FvH4_7g32180
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0017314
Physical Location & Seq
Forward (+)
23163037 .. 23163508
472 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32180.t1

Sequence Viewer

Length: 384 bp
ATGTCCATTGAGAAGAGCTTTCTTCAGTCAAATGATAGAAACATTGTTGTGAAGCTCTGCACCAAGGGTTGTGACATTGTTACTGTTACAGCCAAGACAGATGCCAATGGCATTCTCAGTTTGTCTGCTCAAGGCAATGATGACCAGGATTTGTCGATCACTGGTGATACTGGAAGATTAAATGCTATGGAAATTACTGAAATGTTGGAAGAAGCTGCAAAACACAGGTCAGAAGATGAGGAAATATGGAAGAAAGCTAAGAGCATATATCTGAGCAGGAATGTAATTCAGTTGGAGGTGGGTCCTGAGACTTTTGAGGAGGTTCCTGAGACTTTGGAGGAAGTTACTGCAAATTTGGATGAGGATACTGTTAAACTCACCTAA

Protein Analysis

128

Amino Acids

14.08

Weight (kDa)

4.38

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 21 - 86 6.1e-06 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 352
AcuI CTGAAG 1 cut(s) 8
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 4 cut(s) 18, 55, 215, 257
AluI AGCT 4 cut(s) 18, 55, 215, 257
Alw26I GTCTC 2 cut(s) 302, 323
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 176, 370
AvaII GGWCC 1 cut(s) 302
BbvI GCAGC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 146
BciVI GTATCC 1 cut(s) 358
BcoDI GTCTC 2 cut(s) 302, 323
BfuI GTATCC 1 cut(s) 358
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 303, 324
BmrFI CCNGG 1 cut(s) 146
BmsI GCATC 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 63
Bse1I ACTGG 2 cut(s) 166, 175
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 63
BseGI GGATG 1 cut(s) 364
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 4 cut(s) 130, 263, 297, 318
BseNI ACTGG 2 cut(s) 166, 175
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 1 cut(s) 202
BsgI GTGCAG 1 cut(s) 43
BsmAI GTCTC 2 cut(s) 302, 323
BsmI GAATGC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 156
BspCNI CTCAG 4 cut(s) 129, 264, 298, 319
BspLI GGNNCC 2 cut(s) 303, 324
BspQI GCTCTTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 142
BsrI ACTGG 2 cut(s) 166, 175
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 85, 370
Bst6I CTCTTC 1 cut(s) 8
BstDEI CTNAG 5 cut(s) 116, 258, 272, 306, 327
BstF5I GGATG 1 cut(s) 364
BstKTI GATC 1 cut(s) 159
BstMAI GTCTC 2 cut(s) 302, 323
BstMBI GATC 1 cut(s) 156
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 202
BsuI GTATCC 1 cut(s) 358
BtsCI GGATG 1 cut(s) 364
BtsIMutI CAGTG 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 302
CviJI RGCY 5 cut(s) 18, 55, 92, 215, 257
CviKI_1 RGCY 5 cut(s) 18, 55, 92, 215, 257
DdeI CTNAG 5 cut(s) 116, 258, 272, 306, 327
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 8
EcoO109I RGGNCCY 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 63
FaiI YATR 4 cut(s) 188, 247, 266, 268
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 1 cut(s) 371
Fsp4HI GCNGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 216
HphI GGTGA 2 cut(s) 176, 370
Hpy188I TCNGA 2 cut(s) 232, 273
Hpy188III TCNNGA 2 cut(s) 305, 326
HpyCH4III ACNGT 2 cut(s) 85, 370
HpyCH4V TGCA 3 cut(s) 60, 218, 350
HpyF3I CTNAG 5 cut(s) 116, 258, 272, 306, 327
Kzo9I GATC 1 cut(s) 156
LguI GCTCTTC 1 cut(s) 8
LpnPI CCDG 8 cut(s) 131, 147, 156, 158, 211, 262, 318, 339
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 1 cut(s) 91
MaeIII GTNAC 4 cut(s) 71, 79, 85, 343
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MboII GAAGA 6 cut(s) 14, 25, 186, 221, 245, 262
MluCI AATT 3 cut(s) 192, 285, 352
MmeI TCCRAC 2 cut(s) 186, 273
MnlI CCTC 6 cut(s) 232, 289, 310, 313, 331, 355
MseI TTAA 2 cut(s) 179, 372
MslI CAYNNNNRTG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 111
MvaI CCWGG 1 cut(s) 146
NdeII GATC 1 cut(s) 156
NlaIV GGNNCC 2 cut(s) 303, 324
NmuCI GTSAC 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 8
PctI GAATGC 1 cut(s) 111
PkrI GCNGC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 2 cut(s) 303, 324
PspPI GGNCC 1 cut(s) 302
PspPPI RGGWCCY 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 2 cut(s) 179, 372
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 146
SetI ASST 8 cut(s) 20, 57, 217, 230, 259, 300, 324, 383
SfaNI GCATC 1 cut(s) 91
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 47
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 3 cut(s) 192, 285, 352
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 2 cut(s) 85, 370
TaqI TCGA 1 cut(s) 155
TasI AATT 3 cut(s) 192, 285, 352
Tru1I TTAA 2 cut(s) 179, 372
Tru9I TTAA 2 cut(s) 179, 372
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 71
TspRI CASTG 1 cut(s) 166
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.