FvH4_7g29270

Ctf8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014612
Physical Location & Seq
Reverse (-)
21582618 .. 21583309
692 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29270.t1

Sequence Viewer

Length: 390 bp
ATGCAGATTAGGGTGAAGTGTAGTTGTGGAGAGAATTGTCCAGAATGGGCCATGATTGAGTTACAAGGGATGGTTGAAGTTCAGCCCCAGTTCCAAGATCGTCTCCAGAATCTCAAGATTGGTGTTCTCTGCCGGCCTTCCTCGCCGGAAACCTACACTTTCACGGTAGGGTATCATGAACTGACCGGGACGAAGATGCAGCTGAAGAAGCCGCTGTTGGTGTTGAACAAAGTCAAGGAGGGCGGTGAGGAGGGTGGTTCGGGGAGGGTGGAATTGGAAGTCATTGGAATTATCCGTCACCGGATTCTGTTCAAGACCAGACCTAAGGCTCTCATTTCCAAAGCACAGCCAACAATGAAGGACAGAACTGCTCTTGCATCAAGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ctf8

Protein Analysis

130

Amino Acids

14.47

Weight (kDa)

9.44

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ctf8 PF09696 51 - 109 7.5e-08 Ctf8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014612)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52220 AT5G52220
fragaria_vesca FvH4_7g29270
malus_domestica MD07G1267100.v1.1
prunus_persica Prupe.2G292700_v2.0.a1
pyrus_communis pycom01g20910
rosa_chinensis RchiOBHm_Chr1g0377021
rosa_laevigata RLG00000026550
rosa_multiflora Rmu_co8032820.1_g000001 Rmu_ssc0000155.1_g000028
rosa_roxburghii Rroxscaffold_4G00281540
rosa_rugosa Rorug01G0400900
rosa_samantha Rh1AG417700 Rh1BG377100 Rh1CG390900 Rh1DG407900
rosa_wichuraiana Rw1G036670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 212, 243
AcuI CTGAAG 1 cut(s) 224
AgsI TTSAA 3 cut(s) 77, 226, 313
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 107
AoxI GGCC 2 cut(s) 48, 134
ApeKI GCWGC 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 48
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 3 cut(s) 25, 257, 290
AxyI CCTNAGG 1 cut(s) 324
BbvI GCAGC 1 cut(s) 211
BccI CCATC 1 cut(s) 64
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 107
BisI GCNGC 2 cut(s) 200, 212
BlsI GCNGC 2 cut(s) 201, 213
Bme1390I CCNGG 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 187
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 2 cut(s) 186, 386
BmuI ACTGGG 1 cut(s) 82
BpmI CTGGAG 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 187
BsaWI WCCGGW 1 cut(s) 300
Bse118I RCCGGY 1 cut(s) 132
Bse1I ACTGG 1 cut(s) 88
Bse21I CCTNAGG 1 cut(s) 324
BseGI GGATG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 211
BshFI GGCC 2 cut(s) 50, 136
BsiSI CCGG 4 cut(s) 133, 146, 186, 301
BslFI GGGAC 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 2 cut(s) 50, 136
Bsp143I GATC 1 cut(s) 97
BspACI CCGC 2 cut(s) 212, 243
BspANI GGCC 2 cut(s) 50, 136
BspHI TCATGA 1 cut(s) 175
BsrFI RCCGGY 1 cut(s) 132
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 132
BssMI GATC 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 134
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 75
BstKTI GATC 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 2 cut(s) 142, 208
BstSCI CCNGG 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 211
Bsu36I CCTNAGG 1 cut(s) 324
BsuRI GGCC 2 cut(s) 50, 136
BtsCI GGATG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 134
CciI TCATGA 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 132
Cfr13I GGNCC 1 cut(s) 48
CviAII CATG 2 cut(s) 52, 176
CviJI RGCY 7 cut(s) 50, 85, 136, 202, 211, 329, 349
CviKI_1 RGCY 7 cut(s) 50, 85, 136, 202, 211, 329, 349
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 224
Eco81I CCTNAGG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 2 cut(s) 55, 179
FaiI YATR 2 cut(s) 53, 177
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 51, 175
Fnu4HI GCNGC 2 cut(s) 200, 212
FokI GGATG 1 cut(s) 82
Fsp4HI GCNGC 2 cut(s) 200, 212
GluI GCNGC 2 cut(s) 200, 212
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 2 cut(s) 50, 136
HapII CCGG 4 cut(s) 133, 146, 186, 301
Hin1II CATG 2 cut(s) 55, 179
HinfI GANTC 2 cut(s) 109, 304
HpaII CCGG 4 cut(s) 133, 146, 186, 301
HphI GGTGA 3 cut(s) 25, 257, 290
Hpy188III TCNNGA 5 cut(s) 41, 106, 115, 176, 313
HpyAV CCTTC 2 cut(s) 147, 352
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 3 cut(s) 4, 199, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 208
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 2 cut(s) 55, 179
KroI GCCGGC 1 cut(s) 132
KroNI GCCGGC 1 cut(s) 134
Kzo9I GATC 1 cut(s) 97
LpnPI CCDG 8 cut(s) 54, 101, 119, 146, 159, 199, 314, 331
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 186, 386
MaeIII GTNAC 2 cut(s) 60, 296
MalI GATC 1 cut(s) 99
MboI GATC 1 cut(s) 97
MboII GAAGA 2 cut(s) 205, 217
MluCI AATT 3 cut(s) 34, 272, 288
MnlI CCTC 5 cut(s) 151, 232, 241, 244, 258
MroNI GCCGGC 1 cut(s) 132
MspA1I CMGCKG 2 cut(s) 202, 214
MspI CCGG 4 cut(s) 133, 146, 186, 301
MspR9I CCNGG 1 cut(s) 187
MwoI GCNNNNNNNGC 2 cut(s) 142, 208
NaeI GCCGGC 1 cut(s) 134
NciI CCSGG 1 cut(s) 187
NdeII GATC 1 cut(s) 97
NgoMIV GCCGGC 1 cut(s) 132
NlaIII CATG 2 cut(s) 55, 179
NmuCI GTSAC 1 cut(s) 296
PagI TCATGA 1 cut(s) 175
PdiI GCCGGC 1 cut(s) 134
PfeI GAWTC 2 cut(s) 109, 304
PkrI GCNGC 2 cut(s) 201, 213
PspPI GGNCC 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 202
SatI GCNGC 2 cut(s) 200, 212
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 187
SetI ASST 3 cut(s) 155, 204, 325
SfaNI GCATC 2 cut(s) 186, 386
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 3 cut(s) 34, 272, 288
SsiI CCGC 2 cut(s) 212, 243
StyD4I CCNGG 1 cut(s) 185
TaaI ACNGT 1 cut(s) 166
TasI AATT 3 cut(s) 34, 272, 288
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 2 cut(s) 109, 304
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 1 cut(s) 199
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 2 cut(s) 192, 371
TspGWI ACGGA 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.