MD07G1267100.v1.1

Ctf8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014612
Physical Location & Seq
Reverse (-)
33506526 .. 33507257
732 bp
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UTR
Exon/CDS
Intron
MD07G1267100.v1.1.491

Sequence Viewer

Length: 417 bp
ATGCAGATCCGGGTGAGGTGCAGCTGCGGAGAGGACTGCCGGGAATGGGCGGTGGTGGAATTGCAAGGGGTGGTGGAAGTTCAGCCGCAATTTCAAGACCGCCTCCACAACCTCCACATTGGTCTCCTCTGCCGCCCTTCCTCGCCGGAAACCTACACGTTTACTATCGGCTATCACGAACTGACGGGGACGAAACTGCCCTTAAAGAAGCCGCTCTTGGTCTTGAAGAAAACGAAGCACTCCGAAGATGGTTCTGATAACGATGCTCGTGCCGCGGGGGTAGAATTGGAAGTCATCGGAATCATCCGCCACCGGATTCTGTTCAAGGCCAGGCCTATGGCCCTCATTTCCAAACCCCAACCAGCAGTGAAGAAAAGCGAGCAAGCTCCTTTTGCAGCACCTGCCCTGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ctf8

Protein Analysis

139

Amino Acids

15.42

Weight (kDa)

8.73

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ctf8 PF09696 49 - 113 1.9e-09 Ctf8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014612)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52220 AT5G52220
fragaria_vesca FvH4_7g29270
malus_domestica MD07G1267100.v1.1
prunus_persica Prupe.2G292700_v2.0.a1
pyrus_communis pycom01g20910
rosa_chinensis RchiOBHm_Chr1g0377021
rosa_laevigata RLG00000026550
rosa_multiflora Rmu_co8032820.1_g000001 Rmu_ssc0000155.1_g000028
rosa_roxburghii Rroxscaffold_4G00281540
rosa_rugosa Rorug01G0400900
rosa_samantha Rh1AG417700 Rh1BG377100 Rh1CG390900 Rh1DG407900
rosa_wichuraiana Rw1G036670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 409
Acc36I ACCTGC 1 cut(s) 409
AccBSI CCGCTC 1 cut(s) 214
AccII CGCG 1 cut(s) 275
AciI CCGC 9 cut(s) 27, 50, 86, 100, 133, 212, 273, 275, 307
AfiI CCNNNNNNNGG 1 cut(s) 46
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 3 cut(s) 95, 226, 325
AjnI CCWGG 2 cut(s) 329, 405
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 2 cut(s) 24, 386
AluI AGCT 2 cut(s) 24, 386
Alw26I GTCTC 1 cut(s) 128
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 3 cut(s) 327, 332, 339
ApeKI GCWGC 3 cut(s) 21, 24, 395
AspS9I GGNCC 1 cut(s) 340
AsuC2I CCSGG 2 cut(s) 11, 41
AsuHPI GGTGA 1 cut(s) 25
BauI CACGAG 1 cut(s) 267
BbvI GCAGC 3 cut(s) 11, 33, 407
BccI CCATC 1 cut(s) 242
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 331, 407
BcnI CCSGG 2 cut(s) 11, 41
BcoDI GTCTC 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 409
BisI GCNGC 7 cut(s) 22, 25, 86, 133, 212, 273, 396
BlsI GCNGC 7 cut(s) 23, 26, 87, 134, 213, 274, 397
Bme1390I CCNGG 4 cut(s) 11, 41, 331, 407
BmgT120I GGNCC 1 cut(s) 340
BmrFI CCNGG 4 cut(s) 11, 41, 331, 407
BmsI GCATC 1 cut(s) 253
BpuMI CCSGG 2 cut(s) 11, 41
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 273, 405
BsaWI WCCGGW 1 cut(s) 312
Bsc4I CCNNNNNNNGG 1 cut(s) 46
BseBI CCWGG 2 cut(s) 331, 407
BseDI CCNNGG 2 cut(s) 273, 405
BseGI GGATG 1 cut(s) 303
BseLI CCNNNNNNNGG 1 cut(s) 46
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 3 cut(s) 11, 33, 407
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 275
BshFI GGCC 3 cut(s) 329, 334, 341
BsiSI CCGG 4 cut(s) 10, 40, 146, 313
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 3 cut(s) 329, 334, 341
Bso31I GGTCTC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 6
BspACI CCGC 9 cut(s) 27, 50, 86, 100, 133, 212, 273, 275, 307
BspANI GGCC 3 cut(s) 329, 334, 341
BspFNI CGCG 1 cut(s) 275
BspMI ACCTGC 1 cut(s) 409
BspTNI GGTCTC 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 214
BssECI CCNNGG 2 cut(s) 273, 405
BssMI GATC 1 cut(s) 6
BssSI CACGAG 1 cut(s) 267
Bst2BI CACGAG 1 cut(s) 267
Bst2UI CCWGG 2 cut(s) 331, 407
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 380, 384
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 275
BstKTI GATC 1 cut(s) 9
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 3 cut(s) 272, 392, 401
BstNI CCWGG 2 cut(s) 331, 407
BstSCI CCNGG 4 cut(s) 9, 39, 329, 405
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 3 cut(s) 11, 33, 407
BstX2I RGATCY 1 cut(s) 6
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 3 cut(s) 329, 334, 341
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 380, 384
CaiI CAGNNNCTG 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 340
Cfr42I CCGCGG 1 cut(s) 276
CviJI RGCY 8 cut(s) 24, 85, 171, 211, 329, 334, 341, 386
CviKI_1 RGCY 8 cut(s) 24, 85, 171, 211, 329, 334, 341, 386
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EciI GGCGGA 1 cut(s) 296
Eco147I AGGCCT 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 329, 405
FaiI YATR 1 cut(s) 338
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 202
FauI CCCGC 1 cut(s) 268
Fnu4HI GCNGC 7 cut(s) 22, 25, 86, 133, 212, 273, 396
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 7 cut(s) 22, 25, 86, 133, 212, 273, 396
GluI GCNGC 7 cut(s) 22, 25, 86, 133, 212, 273, 396
HaeIII GGCC 3 cut(s) 329, 334, 341
HapII CCGG 4 cut(s) 10, 40, 146, 313
HinfI GANTC 2 cut(s) 300, 316
HpaII CCGG 4 cut(s) 10, 40, 146, 313
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 3 cut(s) 244, 256, 299
Hpy188III TCNNGA 3 cut(s) 95, 176, 223
Hpy8I GTNNAC 1 cut(s) 162
HpyAV CCTTC 1 cut(s) 147
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 4 cut(s) 4, 21, 64, 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 392, 401
HpySE526I ACGT 1 cut(s) 158
KspI CCGCGG 1 cut(s) 276
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 1 cut(s) 391
LpnPI CCDG 8 cut(s) 23, 53, 159, 316, 326, 343, 375, 392
Lsp1109I GCAGC 3 cut(s) 11, 33, 407
LweI GCATC 1 cut(s) 253
MaeII ACGT 1 cut(s) 158
MalI GATC 1 cut(s) 8
MbiI CCGCTC 1 cut(s) 214
MboI GATC 1 cut(s) 6
MboII GAAGA 3 cut(s) 238, 257, 382
MflI RGATCY 1 cut(s) 6
MluCI AATT 3 cut(s) 59, 89, 284
MnlI CCTC 7 cut(s) 9, 25, 113, 122, 137, 151, 353
MseI TTAA 1 cut(s) 203
MspA1I CMGCKG 2 cut(s) 24, 275
MspI CCGG 4 cut(s) 10, 40, 146, 313
MspR9I CCNGG 4 cut(s) 11, 41, 331, 407
MvaI CCWGG 2 cut(s) 331, 407
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 272, 392, 401
NciI CCSGG 2 cut(s) 11, 41
NdeII GATC 1 cut(s) 6
PaqCI CACCTGC 1 cut(s) 409
PceI AGGCCT 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 2 cut(s) 300, 316
PkrI GCNGC 7 cut(s) 23, 26, 87, 134, 213, 274, 397
Psp6I CCWGG 2 cut(s) 329, 405
PspGI CCWGG 2 cut(s) 329, 405
PspPI GGNCC 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 1 cut(s) 6
PvuII CAGCTG 1 cut(s) 24
SacII CCGCGG 1 cut(s) 276
SaqAI TTAA 1 cut(s) 203
SatI GCNGC 7 cut(s) 22, 25, 86, 133, 212, 273, 396
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 340
ScrFI CCNGG 4 cut(s) 11, 41, 331, 407
SetI ASST 7 cut(s) 20, 26, 114, 155, 161, 388, 403
SfaNI GCATC 1 cut(s) 253
Sfr303I CCGCGG 1 cut(s) 276
SgrBI CCGCGG 1 cut(s) 276
Sse9I AATT 3 cut(s) 59, 89, 284
SseBI AGGCCT 1 cut(s) 334
SsiI CCGC 9 cut(s) 27, 50, 86, 100, 133, 212, 273, 275, 307
StuI AGGCCT 1 cut(s) 334
StyD4I CCNGG 4 cut(s) 9, 39, 329, 405
TaiI ACGT 1 cut(s) 161
TasI AATT 3 cut(s) 59, 89, 284
TauI GCSGC 4 cut(s) 88, 135, 214, 275
TfiI GAWTC 2 cut(s) 300, 316
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TscAI CASTG 1 cut(s) 372
TseI GCWGC 3 cut(s) 21, 24, 395
TspRI CASTG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.