RchiOBHm_Chr1g0377021

Ctf8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014612
Physical Location & Seq
Reverse (-)
64149510 .. 64150128
619 bp
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UTR
Exon/CDS
Intron
PRQ60062

Sequence Viewer

Length: 504 bp
ATGCAGATTCGGGTGAAGTGCAGTTGTGGAGAGAATTGTCCAGAATGGGCCATGGTTGAGTTACAAGGAATGGTTGAAGTTCAGCCCCAGTTCAAAGATCGTCTCCAGAATCTGAAGATTGGCCTCCTCTGCCGCCCTTCCTCGCCGGAAACCTACACATTCACCGTCGGGTATCATGAACTGACCGGGACGAAGCTGCCGCTGAAGAAGCCGCTCTTGGTGTTGAACAAAGTCGAGGATGCCGGTGAGGAGGGTAGTTCGGGGAGGGTGGAATTGGAAGTCATTGGAATCATACGCCACCGGATTCTGTTCAAGACCAGACCTAAGGCCCTTATTTCCACCTACAGCGAAGGAAAGAGCTACTCTTGCATCAAGCAAATAGCAGAATTACAAGCAGAGCGCCATGGACTACTGGAAAAGCTTCCATCTCAAATATTTTCATGTATTTTGTGCGCTTCTTGTTGCAAAGAAGCAAGCTTTGAGTTTTGTTTCTATATAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ctf8

Protein Analysis

167

Amino Acids

18.86

Weight (kDa)

8.31

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ctf8 PF09696 48 - 109 4.3e-07 Ctf8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014612)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52220 AT5G52220
fragaria_vesca FvH4_7g29270
malus_domestica MD07G1267100.v1.1
prunus_persica Prupe.2G292700_v2.0.a1
pyrus_communis pycom01g20910
rosa_chinensis RchiOBHm_Chr1g0377021
rosa_laevigata RLG00000026550
rosa_multiflora Rmu_co8032820.1_g000001 Rmu_ssc0000155.1_g000028
rosa_roxburghii Rroxscaffold_4G00281540
rosa_rugosa Rorug01G0400900
rosa_samantha Rh1AG417700 Rh1BG377100 Rh1CG390900 Rh1DG407900
rosa_wichuraiana Rw1G036670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 214
AciI CCGC 3 cut(s) 133, 200, 212
AcuI CTGAAG 2 cut(s) 134, 224
AgsI TTSAA 4 cut(s) 77, 94, 226, 313
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 4 cut(s) 196, 360, 421, 477
AluI AGCT 4 cut(s) 196, 360, 421, 477
Alw26I GTCTC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 3 cut(s) 48, 121, 327
ApeKI GCWGC 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 2 cut(s) 402, 455
AspS9I GGNCC 2 cut(s) 48, 328
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 3 cut(s) 25, 154, 257
AxyI CCTNAGG 1 cut(s) 324
BbvI GCAGC 1 cut(s) 183
BccI CCATC 1 cut(s) 433
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 343
BfoI RGCGCY 1 cut(s) 403
BisI GCNGC 4 cut(s) 133, 197, 200, 212
BlsI GCNGC 4 cut(s) 134, 198, 201, 213
Bme1390I CCNGG 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 48, 328
BmrFI CCNGG 1 cut(s) 187
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 2 cut(s) 229, 378
BmuI ACTGGG 1 cut(s) 82
BpmI CTGGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 187
BsaJI CCNNGG 2 cut(s) 51, 403
BsaWI WCCGGW 1 cut(s) 300
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 2 cut(s) 88, 417
Bse21I CCTNAGG 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 51, 403
BseGI GGATG 1 cut(s) 244
BseNI ACTGG 2 cut(s) 88, 417
BseRI GAGGAG 2 cut(s) 116, 263
BseXI GCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 3 cut(s) 50, 123, 329
BsiSI CCGG 4 cut(s) 146, 186, 243, 301
BslFI GGGAC 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 3 cut(s) 50, 123, 329
Bsp143I GATC 1 cut(s) 97
Bsp19I CCATGG 2 cut(s) 51, 403
BspACI CCGC 3 cut(s) 133, 200, 212
BspANI GGCC 3 cut(s) 50, 123, 329
BspHI TCATGA 1 cut(s) 175
BsrBI CCGCTC 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 2 cut(s) 88, 417
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 51, 403
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 2 cut(s) 51, 403
Bst4CI ACNGT 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 475
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 2 cut(s) 51, 403
BstF5I GGATG 1 cut(s) 244
BstH2I RGCGCY 1 cut(s) 403
BstHHI GCGC 2 cut(s) 402, 455
BstKTI GATC 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 3 cut(s) 129, 208, 366
BstSCI CCNGG 1 cut(s) 185
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 183
Bsu36I CCTNAGG 1 cut(s) 324
BsuRI GGCC 3 cut(s) 50, 123, 329
BtgI CCRYGG 2 cut(s) 51, 403
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 475
CaiI CAGNNNCTG 1 cut(s) 112
CciI TCATGA 1 cut(s) 175
CfoI GCGC 2 cut(s) 402, 455
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 48, 328
CviAII CATG 4 cut(s) 52, 176, 404, 441
CviJI RGCY 9 cut(s) 50, 85, 123, 196, 211, 329, 360, 421, 477
CviKI_1 RGCY 9 cut(s) 50, 85, 123, 196, 211, 329, 360, 421, 477
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Eco130I CCWWGG 2 cut(s) 51, 403
Eco57I CTGAAG 2 cut(s) 134, 224
Eco81I CCTNAGG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 51, 403
ErhI CCWWGG 2 cut(s) 51, 403
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 4 cut(s) 55, 179, 407, 444
FaiI YATR 7 cut(s) 53, 177, 293, 405, 442, 495, 497
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 202
FatI CATG 4 cut(s) 51, 175, 403, 440
Fnu4HI GCNGC 4 cut(s) 133, 197, 200, 212
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 4 cut(s) 133, 197, 200, 212
GlaI GCGC 2 cut(s) 401, 454
GluI GCNGC 4 cut(s) 133, 197, 200, 212
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 403
HaeIII GGCC 3 cut(s) 50, 123, 329
HapII CCGG 4 cut(s) 146, 186, 243, 301
HhaI GCGC 2 cut(s) 402, 455
Hin1II CATG 4 cut(s) 55, 179, 407, 444
Hin6I GCGC 2 cut(s) 400, 453
HinP1I GCGC 2 cut(s) 400, 453
HindIII AAGCTT 2 cut(s) 419, 475
HinfI GANTC 4 cut(s) 7, 109, 288, 304
HpaII CCGG 4 cut(s) 146, 186, 243, 301
HphI GGTGA 3 cut(s) 25, 154, 257
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 4 cut(s) 41, 106, 176, 313
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 147, 344
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 4 cut(s) 4, 21, 369, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 208, 366
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 4 cut(s) 55, 179, 407, 444
HspAI GCGC 2 cut(s) 400, 453
Kzo9I GATC 1 cut(s) 97
LpnPI CCDG 9 cut(s) 54, 101, 119, 159, 199, 256, 314, 331, 398
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 2 cut(s) 229, 378
MaeIII GTNAC 1 cut(s) 60
MalI GATC 1 cut(s) 99
MbiI CCGCTC 1 cut(s) 214
MboI GATC 1 cut(s) 97
MboII GAAGA 2 cut(s) 127, 217
MluCI AATT 3 cut(s) 34, 272, 386
MnlI CCTC 7 cut(s) 134, 137, 151, 229, 241, 244, 258
MroXI GAANNNNTTC 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 4 cut(s) 146, 186, 243, 301
MspR9I CCNGG 1 cut(s) 187
MwoI GCNNNNNNNGC 3 cut(s) 129, 208, 366
NciI CCSGG 1 cut(s) 187
NcoI CCATGG 2 cut(s) 51, 403
NdeII GATC 1 cut(s) 97
NlaIII CATG 4 cut(s) 55, 179, 407, 444
PagI TCATGA 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 4 cut(s) 7, 109, 288, 304
PkrI GCNGC 4 cut(s) 134, 198, 201, 213
PspPI GGNCC 2 cut(s) 48, 328
PstNI CAGNNNCTG 1 cut(s) 112
SatI GCNGC 4 cut(s) 133, 197, 200, 212
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 2 cut(s) 48, 328
ScrFI CCNGG 1 cut(s) 187
SetI ASST 7 cut(s) 155, 198, 325, 344, 362, 423, 479
SfaNI GCATC 2 cut(s) 229, 378
SfcI CTRYAG 1 cut(s) 343
Sse9I AATT 3 cut(s) 34, 272, 386
SsiI CCGC 3 cut(s) 133, 200, 212
SspI AATATT 1 cut(s) 435
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 2 cut(s) 51, 403
TaaI ACNGT 1 cut(s) 166
TaqI TCGA 1 cut(s) 234
TasI AATT 3 cut(s) 34, 272, 386
TauI GCSGC 3 cut(s) 135, 202, 214
TfiI GAWTC 4 cut(s) 7, 109, 288, 304
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 192, 429
XmnI GAANNNNTTC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.