FvH4_7g30320

PAM68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013185
Physical Location & Seq
Reverse (-)
22118677 .. 22119869
1193 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30320.t1

Sequence Viewer

Length: 555 bp
ATGAGAACTCTGATCTTTCACTCACAACCATCTTTCCATGATTCTCAGTCTTCTCCATGGAAGCTCAAAACCCCAGCCCTCCACCCCACAAGTCTTCAAAGCCTACCACTCCCCAAAAGAACACATCATCATATTCAACCACATGCCAATTCATCAAAAGGGTTTGGCAATAACACACCAGCAGCTACCATGAAAGAAAAACCCTCAAGCAAAAACCCTAAGAATGATGATGATGATGATGATGAGATTCCGCAGGTCGTAACGGAGAGGATGCTGGTGAGGATTGTGGCGGCAGTCGGTCTGCCTCTGGCTACCGGCATTGCTTTCTTGAACGTTTTTGAATACATTAAGGAGAATCACTTGTGGGATGTGCCGGTTTGGCTTCCGTTCTTGACGACTTTGTTAACTTTTGGGACTTCAGCACTTGGGATTGCGTATGGGGCTTTGTCTACGAGTTGGGATGCAGAGAAGAAGGGTTCCATTCTTGGTTTTGAAGAAGCTCAGAAGAATTGGGTTGATATGTGGAGGGAGGAAGATGAAGGCTCCATTTCATGA

Protein Analysis

185

Amino Acids

20.54

Weight (kDa)

5.87

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 58 - 177 7.6e-42 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52780
fragaria_vesca FvH4_7g30320
malus_domestica MD01G1209300.v1.1 MD07G1279500.v1.1
prunus_persica Prupe.2G301700_v2.0.a1
pyrus_communis pycom01g21970 pycom07g25420
rosa_chinensis RchiOBHm_Chr1g0378471
rosa_laevigata RLG00000026434
rosa_multiflora Rmu_sc0008712.1_g000014 Rmu_sc0028132.1_g000001
rosa_roxburghii Rroxscaffold_4G00280440
rosa_rugosa Rorug01G0409100
rosa_samantha Rh1AG430400 Rh1BG388300 Rh1CG401700 Rh1DG419300
rosa_wichuraiana Rw1G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 449
AciI CCGC 2 cut(s) 251, 290
AclI AACGTT 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 402
AgsI TTSAA 5 cut(s) 98, 137, 331, 341, 494
AluBI AGCT 3 cut(s) 64, 185, 500
AluI AGCT 3 cut(s) 64, 185, 500
ApeKI GCWGC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 289
BbsI GAAGAC 2 cut(s) 42, 86
BbvI GCAGC 1 cut(s) 194
BccI CCATC 1 cut(s) 37
BfuAI ACCTGC 1 cut(s) 244
BglI GCCNNNNNGGC 1 cut(s) 379
BisI GCNGC 2 cut(s) 183, 291
BlsI GCNGC 2 cut(s) 184, 292
BmiI GGNNCC 2 cut(s) 478, 544
BmsI GCATC 2 cut(s) 261, 451
BpiI GAAGAC 2 cut(s) 42, 86
BpuEI CTTGAG 1 cut(s) 190
BsaJI CCNNGG 1 cut(s) 56
Bse118I RCCGGY 2 cut(s) 314, 373
Bse3DI GCAATG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 3 cut(s) 276, 373, 466
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 59, 515
BseXI GCAGC 1 cut(s) 194
BseYI CCCAGC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 315, 374
BslFI GGGAC 1 cut(s) 427
BsmFI GGGAC 1 cut(s) 427
Bsp143I GATC 1 cut(s) 12
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 2 cut(s) 251, 290
BspCNI CTCAG 2 cut(s) 58, 514
BspHI TCATGA 1 cut(s) 551
BspLI GGNNCC 2 cut(s) 478, 544
BspMI ACCTGC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 2 cut(s) 314, 373
BssAI RCCGGY 2 cut(s) 314, 373
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 56
BstDEI CTNAG 3 cut(s) 45, 219, 501
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 3 cut(s) 276, 373, 466
BstKTI GATC 1 cut(s) 15
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 2 cut(s) 379, 440
BstNSI RCATGY 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 194
BstV2I GAAGAC 2 cut(s) 42, 86
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 3 cut(s) 276, 373, 466
BveI ACCTGC 1 cut(s) 244
CciI TCATGA 1 cut(s) 551
Cfr10I RCCGGY 2 cut(s) 314, 373
CviAII CATG 5 cut(s) 38, 57, 143, 190, 552
CviJI RGCY 9 cut(s) 64, 77, 102, 185, 311, 382, 443, 500, 543
CviKI_1 RGCY 9 cut(s) 64, 77, 102, 185, 311, 382, 443, 500, 543
DdeI CTNAG 3 cut(s) 45, 219, 501
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 5 cut(s) 41, 60, 146, 193, 555
FaiI YATR 8 cut(s) 39, 58, 132, 144, 191, 438, 521, 553
FaqI GGGAC 1 cut(s) 427
FatI CATG 5 cut(s) 37, 56, 142, 189, 551
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 2 cut(s) 183, 291
FokI GGATG 3 cut(s) 283, 380, 473
Fsp4HI GCNGC 2 cut(s) 183, 291
GluI GCNGC 2 cut(s) 183, 291
GsaI CCCAGC 1 cut(s) 77
HapII CCGG 2 cut(s) 315, 374
Hin1II CATG 5 cut(s) 41, 60, 146, 193, 555
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HinfI GANTC 3 cut(s) 41, 247, 355
HpaI GTTAAC 1 cut(s) 405
HpaII CCGG 2 cut(s) 315, 374
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 2 cut(s) 405, 450
Hpy188I TCNGA 2 cut(s) 12, 504
Hpy188III TCNNGA 3 cut(s) 328, 391, 552
Hpy8I GTNNAC 2 cut(s) 405, 450
HpyAV CCTTC 2 cut(s) 466, 533
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 1 cut(s) 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 379, 440
HpyF3I CTNAG 3 cut(s) 45, 219, 501
HpySE526I ACGT 1 cut(s) 333
Hsp92II CATG 5 cut(s) 41, 60, 146, 193, 555
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 548
LpnPI CCDG 7 cut(s) 87, 192, 239, 260, 293, 328, 387
Lsp1109I GCAGC 1 cut(s) 194
LweI GCATC 2 cut(s) 261, 451
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 259
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MboII GAAGA 6 cut(s) 42, 86, 481, 506, 517, 545
MluCI AATT 2 cut(s) 148, 508
MnlI CCTC 7 cut(s) 89, 214, 261, 273, 315, 519, 523
MseI TTAA 2 cut(s) 348, 404
MspI CCGG 2 cut(s) 315, 374
MwoI GCNNNNNNNGC 2 cut(s) 379, 440
NcoI CCATGG 1 cut(s) 56
NdeII GATC 1 cut(s) 12
NlaIII CATG 5 cut(s) 41, 60, 146, 193, 555
NlaIV GGNNCC 2 cut(s) 478, 544
NspI RCATGY 1 cut(s) 146
PagI TCATGA 1 cut(s) 551
PfeI GAWTC 3 cut(s) 41, 247, 355
PkrI GCNGC 2 cut(s) 184, 292
Psp1406I AACGTT 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 73
PspN4I GGNNCC 2 cut(s) 478, 544
SaqAI TTAA 2 cut(s) 348, 404
SatI GCNGC 2 cut(s) 183, 291
Sau3AI GATC 1 cut(s) 12
SetI ASST 5 cut(s) 66, 187, 258, 336, 502
SfaNI GCATC 2 cut(s) 261, 451
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 2 cut(s) 148, 508
SsiI CCGC 2 cut(s) 251, 290
StyI CCWWGG 1 cut(s) 56
TaiI ACGT 1 cut(s) 336
TaqII GACCGA 1 cut(s) 287
TasI AATT 2 cut(s) 148, 508
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 3 cut(s) 41, 247, 355
Tru1I TTAA 2 cut(s) 348, 404
Tru9I TTAA 2 cut(s) 348, 404
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 4 cut(s) 141, 206, 540, 552
TspGWI ACGGA 2 cut(s) 278, 375
XceI RCATGY 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.