pycom07g23320

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0014997
Physical Location & Seq
Forward (+)
24292075 .. 24293317
1243 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g23320.1

Sequence Viewer

Length: 621 bp
ATGCTTTTCTTTAACTCCTCCGCATCCCGCAAGTCGTCGTCGCATTCTATCTGCGGCAAATCATCAAACATGTTCGGAGCACTCTTTCTTCTTCTCCAAGTTTGCTTTCTTTTTACGCTCTCACAAGCCAAGCTAAGCATCATACGGCCGGAAGCCGCCGCCAAGGACTTCAACGTCAGCAAAATCCCGAACGCGGGGACTTGTTCTTATACGGCGATTATAACTACGAGCTGCTCCTCGACGAGGTACACGAGGGATCAGATCAGTATCTCCTTTGGGGATGCTTATGGAAATCAGATTTATGCTCCGCGACTGGATGATCCATCTTCGAGAACATTTGAACTGTGTTCTACAGACACATTCGATATATACGGCCCCTGTGCATACCAAATCTGCTACTTGTACCTCTACAGAACCGGACCGGATGGTTGGAAGCCTGAGTATGTGAAAATCTATGGTCACAATTCCAGGGCAGTTACATTTACCTACAATACCTTCATTCCCAATGATCTTTGGTACGGGTTTAACTGGTGCAACGCCGCCCCCTCTTCCGCCCAGAAATCTGCTCAGAGATGGTCTATGTTTGCCATAGCCGGGCTTATTGGTTTTCTTATATTGTAA

Protein Analysis

207

Amino Acids

23.01

Weight (kDa)

8.75

Isoelectric Point (pI)

28.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 64 - 178 3.2e-52 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62200
fragaria_vesca FvH4_7g28400
malus_domestica MD01G1187700.v1.1 MD07G1259800.v1.1
prunus_persica Prupe.2G284100_v2.0.a1
pyrus_communis pycom01g19960 pycom07g23320
rosa_chinensis RchiOBHm_Chr1g0375361
rosa_laevigata RLG00000026668
rosa_rugosa Rorug01G0390500.1 Rorug01G0390600
rosa_samantha Rh1AG401000 Rh1BG363300 Rh1CG376600 Rh1DG394100
rosa_wichuraiana Rw1G035600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 221
AasI GACNNNNNNGTC 1 cut(s) 173
AccII CGCG 2 cut(s) 194, 310
AciI CCGC 9 cut(s) 21, 28, 54, 156, 159, 194, 308, 540, 552
AclWI GGATC 2 cut(s) 264, 314
AcoI YGGCCR 1 cut(s) 146
AfaI GTAC 3 cut(s) 248, 404, 518
AfiI CCNNNNNNNGG 4 cut(s) 193, 194, 243, 594
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 2 cut(s) 172, 341
AjnI CCWGG 1 cut(s) 467
AjuI GAANNNNNNNTTGG 2 cut(s) 90, 122
AluBI AGCT 2 cut(s) 133, 231
AluI AGCT 2 cut(s) 133, 231
Alw21I GWGCWC 1 cut(s) 82
AlwI GGATC 2 cut(s) 264, 314
AoxI GGCC 2 cut(s) 146, 373
ApeKI GCWGC 1 cut(s) 231
AspS9I GGNCC 2 cut(s) 374, 419
AsuC2I CCSGG 1 cut(s) 595
AvaII GGWCC 1 cut(s) 419
BauI CACGAG 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 218
BccI CCATC 3 cut(s) 331, 419, 567
BceAI ACGGC 3 cut(s) 161, 228, 388
BciT130I CCWGG 1 cut(s) 469
BcnI CCSGG 1 cut(s) 595
BfmI CTRYAG 2 cut(s) 351, 409
BisI GCNGC 5 cut(s) 55, 156, 159, 232, 540
BlpI GCTNAGC 1 cut(s) 134
BlsI GCNGC 5 cut(s) 56, 157, 160, 233, 541
Bme1390I CCNGG 2 cut(s) 469, 595
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 2 cut(s) 374, 419
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 2 cut(s) 469, 595
BmsI GCATC 3 cut(s) 32, 147, 271
Bpu1102I GCTNAGC 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 595
BsaBI GATNNNNATC 1 cut(s) 266
BsaJI CCNNGG 2 cut(s) 162, 468
BsaWI WCCGGW 2 cut(s) 416, 421
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 194, 243, 594
Bse1I ACTGG 2 cut(s) 318, 533
Bse8I GATNNNNATC 1 cut(s) 266
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 2 cut(s) 162, 468
BseGI GGATG 4 cut(s) 23, 286, 322, 430
BseJI GATNNNNATC 1 cut(s) 266
BseLI CCNNNNNNNGG 4 cut(s) 193, 194, 243, 594
BseMII CTCAG 2 cut(s) 429, 581
BseNI ACTGG 2 cut(s) 318, 533
BseRI GAGGAG 2 cut(s) 7, 226
BseX3I CGGCCG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 218
Bsh1236I CGCG 2 cut(s) 194, 310
Bsh1285I CGRYCG 1 cut(s) 149
BshFI GGCC 2 cut(s) 148, 375
BsiEI CGRYCG 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 82
BsiSI CCGG 4 cut(s) 149, 417, 422, 594
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 4 cut(s) 193, 194, 243, 594
BsmFI GGGAC 1 cut(s) 211
BsmI GAATGC 1 cut(s) 43
BsnI GGCC 2 cut(s) 148, 375
Bsp1286I GDGCHC 1 cut(s) 82
Bsp143I GATC 4 cut(s) 256, 261, 319, 508
Bsp1720I GCTNAGC 1 cut(s) 134
BspACI CCGC 9 cut(s) 21, 28, 54, 156, 159, 194, 308, 540, 552
BspANI GGCC 2 cut(s) 148, 375
BspCNI CTCAG 2 cut(s) 430, 580
BspFNI CGCG 2 cut(s) 194, 310
BspLI GGNNCC 1 cut(s) 376
BspPI GGATC 2 cut(s) 264, 314
BsrI ACTGG 2 cut(s) 318, 533
BssECI CCNNGG 2 cut(s) 162, 468
BssMI GATC 4 cut(s) 256, 261, 319, 508
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 162
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 1 cut(s) 345
Bst6I CTCTTC 1 cut(s) 553
BstDEI CTNAG 3 cut(s) 134, 438, 567
BstENI CCTNNNNNAGG 1 cut(s) 241
BstF5I GGATG 4 cut(s) 23, 286, 322, 430
BstFNI CGCG 2 cut(s) 194, 310
BstKTI GATC 4 cut(s) 259, 264, 322, 511
BstMBI GATC 4 cut(s) 256, 261, 319, 508
BstMCI CGRYCG 1 cut(s) 149
BstNI CCWGG 1 cut(s) 469
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 467, 593
BstSFI CTRYAG 2 cut(s) 351, 409
BstUI CGCG 2 cut(s) 194, 310
BstV1I GCAGC 1 cut(s) 218
BstZI CGGCCG 1 cut(s) 146
BsuRI GGCC 2 cut(s) 148, 375
BtsCI GGATG 4 cut(s) 23, 286, 322, 430
Cfr13I GGNCC 2 cut(s) 374, 419
CpoI CGGWCCG 1 cut(s) 419
Csp6I GTAC 3 cut(s) 247, 403, 517
CspI CGGWCCG 1 cut(s) 419
CviAII CATG 1 cut(s) 70
CviJI RGCY 9 cut(s) 128, 133, 148, 155, 231, 375, 436, 593, 598
CviKI_1 RGCY 9 cut(s) 128, 133, 148, 155, 231, 375, 436, 593, 598
CviQI GTAC 3 cut(s) 247, 403, 517
DdeI CTNAG 3 cut(s) 134, 438, 567
DpnI GATC 4 cut(s) 258, 263, 321, 510
DpnII GATC 4 cut(s) 256, 261, 319, 508
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 146
EagI CGGCCG 1 cut(s) 146
Eam1104I CTCTTC 1 cut(s) 553
EarI CTCTTC 1 cut(s) 553
EciI GGCGGA 1 cut(s) 541
EclXI CGGCCG 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 419
Eco52I CGGCCG 1 cut(s) 146
EcoNI CCTNNNNNAGG 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 1 cut(s) 73
FaqI GGGAC 1 cut(s) 211
FatI CATG 1 cut(s) 69
FauI CCCGC 2 cut(s) 35, 187
Fnu4HI GCNGC 5 cut(s) 55, 156, 159, 232, 540
FokI GGATG 4 cut(s) 10, 293, 329, 437
Fsp4HI GCNGC 5 cut(s) 55, 156, 159, 232, 540
GluI GCNGC 5 cut(s) 55, 156, 159, 232, 540
HaeIII GGCC 2 cut(s) 148, 375
HapII CCGG 4 cut(s) 149, 417, 422, 594
Hin1II CATG 1 cut(s) 73
HpaII CCGG 4 cut(s) 149, 417, 422, 594
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 4 cut(s) 77, 261, 297, 570
Hpy188III TCNNGA 2 cut(s) 187, 330
Hpy8I GTNNAC 1 cut(s) 249
Hpy99I CGWCG 3 cut(s) 40, 43, 244
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 2 cut(s) 383, 534
HpyF3I CTNAG 3 cut(s) 134, 438, 567
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 1 cut(s) 73
Kzo9I GATC 4 cut(s) 256, 261, 319, 508
LmnI GCTCC 3 cut(s) 77, 239, 310
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 3 cut(s) 32, 147, 271
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 458, 475
MalI GATC 4 cut(s) 258, 263, 321, 510
MboI GATC 4 cut(s) 256, 261, 319, 508
MboII GAAGA 4 cut(s) 80, 83, 318, 540
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 6 cut(s) 28, 237, 246, 247, 416, 556
MseI TTAA 2 cut(s) 12, 525
MspI CCGG 4 cut(s) 149, 417, 422, 594
MspR9I CCNGG 2 cut(s) 469, 595
Mva1269I GAATGC 1 cut(s) 43
MvaI CCWGG 1 cut(s) 469
MvnI CGCG 2 cut(s) 194, 310
NciI CCSGG 1 cut(s) 595
NdeII GATC 4 cut(s) 256, 261, 319, 508
NlaIII CATG 1 cut(s) 73
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 458
NspI RCATGY 1 cut(s) 73
PciI ACATGT 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PctI GAATGC 1 cut(s) 43
PkrI GCNGC 5 cut(s) 56, 157, 160, 233, 541
PscI ACATGT 1 cut(s) 69
PsiI TTATAA 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 2 cut(s) 374, 419
RsaI GTAC 3 cut(s) 248, 404, 518
RsaNI GTAC 3 cut(s) 247, 403, 517
Rsr2I CGGWCCG 1 cut(s) 419
RsrII CGGWCCG 1 cut(s) 419
SaqAI TTAA 2 cut(s) 12, 525
SatI GCNGC 5 cut(s) 55, 156, 159, 232, 540
Sau3AI GATC 4 cut(s) 256, 261, 319, 508
Sau96I GGNCC 2 cut(s) 374, 419
ScrFI CCNGG 2 cut(s) 469, 595
SduI GDGCHC 1 cut(s) 82
SetI ASST 7 cut(s) 135, 177, 233, 248, 408, 488, 497
SfaNI GCATC 3 cut(s) 32, 147, 271
SfcI CTRYAG 2 cut(s) 351, 409
SinI GGWCC 1 cut(s) 419
Sse9I AATT 1 cut(s) 463
SsiI CCGC 9 cut(s) 21, 28, 54, 156, 159, 194, 308, 540, 552
StyD4I CCNGG 2 cut(s) 467, 593
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 1 cut(s) 177
TaqI TCGA 3 cut(s) 239, 329, 363
TasI AATT 1 cut(s) 463
TauI GCSGC 4 cut(s) 57, 158, 161, 542
Tru1I TTAA 2 cut(s) 12, 525
Tru9I TTAA 2 cut(s) 12, 525
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 419
XagI CCTNNNNNAGG 1 cut(s) 241
XceI RCATGY 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.