAAGGCGTCGACACTGGTGTTCCGGATCGGCACCGACGCCCACCTCTACGACGATCCCGACGACGTCAGCATCGCTCCTCTCCTGGACAGCAGGTTCGACTCCGAGAAGTGCGAGGCTCTCAAGCGTTTACTCGCTCTCATCGCCCAGGGATTCGAAGTCTCCAATTTCTTCCCTCAGGTTGTGAAGAACGTAGCGTCGCAGTCATTGGAAGTGAAGAAGCTCGTTTACGTGTACTTGCTGCATTATGCTCAAAAGCGTCCGAATGAAGCGTTGCTGTCAATTAATTATTTCCAGAAGGATTTAGGGGATCCAAATCCGTTAGTGAGGGCGTGGGCGCTGAGAACCATGGCGGGGATACGCCTGCACGTTATTGCACCTTTGGTTCTTGTTGCAGCCAGAAAATGTGCTAGAGATCCGTCTGTGTATGTTAGGAAATGCGCGGCCAATGCACTTCCTAAGCTGCACGATTTGCGCCTTGAGGAGAATACTGTGGGGATTGAAGAGATTATTGGAACATTGTTGAATGACTCTTCCCCTTGTGTCGTTGGAGCTGCTGCTGCTGCTTTCTCTTCTGTTTGTCCAAATAATTTGTCACTGATTGGAAGGAACTATAAAAGGTTATGTGAGGTTCTTCCTGATGTGGAAGAATGGGGTAAAATAGTTTTAATTGGGATCCTTTTGCGCTATGTAGTCGCAAGGCATGGGCTTATAAAGGAATCCATTATGTTCTCTTTGCATGGAACAGCGAATTCTCGATCTGAAAAGGATTGTGCGGACACCAACTCTGCGTTGGATGACGATGGTGACATTAATGGTCTTTATGAGTCTGAATTAACAAATGCTGTATCCCGTTGTTATATTGAAGGACCAGCTGAATACTTATCACGATTAAGTTTCATGAATAAAGATTCCTCTGAATTTAATTATGCTCGTTTTACATCTGGAAAGAATAACGATGATGTGAAGATCCTGCTGCAATGTACATCGCCATTGTTGTGGAGTAATAATAGTGCAGTTGTACTAGCAGCTGCTGGTGTACATTGGATTATGGCATCAACGGAGGATTTAAAAAGAATCATTAAGCCGCTCTTGTTCGTTCTAAGATCATCCAATGCCTCAAAATATGTGGTTTTATGTAACATTCAAGTATTTGCCAAAGCAATTCCTTCACTCTTCTCTCCATACTTTGAAGACTTCTTTATATGCTCTTCGGAATCATATCAAATTAAAGCTTTGAAACTGGATATACTGGCTTACATCGCTACAGATTCATCAATTTCGTTTATACTCAAAGAATTTCAGGATTATATTAGAGATCCCGACAGGAGATTTGCTGCCGATACTGTTGCTGGAATTGGCATATGTGCGCAACGACTTCCAGAAATGGCAAATGCATGCTTGGAATTTCTATTGGCTTTGACCAGACAGCAACTTATGACTGGTGAGTTTGGGTCTGTGGATGCCGAAGCAGATATATTGATTCAAGCAATAATGTCTATTAAGTCAATCATTCAGCTAGATCCACCGAGTCATGAAAAGGTTATTATTCAATTGGTTCGAAGTTTAACTTCAATAAGGGTGCCTGCTGCACGTGCTATGGTTGTTTGGATGCTGGGGGAGTATAACTCTTTAGGTGATCTCATCCCAAGGATGTTAGCTACAGTGCTCAAGTATCTTGCAGGGTGCTTTGCTTCTGAAGAAGTGGAAACAAAGCTTCAGATTTGTAATACCGCTGTTAAGGTTTTATTGGGGGCAAAAGGGGATGACGTGTTGACAATCAAAATGGTTTTAATCTACGTGTTGGAGCTGGCCAAATGCGACTTAAATTATGACGTTCGTGACCGAGCTCATTTCTTAAAGAAACTCCTGTCAACTTATTTAGATTCTCAATGTCTGGAGGAGGGAACCAACCGTCCGGGTCAACAAAAAGATAGTTCACTTCTCCTGGCAGAAAACTTGTTTGGGAAACAAAATAAACCAGTGTGCCATGAGCCCATTGATCACCGGTTTTACCTTCCTGGCTCTTTATCGCAGATAGTACTTCATGCTGCTCCAGGGTATGAGCCTCTCCCAAAGCCCTGTAGTTTGCGCTGTGATGGCCCCGAAATGAATGAAAATGGTGAATCATATGTTACAGACGATGAGGACTCAGCGTCTGAATCTTTGGATGAGGAAATTGCTTCTAGTTACAGTTCGCAACGTTCTAATGTTGATTCAACTGGCACTGATGACGGTGAAGACGCTGGTTCTGCAAGTGAAGACGATGACAATTCTCATCAGTTGATTCAGTTTTCAGATGAAAGGAAAAATGGAGCTTCTCAGTCTGCTTCTGATTTTGGCGAATTGTTGTCAAATAGAGCTTTAGAATCATGGTTAGATGACCAACCTGGTTTTTCAAAACCAAATACTTCAGAACACACTCAACACAGTCAAGTCCGTACATCTTCAGCAAGAATCTCCATCGGGGATATTGGAGGTCAAGTTAGACCTAAAAGATATCCACTCTTGGACCCTGTGAATGGAAATGGCTTAAAGGTTGACTATTCATTTTCATCTGAGATTTCAAGTATCTCTCCTCTGTTTATATGTATAGAAGTTTCCTTCAAGAACTGCTCAAATGAGACCATGTCTGATATAACTTTTGTGGATGAGGAATCCGGAAAAGGCAAGGACTCTGTAGAGCAATCGTCGTTTTCAGATGAAAGCTCCACGATACCTCAAAGTAATGAGCCAAATCTAGTTGCTGTAGAAGAGATCACTTCTCTGGAACCTGGTCAGACTATAACAAGAAGCATCCAGGTCCGCTTCCATCACCACCTCTTGCCTCTAAAACTGGCCTTATATTGTAACGGCAAGAGGCATCCTATTAAGTTGCGGCCTGACATTGGATACTTTGTAAAAGCTCTTCCTATGGATGTTGAAGCCTTTACAAATAAGGAGTCTCAACTGCGTGGAATGTTTGAATGCGTAAGAAGGTGCACCTTCACTGACCATATCAAGGAGTTGGACAAGGACAAGGGTGATAATTCATTGGTGGAAGACAAATTTCTCGTAATCTGCCGAAGTCTTGCATTGAAGATGCTCAGCAATGCAAATCTTCATCTTGTATCCGTTGATTTGCCAGTTGCAGCAAAGCTTGATGATGCAACAGGCCTGTGCTTACGGTTCAGCAGCAAGATTTTAAGCACCTCTGCCCCCTGCTTGATAACCATTACTGTCGAAGGTAGATGTTCCGAACCATTGGAAATGTCAGTTAAAGTAAACTGTGAAGAAACTGTGTTTGGGTTGAATCTTCTGAACAGGATCGTAAATTTCTTAGTTGAGCCCTCCCATGCCCACGTGAGTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
AP3B1_C Adaptin_N

Protein Analysis

1130

Amino Acids

124.78

Weight (kDa)

5.17

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 40 - 638 1.6e-94 Adaptin N terminal region
Cnd1 PF12717 120 - 247 8.7e-13 non-SMC mitotic condensation complex subunit 1
AP3B1_C PF14796 811 - 900 1.7e-08 Clathrin-adaptor complex-3 beta-1 subunit C-terminal
AP3B1_C_2 PF24080 978 - 1118 1.2e-07 AP-3 complex subunit beta-1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 749
AatII GACGTC 1 cut(s) 105
Acc16I TGCGCA 1 cut(s) 1407
Acc36I ACCTGC 1 cut(s) 120
AccB1I GGYRCC 2 cut(s) 68, 1618
AccBSI CCGCTC 1 cut(s) 1126
AccI GTMKAC 1 cut(s) 47
AccII CGCG 1 cut(s) 479
AccIII TCCGGA 2 cut(s) 60, 2696
AciI CCGC 8 cut(s) 26, 389, 479, 812, 1124, 1770, 2842, 2914
AclI AACGTT 1 cut(s) 2239
AcoI YGGCCR 2 cut(s) 480, 1848
AcsI RAATTY 6 cut(s) 787, 956, 1334, 1442, 3083, 3349
AcuI CTGAAG 4 cut(s) 1739, 1755, 2433, 2469
AcvI CACGTG 2 cut(s) 1631, 3379
AcyI GRCGYC 3 cut(s) 44, 75, 102
AfaI GTAC 6 cut(s) 272, 1021, 1059, 1077, 2079, 2479
AfiI CCNNNNNNNGG 4 cut(s) 390, 2558, 2924, 3037
AflIII ACRYGT 3 cut(s) 267, 1806, 1836
AgeI ACCGGT 1 cut(s) 2043
AhdI GACNNNNNGTC 1 cut(s) 2191
AjiI CACGTC 1 cut(s) 1807
AjnI CCWGG 8 cut(s) 120, 183, 1983, 2056, 2092, 2425, 2809, 2835
AjuI GAANNNNNNNTTGG 6 cut(s) 531, 563, 1983, 2015, 3303, 3335
AloI GAACNNNNNNTCC 4 cut(s) 116, 148, 1936, 1968
Alw21I GWGCWC 3 cut(s) 1707, 1888, 3020
Alw26I GTCTC 4 cut(s) 23, 202, 2654, 2985
Alw44I GTGCAC 1 cut(s) 3016
AlwNI CAGNNNCTG 2 cut(s) 1070, 2195
Aor13HI TCCGGA 2 cut(s) 60, 2696
AoxI GGCC 6 cut(s) 480, 1848, 2137, 2874, 2915, 3190
ApaLI GTGCAC 1 cut(s) 3016
ApoI RAATTY 6 cut(s) 787, 956, 1334, 1442, 3083, 3349
ArsI GACNNNNNNTTYG 8 cut(s) 116, 148, 614, 646, 1352, 1384, 2963, 2995
AseI ATTAAT 2 cut(s) 321, 849
AsiGI ACCGGT 1 cut(s) 2043
AspLEI GCGC 6 cut(s) 376, 479, 513, 723, 1408, 2130
AspS9I GGNCC 4 cut(s) 905, 2138, 2548, 2839
AsuC2I CCSGG 1 cut(s) 1956
AsuHPI GGTGA 8 cut(s) 854, 1493, 1685, 2033, 2171, 2285, 2843, 3071
AsuII TTCGAA 2 cut(s) 192, 1597
AvaII GGWCC 3 cut(s) 905, 2548, 2839
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 3020
BalI TGGCCA 1 cut(s) 1850
BamHI GGATCC 2 cut(s) 346, 711
BanI GGYRCC 2 cut(s) 68, 1618
BanII GRGCYC 3 cut(s) 1888, 2034, 3366
BarI GAAGNNNNNNTAC 4 cut(s) 248, 280, 2928, 2960
BbrPI CACGTG 2 cut(s) 1631, 3379
BbsI GAAGAC 4 cut(s) 1236, 2283, 2304, 3084
Bbv12I GWGCWC 3 cut(s) 1707, 1888, 3020
BccI CCATC 4 cut(s) 833, 2129, 2507, 2856
BceAI ACGGC 2 cut(s) 39, 2905
BcgI CGANNNNNNTGC 2 cut(s) 1367, 1401
BciT130I CCWGG 8 cut(s) 122, 185, 1985, 2058, 2094, 2427, 2811, 2837
BciVI GTATCC 4 cut(s) 387, 895, 2921, 3157
BclI TGATCA 1 cut(s) 2038
BcnI CCSGG 1 cut(s) 1956
BcoDI GTCTC 4 cut(s) 23, 202, 2654, 2985
BfaI CTAG 5 cut(s) 447, 1061, 1556, 2223, 2777
BfmI CTRYAG 5 cut(s) 1302, 1698, 2119, 2715, 2784
BfoI RGCGCY 1 cut(s) 377
BfuAI ACCTGC 1 cut(s) 120
BfuI GTATCC 4 cut(s) 387, 895, 2921, 3157
BlpI GCTNAGC 1 cut(s) 3122
BmcAI AGTACT 1 cut(s) 2079
Bme1390I CCNGG 9 cut(s) 122, 185, 1956, 1985, 2058, 2094, 2427, 2811, 2837
Bme18I GGWCC 3 cut(s) 905, 2548, 2839
BmeRI GACNNNNNGTC 1 cut(s) 2191
BmgBI CACGTC 1 cut(s) 1807
BmgT120I GGNCC 4 cut(s) 905, 2138, 2548, 2839
BmiI GGNNCC 8 cut(s) 70, 348, 713, 1620, 1945, 2140, 2550, 2808
BmrFI CCNGG 9 cut(s) 122, 185, 1956, 1985, 2058, 2094, 2427, 2811, 2837
BmsI GCATC 8 cut(s) 117, 1100, 1489, 1638, 2841, 2908, 3108, 3172
BpiI GAAGAC 4 cut(s) 1236, 2283, 2304, 3084
BplI GAGNNNNNCTC 2 cut(s) 1649, 1681
BpmI CTGGAG 2 cut(s) 1955, 2076
Bpu10I CCTNAGC 1 cut(s) 495
Bpu1102I GCTNAGC 1 cut(s) 3122
Bpu14I TTCGAA 2 cut(s) 192, 1597
BpuEI CTTGAG 3 cut(s) 143, 536, 1691
BpuMI CCSGG 1 cut(s) 1956
BsaAI YACGTR 4 cut(s) 268, 1631, 1837, 3379
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 3 cut(s) 44, 75, 102
BsaI GGTCTC 1 cut(s) 2654
BsaJI CCNNGG 5 cut(s) 183, 184, 384, 1685, 2093
BsaWI WCCGGW 3 cut(s) 60, 2043, 2696
BsaXI ACNNNNNCTCC 2 cut(s) 2505, 2535
Bsc4I CCNNNNNNNGG 4 cut(s) 390, 2558, 2924, 3037
Bse118I RCCGGY 1 cut(s) 2043
Bse1I ACTGG 8 cut(s) 57, 1284, 1293, 1483, 2018, 2263, 2877, 3161
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 2 cut(s) 1022, 3133
Bse8I GATNNNNATC 1 cut(s) 351
BseAI TCCGGA 2 cut(s) 60, 2696
BseBI CCWGG 8 cut(s) 122, 185, 1985, 2058, 2094, 2427, 2811, 2837
BseDI CCNNGG 5 cut(s) 183, 184, 384, 1685, 2093
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 4 cut(s) 390, 2558, 2924, 3037
BseMI GCAATG 2 cut(s) 1022, 3133
BseMII CTCAG 6 cut(s) 227, 368, 2202, 2372, 2586, 3136
BseNI ACTGG 8 cut(s) 57, 1284, 1293, 1483, 2018, 2263, 2877, 3161
BseRI GAGGAG 4 cut(s) 105, 533, 1952, 2604
BseSI GKGCMC 1 cut(s) 3020
BseYI CCCAGC 1 cut(s) 1651
BsgI GTGCAG 4 cut(s) 386, 485, 1071, 1611
Bsh1236I CGCG 1 cut(s) 479
BshFI GGCC 6 cut(s) 482, 1850, 2139, 2876, 2917, 3192
BshNI GGYRCC 2 cut(s) 68, 1618
BshTI ACCGGT 1 cut(s) 2043
BsiHKAI GWGCWC 3 cut(s) 1707, 1888, 3020
BsiSI CCGG 4 cut(s) 61, 1955, 2044, 2697
BslI CCNNNNNNNGG 4 cut(s) 390, 2558, 2924, 3037
BsmAI GTCTC 4 cut(s) 23, 202, 2654, 2985
BsmI GAATGC 1 cut(s) 3008
BsnI GGCC 6 cut(s) 482, 1850, 2139, 2876, 2917, 3192
Bso31I GGTCTC 1 cut(s) 2654
Bsp119I TTCGAA 2 cut(s) 192, 1597
Bsp1286I GDGCHC 5 cut(s) 1707, 1888, 2034, 3020, 3366
Bsp13I TCCGGA 2 cut(s) 60, 2696
Bsp1407I TGTACA 2 cut(s) 1019, 1075
Bsp1720I GCTNAGC 1 cut(s) 3122
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 8 cut(s) 26, 389, 479, 812, 1124, 1770, 2842, 2914
BspANI GGCC 6 cut(s) 482, 1850, 2139, 2876, 2917, 3192
BspCNI CTCAG 6 cut(s) 226, 369, 2201, 2371, 2587, 3135
BspEI TCCGGA 2 cut(s) 60, 2696
BspFNI CGCG 1 cut(s) 479
BspHI TCATGA 2 cut(s) 936, 1570
BspLI GGNNCC 8 cut(s) 70, 348, 713, 1620, 1945, 2140, 2550, 2808
BspMI ACCTGC 1 cut(s) 120
BspQI GCTCTTC 2 cut(s) 1252, 2949
BspT104I TTCGAA 2 cut(s) 192, 1597
BspT107I GGYRCC 2 cut(s) 68, 1618
BspTNI GGTCTC 1 cut(s) 2654
BsrBI CCGCTC 1 cut(s) 1126
BsrDI GCAATG 2 cut(s) 1022, 3133
BsrFI RCCGGY 1 cut(s) 2043
BsrGI TGTACA 2 cut(s) 1019, 1075
BsrI ACTGG 8 cut(s) 57, 1284, 1293, 1483, 2018, 2263, 2877, 3161
BssAI RCCGGY 1 cut(s) 2043
BssECI CCNNGG 5 cut(s) 183, 184, 384, 1685, 2093
BssNI GRCGYC 3 cut(s) 44, 75, 102
BssT1I CCWWGG 2 cut(s) 384, 1685
Bst2UI CCWGG 8 cut(s) 122, 185, 1985, 2058, 2094, 2427, 2811, 2837
Bst6I CTCTTC 7 cut(s) 534, 574, 613, 1217, 1252, 2784, 2949
BstACI GRCGYC 3 cut(s) 44, 75, 102
BstAPI GCANNNNNTGC 1 cut(s) 508
BstAUI TGTACA 2 cut(s) 1019, 1075
BstBAI YACGTR 4 cut(s) 268, 1631, 1837, 3379
BstBI TTCGAA 2 cut(s) 192, 1597
BstC8I GCNNGC 4 cut(s) 401, 1435, 1623, 1848
BstDSI CCRYGG 1 cut(s) 384
BstFNI CGCG 1 cut(s) 479
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 6 cut(s) 376, 479, 513, 723, 1408, 2130
BstMAI GTCTC 4 cut(s) 23, 202, 2654, 2985
BstMWI GCNNNNNNNGC 9 cut(s) 179, 485, 508, 596, 599, 1298, 1631, 2136, 2288
BstNI CCWGG 8 cut(s) 122, 185, 1985, 2058, 2094, 2427, 2811, 2837
BstNSI RCATGY 1 cut(s) 1437
BstSCI CCNGG 9 cut(s) 120, 183, 1954, 1983, 2056, 2092, 2425, 2809, 2835
BstSFI CTRYAG 5 cut(s) 1302, 1698, 2119, 2715, 2784
BstSLI GKGCMC 1 cut(s) 3020
BstUI CGCG 1 cut(s) 479
BstV2I GAAGAC 4 cut(s) 1236, 2283, 2304, 3084
BstX2I RGATCY 6 cut(s) 346, 451, 711, 1005, 1354, 1558
BstXI CCANNNNNNTGG 1 cut(s) 1035
BstYI RGATCY 6 cut(s) 346, 451, 711, 1005, 1354, 1558
Bsu36I CCTNAGG 1 cut(s) 213
BsuI GTATCC 4 cut(s) 387, 895, 2921, 3157
BsuRI GGCC 6 cut(s) 482, 1850, 2139, 2876, 2917, 3192
BtgI CCRYGG 1 cut(s) 384
BtgZI GCGATG 4 cut(s) 94, 163, 1008, 1282
BtrI CACGTC 1 cut(s) 1807
BtsIMutI CAGTG 6 cut(s) 50, 632, 1707, 2025, 2262, 3024
BveI ACCTGC 1 cut(s) 120
Cac8I GCNNGC 4 cut(s) 401, 1435, 1623, 1848
CaiI CAGNNNCTG 2 cut(s) 1070, 2195
CciI TCATGA 2 cut(s) 936, 1570
CfoI GCGC 6 cut(s) 376, 479, 513, 723, 1408, 2130
Cfr10I RCCGGY 1 cut(s) 2043
Cfr13I GGNCC 4 cut(s) 905, 2138, 2548, 2839
CseI GACGC 6 cut(s) 33, 83, 222, 284, 2181, 2288
CsiI ACCWGGT 2 cut(s) 2425, 2809
Csp6I GTAC 6 cut(s) 271, 1020, 1058, 1076, 2078, 2478
CspAI ACCGGT 1 cut(s) 2043
CspCI CAANNNNNGTGG 2 cut(s) 1146, 1181
CviQI GTAC 6 cut(s) 271, 1020, 1058, 1076, 2078, 2478
DraI TTTAAA 1 cut(s) 1107
DriI GACNNNNNGTC 1 cut(s) 2191
EaeI YGGCCR 2 cut(s) 480, 1848
Eam1104I CTCTTC 7 cut(s) 534, 574, 613, 1217, 1252, 2784, 2949
Eam1105I GACNNNNNGTC 1 cut(s) 2191
EarI CTCTTC 7 cut(s) 534, 574, 613, 1217, 1252, 2784, 2949
EciI GGCGGA 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 1886
Eco130I CCWWGG 2 cut(s) 384, 1685
Eco147I AGGCCT 1 cut(s) 3192
Eco24I GRGCYC 3 cut(s) 1888, 2034, 3366
Eco31I GGTCTC 1 cut(s) 2654
Eco32I GATATC 1 cut(s) 2537
Eco47I GGWCC 3 cut(s) 905, 2548, 2839
Eco53kI GAGCTC 1 cut(s) 1886
Eco57I CTGAAG 4 cut(s) 1739, 1755, 2433, 2469
Eco72I CACGTG 2 cut(s) 1631, 3379
Eco81I CCTNAGG 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 1886
EcoRI GAATTC 1 cut(s) 787
EcoRII CCWGG 8 cut(s) 120, 183, 1983, 2056, 2092, 2425, 2809, 2835
EcoRV GATATC 1 cut(s) 2537
EcoT14I CCWWGG 2 cut(s) 384, 1685
EcoT22I ATGCAT 1 cut(s) 1435
EcoT38I GRGCYC 3 cut(s) 1888, 2034, 3366
ErhI CCWWGG 2 cut(s) 384, 1685
FalI AAGNNNNNCTT 4 cut(s) 1112, 1144, 1591, 1623
FauI CCCGC 1 cut(s) 382
FauNDI CATATG 2 cut(s) 1400, 2167
FbaI TGATCA 1 cut(s) 2038
FblI GTMKAC 1 cut(s) 47
FriOI GRGCYC 3 cut(s) 1888, 2034, 3366
FspBI CTAG 5 cut(s) 447, 1061, 1556, 2223, 2777
FspI TGCGCA 1 cut(s) 1407
GlaI GCGC 6 cut(s) 375, 478, 512, 722, 1407, 2129
GsaI CCCAGC 1 cut(s) 1655
GsuI CTGGAG 2 cut(s) 1955, 2076
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 6 cut(s) 482, 1850, 2139, 2876, 2917, 3192
HapII CCGG 4 cut(s) 61, 1955, 2044, 2697
HgaI GACGC 6 cut(s) 33, 83, 222, 284, 2181, 2288
HhaI GCGC 6 cut(s) 376, 479, 513, 723, 1408, 2130
Hin1I GRCGYC 3 cut(s) 44, 75, 102
Hin6I GCGC 6 cut(s) 374, 477, 511, 721, 1406, 2128
HinP1I GCGC 6 cut(s) 374, 477, 511, 721, 1406, 2128
HincII GTYRAC 5 cut(s) 48, 1812, 1911, 1961, 2578
HindII GTYRAC 5 cut(s) 48, 1812, 1911, 1961, 2578
HindIII AAGCTT 3 cut(s) 1269, 1751, 3173
HpaII CCGG 4 cut(s) 61, 1955, 2044, 2697
HphI GGTGA 8 cut(s) 854, 1493, 1685, 2033, 2171, 2285, 2843, 3071
Hpy99I CGWCG 7 cut(s) 49, 77, 92, 101, 104, 238, 2731
HpyAV CCTTC 9 cut(s) 328, 636, 896, 1215, 2063, 2650, 3006, 3031, 3254
HpyF10VI GCNNNNNNNGC 9 cut(s) 179, 485, 508, 596, 599, 1298, 1631, 2136, 2288
Hsp92I GRCGYC 3 cut(s) 44, 75, 102
HspAI GCGC 6 cut(s) 374, 477, 511, 721, 1406, 2128
Kpn2I TCCGGA 2 cut(s) 60, 2696
Ksp22I TGATCA 1 cut(s) 2038
LguI GCTCTTC 2 cut(s) 1252, 2949
LmnI GCTCC 7 cut(s) 17, 118, 587, 1843, 2095, 2351, 2750
LweI GCATC 8 cut(s) 117, 1100, 1489, 1638, 2841, 2908, 3108, 3172
MabI ACCWGGT 2 cut(s) 2425, 2809
MaeI CTAG 5 cut(s) 447, 1061, 1556, 2223, 2777
MaeIII GTNAC 7 cut(s) 630, 842, 1175, 1877, 2170, 2225, 2885
MbiI CCGCTC 1 cut(s) 1126
MfeI CAATTG 1 cut(s) 1589
MflI RGATCY 6 cut(s) 346, 451, 711, 1005, 1354, 1558
MhlI GDGCHC 5 cut(s) 1707, 1888, 2034, 3020, 3366
MlsI TGGCCA 1 cut(s) 1850
MluNI TGGCCA 1 cut(s) 1850
MlyI GAGTC 8 cut(s) 25, 131, 560, 872, 1576, 2180, 2705, 2987
MmeI TCCRAC 4 cut(s) 565, 810, 1821, 3024
Mox20I TGGCCA 1 cut(s) 1850
Mph1103I ATGCAT 1 cut(s) 1435
MroI TCCGGA 2 cut(s) 60, 2696
MscI TGGCCA 1 cut(s) 1850
MslI CAYNNNNRTG 1 cut(s) 1033
Msp20I TGGCCA 1 cut(s) 1850
MspA1I CMGCKG 3 cut(s) 911, 1067, 1772
MspI CCGG 4 cut(s) 61, 1955, 2044, 2697
MspR9I CCNGG 9 cut(s) 122, 185, 1956, 1985, 2058, 2094, 2427, 2811, 2837
MunI CAATTG 1 cut(s) 1589
Mva1269I GAATGC 1 cut(s) 3008
MvaI CCWGG 8 cut(s) 122, 185, 1985, 2058, 2094, 2427, 2811, 2837
MvnI CGCG 1 cut(s) 479
MwoI GCNNNNNNNGC 9 cut(s) 179, 485, 508, 596, 599, 1298, 1631, 2136, 2288
NciI CCSGG 1 cut(s) 1956
NcoI CCATGG 1 cut(s) 384
NdeI CATATG 2 cut(s) 1400, 2167
NlaIV GGNNCC 8 cut(s) 70, 348, 713, 1620, 1945, 2140, 2550, 2808
NmuCI GTSAC 3 cut(s) 630, 842, 1877
NsbI TGCGCA 1 cut(s) 1407
NsiI ATGCAT 1 cut(s) 1435
NspI RCATGY 1 cut(s) 1437
NspV TTCGAA 2 cut(s) 192, 1597
PaeI GCATGC 1 cut(s) 1437
PagI TCATGA 2 cut(s) 936, 1570
PasI CCCWGGG 1 cut(s) 184
PceI AGGCCT 1 cut(s) 3192
PciSI GCTCTTC 2 cut(s) 1252, 2949
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 96
PctI GAATGC 1 cut(s) 3008
PflFI GACNNNGTC 2 cut(s) 101, 2665
PfoI TCCNGGA 1 cut(s) 120
PinAI ACCGGT 1 cut(s) 2043
PleI GAGTC 8 cut(s) 25, 131, 560, 871, 1575, 2180, 2705, 2986
PmaCI CACGTG 2 cut(s) 1631, 3379
PmlI CACGTG 2 cut(s) 1631, 3379
PpsI GAGTC 8 cut(s) 25, 131, 560, 871, 1575, 2180, 2705, 2986
Ppu21I YACGTR 4 cut(s) 268, 1631, 1837, 3379
PshBI ATTAAT 2 cut(s) 321, 849
PsiI TTATAA 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 1888
Psp1406I AACGTT 1 cut(s) 2239
Psp6I CCWGG 8 cut(s) 120, 183, 1983, 2056, 2092, 2425, 2809, 2835
PspCI CACGTG 2 cut(s) 1631, 3379
PspFI CCCAGC 1 cut(s) 1651
PspGI CCWGG 8 cut(s) 120, 183, 1983, 2056, 2092, 2425, 2809, 2835
PspN4I GGNNCC 8 cut(s) 70, 348, 713, 1620, 1945, 2140, 2550, 2808
PspPI GGNCC 4 cut(s) 905, 2138, 2548, 2839
PstNI CAGNNNCTG 2 cut(s) 1070, 2195
PsuI RGATCY 6 cut(s) 346, 451, 711, 1005, 1354, 1558
PsyI GACNNNGTC 2 cut(s) 101, 2665
PvuII CAGCTG 2 cut(s) 911, 1067
RsaI GTAC 6 cut(s) 272, 1021, 1059, 1077, 2079, 2479
RsaNI GTAC 6 cut(s) 271, 1020, 1058, 1076, 2078, 2478
RseI CAYNNNNRTG 1 cut(s) 1033
SacI GAGCTC 1 cut(s) 1888
SalI GTCGAC 1 cut(s) 46
SapI GCTCTTC 2 cut(s) 1252, 2949
Sau96I GGNCC 4 cut(s) 905, 2138, 2548, 2839
ScaI AGTACT 1 cut(s) 2079
SchI GAGTC 8 cut(s) 25, 131, 560, 872, 1576, 2180, 2705, 2987
ScrFI CCNGG 9 cut(s) 122, 185, 1956, 1985, 2058, 2094, 2427, 2811, 2837
SduI GDGCHC 5 cut(s) 1707, 1888, 2034, 3020, 3366
SexAI ACCWGGT 2 cut(s) 2425, 2809
SfaNI GCATC 8 cut(s) 117, 1100, 1489, 1638, 2841, 2908, 3108, 3172
SfcI CTRYAG 5 cut(s) 1302, 1698, 2119, 2715, 2784
SfuI TTCGAA 2 cut(s) 192, 1597
SinI GGWCC 3 cut(s) 905, 2548, 2839
SmiMI CAYNNNNRTG 1 cut(s) 1033
SmlI CTYRAG 3 cut(s) 158, 515, 1706
SmoI CTYRAG 3 cut(s) 158, 515, 1706
SphI GCATGC 1 cut(s) 1437
SseBI AGGCCT 1 cut(s) 3192
SsiI CCGC 8 cut(s) 26, 389, 479, 812, 1124, 1770, 2842, 2914
SspMI CTAG 5 cut(s) 447, 1061, 1556, 2223, 2777
SstI GAGCTC 1 cut(s) 1888
StuI AGGCCT 1 cut(s) 3192
StyD4I CCNGG 9 cut(s) 120, 183, 1954, 1983, 2056, 2092, 2425, 2809, 2835
StyI CCWWGG 2 cut(s) 384, 1685
TaqI TCGA 6 cut(s) 47, 135, 192, 793, 1597, 3258
TaqII GACCGA 1 cut(s) 1896
TatI WGTACW 5 cut(s) 270, 1019, 1057, 1075, 2077
TauI GCSGC 3 cut(s) 482, 1126, 2917
TscAI CASTG 6 cut(s) 57, 639, 1707, 2025, 2269, 3031
TseFI GTSAC 3 cut(s) 630, 842, 1877
Tsp45I GTSAC 3 cut(s) 630, 842, 1877
TspGWI ACGGA 5 cut(s) 345, 444, 1112, 2465, 3139
TspRI CASTG 6 cut(s) 57, 639, 1707, 2025, 2269, 3031
Tth111I GACNNNGTC 2 cut(s) 101, 2665
VneI GTGCAC 1 cut(s) 3016
VpaK11BI GGWCC 3 cut(s) 905, 2548, 2839
VspI ATTAAT 2 cut(s) 321, 849
XapI RAATTY 6 cut(s) 787, 956, 1334, 1442, 3083, 3349
XceI RCATGY 1 cut(s) 1437
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XmiI GTMKAC 1 cut(s) 47
XspI CTAG 5 cut(s) 447, 1061, 1556, 2223, 2777
ZraI GACGTC 1 cut(s) 103
ZrmI AGTACT 1 cut(s) 2079
Zsp2I ATGCAT 1 cut(s) 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.