FvH4_7g31820

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004702
Physical Location & Seq
Forward (+)
22959282 .. 22964046
4765 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31820.t1

Sequence Viewer

Length: 1110 bp
ATGGGGGAAGACGCAGGAAGAGGTTTGGAATGCCAGAAGACTATGGATGGGAAGGGGAGCAACAGAAACGGTTCAGAGAAGGCCATCCCTTCTTGTTGCTTGAAGGCTAGGGCTTCAGACCCTGAGGCTGATGCAATATGCCATTCTACTGTTGTTTCTGGGTGGTTTTCAGAATCCCAATCTCGCGCAGATAAGGCTAGTAAAAAGACTTACTTCAACAATCCGATGTGGCCTGGTGAAGCCCACTCATTGAAAGTAGAAAATATTCTATACAAGGGAAAATCAGAGTTCCAAGAGGTTTTGGTTTTCGAGTCTTCAACATATGGAAAAGTGCTTGTTCTTGATGGCATTATTCAGCTGAGTGAGAAGGATGAATGTGCATACCAGGAGATGATAACTCATCTACCTCTTTGTTCGATTCCCTCCCCCAAAAATGTTCTGGTTGTGGGTGGTGGTGATGGCGGAGTTCTTAGGGAGATTTCGCGCCATTCGTCTGTTGAGCATATTGATATATGTGAGATAGATCAGTTGGTTATAGATGTGTCTAAAAAGTTTTTTCCAGAGTTAGCTGTTGGATTTGAGGACCCTCGTGTCCATCTTCATGTCGGTGATGCTACTGAATTCCTAAGGAATGCACCTAAAGGGAAATATGATGCTGTAATTGTTGATTCATCAGATCCTGTAGGTCCTGCTCAGGAGCTTGTAGAGAAGCCATTTTTTGAGACAGTAGCTAGAGCATTAAGGCCTGGCGGTGTTCTATGTAACATGGCAGAGAGTATGTGGCTCCATACACATCTTATTGAGGATATGATCTCTGTTTGCCAGCTAACATTCAAGGGGTCAGTTAACTATGCATGGACGAGTGTTCCCACATATCCAAGTGGTGTTATAGGTTTTCTGCTATGTTCAACAGAAGGGCCTCCTGTTGATTTTAAAAATCCAGTCAATTCCATTGAGAAATTGGCAGGAGCTGTCAACCACAAAAGAGAACTTCGGTTCTATAACTCTGAGATGCACTCAGCTGCCTTTGCCTTGCCTGCATTTTTGAGGAGGGAGGTGAGCGCACTCCATGATTCTTCAAATCCAGCAAGACGAAATGGCTCGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

370

Amino Acids

40.41

Weight (kDa)

5.68

Isoelectric Point (pI)

50.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synt_N PF17284 75 - 122 6.6e-20 Spermidine synthase tetramerisation domain
Spermine_synth PF01564 125 - 313 8.8e-71 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 590
AccII CGCG 2 cut(s) 186, 484
AciI CCGC 2 cut(s) 462, 750
AclWI GGATC 1 cut(s) 671
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 99
AgsI TTSAA 7 cut(s) 103, 217, 253, 318, 835, 909, 1080
AjnI CCWGG 3 cut(s) 232, 384, 745
AluBI AGCT 7 cut(s) 358, 569, 700, 731, 826, 971, 1022
AluI AGCT 7 cut(s) 358, 569, 700, 731, 826, 971, 1022
Alw26I GTCTC 1 cut(s) 716
AlwI GGATC 1 cut(s) 671
AlwNI CAGNNNCTG 3 cut(s) 122, 680, 971
AoxI GGCC 4 cut(s) 81, 230, 743, 917
ApeKI GCWGC 1 cut(s) 1022
ApoI RAATTY 1 cut(s) 620
Asp700I GAANNNNTTC 2 cut(s) 70, 264
AspLEI GCGC 3 cut(s) 188, 486, 1064
AspS9I GGNCC 3 cut(s) 583, 686, 917
AsuHPI GGTGA 4 cut(s) 248, 467, 620, 1069
AvaII GGWCC 2 cut(s) 583, 686
AxyI CCTNAGG 2 cut(s) 123, 626
BauI CACGAG 1 cut(s) 588
BbsI GAAGAC 3 cut(s) 15, 44, 306
BbvI GCAGC 1 cut(s) 1009
BccI CCATC 5 cut(s) 41, 92, 338, 452, 603
BciT130I CCWGG 3 cut(s) 234, 386, 747
BcoDI GTCTC 1 cut(s) 716
BfaI CTAG 4 cut(s) 108, 198, 732, 1108
BfmI CTRYAG 1 cut(s) 681
BisI GCNGC 1 cut(s) 1023
BlsI GCNGC 1 cut(s) 1024
Bme1390I CCNGG 3 cut(s) 234, 386, 747
Bme18I GGWCC 2 cut(s) 583, 686
BmgT120I GGNCC 3 cut(s) 583, 686, 917
BmiI GGNNCC 2 cut(s) 585, 785
BmrFI CCNGG 3 cut(s) 234, 386, 747
BmsI GCATC 4 cut(s) 121, 601, 643, 1002
BpiI GAAGAC 3 cut(s) 15, 44, 306
BplI GAGNNNNNCTC 2 cut(s) 1001, 1033
Bpu10I CCTNAGC 1 cut(s) 693
Bse1I ACTGG 1 cut(s) 941
Bse21I CCTNAGG 2 cut(s) 123, 626
BseBI CCWGG 3 cut(s) 234, 386, 747
BseGI GGATG 3 cut(s) 52, 84, 376
BseMII CTCAG 5 cut(s) 114, 350, 707, 999, 1032
BseNI ACTGG 1 cut(s) 941
BseRI GAGGAG 1 cut(s) 1063
BseXI GCAGC 1 cut(s) 1009
Bsh1236I CGCG 2 cut(s) 186, 484
BshFI GGCC 4 cut(s) 83, 232, 745, 919
BsmAI GTCTC 1 cut(s) 716
BsmI GAATGC 2 cut(s) 35, 637
BsnI GGCC 4 cut(s) 83, 232, 745, 919
Bsp143I GATC 3 cut(s) 523, 676, 810
BspACI CCGC 2 cut(s) 462, 750
BspANI GGCC 4 cut(s) 83, 232, 745, 919
BspCNI CTCAG 5 cut(s) 115, 351, 706, 1000, 1031
BspFNI CGCG 2 cut(s) 186, 484
BspLI GGNNCC 2 cut(s) 585, 785
BspPI GGATC 1 cut(s) 671
BsrI ACTGG 1 cut(s) 941
BssMI GATC 3 cut(s) 523, 676, 810
BssSI CACGAG 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 3 cut(s) 234, 386, 747
Bst4CI ACNGT 3 cut(s) 71, 151, 727
Bst6I CTCTTC 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 824, 1038
BstDEI CTNAG 7 cut(s) 123, 359, 470, 626, 693, 1008, 1018
BstF5I GGATG 3 cut(s) 52, 84, 376
BstFNI CGCG 2 cut(s) 186, 484
BstHHI GCGC 3 cut(s) 188, 486, 1064
BstKTI GATC 3 cut(s) 526, 679, 813
BstMAI GTCTC 1 cut(s) 716
BstMBI GATC 3 cut(s) 523, 676, 810
BstMWI GCNNNNNNNGC 3 cut(s) 194, 1028, 1037
BstNI CCWGG 3 cut(s) 234, 386, 747
BstSCI CCNGG 3 cut(s) 232, 384, 745
BstSFI CTRYAG 1 cut(s) 681
BstUI CGCG 2 cut(s) 186, 484
BstV1I GCAGC 1 cut(s) 1009
BstV2I GAAGAC 3 cut(s) 15, 44, 306
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
Bsu36I CCTNAGG 2 cut(s) 123, 626
BsuRI GGCC 4 cut(s) 83, 232, 745, 919
BtsCI GGATG 3 cut(s) 52, 84, 376
Cac8I GCNNGC 2 cut(s) 824, 1038
CaiI CAGNNNCTG 3 cut(s) 122, 680, 971
CfoI GCGC 3 cut(s) 188, 486, 1064
Cfr13I GGNCC 3 cut(s) 583, 686, 917
CseI GACGC 1 cut(s) 20
CviAII CATG 4 cut(s) 602, 766, 855, 1070
DdeI CTNAG 7 cut(s) 123, 359, 470, 626, 693, 1008, 1018
DpnI GATC 3 cut(s) 525, 678, 812
DpnII GATC 3 cut(s) 523, 676, 810
DraI TTTAAA 1 cut(s) 934
DrdI GACNNNNNNGTC 1 cut(s) 590
DseDI GACNNNNNNGTC 1 cut(s) 590
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
EciI GGCGGA 1 cut(s) 477
Eco147I AGGCCT 1 cut(s) 745
Eco47I GGWCC 2 cut(s) 583, 686
Eco57I CTGAAG 1 cut(s) 99
Eco81I CCTNAGG 2 cut(s) 123, 626
EcoO109I RGGNCCY 3 cut(s) 583, 686, 917
EcoRI GAATTC 1 cut(s) 620
EcoRII CCWGG 3 cut(s) 232, 384, 745
EcoT22I ATGCAT 1 cut(s) 856
FaeI CATG 4 cut(s) 605, 769, 858, 1073
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 4 cut(s) 601, 765, 854, 1069
FauNDI CATATG 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 1023
FokI GGATG 3 cut(s) 59, 71, 383
Fsp4HI GCNGC 1 cut(s) 1023
FspBI CTAG 4 cut(s) 108, 198, 732, 1108
GlaI GCGC 3 cut(s) 187, 485, 1063
GluI GCNGC 1 cut(s) 1023
HaeIII GGCC 4 cut(s) 83, 232, 745, 919
HgaI GACGC 1 cut(s) 20
HhaI GCGC 3 cut(s) 188, 486, 1064
Hin1II CATG 4 cut(s) 605, 769, 858, 1073
Hin6I GCGC 3 cut(s) 186, 484, 1062
HinP1I GCGC 3 cut(s) 186, 484, 1062
HincII GTYRAC 2 cut(s) 847, 976
HindII GTYRAC 2 cut(s) 847, 976
HinfI GANTC 5 cut(s) 173, 311, 418, 668, 1073
HpaI GTTAAC 1 cut(s) 847
HphI GGTGA 4 cut(s) 248, 467, 620, 1069
Hpy166II GTNNAC 2 cut(s) 847, 976
Hpy188I TCNGA 7 cut(s) 76, 118, 172, 225, 286, 676, 1009
Hpy188III TCNNGA 3 cut(s) 341, 560, 695
Hpy8I GTNNAC 2 cut(s) 847, 976
HpyAV CCTTC 6 cut(s) 46, 73, 97, 99, 361, 908
HpyCH4III ACNGT 3 cut(s) 71, 151, 727
HpyCH4V TGCA 6 cut(s) 134, 380, 635, 854, 1015, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 1028, 1037
HpyF3I CTNAG 7 cut(s) 123, 359, 470, 626, 693, 1008, 1018
Hsp92II CATG 4 cut(s) 605, 769, 858, 1073
HspAI GCGC 3 cut(s) 186, 484, 1062
KspAI GTTAAC 1 cut(s) 847
Kzo9I GATC 3 cut(s) 523, 676, 810
LmnI GCTCC 4 cut(s) 57, 697, 789, 968
Lsp1109I GCAGC 1 cut(s) 1009
LweI GCATC 4 cut(s) 121, 601, 643, 1002
MaeI CTAG 4 cut(s) 108, 198, 732, 1108
MaeIII GTNAC 1 cut(s) 761
MalI GATC 3 cut(s) 525, 678, 812
MboI GATC 3 cut(s) 523, 676, 810
MboII GAAGA 6 cut(s) 20, 30, 49, 306, 590, 1068
MflI RGATCY 1 cut(s) 676
MluCI AATT 4 cut(s) 620, 660, 946, 959
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 553
Mph1103I ATGCAT 1 cut(s) 856
MroXI GAANNNNTTC 2 cut(s) 70, 264
MseI TTAA 3 cut(s) 740, 846, 933
MslI CAYNNNNRTG 2 cut(s) 600, 606
MspA1I CMGCKG 2 cut(s) 358, 1022
MspR9I CCNGG 3 cut(s) 234, 386, 747
Mva1269I GAATGC 2 cut(s) 35, 637
MvaI CCWGG 3 cut(s) 234, 386, 747
MvnI CGCG 2 cut(s) 186, 484
MwoI GCNNNNNNNGC 3 cut(s) 194, 1028, 1037
NdeI CATATG 1 cut(s) 322
NdeII GATC 3 cut(s) 523, 676, 810
NlaIII CATG 4 cut(s) 605, 769, 858, 1073
NlaIV GGNNCC 2 cut(s) 585, 785
NsiI ATGCAT 1 cut(s) 856
PceI AGGCCT 1 cut(s) 745
PcsI WCGNNNNNNNCGW 1 cut(s) 488
PctI GAATGC 2 cut(s) 35, 637
PdmI GAANNNNTTC 2 cut(s) 70, 264
PfeI GAWTC 4 cut(s) 173, 418, 668, 1073
PkrI GCNGC 1 cut(s) 1024
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PpuMI RGGWCCY 2 cut(s) 583, 686
Psp5II RGGWCCY 2 cut(s) 583, 686
Psp6I CCWGG 3 cut(s) 232, 384, 745
PspGI CCWGG 3 cut(s) 232, 384, 745
PspN4I GGNNCC 2 cut(s) 585, 785
PspPI GGNCC 3 cut(s) 583, 686, 917
PspPPI RGGWCCY 2 cut(s) 583, 686
PstNI CAGNNNCTG 3 cut(s) 122, 680, 971
PsuI RGATCY 1 cut(s) 676
PvuII CAGCTG 2 cut(s) 358, 1022
RseI CAYNNNNRTG 2 cut(s) 600, 606
SaqAI TTAA 3 cut(s) 740, 846, 933
SatI GCNGC 1 cut(s) 1023
Sau3AI GATC 3 cut(s) 523, 676, 810
Sau96I GGNCC 3 cut(s) 583, 686, 917
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 3 cut(s) 234, 386, 747
SfaNI GCATC 4 cut(s) 121, 601, 643, 1002
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 2 cut(s) 583, 686
SmiMI CAYNNNNRTG 2 cut(s) 600, 606
Sse9I AATT 4 cut(s) 620, 660, 946, 959
SseBI AGGCCT 1 cut(s) 745
SsiI CCGC 2 cut(s) 462, 750
SspI AATATT 1 cut(s) 265
SspMI CTAG 4 cut(s) 108, 198, 732, 1108
StuI AGGCCT 1 cut(s) 745
StyD4I CCNGG 3 cut(s) 232, 384, 745
TaaI ACNGT 3 cut(s) 71, 151, 727
TaqI TCGA 2 cut(s) 309, 416
TasI AATT 4 cut(s) 620, 660, 946, 959
TfiI GAWTC 4 cut(s) 173, 418, 668, 1073
Tru1I TTAA 3 cut(s) 740, 846, 933
Tru9I TTAA 3 cut(s) 740, 846, 933
TseI GCWGC 1 cut(s) 1022
TspDTI ATGAA 3 cut(s) 387, 590, 660
VpaK11BI GGWCC 2 cut(s) 583, 686
XapI RAATTY 1 cut(s) 620
XcmI CCANNNNNNNNNTGG 2 cut(s) 436, 958
XmnI GAANNNNTTC 2 cut(s) 70, 264
XspI CTAG 4 cut(s) 108, 198, 732, 1108
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.