Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004702

Physical Location & Seq

Genomic Coordinates Forward (+)

35184345 .. 35189215

Gene Structure 4871 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1291100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGAGGGAGGCGCAGGAAGAGGTTTGGAATGCCAGAAGACTATGGATGGGAAGGCGAGTAACGGGAATGGTTCGGAGAAGGCCATCCCTTCTTGTTGCTTGAAGGCTAGGGCTTCTATCCCTGAGCCGGAGGCAAAATGTCATCCTACTGTTGTTTCTGGGTGGTTCTCAGAATCCCAGTCTCGCTCTGAGAAGGCTTGTAAAAAGCTTTATTTCAACAACCCGATGTGGCCAGGTGAAGCTCGTTCACTGAAAGTAGAAAATATTCTGTATAGGGGGAAATCAGAGTTCCAAGAGATTTTGGTGTTTGAGTCCTCAATATATGGAAAAGTGCTTGTTCTTGATGGCCTTGTCCAGCTGAGTGAGAAAGATGAATGTGCATACCAGGAGATGATAGCACATCTACCTCTTTGTTCAATTCCCGCCCCCAAGACAGTTCTGGTGGTTGGCGGTGGTGATGGTGGGGTTCTTAGGGAGGTTTCTCGCCATCCTTCTGTTGAGCATATTGATATATGTGAGATAGATAAGATGGTTATTGATGTGTCCAAGAAGTTTTTTCCTCGGTTGGCTGTTGGATTTGAGGACCCTCGCGTGCACCTTCACATCGCTGATGCTACTGAATTCTTAAGGCTTGCACCTGAAGGGAAGTATGATGCTGTAATTGTTGATTCGTCAGACCCTGTTGGTCCCGCCCAAGAGCTTGTAGAGAAGCCATTTTTTGAGACAATAGCTAGAGCATTAAGGCCTGGTGGTGTTCTCTGTAACATGGCAGAGAGCATGTGGCTCCATACACATCTTATTCAAGATCTGATCTCTGTTTGCCATCAAACATTCAAGGGGTCTGTCGAATATGCTTGGGCGAGTGTTCCTACGTATCCAAGTGGTGTGATAGGTTTTCTGTTGTGCTCAACAGAGGGGCCTCCCGTTGATTTCAAAAACCCCGTCAATTCTATTGAGAAGTTAGAAGGAGCTCTCAAACATACGAGAGAACTCCAGTTCTATAACTCCGAGATGCACTCAGCTGCCTTTGCGTTGCCTTCTTTCTTGAGAAGGGAGGTGAGTGCGCTACGTGAATCTTCAACTCCGGCAAGACAAATCGGGGAGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

370

Amino Acids

40.58

Weight (kDa)

5.83

Isoelectric Point (pI)

53.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	75 - 122	8.1e-18	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	125 - 313	2.8e-70	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004702)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53120 AT5G53120 AT5G53120 AT5G53120 AT5G53120 AT5G53120 AT5G53120
fragaria_vesca	FvH4_7g31820 FvH4_7g31820
malus_domestica	MD01G1221000.v1.1 MD07G1291100.v1.1
prunus_persica	Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1 Prupe.2G312400_v2.0.a1
pyrus_communis	pycom01g23040 pycom07g26370
rosa_chinensis	RchiOBHm_Chr1g0381051
rosa_laevigata	RLG00000026249
rosa_multiflora	Rmu_sc0004494.1_g000004
rosa_roxburghii	Rroxscaffold_4G00278820
rosa_rugosa	Rorug01G0426900
rosa_samantha	Rh1CG420800
rosa_wichuraiana	Rw1G039270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	591
AciI	CCGC	3 cut(s)	423, 450, 690
AcoI	YGGCCR	1 cut(s)	230
AcsI	RAATTY	1 cut(s)	620
AcuI	CTGAAG	1 cut(s)	660
AfiI	CCNNNNNNNGG	1 cut(s)	127
AflII	CTTAAG	1 cut(s)	625
AgsI	TTSAA	7 cut(s)	103, 217, 417, 803, 835, 934, 1080
AjnI	CCWGG	3 cut(s)	232, 384, 745
AluBI	AGCT	7 cut(s)	208, 242, 358, 700, 731, 971, 1022
AluI	AGCT	7 cut(s)	208, 242, 358, 700, 731, 971, 1022
Alw21I	GWGCWC	3 cut(s)	597, 908, 973
Alw26I	GTCTC	2 cut(s)	186, 716
Alw44I	GTGCAC	1 cut(s)	593
AlwNI	CAGNNNCTG	1 cut(s)	680
AoxI	GGCC	5 cut(s)	81, 230, 346, 743, 917
ApaLI	GTGCAC	1 cut(s)	593
ApeKI	GCWGC	1 cut(s)	1022
ApoI	RAATTY	1 cut(s)	620
ArsI	GACNNNNNNTTYG	2 cut(s)	927, 959
Asp700I	GAANNNNTTC	1 cut(s)	264
AspLEI	GCGC	2 cut(s)	14, 1066
AspS9I	GGNCC	3 cut(s)	583, 686, 917
AsuHPI	GGTGA	3 cut(s)	248, 467, 1069
AvaII	GGWCC	2 cut(s)	583, 686
BaeGI	GKGCMC	1 cut(s)	597
BalI	TGGCCA	1 cut(s)	232
BanII	GRGCYC	1 cut(s)	973
BbsI	GAAGAC	1 cut(s)	44
Bbv12I	GWGCWC	3 cut(s)	597, 908, 973
BbvI	GCAGC	1 cut(s)	1009
BccI	CCATC	7 cut(s)	41, 92, 338, 452, 495, 523, 831
BciT130I	CCWGG	3 cut(s)	234, 386, 747
BciVI	GTATCC	1 cut(s)	885
BcoDI	GTCTC	2 cut(s)	186, 716
BfaI	CTAG	2 cut(s)	108, 732
BfrI	CTTAAG	1 cut(s)	625
BfuI	GTATCC	1 cut(s)	885
BglII	AGATCT	1 cut(s)	805
BisI	GCNGC	1 cut(s)	1023
BlsI	GCNGC	1 cut(s)	1024
Bme1390I	CCNGG	3 cut(s)	234, 386, 747
Bme18I	GGWCC	2 cut(s)	583, 686
BmgT120I	GGNCC	3 cut(s)	583, 686, 917
BmiI	GGNNCC	4 cut(s)	585, 688, 785, 918
BmrFI	CCNGG	3 cut(s)	234, 386, 747
BmrI	ACTGGG	1 cut(s)	172
BmsI	GCATC	3 cut(s)	601, 643, 1002
BmuI	ACTGGG	1 cut(s)	172
BpiI	GAAGAC	1 cut(s)	44
BplI	GAGNNNNNCTC	2 cut(s)	1001, 1033
BpmI	CTGGAG	1 cut(s)	977
Bpu10I	CCTNAGC	1 cut(s)	123
BpuEI	CTTGAG	1 cut(s)	1066
BsaAI	YACGTR	2 cut(s)	873, 1070
BsaJI	CCNNGG	1 cut(s)	560
Bsc4I	CCNNNNNNNGG	1 cut(s)	127
Bse1I	ACTGG	2 cut(s)	178, 994
BseBI	CCWGG	3 cut(s)	234, 386, 747
BseDI	CCNNGG	1 cut(s)	560
BseGI	GGATG	4 cut(s)	52, 84, 142, 487
BseLI	CCNNNNNNNGG	1 cut(s)	127
BseMII	CTCAG	5 cut(s)	114, 180, 183, 350, 1032
BseNI	ACTGG	2 cut(s)	178, 994
BseSI	GKGCMC	1 cut(s)	597
BseXI	GCAGC	1 cut(s)	1009
Bsh1236I	CGCG	1 cut(s)	591
BshFI	GGCC	5 cut(s)	83, 232, 348, 745, 919
BsiHKAI	GWGCWC	3 cut(s)	597, 908, 973
BsiSI	CCGG	2 cut(s)	128, 1085
BslFI	GGGAC	1 cut(s)	672
BslI	CCNNNNNNNGG	1 cut(s)	127
BsmAI	GTCTC	2 cut(s)	186, 716
BsmFI	GGGAC	1 cut(s)	672
BsmI	GAATGC	1 cut(s)	35
BsnI	GGCC	5 cut(s)	83, 232, 348, 745, 919
Bsp1286I	GDGCHC	3 cut(s)	597, 908, 973
Bsp143I	GATC	2 cut(s)	805, 810
BspACI	CCGC	3 cut(s)	423, 450, 690
BspANI	GGCC	5 cut(s)	83, 232, 348, 745, 919
BspCNI	CTCAG	5 cut(s)	115, 181, 182, 351, 1031
BspFNI	CGCG	1 cut(s)	591
BspLI	GGNNCC	4 cut(s)	585, 688, 785, 918
BspTI	CTTAAG	1 cut(s)	625
BsrI	ACTGG	2 cut(s)	178, 994
BssECI	CCNNGG	1 cut(s)	560
BssMI	GATC	2 cut(s)	805, 810
Bst2UI	CCWGG	3 cut(s)	234, 386, 747
Bst4CI	ACNGT	2 cut(s)	151, 436
Bst6I	CTCTTC	1 cut(s)	13
BstAFI	CTTAAG	1 cut(s)	625
BstBAI	YACGTR	2 cut(s)	873, 1070
BstC8I	GCNNGC	2 cut(s)	593, 633
BstDEI	CTNAG	6 cut(s)	123, 169, 189, 359, 470, 1018
BstF5I	GGATG	4 cut(s)	52, 84, 142, 487
BstFNI	CGCG	1 cut(s)	591
BstHHI	GCGC	2 cut(s)	14, 1066
BstKTI	GATC	2 cut(s)	808, 813
BstMAI	GTCTC	2 cut(s)	186, 716
BstMBI	GATC	2 cut(s)	805, 810
BstMWI	GCNNNNNNNGC	1 cut(s)	1028
BstNI	CCWGG	3 cut(s)	234, 386, 747
BstNSI	RCATGY	1 cut(s)	781
BstSCI	CCNGG	3 cut(s)	232, 384, 745
BstSLI	GKGCMC	1 cut(s)	597
BstSNI	TACGTA	1 cut(s)	873
BstUI	CGCG	1 cut(s)	591
BstV1I	GCAGC	1 cut(s)	1009
BstV2I	GAAGAC	1 cut(s)	44
BstX2I	RGATCY	1 cut(s)	805
BstYI	RGATCY	1 cut(s)	805
BsuI	GTATCC	1 cut(s)	885
BsuRI	GGCC	5 cut(s)	83, 232, 348, 745, 919
BtgZI	GCGATG	1 cut(s)	589
BtsCI	GGATG	4 cut(s)	52, 84, 142, 487
BtsIMutI	CAGTG	1 cut(s)	248
Cac8I	GCNNGC	2 cut(s)	593, 633
CaiI	CAGNNNCTG	1 cut(s)	680
CfoI	GCGC	2 cut(s)	14, 1066
Cfr13I	GGNCC	3 cut(s)	583, 686, 917
CviAII	CATG	2 cut(s)	766, 778
DdeI	CTNAG	6 cut(s)	123, 169, 189, 359, 470, 1018
DpnI	GATC	2 cut(s)	807, 812
DpnII	GATC	2 cut(s)	805, 810
EaeI	YGGCCR	1 cut(s)	230
Eam1104I	CTCTTC	1 cut(s)	13
EarI	CTCTTC	1 cut(s)	13
Ecl136II	GAGCTC	1 cut(s)	971
Eco105I	TACGTA	1 cut(s)	873
Eco147I	AGGCCT	1 cut(s)	745
Eco24I	GRGCYC	1 cut(s)	973
Eco47I	GGWCC	2 cut(s)	583, 686
Eco53kI	GAGCTC	1 cut(s)	971
Eco57I	CTGAAG	1 cut(s)	660
EcoICRI	GAGCTC	1 cut(s)	971
EcoO109I	RGGNCCY	2 cut(s)	583, 917
EcoRI	GAATTC	1 cut(s)	620
EcoRII	CCWGG	3 cut(s)	232, 384, 745
EcoT38I	GRGCYC	1 cut(s)	973
FaeI	CATG	2 cut(s)	769, 781
FaqI	GGGAC	1 cut(s)	672
FatI	CATG	2 cut(s)	765, 777
FauI	CCCGC	2 cut(s)	430, 697
Fnu4HI	GCNGC	1 cut(s)	1023
FokI	GGATG	4 cut(s)	59, 71, 129, 474
FriOI	GRGCYC	1 cut(s)	973
Fsp4HI	GCNGC	1 cut(s)	1023
FspBI	CTAG	2 cut(s)	108, 732
GlaI	GCGC	2 cut(s)	13, 1065
GluI	GCNGC	1 cut(s)	1023
GsuI	CTGGAG	1 cut(s)	977
HaeIII	GGCC	5 cut(s)	83, 232, 348, 745, 919
HapII	CCGG	2 cut(s)	128, 1085
HhaI	GCGC	2 cut(s)	14, 1066
Hin1II	CATG	2 cut(s)	769, 781
Hin6I	GCGC	2 cut(s)	12, 1064
HinP1I	GCGC	2 cut(s)	12, 1064
HindIII	AAGCTT	1 cut(s)	206
HinfI	GANTC	4 cut(s)	173, 311, 668, 1073
HpaII	CCGG	2 cut(s)	128, 1085
HphI	GGTGA	3 cut(s)	248, 467, 1069
Hpy166II	GTNNAC	2 cut(s)	248, 595
Hpy188I	TCNGA	7 cut(s)	76, 172, 190, 286, 676, 810, 1009
Hpy188III	TCNNGA	3 cut(s)	341, 803, 1045
Hpy8I	GTNNAC	2 cut(s)	248, 595
HpyCH4III	ACNGT	2 cut(s)	151, 436
HpyCH4IV	ACGT	2 cut(s)	872, 1069
HpyCH4V	TGCA	4 cut(s)	380, 595, 635, 1015
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1028
HpyF3I	CTNAG	6 cut(s)	123, 169, 189, 359, 470, 1018
HpySE526I	ACGT	2 cut(s)	872, 1069
Hsp92II	CATG	2 cut(s)	769, 781
HspAI	GCGC	2 cut(s)	12, 1064
Kzo9I	GATC	2 cut(s)	805, 810
LmnI	GCTCC	2 cut(s)	789, 968
Lsp1109I	GCAGC	1 cut(s)	1009
LweI	GCATC	3 cut(s)	601, 643, 1002
MaeI	CTAG	2 cut(s)	108, 732
MaeII	ACGT	2 cut(s)	872, 1069
MaeIII	GTNAC	2 cut(s)	59, 761
MalI	GATC	2 cut(s)	807, 812
MboI	GATC	2 cut(s)	805, 810
MboII	GAAGA	3 cut(s)	30, 49, 1068
MflI	RGATCY	1 cut(s)	805
MhlI	GDGCHC	3 cut(s)	597, 908, 973
MlsI	TGGCCA	1 cut(s)	232
MluCI	AATT	4 cut(s)	417, 620, 660, 946
MluNI	TGGCCA	1 cut(s)	232
MlyI	GAGTC	1 cut(s)	320
MmeI	TCCRAC	1 cut(s)	553
Mox20I	TGGCCA	1 cut(s)	232
MroXI	GAANNNNTTC	1 cut(s)	264
MscI	TGGCCA	1 cut(s)	232
MseI	TTAA	2 cut(s)	626, 740
Msp20I	TGGCCA	1 cut(s)	232
MspA1I	CMGCKG	2 cut(s)	358, 1022
MspCI	CTTAAG	1 cut(s)	625
MspI	CCGG	2 cut(s)	128, 1085
MspR9I	CCNGG	3 cut(s)	234, 386, 747
Mva1269I	GAATGC	1 cut(s)	35
MvaI	CCWGG	3 cut(s)	234, 386, 747
MvnI	CGCG	1 cut(s)	591
MwoI	GCNNNNNNNGC	1 cut(s)	1028
NdeII	GATC	2 cut(s)	805, 810
NlaIII	CATG	2 cut(s)	769, 781
NlaIV	GGNNCC	4 cut(s)	585, 688, 785, 918
NspI	RCATGY	1 cut(s)	781
PceI	AGGCCT	1 cut(s)	745
PctI	GAATGC	1 cut(s)	35
PdmI	GAANNNNTTC	1 cut(s)	264
PfeI	GAWTC	3 cut(s)	173, 668, 1073
PkrI	GCNGC	1 cut(s)	1024
PleI	GAGTC	1 cut(s)	319
PpsI	GAGTC	1 cut(s)	319
Ppu21I	YACGTR	2 cut(s)	873, 1070
PpuMI	RGGWCCY	1 cut(s)	583
Psp124BI	GAGCTC	1 cut(s)	973
Psp5II	RGGWCCY	1 cut(s)	583
Psp6I	CCWGG	3 cut(s)	232, 384, 745
PspGI	CCWGG	3 cut(s)	232, 384, 745
PspN4I	GGNNCC	4 cut(s)	585, 688, 785, 918
PspPI	GGNCC	3 cut(s)	583, 686, 917
PspPPI	RGGWCCY	1 cut(s)	583
PstNI	CAGNNNCTG	1 cut(s)	680
PsuI	RGATCY	1 cut(s)	805
PvuII	CAGCTG	2 cut(s)	358, 1022
SacI	GAGCTC	1 cut(s)	973
SaqAI	TTAA	2 cut(s)	626, 740
SatI	GCNGC	1 cut(s)	1023
Sau3AI	GATC	2 cut(s)	805, 810
Sau96I	GGNCC	3 cut(s)	583, 686, 917
SchI	GAGTC	1 cut(s)	320
ScrFI	CCNGG	3 cut(s)	234, 386, 747
SduI	GDGCHC	3 cut(s)	597, 908, 973
SfaNI	GCATC	3 cut(s)	601, 643, 1002
SinI	GGWCC	2 cut(s)	583, 686
SmlI	CTYRAG	2 cut(s)	625, 1045
SmoI	CTYRAG	2 cut(s)	625, 1045
SnaBI	TACGTA	1 cut(s)	873
Sse9I	AATT	4 cut(s)	417, 620, 660, 946
SseBI	AGGCCT	1 cut(s)	745
SsiI	CCGC	3 cut(s)	423, 450, 690
SspI	AATATT	1 cut(s)	265
SspMI	CTAG	2 cut(s)	108, 732
SstI	GAGCTC	1 cut(s)	973
StuI	AGGCCT	1 cut(s)	745
StyD4I	CCNGG	3 cut(s)	232, 384, 745
TaaI	ACNGT	2 cut(s)	151, 436
TaiI	ACGT	2 cut(s)	875, 1072
TaqI	TCGA	1 cut(s)	846
TasI	AATT	4 cut(s)	417, 620, 660, 946
TfiI	GAWTC	3 cut(s)	173, 668, 1073
Tru1I	TTAA	2 cut(s)	626, 740
Tru9I	TTAA	2 cut(s)	626, 740
TscAI	CASTG	1 cut(s)	255
TseI	GCWGC	1 cut(s)	1022
TspDTI	ATGAA	1 cut(s)	387
TspRI	CASTG	1 cut(s)	255
Vha464I	CTTAAG	1 cut(s)	625
VneI	GTGCAC	1 cut(s)	593
VpaK11BI	GGWCC	2 cut(s)	583, 686
XapI	RAATTY	1 cut(s)	620
XceI	RCATGY	1 cut(s)	781
XcmI	CCANNNNNNNNNTGG	1 cut(s)	436
XmnI	GAANNNNTTC	1 cut(s)	264
XspI	CTAG	2 cut(s)	108, 732

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.