pycom07g25420

PAM68-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013185
Physical Location & Seq
Reverse (-)
25737356 .. 25737910
555 bp
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UTR
Exon/CDS
Intron
pycom07g25420.1

Sequence Viewer

Length: 555 bp
ATGAAAACTCTGATTTTTTCCTCCCAACCATCTCTCTATCTTTCAAGCTCTTCGCCATGGAAGCCCAAAACCCTAACCTTCCACCCCAGAACCTTGCAGAACCAAAGCCACCCTAAAAGAAGTACGTACAAAACACATGCAAGTGCAAAAGGCTTTGGCAGTGAGCAACCAGCCACCACAAACGAAACGCCGACAAGCAAAAACTCCGGCAAAAATGATAACAACGATGGCGACGAGGAAGTTCCACAGGTTGTTTTCGAGAGAATGCTAGTGAGAATTATCGCCGCCGTGGGGCTGCCTTTGGCCACGGGCTTTGCGTTTTTGAAAGTTTTTGACGCGGTGAAGGAGAACCACTTGTGGGACGTGCCACTTTGGCTACCATTCTTGACGACCTTGTTGACTTTTGGGACATCGGCTCTTGGGATTGCGTATGGGGCATTGTCTACAAGTTGGGATGCAGAGAAGAAGGGTTCAGTTCTTGGATTGGAAGAAGCTCAAAGCAATTGGGTTGAGATGTGGAGGGAGGAAGACGAGAGTAATAATAGTAATAAATGA

Protein Analysis

185

Amino Acids

20.42

Weight (kDa)

6.09

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 39 - 175 5.8e-41 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52780
fragaria_vesca FvH4_7g30320
malus_domestica MD01G1209300.v1.1 MD07G1279500.v1.1
prunus_persica Prupe.2G301700_v2.0.a1
pyrus_communis pycom01g21970 pycom07g25420
rosa_chinensis RchiOBHm_Chr1g0378471
rosa_laevigata RLG00000026434
rosa_multiflora Rmu_sc0008712.1_g000014 Rmu_sc0028132.1_g000001
rosa_roxburghii Rroxscaffold_4G00280440
rosa_rugosa Rorug01G0409100
rosa_samantha Rh1AG430400 Rh1BG388300 Rh1CG401700 Rh1DG419300
rosa_wichuraiana Rw1G037700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 443
AccII CGCG 1 cut(s) 338
AciI CCGC 2 cut(s) 285, 338
AcoI YGGCCR 1 cut(s) 303
AfaI GTAC 2 cut(s) 124, 128
AfiI CCNNNNNNNGG 2 cut(s) 291, 358
AgsI TTSAA 2 cut(s) 45, 325
AjiI CACGTC 1 cut(s) 364
AluBI AGCT 2 cut(s) 48, 494
AluI AGCT 2 cut(s) 48, 494
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 352
BalI TGGCCA 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 534
BbvI GCAGC 1 cut(s) 282
BccI CCATC 2 cut(s) 37, 221
BceAI ACGGC 1 cut(s) 272
BfaI CTAG 1 cut(s) 269
BglI GCCNNNNNGGC 1 cut(s) 373
BisI GCNGC 2 cut(s) 285, 296
BlsI GCNGC 2 cut(s) 286, 297
BmgBI CACGTC 1 cut(s) 364
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 56, 288, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 358
BseDI CCNNGG 3 cut(s) 56, 288, 306
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 2 cut(s) 291, 358
BseXI GCAGC 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 1 cut(s) 305
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 374, 421
BslI CCNNNNNNNGG 2 cut(s) 291, 358
BsmFI GGGAC 2 cut(s) 374, 421
BsmI GAATGC 1 cut(s) 270
BsnI GGCC 1 cut(s) 305
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 2 cut(s) 285, 338
BspANI GGCC 1 cut(s) 305
BspFNI CGCG 1 cut(s) 338
BspQI GCTCTTC 1 cut(s) 55
BssECI CCNNGG 3 cut(s) 56, 288, 306
BssT1I CCWWGG 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 55
BstBAI YACGTR 1 cut(s) 126
BstDSI CCRYGG 3 cut(s) 56, 288, 306
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 1 cut(s) 338
BstMWI GCNNNNNNNGC 3 cut(s) 61, 373, 434
BstNSI RCATGY 1 cut(s) 140
BstSNI TACGTA 1 cut(s) 126
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 534
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 3 cut(s) 56, 288, 306
BtrI CACGTC 1 cut(s) 364
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 166
CseI GACGC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 123, 127
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 2 cut(s) 57, 137
CviQI GTAC 2 cut(s) 123, 127
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco105I TACGTA 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 2 cut(s) 60, 140
FaiI YATR 3 cut(s) 58, 138, 432
FaqI GGGAC 2 cut(s) 374, 421
FatI CATG 2 cut(s) 56, 136
FblI GTMKAC 1 cut(s) 443
Fnu4HI GCNGC 2 cut(s) 285, 296
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 2 cut(s) 285, 296
FspBI CTAG 1 cut(s) 269
GluI GCNGC 2 cut(s) 285, 296
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 344
Hin1II CATG 2 cut(s) 60, 140
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 2 cut(s) 399, 444
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 2 cut(s) 259, 385
Hpy8I GTNNAC 2 cut(s) 399, 444
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 3 cut(s) 88, 337, 460
HpyCH4IV ACGT 2 cut(s) 125, 363
HpyCH4V TGCA 4 cut(s) 97, 140, 146, 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 373, 434
HpySE526I ACGT 2 cut(s) 125, 363
Hsp92II CATG 2 cut(s) 60, 140
LguI GCTCTTC 1 cut(s) 55
LpnPI CCDG 4 cut(s) 100, 183, 220, 233
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 269
MaeII ACGT 2 cut(s) 125, 363
MboII GAAGA 4 cut(s) 42, 475, 500, 539
MfeI CAATTG 1 cut(s) 502
MlsI TGGCCA 1 cut(s) 305
MluCI AATT 2 cut(s) 276, 502
MluNI TGGCCA 1 cut(s) 305
MnlI CCTC 4 cut(s) 31, 229, 513, 517
Mox20I TGGCCA 1 cut(s) 305
MscI TGGCCA 1 cut(s) 305
MslI CAYNNNNRTG 1 cut(s) 141
Msp20I TGGCCA 1 cut(s) 305
MspI CCGG 1 cut(s) 207
MunI CAATTG 1 cut(s) 502
Mva1269I GAATGC 1 cut(s) 270
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 3 cut(s) 61, 373, 434
NcoI CCATGG 1 cut(s) 56
NlaIII CATG 2 cut(s) 60, 140
NspI RCATGY 1 cut(s) 140
PciSI GCTCTTC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 314
PctI GAATGC 1 cut(s) 270
PkrI GCNGC 2 cut(s) 286, 297
Ppu21I YACGTR 1 cut(s) 126
RsaI GTAC 2 cut(s) 124, 128
RsaNI GTAC 2 cut(s) 123, 127
RseI CAYNNNNRTG 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 55
SatI GCNGC 2 cut(s) 285, 296
SetI ASST 8 cut(s) 50, 80, 95, 128, 252, 366, 395, 496
SfaNI GCATC 1 cut(s) 445
SmiMI CAYNNNNRTG 1 cut(s) 141
SnaBI TACGTA 1 cut(s) 126
Sse9I AATT 2 cut(s) 276, 502
SsiI CCGC 2 cut(s) 285, 338
SspMI CTAG 1 cut(s) 269
StyI CCWWGG 1 cut(s) 56
TaiI ACGT 2 cut(s) 128, 366
TaqI TCGA 1 cut(s) 258
TasI AATT 2 cut(s) 276, 502
TauI GCSGC 1 cut(s) 287
TscAI CASTG 1 cut(s) 166
TseI GCWGC 1 cut(s) 295
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 166
XceI RCATGY 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 443
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.