Rorug01G0426900

Belongs to the spermidine spermine synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004702
Physical Location & Seq
Reverse (-)
52687651 .. 52691391
3741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0426900.1

Sequence Viewer

Length: 1539 bp
ATGGTTGGTGTTGTCAAGGACAGATTGGGAACAATGCCTTCTGCCCCTGACTTTGTGCTGTGGCGGACGCCGTTTTATGATGTGAAGAGCTTGAAGGGCATTCCTGATTCCTACAATGACTTTACTAAACTTCGATTGCCAATAACCACGCCGCTTCCGCCAGCTACATTTTCCGGTAGTGCAATGGAGTTGGACTGGGGTCCTATGCCCACATTTGATGATTTGAAGGAGTTTGTAAACGCAAATCCATGCAAAATGCAAGAGAGGTGGACTTCAATCAAGGTCCAGTCAAAGAGTCGTGATGAAGTGTTTGCCACCACAAAAGGAAATTCTATGGGGGGTCAGACGAGCACGGTATTGAATTCTTTGGGTGCATACTTGAGATACTTGGATGGAGAATCACGGGATGACTTCTGGCAGAAGGTGCATGAAAGGATGCCCTATGCTGAAAGCCGGGATGGAGCTTCATTTAATACTCTCTTTGGTACAGCCCTTTCTCTTGGCATCCTATCCAGAAGGAGAGTATATTTTGAAGCTATTAAGCATGACAAAGAACGAAACTGTGGATTTCTATCTCCTTTTGGTTCAGGAGCAACAATTTCTGCTGCTGTTGATTCAGTGTGCTCAATGGAGTGGTATTGGCTTCTGGCTCTCAAAAGTCAGATAAGTAATGATAGAAGATATCATATTCGTATCTGGAGATGGAAAGGCTATCTAATTCAGTATACTGTTGTTGGTCATGAAGGTCCTGCTATTCTGCTTGTGCATGGTTTTGGAGCCTTTTTGGAGCATTACCGTGATAACCTAGGTCGCATCGCTGAAAGTGGAAACCGAGTTTGGGCCATTACAATTTTAGGATTTGGGAAATCAGAGAAGCCAAATATTGTGTACACTGAACTCCTGTGGTCTGAAATGCTTAGAGATTTCATTCTTGAAGTTGTGGGTGAACCAGTGCATCTTGTTGGGAACTCAATTGGTGGTTATATTATTGCAATTGTTGCTCGTCTTTGGCCTGCTCTGGCCAAATCTGTTGTTCTGATTAACAGTGGGGGTGAGGTTATTCCATACTCCTCCCCGCCATTCACTAAAGAAAGAAAAACATCAGGGGCTTCATGGCTGGGTGCTCGATTTCTTTTGTTCTACCTAAGGTTTCAACTCAAGGACATAGTGCAGAATTGCTATCCAAATAAAACAGAGCGAGTGGACAATTGGCTTATAAATGAAATGCTAAGAGCATCATATGATCCTGGGGTGGCAGTGGTCCTAGAAAGCGTTTTTAGCTTCAATCTCTCACTTCCTCTTAATTATCTTTTGAAAGGATTCAAGGAAAAGGTTCTCATTATCCAGGGAATGAGGGATCCAATATCCAACTCCAAATCAAAAGTGGCTATGATTAAAGAGCATTCCGATGGGTTCTTAATCAAGGAATTGGATGCTGGTCATTGTCCTCACGACGAACTGCCTGAGGAAGTAAATTCAATTATCTGTGAATGGATAGTAACCCTGACTAGTAAACGCCCTGTTGTGAGCTTTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100
Pfam Domains
Protein Families
Spermine_synt_N Spermine_synth

Protein Analysis

512

Amino Acids

57.86

Weight (kDa)

8.53

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 250 - 486 5.1e-14 alpha/beta hydrolase fold
Hydrolase_4 PF12146 251 - 369 1.2e-07 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 252 - 492 1.7e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1217
AccI GTMKAC 1 cut(s) 725
AciI CCGC 4 cut(s) 64, 152, 158, 1076
AclWI GGATC 3 cut(s) 1238, 1352, 1365
AcoI YGGCCR 1 cut(s) 1020
AcsI RAATTY 3 cut(s) 328, 361, 1474
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 2 cut(s) 487, 890
AfiI CCNNNNNNNGG 1 cut(s) 838
AhlI ACTAGT 1 cut(s) 1508
AjnI CCWGG 2 cut(s) 1246, 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 820, 852
AluBI AGCT 6 cut(s) 90, 164, 464, 536, 1281, 1530
AluI AGCT 6 cut(s) 90, 164, 464, 536, 1281, 1530
Alw21I GWGCWC 3 cut(s) 353, 626, 1126
AlwI GGATC 3 cut(s) 1238, 1352, 1365
AoxI GGCC 3 cut(s) 840, 1010, 1020
ApeKI GCWGC 1 cut(s) 605
ApoI RAATTY 3 cut(s) 328, 361, 1474
Asp700I GAANNNNTTC 2 cut(s) 1319, 1332
AspA2I CCTAGG 1 cut(s) 805
AspS9I GGNCC 5 cut(s) 200, 283, 746, 840, 1261
AsuC2I CCSGG 1 cut(s) 455
AsuHPI GGTGA 2 cut(s) 956, 1064
AvaII GGWCC 4 cut(s) 200, 283, 746, 1261
AvrII CCTAGG 1 cut(s) 805
AxyI CCTNAGG 2 cut(s) 1145, 1464
BalI TGGCCA 1 cut(s) 1022
BamHI GGATCC 1 cut(s) 1357
Bbv12I GWGCWC 3 cut(s) 353, 626, 1126
BbvI GCAGC 1 cut(s) 592
BccI CCATC 4 cut(s) 386, 452, 696, 1403
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 2 cut(s) 1248, 1346
BcnI CCSGG 1 cut(s) 455
BcuI ACTAGT 1 cut(s) 1508
BfaI CTAG 3 cut(s) 806, 1265, 1509
BisI GCNGC 2 cut(s) 152, 606
BlnI CCTAGG 1 cut(s) 805
BlsI GCNGC 2 cut(s) 153, 607
Bme1390I CCNGG 3 cut(s) 455, 1248, 1346
Bme18I GGWCC 4 cut(s) 200, 283, 746, 1261
BmgT120I GGNCC 5 cut(s) 200, 283, 746, 840, 1261
BmiI GGNNCC 3 cut(s) 201, 778, 1359
BmrFI CCNGG 3 cut(s) 455, 1248, 1346
BmrI ACTGGG 1 cut(s) 205
BmsI GCATC 6 cut(s) 426, 513, 822, 964, 1244, 1423
BmuI ACTGGG 1 cut(s) 205
BoxI GACNNNNGTC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 718
BpuEI CTTGAG 2 cut(s) 400, 1142
BpuMI CCSGG 1 cut(s) 455
BsaBI GATNNNNATC 1 cut(s) 571
BsaHI GRCGYC 1 cut(s) 68
BsaJI CCNNGG 3 cut(s) 805, 1247, 1345
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 1 cut(s) 838
Bse1I ACTGG 3 cut(s) 200, 286, 950
Bse21I CCTNAGG 2 cut(s) 1145, 1464
Bse3DI GCAATG 1 cut(s) 189
Bse8I GATNNNNATC 1 cut(s) 571
BseBI CCWGG 2 cut(s) 1248, 1346
BseDI CCNNGG 3 cut(s) 805, 1247, 1345
BseGI GGATG 6 cut(s) 397, 412, 441, 463, 504, 1438
BseJI GATNNNNATC 1 cut(s) 571
BseLI CCNNNNNNNGG 1 cut(s) 838
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 1455
BseNI ACTGG 3 cut(s) 200, 286, 950
BseRI GAGGAG 1 cut(s) 1060
BseXI GCAGC 1 cut(s) 592
BseYI CCCAGC 1 cut(s) 1117
BsgI GTGCAG 1 cut(s) 1190
BshFI GGCC 3 cut(s) 842, 1012, 1022
BsiHKAI GWGCWC 3 cut(s) 353, 626, 1126
BsiSI CCGG 2 cut(s) 174, 454
BslI CCNNNNNNNGG 1 cut(s) 838
BsmI GAATGC 2 cut(s) 99, 1402
BsnI GGCC 3 cut(s) 842, 1012, 1022
Bsp1286I GDGCHC 3 cut(s) 353, 626, 1126
Bsp1407I TGTACA 1 cut(s) 888
Bsp143I GATC 2 cut(s) 1243, 1357
BspACI CCGC 4 cut(s) 64, 152, 158, 1076
BspANI GGCC 3 cut(s) 842, 1012, 1022
BspCNI CTCAG 1 cut(s) 1456
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 3 cut(s) 201, 778, 1359
BspPI GGATC 3 cut(s) 1238, 1352, 1365
BspQI GCTCTTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 189
BsrGI TGTACA 1 cut(s) 888
BsrI ACTGG 3 cut(s) 200, 286, 950
BssECI CCNNGG 3 cut(s) 805, 1247, 1345
BssMI GATC 2 cut(s) 1243, 1357
BssNAI GTATAC 1 cut(s) 726
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 805
Bst1107I GTATAC 1 cut(s) 726
Bst2UI CCWGG 2 cut(s) 1248, 1346
Bst4CI ACNGT 5 cut(s) 355, 563, 730, 797, 1046
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 1 cut(s) 68
BstAPI GCANNNNNTGC 2 cut(s) 424, 998
BstAUI TGTACA 1 cut(s) 888
BstC8I GCNNGC 2 cut(s) 162, 1014
BstDEI CTNAG 4 cut(s) 917, 1145, 1229, 1464
BstF5I GGATG 6 cut(s) 397, 412, 441, 463, 504, 1438
BstKTI GATC 2 cut(s) 1246, 1360
BstMBI GATC 2 cut(s) 1243, 1357
BstMWI GCNNNNNNNGC 5 cut(s) 96, 157, 424, 998, 1278
BstNI CCWGG 2 cut(s) 1248, 1346
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 3 cut(s) 453, 1246, 1344
BstV1I GCAGC 1 cut(s) 592
BstX2I RGATCY 1 cut(s) 1357
BstYI RGATCY 1 cut(s) 1357
BstZ17I GTATAC 1 cut(s) 726
Bsu36I CCTNAGG 2 cut(s) 1145, 1464
BsuRI GGCC 3 cut(s) 842, 1012, 1022
BtgZI GCGATG 1 cut(s) 799
BtsCI GGATG 6 cut(s) 397, 412, 441, 463, 504, 1438
BtsI GCAGTG 1 cut(s) 1263
BtsIMutI CAGTG 5 cut(s) 624, 891, 957, 1051, 1263
Cac8I GCNNGC 2 cut(s) 162, 1014
CciI TCATGA 1 cut(s) 739
Cfr13I GGNCC 5 cut(s) 200, 283, 746, 840, 1261
CseI GACGC 1 cut(s) 76
Csp6I GTAC 2 cut(s) 486, 889
CspCI CAANNNNNGTGG 2 cut(s) 248, 283
CviAII CATG 6 cut(s) 249, 428, 545, 740, 767, 1113
CviQI GTAC 2 cut(s) 486, 889
DdeI CTNAG 4 cut(s) 917, 1145, 1229, 1464
DpnI GATC 2 cut(s) 1245, 1359
DpnII GATC 2 cut(s) 1243, 1357
EaeI YGGCCR 1 cut(s) 1020
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 2 cut(s) 79, 147
Eco130I CCWWGG 1 cut(s) 805
Eco32I GATATC 1 cut(s) 683
Eco47I GGWCC 4 cut(s) 200, 283, 746, 1261
Eco81I CCTNAGG 2 cut(s) 1145, 1464
EcoO109I RGGNCCY 2 cut(s) 200, 746
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 2 cut(s) 1246, 1344
EcoRV GATATC 1 cut(s) 683
EcoT14I CCWWGG 1 cut(s) 805
ErhI CCWWGG 1 cut(s) 805
FaeI CATG 6 cut(s) 252, 431, 548, 743, 770, 1116
FatI CATG 6 cut(s) 248, 427, 544, 739, 766, 1112
FauI CCCGC 1 cut(s) 1083
FauNDI CATATG 1 cut(s) 1240
FblI GTMKAC 1 cut(s) 725
Fnu4HI GCNGC 2 cut(s) 152, 606
FokI GGATG 6 cut(s) 404, 419, 448, 470, 491, 1445
Fsp4HI GCNGC 2 cut(s) 152, 606
FspBI CTAG 3 cut(s) 806, 1265, 1509
GluI GCNGC 2 cut(s) 152, 606
GsaI CCCAGC 1 cut(s) 1121
GsuI CTGGAG 1 cut(s) 718
HaeIII GGCC 3 cut(s) 842, 1012, 1022
HapII CCGG 2 cut(s) 174, 454
HgaI GACGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 6 cut(s) 252, 431, 548, 743, 770, 1116
HinfI GANTC 5 cut(s) 107, 295, 398, 614, 1320
HpaII CCGG 2 cut(s) 174, 454
HphI GGTGA 2 cut(s) 956, 1064
Hpy166II GTNNAC 8 cut(s) 238, 270, 726, 889, 891, 947, 1204, 1514
Hpy188I TCNGA 6 cut(s) 345, 663, 871, 910, 1038, 1408
Hpy188III TCNNGA 8 cut(s) 104, 299, 513, 588, 697, 740, 932, 1451
Hpy8I GTNNAC 8 cut(s) 238, 270, 726, 889, 891, 947, 1204, 1514
Hpy99I CGWCG 1 cut(s) 1457
HpyAV CCTTC 6 cut(s) 48, 88, 220, 415, 510, 737
HpyCH4III ACNGT 5 cut(s) 355, 563, 730, 797, 1046
HpyCH4V TGCA 9 cut(s) 182, 252, 259, 374, 427, 766, 955, 992, 1171
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 157, 424, 998, 1278
HpyF3I CTNAG 4 cut(s) 917, 1145, 1229, 1464
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 6 cut(s) 252, 431, 548, 743, 770, 1116
Kzo9I GATC 2 cut(s) 1243, 1357
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 4 cut(s) 461, 590, 776, 787
Lsp1109I GCAGC 1 cut(s) 592
LweI GCATC 6 cut(s) 426, 513, 822, 964, 1244, 1423
MaeI CTAG 3 cut(s) 806, 1265, 1509
MaeIII GTNAC 1 cut(s) 1498
MalI GATC 2 cut(s) 1245, 1359
MboI GATC 2 cut(s) 1243, 1357
MboII GAAGA 2 cut(s) 97, 690
MfeI CAATTG 3 cut(s) 972, 993, 1207
MflI RGATCY 1 cut(s) 1357
MhlI GDGCHC 3 cut(s) 353, 626, 1126
MlsI TGGCCA 1 cut(s) 1022
MluNI TGGCCA 1 cut(s) 1022
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 2 cut(s) 171, 1392
MnlI CCTC 7 cut(s) 258, 1048, 1081, 1308, 1347, 1458, 1459
Mox20I TGGCCA 1 cut(s) 1022
MroXI GAANNNNTTC 2 cut(s) 1319, 1332
MscI TGGCCA 1 cut(s) 1022
MseI TTAA 6 cut(s) 471, 540, 1041, 1302, 1395, 1418
MslI CAYNNNNRTG 2 cut(s) 795, 1407
Msp20I TGGCCA 1 cut(s) 1022
MspI CCGG 2 cut(s) 174, 454
MspR9I CCNGG 3 cut(s) 455, 1248, 1346
MunI CAATTG 3 cut(s) 972, 993, 1207
Mva1269I GAATGC 2 cut(s) 99, 1402
MvaI CCWGG 2 cut(s) 1248, 1346
MwoI GCNNNNNNNGC 5 cut(s) 96, 157, 424, 998, 1278
NciI CCSGG 1 cut(s) 455
NdeI CATATG 1 cut(s) 1240
NdeII GATC 2 cut(s) 1243, 1357
NlaIII CATG 6 cut(s) 252, 431, 548, 743, 770, 1116
NlaIV GGNNCC 3 cut(s) 201, 778, 1359
PagI TCATGA 1 cut(s) 739
PciSI GCTCTTC 1 cut(s) 80
PctI GAATGC 2 cut(s) 99, 1402
PdmI GAANNNNTTC 2 cut(s) 1319, 1332
PfeI GAWTC 4 cut(s) 107, 398, 614, 1320
PkrI GCNGC 2 cut(s) 153, 607
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PpuMI RGGWCCY 2 cut(s) 200, 746
PshAI GACNNNNGTC 1 cut(s) 198
PsiI TTATAA 1 cut(s) 1217
Psp5II RGGWCCY 2 cut(s) 200, 746
Psp6I CCWGG 2 cut(s) 1246, 1344
PspFI CCCAGC 1 cut(s) 1117
PspGI CCWGG 2 cut(s) 1246, 1344
PspN4I GGNNCC 3 cut(s) 201, 778, 1359
PspPI GGNCC 5 cut(s) 200, 283, 746, 840, 1261
PspPPI RGGWCCY 2 cut(s) 200, 746
PsuI RGATCY 1 cut(s) 1357
RsaI GTAC 2 cut(s) 487, 890
RsaNI GTAC 2 cut(s) 486, 889
RseI CAYNNNNRTG 2 cut(s) 795, 1407
SapI GCTCTTC 1 cut(s) 80
SaqAI TTAA 6 cut(s) 471, 540, 1041, 1302, 1395, 1418
SatI GCNGC 2 cut(s) 152, 606
Sau3AI GATC 2 cut(s) 1243, 1357
Sau96I GGNCC 5 cut(s) 200, 283, 746, 840, 1261
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 3 cut(s) 455, 1248, 1346
SduI GDGCHC 3 cut(s) 353, 626, 1126
SfaNI GCATC 6 cut(s) 426, 513, 822, 964, 1244, 1423
SinI GGWCC 4 cut(s) 200, 283, 746, 1261
SmiMI CAYNNNNRTG 2 cut(s) 795, 1407
SmlI CTYRAG 2 cut(s) 379, 1157
SmoI CTYRAG 2 cut(s) 379, 1157
SpeI ACTAGT 1 cut(s) 1508
SsiI CCGC 4 cut(s) 64, 152, 158, 1076
SspI AATATT 1 cut(s) 883
SspMI CTAG 3 cut(s) 806, 1265, 1509
StyD4I CCNGG 3 cut(s) 453, 1246, 1344
StyI CCWWGG 1 cut(s) 805
TaaI ACNGT 5 cut(s) 355, 563, 730, 797, 1046
TaqI TCGA 2 cut(s) 133, 1126
TatI WGTACW 1 cut(s) 888
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 4 cut(s) 107, 398, 614, 1320
Tru1I TTAA 6 cut(s) 471, 540, 1041, 1302, 1395, 1418
Tru9I TTAA 6 cut(s) 471, 540, 1041, 1302, 1395, 1418
TscAI CASTG 5 cut(s) 624, 898, 957, 1051, 1263
TseI GCWGC 1 cut(s) 605
TspDTI ATGAA 7 cut(s) 318, 444, 456, 756, 916, 1101, 1236
TspRI CASTG 5 cut(s) 624, 898, 957, 1051, 1263
VpaK11BI GGWCC 4 cut(s) 200, 283, 746, 1261
XapI RAATTY 3 cut(s) 328, 361, 1474
XcmI CCANNNNNNNNNTGG 1 cut(s) 1381
XmaJI CCTAGG 1 cut(s) 805
XmiI GTMKAC 1 cut(s) 725
XmnI GAANNNNTTC 2 cut(s) 1319, 1332
XspI CTAG 3 cut(s) 806, 1265, 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.