Rroxscaffold_4G00281540

Ctf8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014612
Physical Location & Seq
Forward (+)
3804420 .. 3805106
687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281540.1

Sequence Viewer

Length: 390 bp
ATGCAGATTCGGTTGAAGTGCAGTTGTGGAGAGAATTGTCCAGAATGGGCCATGGTTGAGTTACAGGGGATAGTTGAAGTTCAGCCCCAGTTCCAAGATCGTCTCCAGAATCTGAAGATTGGCCTCCTCTGCCGCCCTTCCTCGCCGGAAACCTACACATTCACCGTCGGGTATCATGAACTGACCGGGACGAAGCTGCCGCTGAAGAAGCCGCTCTTGGTGTTGAACAAAGTCAAGGATGCCGGTGAGGAGGGTAGTTCGGGGAGGGTGGAATTGGAAGTCATTGGAATCATACGTCACCGGATTCTGTTCAAGACCAGACCTAAGGCCCTAATTTCCAAAGCACAGCCTACAGCGAAGGAAAGAGGTACTCTTGCATCAAGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ctf8

Protein Analysis

129

Amino Acids

14.39

Weight (kDa)

9.44

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ctf8 PF09696 48 - 109 2.3e-07 Ctf8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014612)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52220 AT5G52220
fragaria_vesca FvH4_7g29270
malus_domestica MD07G1267100.v1.1
prunus_persica Prupe.2G292700_v2.0.a1
pyrus_communis pycom01g20910
rosa_chinensis RchiOBHm_Chr1g0377021
rosa_laevigata RLG00000026550
rosa_multiflora Rmu_co8032820.1_g000001 Rmu_ssc0000155.1_g000028
rosa_roxburghii Rroxscaffold_4G00281540
rosa_rugosa Rorug01G0400900
rosa_samantha Rh1AG417700 Rh1BG377100 Rh1CG390900 Rh1DG407900
rosa_wichuraiana Rw1G036670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 214
AciI CCGC 3 cut(s) 133, 200, 212
AcuI CTGAAG 2 cut(s) 134, 224
AfaI GTAC 1 cut(s) 370
AgsI TTSAA 4 cut(s) 16, 77, 226, 313
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 3 cut(s) 48, 121, 327
ApeKI GCWGC 1 cut(s) 196
AspS9I GGNCC 2 cut(s) 48, 328
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 3 cut(s) 154, 257, 290
AxyI CCTNAGG 1 cut(s) 324
BbvI GCAGC 1 cut(s) 183
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 351
BisI GCNGC 4 cut(s) 133, 197, 200, 212
BlsI GCNGC 4 cut(s) 134, 198, 201, 213
Bme1390I CCNGG 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 48, 328
BmrFI CCNGG 1 cut(s) 187
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 2 cut(s) 229, 386
BmuI ACTGGG 1 cut(s) 82
BpmI CTGGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 187
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 1 cut(s) 300
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 88
Bse21I CCTNAGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 2 cut(s) 116, 263
BseXI GCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 3 cut(s) 50, 123, 329
BsiSI CCGG 4 cut(s) 146, 186, 243, 301
BslFI GGGAC 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 3 cut(s) 50, 123, 329
Bsp143I GATC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 3 cut(s) 133, 200, 212
BspANI GGCC 3 cut(s) 50, 123, 329
BspHI TCATGA 1 cut(s) 175
BsrBI CCGCTC 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 166
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 2 cut(s) 129, 208
BstSCI CCNGG 1 cut(s) 185
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 183
Bsu36I CCTNAGG 1 cut(s) 324
BsuRI GGCC 3 cut(s) 50, 123, 329
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 1 cut(s) 244
CaiI CAGNNNCTG 1 cut(s) 112
CciI TCATGA 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 48, 328
Csp6I GTAC 1 cut(s) 369
CviAII CATG 2 cut(s) 52, 176
CviJI RGCY 7 cut(s) 50, 85, 123, 196, 211, 329, 349
CviKI_1 RGCY 7 cut(s) 50, 85, 123, 196, 211, 329, 349
CviQI GTAC 1 cut(s) 369
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 134, 224
Eco81I CCTNAGG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 2 cut(s) 55, 179
FaiI YATR 3 cut(s) 53, 177, 293
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 51, 175
Fnu4HI GCNGC 4 cut(s) 133, 197, 200, 212
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 4 cut(s) 133, 197, 200, 212
GluI GCNGC 4 cut(s) 133, 197, 200, 212
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 3 cut(s) 50, 123, 329
HapII CCGG 4 cut(s) 146, 186, 243, 301
Hin1II CATG 2 cut(s) 55, 179
HinfI GANTC 4 cut(s) 7, 109, 288, 304
HpaII CCGG 4 cut(s) 146, 186, 243, 301
HphI GGTGA 3 cut(s) 154, 257, 290
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 4 cut(s) 41, 106, 176, 313
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 147, 352
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 3 cut(s) 4, 21, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 208
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 2 cut(s) 55, 179
Kzo9I GATC 1 cut(s) 97
LpnPI CCDG 9 cut(s) 50, 54, 101, 119, 159, 199, 256, 314, 331
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 2 cut(s) 229, 386
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 2 cut(s) 60, 296
MalI GATC 1 cut(s) 99
MbiI CCGCTC 1 cut(s) 214
MboI GATC 1 cut(s) 97
MboII GAAGA 2 cut(s) 127, 217
MluCI AATT 3 cut(s) 34, 272, 333
MnlI CCTC 7 cut(s) 134, 137, 151, 241, 244, 258, 359
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 4 cut(s) 146, 186, 243, 301
MspR9I CCNGG 1 cut(s) 187
MwoI GCNNNNNNNGC 2 cut(s) 129, 208
NciI CCSGG 1 cut(s) 187
NcoI CCATGG 1 cut(s) 51
NdeII GATC 1 cut(s) 97
NlaIII CATG 2 cut(s) 55, 179
NmuCI GTSAC 1 cut(s) 296
PagI TCATGA 1 cut(s) 175
PfeI GAWTC 4 cut(s) 7, 109, 288, 304
PkrI GCNGC 4 cut(s) 134, 198, 201, 213
PspPI GGNCC 2 cut(s) 48, 328
PstNI CAGNNNCTG 1 cut(s) 112
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
SatI GCNGC 4 cut(s) 133, 197, 200, 212
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 2 cut(s) 48, 328
ScrFI CCNGG 1 cut(s) 187
SetI ASST 5 cut(s) 155, 198, 298, 325, 370
SfaNI GCATC 2 cut(s) 229, 386
SfcI CTRYAG 1 cut(s) 351
Sse9I AATT 3 cut(s) 34, 272, 333
SsiI CCGC 3 cut(s) 133, 200, 212
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 1 cut(s) 298
TasI AATT 3 cut(s) 34, 272, 333
TauI GCSGC 3 cut(s) 135, 202, 214
TfiI GAWTC 4 cut(s) 7, 109, 288, 304
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.