FvH4_7g28400

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014997
Physical Location & Seq
Forward (+)
21047692 .. 21049101
1410 bp
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UTR
Exon/CDS
Intron
FvH4_7g28400.t1

Sequence Viewer

Length: 552 bp
ATGATCAAAGCGCTCTCTTTTCTTCTGCTCCTAGTCGCCTCCGTCTCCACGCCTTCTGAAGCCAAATTGAGCATCCTCCGACCCCAACCCGCCGTAGGTTTCAACGTCAGCCATGTACAGAGTACCGGGACTTGTTCTTATACTGTGGACATAACCACGAGCTGTTCGTCAACGAGGTACACAAGGGATCAAATCAGTATTTCATTCGGAGATGTTTATGGCAACCAGATATATGCACCGCGGCTAGATGATCCATCAGCGAAAACGTTTGAGAGTTGTTCTACAGATACATTTGAGATATATGGACCATGCGCATATCAAATCTGTTATGTGTATCTTTACAGAACAGGACCTGATGGTTGGAAACCGGAGAGTGTGAAAATCTATAGTCACAATTCCAGGGCTGTTTCGTTCTACTACAACACCTTCATTCCCAGTGACATTTGGTATGGATTCAATTTGTGCCAGAATGGTTCATCTTCACACCGGCAATGTGCTTGTGGATGGTTTATATATGTTGTAGTTGGGCTTCTTGTGTCGCTTCTCTTGTAG

Protein Analysis

184

Amino Acids

20.38

Weight (kDa)

6.79

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 40 - 155 6e-53 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62200
fragaria_vesca FvH4_7g28400
malus_domestica MD01G1187700.v1.1 MD07G1259800.v1.1
prunus_persica Prupe.2G284100_v2.0.a1
pyrus_communis pycom01g19960 pycom07g23320
rosa_chinensis RchiOBHm_Chr1g0375361
rosa_laevigata RLG00000026668
rosa_rugosa Rorug01G0390500.1 Rorug01G0390600
rosa_samantha Rh1AG401000 Rh1BG363300 Rh1CG376600 Rh1DG394100
rosa_wichuraiana Rw1G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 313
AccII CGCG 1 cut(s) 241
AciI CCGC 3 cut(s) 90, 239, 241
AclI AACGTT 1 cut(s) 266
AclWI GGATC 2 cut(s) 195, 245
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 3 cut(s) 117, 124, 179
AfeI AGCGCT 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 2 cut(s) 103, 457
AjnI CCWGG 1 cut(s) 398
AluBI AGCT 1 cut(s) 162
AluI AGCT 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 2 cut(s) 195, 245
AlwNI CAGNNNCTG 1 cut(s) 353
Aor51HI AGCGCT 1 cut(s) 12
AspLEI GCGC 2 cut(s) 13, 314
AspS9I GGNCC 2 cut(s) 305, 350
AsuC2I CCSGG 1 cut(s) 127
AvaII GGWCC 2 cut(s) 305, 350
BarI GAAGNNNNNNTAC 2 cut(s) 513, 545
BauI CACGAG 1 cut(s) 157
BccI CCATC 3 cut(s) 262, 350, 498
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 400
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 2 cut(s) 32, 245
BfmI CTRYAG 2 cut(s) 282, 385
BfoI RGCGCY 1 cut(s) 14
BisI GCNGC 1 cut(s) 242
BlsI GCNGC 1 cut(s) 243
Bme1390I CCNGG 2 cut(s) 127, 400
Bme18I GGWCC 2 cut(s) 305, 350
BmgT120I GGNCC 2 cut(s) 305, 350
BmrFI CCNGG 2 cut(s) 127, 400
BmrI ACTGGG 1 cut(s) 429
BmsI GCATC 1 cut(s) 81
BmuI ACTGGG 1 cut(s) 429
BpuMI CCSGG 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 239, 399
BsaWI WCCGGW 1 cut(s) 367
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse118I RCCGGY 1 cut(s) 486
Bse1I ACTGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 497
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 2 cut(s) 239, 399
BseGI GGATG 2 cut(s) 72, 509
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMI GCAATG 1 cut(s) 497
BseNI ACTGG 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 241
BsiSI CCGG 3 cut(s) 126, 368, 487
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 49
BsmBI CGTCTC 1 cut(s) 49
BsmFI GGGAC 1 cut(s) 142
Bsp1407I TGTACA 1 cut(s) 115
Bsp143I GATC 3 cut(s) 3, 187, 250
BspACI CCGC 3 cut(s) 90, 239, 241
BspFNI CGCG 1 cut(s) 241
BspPI GGATC 2 cut(s) 195, 245
BsrDI GCAATG 1 cut(s) 497
BsrFI RCCGGY 1 cut(s) 486
BsrGI TGTACA 1 cut(s) 115
BsrI ACTGG 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 486
BssECI CCNNGG 2 cut(s) 239, 399
BssMI GATC 3 cut(s) 3, 187, 250
BssSI CACGAG 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 1 cut(s) 145
BstAUI TGTACA 1 cut(s) 115
BstDSI CCRYGG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 72, 509
BstFNI CGCG 1 cut(s) 241
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 2 cut(s) 13, 314
BstKTI GATC 3 cut(s) 6, 190, 253
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 3 cut(s) 3, 187, 250
BstNI CCWGG 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 125, 398
BstSFI CTRYAG 2 cut(s) 282, 385
BstUI CGCG 1 cut(s) 241
BtgI CCRYGG 1 cut(s) 239
BtsCI GGATG 2 cut(s) 72, 509
BtsIMutI CAGTG 1 cut(s) 442
CaiI CAGNNNCTG 1 cut(s) 353
CfoI GCGC 2 cut(s) 13, 314
Cfr10I RCCGGY 1 cut(s) 486
Cfr13I GGNCC 2 cut(s) 305, 350
Cfr42I CCGCGG 1 cut(s) 242
Csp6I GTAC 3 cut(s) 116, 123, 178
CviAII CATG 2 cut(s) 113, 309
CviJI RGCY 6 cut(s) 62, 111, 162, 244, 404, 529
CviKI_1 RGCY 6 cut(s) 62, 111, 162, 244, 404, 529
CviQI GTAC 3 cut(s) 116, 123, 178
DpnI GATC 3 cut(s) 5, 189, 252
DpnII GATC 3 cut(s) 3, 187, 250
Eco47I GGWCC 2 cut(s) 305, 350
Eco47III AGCGCT 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 398
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 2 cut(s) 116, 312
FaqI GGGAC 1 cut(s) 142
FatI CATG 2 cut(s) 112, 308
FauI CCCGC 1 cut(s) 97
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 242
FokI GGATG 2 cut(s) 59, 516
Fsp4HI GCNGC 1 cut(s) 242
FspAI RTGCGCAY 1 cut(s) 313
FspBI CTAG 2 cut(s) 32, 245
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 2 cut(s) 12, 313
GluI GCNGC 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 14
HapII CCGG 3 cut(s) 126, 368, 487
HhaI GCGC 2 cut(s) 13, 314
Hin1II CATG 2 cut(s) 116, 312
Hin6I GCGC 2 cut(s) 11, 312
HinP1I GCGC 2 cut(s) 11, 312
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 1 cut(s) 453
HpaII CCGG 3 cut(s) 126, 368, 487
Hpy166II GTNNAC 3 cut(s) 148, 171, 180
Hpy188I TCNGA 3 cut(s) 58, 80, 209
Hpy8I GTNNAC 3 cut(s) 148, 171, 180
HpyAV CCTTC 2 cut(s) 63, 436
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 2 cut(s) 105, 266
HpyCH4V TGCA 1 cut(s) 236
HpySE526I ACGT 2 cut(s) 105, 266
Hsp92II CATG 2 cut(s) 116, 312
HspAI GCGC 2 cut(s) 11, 312
Ksp22I TGATCA 1 cut(s) 3
KspI CCGCGG 1 cut(s) 242
Kzo9I GATC 3 cut(s) 3, 187, 250
LmnI GCTCC 1 cut(s) 33
LweI GCATC 1 cut(s) 81
MaeI CTAG 2 cut(s) 32, 245
MaeII ACGT 2 cut(s) 105, 266
MaeIII GTNAC 2 cut(s) 389, 437
MalI GATC 3 cut(s) 5, 189, 252
MboI GATC 3 cut(s) 3, 187, 250
MboII GAAGA 2 cut(s) 14, 471
MluCI AATT 3 cut(s) 65, 394, 457
MmeI TCCRAC 2 cut(s) 103, 341
MnlI CCTC 3 cut(s) 49, 86, 168
MspA1I CMGCKG 1 cut(s) 241
MspI CCGG 3 cut(s) 126, 368, 487
MspR9I CCNGG 2 cut(s) 127, 400
MvaI CCWGG 1 cut(s) 400
MvnI CGCG 1 cut(s) 241
NciI CCSGG 1 cut(s) 127
NdeII GATC 3 cut(s) 3, 187, 250
NlaIII CATG 2 cut(s) 116, 312
NmuCI GTSAC 2 cut(s) 389, 437
NsbI TGCGCA 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PfeI GAWTC 1 cut(s) 453
PkrI GCNGC 1 cut(s) 243
PpuMI RGGWCCY 1 cut(s) 350
Psp1406I AACGTT 1 cut(s) 266
Psp5II RGGWCCY 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 398
PspGI CCWGG 1 cut(s) 398
PspPI GGNCC 2 cut(s) 305, 350
PspPPI RGGWCCY 1 cut(s) 350
PstNI CAGNNNCTG 1 cut(s) 353
RsaI GTAC 3 cut(s) 117, 124, 179
RsaNI GTAC 3 cut(s) 116, 123, 178
SacII CCGCGG 1 cut(s) 242
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 3 cut(s) 3, 187, 250
Sau96I GGNCC 2 cut(s) 305, 350
ScrFI CCNGG 2 cut(s) 127, 400
SetI ASST 7 cut(s) 100, 108, 164, 179, 269, 355, 428
SfaNI GCATC 1 cut(s) 81
SfcI CTRYAG 2 cut(s) 282, 385
Sfr303I CCGCGG 1 cut(s) 242
SgrBI CCGCGG 1 cut(s) 242
SinI GGWCC 2 cut(s) 305, 350
Sse9I AATT 3 cut(s) 65, 394, 457
SsiI CCGC 3 cut(s) 90, 239, 241
SspMI CTAG 2 cut(s) 32, 245
StyD4I CCNGG 2 cut(s) 125, 398
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 2 cut(s) 108, 269
TasI AATT 3 cut(s) 65, 394, 457
TatI WGTACW 1 cut(s) 115
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 1 cut(s) 453
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 2 cut(s) 389, 437
Tsp45I GTSAC 2 cut(s) 389, 437
TspDTI ATGAA 3 cut(s) 192, 418, 465
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 1 cut(s) 442
VpaK11BI GGWCC 2 cut(s) 305, 350
XspI CTAG 2 cut(s) 32, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.