pycom01g21970

PAM68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013185
Physical Location & Seq
Reverse (-)
19752419 .. 19752973
555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21970.1

Sequence Viewer

Length: 555 bp
ATGAAAACTCTTATTTTTTCGTCCCAACCATCTCTCTATCTTCCAAGCTCTTTGCCATGGAAGCCCAAAACCCTAACCTTCCACCCCACAACCTTGCAGAACCTAGGCCACCTTAAAAGAAGTACGTACAAAACACATGCAAATGCAAAAGGCTTTGGCAGTGCGCCACCAGCCACGACAAAGGAAACGCCTACAAGCAAAAACTCAGGCAAAAATGATAACAACGACAGCGACGAGGAAGTTCCACAGGTCGTTTTCGAGAGGATGCTAGTGAGAATTATCGCCGCTGTGGGCCTGCCTTTAGCCACTGGCTTTGCGTTTTTGAAAGTTTTTGATGCGGTTAAGGAGAAGCACTTGTGGGATGTACCACTTTGGCTACCATTCTTGACGACCTTGTTGACTTTTGGGGCTTCGGCTCTTGGGATTGCGTATGGGGCATTGTCTACAAGTTGGGATGCAGAGAAAAAGGGTTCAGTTCTTGGATTGGAAGAAGCTCAAAGCAATTGGGTTGAGATGTGGAGGGTGGAAGATGAGAGTAATAATAGTAATAAATGA

Protein Analysis

185

Amino Acids

20.31

Weight (kDa)

7.93

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 55 - 174 1.2e-40 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52780
fragaria_vesca FvH4_7g30320
malus_domestica MD01G1209300.v1.1 MD07G1279500.v1.1
prunus_persica Prupe.2G301700_v2.0.a1
pyrus_communis pycom01g21970 pycom07g25420
rosa_chinensis RchiOBHm_Chr1g0378471
rosa_laevigata RLG00000026434
rosa_multiflora Rmu_sc0008712.1_g000014 Rmu_sc0028132.1_g000001
rosa_roxburghii Rroxscaffold_4G00280440
rosa_rugosa Rorug01G0409100
rosa_samantha Rh1AG430400 Rh1BG388300 Rh1CG401700 Rh1DG419300
rosa_wichuraiana Rw1G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 443
AciI CCGC 2 cut(s) 285, 338
AfaI GTAC 3 cut(s) 124, 128, 366
AgsI TTSAA 1 cut(s) 325
AluBI AGCT 2 cut(s) 48, 494
AluI AGCT 2 cut(s) 48, 494
AoxI GGCC 2 cut(s) 106, 292
AspA2I CCTAGG 1 cut(s) 103
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 1 cut(s) 292
AvrII CCTAGG 1 cut(s) 103
BccI CCATC 1 cut(s) 37
BfaI CTAG 2 cut(s) 104, 269
BisI GCNGC 1 cut(s) 285
BlnI CCTAGG 1 cut(s) 103
BlsI GCNGC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 292
BmsI GCATC 3 cut(s) 255, 325, 445
BsaAI YACGTR 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 56, 103
Bse1I ACTGG 1 cut(s) 313
BseDI CCNNGG 2 cut(s) 56, 103
BseGI GGATG 3 cut(s) 270, 367, 460
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 313
BshFI GGCC 2 cut(s) 108, 294
BslFI GGGAC 1 cut(s) 7
BsmFI GGGAC 1 cut(s) 7
BsnI GGCC 2 cut(s) 108, 294
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 2 cut(s) 285, 338
BspANI GGCC 2 cut(s) 108, 294
BspCNI CTCAG 1 cut(s) 218
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 56, 103
BssT1I CCWWGG 2 cut(s) 56, 103
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 1 cut(s) 205
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 3 cut(s) 270, 367, 460
BstHHI GCGC 1 cut(s) 166
BstMWI GCNNNNNNNGC 3 cut(s) 61, 170, 434
BstNSI RCATGY 1 cut(s) 140
BstSNI TACGTA 1 cut(s) 126
BsuRI GGCC 2 cut(s) 108, 294
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 3 cut(s) 270, 367, 460
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 166, 306
Cac8I GCNNGC 1 cut(s) 296
CfoI GCGC 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 292
Csp6I GTAC 3 cut(s) 123, 127, 365
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 2 cut(s) 57, 137
CviQI GTAC 3 cut(s) 123, 127, 365
DdeI CTNAG 1 cut(s) 205
Eco105I TACGTA 1 cut(s) 126
Eco130I CCWWGG 2 cut(s) 56, 103
EcoT14I CCWWGG 2 cut(s) 56, 103
ErhI CCWWGG 2 cut(s) 56, 103
FaeI CATG 2 cut(s) 60, 140
FaiI YATR 3 cut(s) 58, 138, 432
FaqI GGGAC 1 cut(s) 7
FatI CATG 2 cut(s) 56, 136
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 1 cut(s) 285
FokI GGATG 3 cut(s) 277, 374, 467
Fsp4HI GCNGC 1 cut(s) 285
FspBI CTAG 2 cut(s) 104, 269
GlaI GCGC 1 cut(s) 165
GluI GCNGC 1 cut(s) 285
HaeIII GGCC 2 cut(s) 108, 294
HhaI GCGC 1 cut(s) 166
Hin1II CATG 2 cut(s) 60, 140
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
Hpy166II GTNNAC 2 cut(s) 399, 444
Hpy188III TCNNGA 2 cut(s) 259, 385
Hpy8I GTNNAC 2 cut(s) 399, 444
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 1 cut(s) 88
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 4 cut(s) 97, 140, 146, 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 170, 434
HpyF3I CTNAG 1 cut(s) 205
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 2 cut(s) 60, 140
HspAI GCGC 1 cut(s) 164
LpnPI CCDG 5 cut(s) 183, 192, 233, 294, 308
LweI GCATC 3 cut(s) 255, 325, 445
MaeI CTAG 2 cut(s) 104, 269
MaeII ACGT 1 cut(s) 125
MboII GAAGA 3 cut(s) 32, 500, 539
MfeI CAATTG 1 cut(s) 502
MluCI AATT 2 cut(s) 276, 502
MnlI CCTC 3 cut(s) 229, 255, 513
MseI TTAA 2 cut(s) 114, 342
MslI CAYNNNNRTG 1 cut(s) 141
MspA1I CMGCKG 1 cut(s) 287
MunI CAATTG 1 cut(s) 502
MwoI GCNNNNNNNGC 3 cut(s) 61, 170, 434
NcoI CCATGG 1 cut(s) 56
NlaIII CATG 2 cut(s) 60, 140
NspI RCATGY 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PkrI GCNGC 1 cut(s) 286
Ppu21I YACGTR 1 cut(s) 126
PspPI GGNCC 1 cut(s) 292
RsaI GTAC 3 cut(s) 124, 128, 366
RsaNI GTAC 3 cut(s) 123, 127, 365
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 2 cut(s) 114, 342
SatI GCNGC 1 cut(s) 285
Sau96I GGNCC 1 cut(s) 292
SetI ASST 9 cut(s) 50, 80, 95, 105, 114, 128, 252, 395, 496
SfaNI GCATC 3 cut(s) 255, 325, 445
SmiMI CAYNNNNRTG 1 cut(s) 141
SnaBI TACGTA 1 cut(s) 126
Sse9I AATT 2 cut(s) 276, 502
SsiI CCGC 2 cut(s) 285, 338
SspMI CTAG 2 cut(s) 104, 269
StyI CCWWGG 2 cut(s) 56, 103
TaiI ACGT 1 cut(s) 128
TaqI TCGA 1 cut(s) 258
TasI AATT 2 cut(s) 276, 502
TauI GCSGC 1 cut(s) 287
Tru1I TTAA 2 cut(s) 114, 342
Tru9I TTAA 2 cut(s) 114, 342
TscAI CASTG 2 cut(s) 166, 313
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 166, 313
XceI RCATGY 1 cut(s) 140
XmaJI CCTAGG 1 cut(s) 103
XmiI GTMKAC 1 cut(s) 443
XspI CTAG 2 cut(s) 104, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.