MD07G1279500.v1.1

PAM68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013185
Physical Location & Seq
Reverse (-)
34384899 .. 34385212
314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1279500.v1.1.491

Sequence Viewer

Length: 291 bp
ATGCTAGTGAGAATTATCGCCGCCGTGGGGCTGCCTTTGGCCACGGGCTTTGCGTTTTTGAAAATTTTTGACGCGGTGAAGGAGAACCACTGGTGGGACGTGCCGCTTTGGCTACCATTCTTCACGACCTTGTTGACTTTTGGGACATCGGCCCTTGGGATTGCGTATGGGGCATTGTCTACAAGTTGGGATGCAGAGAAGAAGGGTTCAGTTCTTGGATTGGAAGAAGCTCAAAGCAATTGGGTTGAAATGTGGAGAAAGGAAGACGAGAGTAATAACAGTAAAAAATGA

Protein Analysis

97

Amino Acids

10.76

Weight (kDa)

5.26

Isoelectric Point (pI)

25.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 1 - 87 1.3e-32 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52780
fragaria_vesca FvH4_7g30320
malus_domestica MD01G1209300.v1.1 MD07G1279500.v1.1
prunus_persica Prupe.2G301700_v2.0.a1
pyrus_communis pycom01g21970 pycom07g25420
rosa_chinensis RchiOBHm_Chr1g0378471
rosa_laevigata RLG00000026434
rosa_multiflora Rmu_sc0008712.1_g000014 Rmu_sc0028132.1_g000001
rosa_roxburghii Rroxscaffold_4G00280440
rosa_rugosa Rorug01G0409100
rosa_samantha Rh1AG430400 Rh1BG388300 Rh1CG401700 Rh1DG419300
rosa_wichuraiana Rw1G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 179
AccII CGCG 1 cut(s) 74
AciI CCGC 3 cut(s) 21, 74, 104
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 63
AfiI CCNNNNNNNGG 2 cut(s) 27, 94
AgsI TTSAA 2 cut(s) 61, 248
AjiI CACGTC 1 cut(s) 100
AluBI AGCT 1 cut(s) 230
AluI AGCT 1 cut(s) 230
AoxI GGCC 2 cut(s) 39, 150
ApeKI GCWGC 1 cut(s) 31
ApoI RAATTY 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 88
BalI TGGCCA 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 18
BceAI ACGGC 1 cut(s) 8
BfaI CTAG 1 cut(s) 5
BglI GCCNNNNNGGC 1 cut(s) 109
BisI GCNGC 3 cut(s) 21, 32, 104
BlsI GCNGC 3 cut(s) 22, 33, 105
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 151
BmsI GCATC 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 24, 42, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 94
Bse1I ACTGG 1 cut(s) 95
BseDI CCNNGG 3 cut(s) 24, 42, 154
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 2 cut(s) 27, 94
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 18
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 41, 152
BslFI GGGAC 2 cut(s) 110, 157
BslI CCNNNNNNNGG 2 cut(s) 27, 94
BsmFI GGGAC 2 cut(s) 110, 157
BsnI GGCC 2 cut(s) 41, 152
BspACI CCGC 3 cut(s) 21, 74, 104
BspANI GGCC 2 cut(s) 41, 152
BspFNI CGCG 1 cut(s) 74
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 24, 42, 154
BssT1I CCWWGG 1 cut(s) 154
Bst4CI ACNGT 1 cut(s) 281
BstDSI CCRYGG 2 cut(s) 24, 42
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 2 cut(s) 109, 170
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 270
BsuRI GGCC 2 cut(s) 41, 152
BtgI CCRYGG 2 cut(s) 24, 42
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 88
Cfr13I GGNCC 1 cut(s) 151
CseI GACGC 1 cut(s) 80
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviJI RGCY 6 cut(s) 31, 41, 48, 112, 152, 230
CviKI_1 RGCY 6 cut(s) 31, 41, 48, 112, 152, 230
EaeI YGGCCR 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 154
FaiI YATR 1 cut(s) 168
FaqI GGGAC 2 cut(s) 110, 157
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 3 cut(s) 21, 32, 104
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 3 cut(s) 21, 32, 104
FspBI CTAG 1 cut(s) 5
GluI GCNGC 3 cut(s) 21, 32, 104
HaeIII GGCC 2 cut(s) 41, 152
HgaI GACGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 2 cut(s) 135, 180
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 2 cut(s) 135, 180
HpyAV CCTTC 2 cut(s) 73, 196
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 170
HpySE526I ACGT 1 cut(s) 99
LpnPI CCDG 1 cut(s) 76
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 181
MaeI CTAG 1 cut(s) 5
MaeII ACGT 1 cut(s) 99
MboII GAAGA 4 cut(s) 112, 211, 236, 275
MfeI CAATTG 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 3 cut(s) 12, 63, 238
MluNI TGGCCA 1 cut(s) 41
Mox20I TGGCCA 1 cut(s) 41
MscI TGGCCA 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 41
MunI CAATTG 1 cut(s) 238
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 2 cut(s) 109, 170
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PkrI GCNGC 3 cut(s) 22, 33, 105
PspPI GGNCC 1 cut(s) 151
SatI GCNGC 3 cut(s) 21, 32, 104
Sau96I GGNCC 1 cut(s) 151
SetI ASST 3 cut(s) 102, 131, 232
SfaNI GCATC 1 cut(s) 181
Sse9I AATT 3 cut(s) 12, 63, 238
SsiI CCGC 3 cut(s) 21, 74, 104
SspMI CTAG 1 cut(s) 5
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 102
TasI AATT 3 cut(s) 12, 63, 238
TauI GCSGC 2 cut(s) 23, 106
TscAI CASTG 1 cut(s) 95
TseI GCWGC 1 cut(s) 31
TspRI CASTG 1 cut(s) 95
XapI RAATTY 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 179
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.