Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004702

Physical Location & Seq

Genomic Coordinates Forward (+)

31308216 .. 31313091

Gene Structure 4876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1221000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGAGGACGGCGCAGGAAGAGGTTTGGAATGCCAGAAGACTATGGATGGGAAGGGGAGTAACGGGAATGGTTCGGAGAAGGCCATCCCTTCTTGTTGCTTGAAGGCTATGGCTTCTACCCCTGAGGCCGAGGCAAAATGTCATTCCACTGTTGTTTCTGGGTGGTTCTCAGAATCCCAGTCTCGCTCTGAGAAGGATTGTAAAAAGGTTTATTTCAACAACCCTATGTGGCCAGGTGAAGCTCATTCACTGAAAGTAGAAAATATTCTGTATAGGGGAAAATCAGAGTACCAAGAGATTTTGGTGTTTGAGTCCTCAATATATGGAAAAGTGCTTGTTCTTGATGGCATTATCCAGCTGAGTGAGAAAGATGAATGTGCATACCAGGAGATGATAGCACATCTACCTCTTTGTTCAATTTCCTCCCCCAAGACAGTTCTGGTTGTTGGTGGTGGTGATGGTGGGGTTCTTAGGGAGGTTTCTCGCCATCCTTCCATTGAGCATATTGATATATGTGAGATAGATAAGATGGTTATAGATGTAAGTGGTGTGTCCAAGAAGTTTTTCCCTCAGTTAGCCGTTGGATTTCAGGACCCTCGCGTCCACCTTCATGTCGGTGATGCTACTGAATTCTTAAGGCTTGCACCTGAAGGGAAGTATGATGCTGTAATTGTTGATTCGTCAGACCCTGTCGGTCCTGCCCAAGAGCTTGTAGAGAAGCCATTTTTTGAGACAATAGCTAGAGCATTAAGGCCTGGTGGTGTTCTCTGTAATATGACAGAGAGCATGTGGCTCCATACGCATCTTATTCAAGATATGATCTCTGTTTGCCGTCAAATATTCAAGGGGTCTGTCAATTATGCTTGGGCGAGTGTTCCTACGTATCCAAGTGGTGTGATAGGTTTTCTGTTGTGCTCAACAGCGGGGCCTCCCGTTGATTTCAAAAATCCCGTCAATTCTATTGAGAAGTTAGAAAGAGCTCTCAAACATAAGAGAGAACTTAAGTTCTATAACTCCGAGATGCACTCAGCTGCCTTTGCGTTGCCTCCTTTCTTGAGGAGGGAGGTGAGTGCACTACGTGAGTCTTCAACACCGGCAAGACAAATCGGCGAGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

373

Amino Acids

40.94

Weight (kDa)

6.03

Isoelectric Point (pI)

51.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	75 - 122	2.2e-19	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	125 - 316	6.9e-68	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004702)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53120 AT5G53120 AT5G53120 AT5G53120 AT5G53120 AT5G53120 AT5G53120
fragaria_vesca	FvH4_7g31820 FvH4_7g31820
malus_domestica	MD01G1221000.v1.1 MD07G1291100.v1.1
prunus_persica	Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1
pyrus_communis	pycom01g23040 pycom07g26370
rosa_chinensis	RchiOBHm_Chr1g0381051
rosa_laevigata	RLG00000026249
rosa_multiflora	Rmu_sc0004494.1_g000004
rosa_roxburghii	Rroxscaffold_4G00278820
rosa_rugosa	Rorug01G0426900
rosa_samantha	Rh1CG420800
rosa_wichuraiana	Rw1G039270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	599
AccII	CGCG	1 cut(s)	600
AciI	CCGC	1 cut(s)	923
AcoI	YGGCCR	1 cut(s)	230
AcsI	RAATTY	1 cut(s)	629
AcuI	CTGAAG	1 cut(s)	669
AdeI	CACNNNGTG	1 cut(s)	1079
AfaI	GTAC	1 cut(s)	290
AflII	CTTAAG	2 cut(s)	634, 1001
AgsI	TTSAA	7 cut(s)	103, 217, 417, 812, 844, 943, 1089
AjnI	CCWGG	3 cut(s)	232, 384, 754
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AluBI	AGCT	6 cut(s)	242, 358, 709, 740, 980, 1031
AluI	AGCT	6 cut(s)	242, 358, 709, 740, 980, 1031
Alw21I	GWGCWC	3 cut(s)	917, 982, 1075
Alw26I	GTCTC	2 cut(s)	186, 725
Alw44I	GTGCAC	1 cut(s)	1071
AlwNI	CAGNNNCTG	1 cut(s)	689
AoxI	GGCC	5 cut(s)	81, 126, 230, 752, 926
ApaLI	GTGCAC	1 cut(s)	1071
ApeKI	GCWGC	1 cut(s)	1031
ApoI	RAATTY	1 cut(s)	629
ArsI	GACNNNNNNTTYG	2 cut(s)	936, 968
Asp700I	GAANNNNTTC	2 cut(s)	264, 563
AspLEI	GCGC	1 cut(s)	14
AspS9I	GGNCC	3 cut(s)	592, 695, 926
AsuHPI	GGTGA	4 cut(s)	248, 467, 629, 1078
AvaII	GGWCC	2 cut(s)	592, 695
AxyI	CCTNAGG	1 cut(s)	123
BaeGI	GKGCMC	1 cut(s)	1075
BalI	TGGCCA	1 cut(s)	232
BanII	GRGCYC	1 cut(s)	982
BbsI	GAAGAC	2 cut(s)	44, 1077
Bbv12I	GWGCWC	3 cut(s)	917, 982, 1075
BbvI	GCAGC	1 cut(s)	1018
BccI	CCATC	6 cut(s)	41, 92, 338, 452, 495, 523
BceAI	ACGGC	3 cut(s)	25, 563, 816
BciT130I	CCWGG	3 cut(s)	234, 386, 756
BciVI	GTATCC	1 cut(s)	894
BcoDI	GTCTC	2 cut(s)	186, 725
BfaI	CTAG	1 cut(s)	741
BfrI	CTTAAG	2 cut(s)	634, 1001
BfuI	GTATCC	1 cut(s)	894
BisI	GCNGC	1 cut(s)	1032
BlsI	GCNGC	1 cut(s)	1033
Bme1390I	CCNGG	3 cut(s)	234, 386, 756
Bme18I	GGWCC	2 cut(s)	592, 695
BmgT120I	GGNCC	3 cut(s)	592, 695, 926
BmiI	GGNNCC	3 cut(s)	594, 794, 927
BmrFI	CCNGG	3 cut(s)	234, 386, 756
BmrI	ACTGGG	1 cut(s)	172
BmsI	GCATC	4 cut(s)	610, 652, 811, 1011
BmuI	ACTGGG	1 cut(s)	172
BpiI	GAAGAC	2 cut(s)	44, 1077
BplI	GAGNNNNNCTC	2 cut(s)	1010, 1042
BpuEI	CTTGAG	1 cut(s)	1075
BsaAI	YACGTR	2 cut(s)	882, 1079
BsaJI	CCNNGG	1 cut(s)	129
Bse118I	RCCGGY	1 cut(s)	1093
Bse1I	ACTGG	1 cut(s)	178
Bse21I	CCTNAGG	1 cut(s)	123
BseBI	CCWGG	3 cut(s)	234, 386, 756
BseDI	CCNNGG	1 cut(s)	129
BseGI	GGATG	3 cut(s)	52, 84, 487
BseMII	CTCAG	6 cut(s)	114, 180, 183, 350, 584, 1041
BseNI	ACTGG	1 cut(s)	178
BseRI	GAGGAG	1 cut(s)	1072
BseSI	GKGCMC	1 cut(s)	1075
BseXI	GCAGC	1 cut(s)	1018
Bsh1236I	CGCG	1 cut(s)	600
BshFI	GGCC	5 cut(s)	83, 128, 232, 754, 928
BsiHKAI	GWGCWC	3 cut(s)	917, 982, 1075
BsiSI	CCGG	1 cut(s)	1094
BsmAI	GTCTC	2 cut(s)	186, 725
BsmI	GAATGC	1 cut(s)	35
BsnI	GGCC	5 cut(s)	83, 128, 232, 754, 928
Bsp1286I	GDGCHC	3 cut(s)	917, 982, 1075
Bsp143I	GATC	1 cut(s)	819
BspACI	CCGC	1 cut(s)	923
BspANI	GGCC	5 cut(s)	83, 128, 232, 754, 928
BspCNI	CTCAG	6 cut(s)	115, 181, 182, 351, 583, 1040
BspFNI	CGCG	1 cut(s)	600
BspLI	GGNNCC	3 cut(s)	594, 794, 927
BspTI	CTTAAG	2 cut(s)	634, 1001
BsrFI	RCCGGY	1 cut(s)	1093
BsrI	ACTGG	1 cut(s)	178
BssAI	RCCGGY	1 cut(s)	1093
BssECI	CCNNGG	1 cut(s)	129
BssMI	GATC	1 cut(s)	819
Bst2UI	CCWGG	3 cut(s)	234, 386, 756
Bst4CI	ACNGT	2 cut(s)	151, 436
Bst6I	CTCTTC	1 cut(s)	13
BstAFI	CTTAAG	2 cut(s)	634, 1001
BstBAI	YACGTR	2 cut(s)	882, 1079
BstC8I	GCNNGC	1 cut(s)	642
BstDEI	CTNAG	7 cut(s)	123, 169, 189, 359, 470, 570, 1027
BstF5I	GGATG	3 cut(s)	52, 84, 487
BstFNI	CGCG	1 cut(s)	600
BstHHI	GCGC	1 cut(s)	14
BstKTI	GATC	1 cut(s)	822
BstMAI	GTCTC	2 cut(s)	186, 725
BstMBI	GATC	1 cut(s)	819
BstMWI	GCNNNNNNNGC	2 cut(s)	799, 1037
BstNI	CCWGG	3 cut(s)	234, 386, 756
BstNSI	RCATGY	1 cut(s)	790
BstSCI	CCNGG	3 cut(s)	232, 384, 754
BstSLI	GKGCMC	1 cut(s)	1075
BstSNI	TACGTA	1 cut(s)	882
BstUI	CGCG	1 cut(s)	600
BstV1I	GCAGC	1 cut(s)	1018
BstV2I	GAAGAC	2 cut(s)	44, 1077
Bsu36I	CCTNAGG	1 cut(s)	123
BsuI	GTATCC	1 cut(s)	894
BsuRI	GGCC	5 cut(s)	83, 128, 232, 754, 928
BtsCI	GGATG	3 cut(s)	52, 84, 487
BtsIMutI	CAGTG	2 cut(s)	147, 248
Cac8I	GCNNGC	1 cut(s)	642
CaiI	CAGNNNCTG	1 cut(s)	689
CfoI	GCGC	1 cut(s)	14
Cfr10I	RCCGGY	1 cut(s)	1093
Cfr13I	GGNCC	3 cut(s)	592, 695, 926
CseI	GACGC	1 cut(s)	589
Csp6I	GTAC	1 cut(s)	289
CviAII	CATG	2 cut(s)	611, 787
CviQI	GTAC	1 cut(s)	289
DdeI	CTNAG	7 cut(s)	123, 169, 189, 359, 470, 570, 1027
DpnI	GATC	1 cut(s)	821
DpnII	GATC	1 cut(s)	819
DraIII	CACNNNGTG	1 cut(s)	1079
DrdI	GACNNNNNNGTC	1 cut(s)	599
DseDI	GACNNNNNNGTC	1 cut(s)	599
EaeI	YGGCCR	1 cut(s)	230
Eam1104I	CTCTTC	1 cut(s)	13
EarI	CTCTTC	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	980
Eco105I	TACGTA	1 cut(s)	882
Eco147I	AGGCCT	1 cut(s)	754
Eco24I	GRGCYC	1 cut(s)	982
Eco47I	GGWCC	2 cut(s)	592, 695
Eco53kI	GAGCTC	1 cut(s)	980
Eco57I	CTGAAG	1 cut(s)	669
Eco81I	CCTNAGG	1 cut(s)	123
EcoICRI	GAGCTC	1 cut(s)	980
EcoO109I	RGGNCCY	2 cut(s)	592, 926
EcoRI	GAATTC	1 cut(s)	629
EcoRII	CCWGG	3 cut(s)	232, 384, 754
EcoT38I	GRGCYC	1 cut(s)	982
FaeI	CATG	2 cut(s)	614, 790
FatI	CATG	2 cut(s)	610, 786
FauI	CCCGC	1 cut(s)	916
Fnu4HI	GCNGC	1 cut(s)	1032
FokI	GGATG	3 cut(s)	59, 71, 474
FriOI	GRGCYC	1 cut(s)	982
Fsp4HI	GCNGC	1 cut(s)	1032
FspBI	CTAG	1 cut(s)	741
GlaI	GCGC	1 cut(s)	13
GluI	GCNGC	1 cut(s)	1032
HaeIII	GGCC	5 cut(s)	83, 128, 232, 754, 928
HapII	CCGG	1 cut(s)	1094
HgaI	GACGC	1 cut(s)	589
HhaI	GCGC	1 cut(s)	14
Hin1II	CATG	2 cut(s)	614, 790
Hin6I	GCGC	1 cut(s)	12
HinP1I	GCGC	1 cut(s)	12
HinfI	GANTC	4 cut(s)	173, 311, 677, 1082
HpaII	CCGG	1 cut(s)	1094
HphI	GGTGA	4 cut(s)	248, 467, 629, 1078
Hpy166II	GTNNAC	2 cut(s)	604, 1073
Hpy188I	TCNGA	6 cut(s)	76, 172, 190, 286, 685, 1018
Hpy188III	TCNNGA	4 cut(s)	341, 590, 812, 1054
Hpy8I	GTNNAC	2 cut(s)	604, 1073
HpyAV	CCTTC	8 cut(s)	46, 73, 97, 99, 187, 501, 617, 644
HpyCH4III	ACNGT	2 cut(s)	151, 436
HpyCH4IV	ACGT	2 cut(s)	881, 1078
HpyCH4V	TGCA	4 cut(s)	380, 644, 1024, 1073
HpyF10VI	GCNNNNNNNGC	2 cut(s)	799, 1037
HpyF3I	CTNAG	7 cut(s)	123, 169, 189, 359, 470, 570, 1027
HpySE526I	ACGT	2 cut(s)	881, 1078
Hsp92II	CATG	2 cut(s)	614, 790
HspAI	GCGC	1 cut(s)	12
Kzo9I	GATC	1 cut(s)	819
LmnI	GCTCC	1 cut(s)	798
Lsp1109I	GCAGC	1 cut(s)	1018
LweI	GCATC	4 cut(s)	610, 652, 811, 1011
MaeI	CTAG	1 cut(s)	741
MaeII	ACGT	2 cut(s)	881, 1078
MaeIII	GTNAC	1 cut(s)	59
MalI	GATC	1 cut(s)	821
MboI	GATC	1 cut(s)	819
MboII	GAAGA	3 cut(s)	30, 49, 1077
MhlI	GDGCHC	3 cut(s)	917, 982, 1075
MlsI	TGGCCA	1 cut(s)	232
MluCI	AATT	5 cut(s)	417, 629, 669, 856, 955
MluNI	TGGCCA	1 cut(s)	232
MlyI	GAGTC	2 cut(s)	320, 1091
MmeI	TCCRAC	1 cut(s)	562
Mox20I	TGGCCA	1 cut(s)	232
MroXI	GAANNNNTTC	2 cut(s)	264, 563
MscI	TGGCCA	1 cut(s)	232
MseI	TTAA	3 cut(s)	635, 749, 1002
MslI	CAYNNNNRTG	2 cut(s)	609, 615
Msp20I	TGGCCA	1 cut(s)	232
MspA1I	CMGCKG	3 cut(s)	358, 923, 1031
MspCI	CTTAAG	2 cut(s)	634, 1001
MspI	CCGG	1 cut(s)	1094
MspR9I	CCNGG	3 cut(s)	234, 386, 756
Mva1269I	GAATGC	1 cut(s)	35
MvaI	CCWGG	3 cut(s)	234, 386, 756
MvnI	CGCG	1 cut(s)	600
MwoI	GCNNNNNNNGC	2 cut(s)	799, 1037
NdeII	GATC	1 cut(s)	819
NlaIII	CATG	2 cut(s)	614, 790
NlaIV	GGNNCC	3 cut(s)	594, 794, 927
NmeAIII	GCCGAG	1 cut(s)	154
NspI	RCATGY	1 cut(s)	790
PceI	AGGCCT	1 cut(s)	754
PctI	GAATGC	1 cut(s)	35
PdmI	GAANNNNTTC	2 cut(s)	264, 563
PfeI	GAWTC	2 cut(s)	173, 677
PflFI	GACNNNGTC	1 cut(s)	689
PkrI	GCNGC	1 cut(s)	1033
PleI	GAGTC	2 cut(s)	319, 1090
PpsI	GAGTC	2 cut(s)	319, 1090
Ppu21I	YACGTR	2 cut(s)	882, 1079
PpuMI	RGGWCCY	1 cut(s)	592
Psp124BI	GAGCTC	1 cut(s)	982
Psp5II	RGGWCCY	1 cut(s)	592
Psp6I	CCWGG	3 cut(s)	232, 384, 754
PspGI	CCWGG	3 cut(s)	232, 384, 754
PspN4I	GGNNCC	3 cut(s)	594, 794, 927
PspPI	GGNCC	3 cut(s)	592, 695, 926
PspPPI	RGGWCCY	1 cut(s)	592
PstNI	CAGNNNCTG	1 cut(s)	689
PsyI	GACNNNGTC	1 cut(s)	689
PvuII	CAGCTG	2 cut(s)	358, 1031
RsaI	GTAC	1 cut(s)	290
RsaNI	GTAC	1 cut(s)	289
RseI	CAYNNNNRTG	2 cut(s)	609, 615
SacI	GAGCTC	1 cut(s)	982
SaqAI	TTAA	3 cut(s)	635, 749, 1002
SatI	GCNGC	1 cut(s)	1032
Sau3AI	GATC	1 cut(s)	819
Sau96I	GGNCC	3 cut(s)	592, 695, 926
SchI	GAGTC	2 cut(s)	320, 1091
ScrFI	CCNGG	3 cut(s)	234, 386, 756
SduI	GDGCHC	3 cut(s)	917, 982, 1075
SfaNI	GCATC	4 cut(s)	610, 652, 811, 1011
SinI	GGWCC	2 cut(s)	592, 695
SmiMI	CAYNNNNRTG	2 cut(s)	609, 615
SmlI	CTYRAG	3 cut(s)	634, 1001, 1054
SmoI	CTYRAG	3 cut(s)	634, 1001, 1054
SnaBI	TACGTA	1 cut(s)	882
Sse9I	AATT	5 cut(s)	417, 629, 669, 856, 955
SseBI	AGGCCT	1 cut(s)	754
SsiI	CCGC	1 cut(s)	923
SspI	AATATT	2 cut(s)	265, 840
SspMI	CTAG	1 cut(s)	741
SstI	GAGCTC	1 cut(s)	982
StuI	AGGCCT	1 cut(s)	754
StyD4I	CCNGG	3 cut(s)	232, 384, 754
TaaI	ACNGT	2 cut(s)	151, 436
TaiI	ACGT	2 cut(s)	884, 1081
TaqII	GACCGA	1 cut(s)	683
TasI	AATT	5 cut(s)	417, 629, 669, 856, 955
TfiI	GAWTC	2 cut(s)	173, 677
Tru1I	TTAA	3 cut(s)	635, 749, 1002
Tru9I	TTAA	3 cut(s)	635, 749, 1002
TscAI	CASTG	2 cut(s)	154, 255
TseI	GCWGC	1 cut(s)	1031
TspDTI	ATGAA	2 cut(s)	387, 599
TspRI	CASTG	2 cut(s)	154, 255
Tth111I	GACNNNGTC	1 cut(s)	689
Vha464I	CTTAAG	2 cut(s)	634, 1001
VneI	GTGCAC	1 cut(s)	1071
VpaK11BI	GGWCC	2 cut(s)	592, 695
XapI	RAATTY	1 cut(s)	629
XceI	RCATGY	1 cut(s)	790
XcmI	CCANNNNNNNNNTGG	1 cut(s)	436
XmnI	GAANNNNTTC	2 cut(s)	264, 563
XspI	CTAG	1 cut(s)	741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.