Rorug01G0431200
HSP70 Family

Belongs to the heat shock protein 70 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017314
Physical Location & Seq
Forward (+)
52935007 .. 52937861
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0431200.1

Sequence Viewer

Length: 1329 bp
ATGGCTAACTACAGAAGAGCCAAGCTCGCAATCGACGCCTTTCGCGGCTTCACATCAAGAATCGTAGCTAGATCAGAACCCATCTCCAGAGTCTACTCCACCAAGTCTTCAACTTTGGCCTCAAAACCAGCTAAGTTTTCTGGGTTTTCTCCTCGCTATTCTATTTCACAGAACCCAATTCCGCAAAACCAACTGAGCCCATTTATCGGTTTTGCCAAGAGATATTATCATGTGGATCGGCGGCAGGTGCAGCATTTCCGGCAAAGAGGTCCGCGCCGGTGGGCTGACTGGTTCAAGGACCCGAGGAGGGCGTTTGTTACTATTGTGGTGGGCGGTGGGGTTTTTATCACTGTGTATTTTGGGAACTTGGAGACTGTACCATACACCAAGCGTAGGCATTTTGTAATTCTGTCGACTAATTTGGAGAGGAAGCTGGGAGAGCAGCAGTTCGAGGAGTTGAAAGCCGGGTTTAAGGGGAAGATTTTGCCTCCCATACACCCGGAAAGTGTGAGGGTTAGGCTGATTTCCAAGGATATTATTGATGCGTTGAAGAGAGGGTTGAGGCATGATCAGGGGTGGACTGACATGGGGTATGCATCGGAGGAGGTTGACCCGAAATTCGAAGGCCGGGGGAGTGAGAGTGTGCAGGCATTGATGGAGGGTGGGGAGGGGAAGGTGGAGGAGAATTGGTCACGTGAGGATGAGATTCTTGATGATAAGTGGATTGAGCATAGTAGGAAGAAAGGTAAGGATAGTGGTGTTAAGGATGCCACTTCGCATTTGGTTGATTTGAATTGGGAGGTTTTGGTGGTGGATCAGCCGATTGTCAATGCGTTTTGCTTGCCCGGTGGGAAGATAGTGGTCTTCACAGGGTTGCTTAAGCATTTTAGAAGTGATGCCGAGATAGCTACGGTAATTGGTCATGAGGTTGGGCATGCTGTGGCTCGACACTCTGCAGAGGGCATCACAAAGAACCTGTGGGTTGCAATCCTGCAACTGCTTCTTTATCAGCTTATTTCGCCTGATTTTGTCAACCCATTATCCAATCTTTTCTTAAGGCTTCCTTTTTCCCGAAGGATGGAATTGGAAGCGGATCACATTGGGCTGCTGTTGATTGCTTCTGCTGGATATGATCCAAGAGTGGCTCCAACTGTGTACGAGAAGTTGGGGAAACTCTCTGGTGGAGATTCTGCTTTGAGAGATTATCTTACTACTCATCCATCCGGGAAAAAGAGAGCTGAAATGCTGGCTCGAGCTAACATCATGGAAGAGGCACTAACTATATATCGCGACGTACGAGCAGGACGTGGGGTTGAAGGGTTTCTTTAG

Protein Analysis

442

Amino Acids

49.93

Weight (kDa)

9.58

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M48 PF01435 263 - 417 1.7e-30 Peptidase family M48
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 235
AccI GTMKAC 2 cut(s) 93, 413
AccII CGCG 3 cut(s) 45, 274, 1290
AciI CCGC 6 cut(s) 45, 182, 241, 272, 333, 1091
AclWI GGATC 4 cut(s) 243, 822, 1101, 1127
AcsI RAATTY 1 cut(s) 617
AcvI CACGTG 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 378, 1157, 1296
AfiI CCNNNNNNNGG 4 cut(s) 206, 307, 393, 1078
AflII CTTAAG 2 cut(s) 878, 1054
AgsI TTSAA 6 cut(s) 111, 295, 460, 550, 793, 1316
AjiI CACGTC 1 cut(s) 1307
AluBI AGCT 8 cut(s) 25, 68, 131, 433, 908, 1012, 1238, 1256
AluI AGCT 8 cut(s) 25, 68, 131, 433, 908, 1012, 1238, 1256
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 4 cut(s) 243, 822, 1101, 1127
Ama87I CYCGRG 2 cut(s) 301, 1251
AoxI GGCC 2 cut(s) 117, 625
ApeKI GCWGC 3 cut(s) 250, 442, 1105
ApoI RAATTY 1 cut(s) 617
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
Asp700I GAANNNNTTC 1 cut(s) 1320
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 2 cut(s) 269, 298
AsuC2I CCSGG 5 cut(s) 466, 500, 629, 846, 1225
AsuII TTCGAA 1 cut(s) 621
AvaI CYCGRG 2 cut(s) 301, 1251
AvaII GGWCC 2 cut(s) 269, 298
BanII GRGCYC 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 695
BbsI GAAGAC 2 cut(s) 99, 856
BbvI GCAGC 3 cut(s) 262, 454, 1092
BccI CCATC 4 cut(s) 89, 649, 1072, 1228
BclI TGATCA 1 cut(s) 568
BcnI CCSGG 5 cut(s) 466, 500, 629, 846, 1225
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 2 cut(s) 10, 954
BfrI CTTAAG 2 cut(s) 878, 1054
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 5 cut(s) 46, 242, 251, 443, 1106
BlsI GCNGC 5 cut(s) 47, 243, 252, 444, 1107
Bme1390I CCNGG 5 cut(s) 466, 500, 629, 846, 1225
Bme18I GGWCC 2 cut(s) 269, 298
BmeT110I CYCGRG 2 cut(s) 301, 1251
BmgBI CACGTC 1 cut(s) 1307
BmgT120I GGNCC 2 cut(s) 269, 298
BmiI GGNNCC 2 cut(s) 300, 1146
BmrFI CCNGG 5 cut(s) 466, 500, 629, 846, 1225
BmsI GCATC 5 cut(s) 532, 605, 757, 886, 972
BpiI GAAGAC 2 cut(s) 99, 856
BplI GAGNNNNNCTC 2 cut(s) 9, 41
BpmI CTGGAG 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 621
BpuMI CCSGG 5 cut(s) 466, 500, 629, 846, 1225
BsaAI YACGTR 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 302, 528, 628
BsaXI ACNNNNNCTCC 4 cut(s) 625, 655, 674, 704
Bsc4I CCNNNNNNNGG 4 cut(s) 206, 307, 393, 1078
Bse118I RCCGGY 1 cut(s) 276
Bse1I ACTGG 1 cut(s) 293
BseDI CCNNGG 3 cut(s) 302, 528, 628
BseGI GGATG 5 cut(s) 706, 772, 1083, 1216, 1220
BseLI CCNNNNNNNGG 4 cut(s) 206, 307, 393, 1078
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 5 cut(s) 141, 319, 467, 617, 695
BseXI GCAGC 3 cut(s) 262, 454, 1092
BseYI CCCAGC 1 cut(s) 433
BsgI GTGCAG 2 cut(s) 269, 665
Bsh1236I CGCG 3 cut(s) 45, 274, 1290
BshFI GGCC 2 cut(s) 119, 627
BsiHKCI CYCGRG 2 cut(s) 301, 1251
BsiSI CCGG 7 cut(s) 259, 277, 465, 500, 628, 846, 1224
BsiWI CGTACG 1 cut(s) 1294
BslI CCNNNNNNNGG 4 cut(s) 206, 307, 393, 1078
BsmAI GTCTC 1 cut(s) 365
BsnI GGCC 2 cut(s) 119, 627
BsoBI CYCGRG 2 cut(s) 301, 1251
Bsp119I TTCGAA 1 cut(s) 621
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
Bsp68I TCGCGA 1 cut(s) 1290
BspACI CCGC 6 cut(s) 45, 182, 241, 272, 333, 1091
BspANI GGCC 2 cut(s) 119, 627
BspCNI CTCAG 1 cut(s) 186
BspFNI CGCG 3 cut(s) 45, 274, 1290
BspHI TCATGA 1 cut(s) 922
BspLI GGNNCC 2 cut(s) 300, 1146
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 4 cut(s) 243, 822, 1101, 1127
BspQI GCTCTTC 1 cut(s) 10
BspT104I TTCGAA 1 cut(s) 621
BspTI CTTAAG 2 cut(s) 878, 1054
BsrFI RCCGGY 1 cut(s) 276
BsrI ACTGG 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 276
BssECI CCNNGG 3 cut(s) 302, 528, 628
BssMI GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 528
Bst4CI ACNGT 4 cut(s) 352, 376, 913, 1153
Bst6I CTCTTC 3 cut(s) 10, 545, 1263
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 2 cut(s) 878, 1054
BstBAI YACGTR 1 cut(s) 695
BstBI TTCGAA 1 cut(s) 621
BstC8I GCNNGC 5 cut(s) 27, 648, 842, 936, 1248
BstDEI CTNAG 2 cut(s) 132, 194
BstF5I GGATG 5 cut(s) 706, 772, 1083, 1216, 1220
BstFNI CGCG 3 cut(s) 45, 274, 1290
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 6 cut(s) 74, 238, 571, 817, 1096, 1135
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
BstMWI GCNNNNNNNGC 8 cut(s) 26, 35, 247, 250, 259, 439, 905, 1018
BstNSI RCATGY 1 cut(s) 938
BstSCI CCNGG 5 cut(s) 464, 498, 627, 844, 1223
BstSFI CTRYAG 2 cut(s) 10, 954
BstUI CGCG 3 cut(s) 45, 274, 1290
BstV1I GCAGC 3 cut(s) 262, 454, 1092
BstV2I GAAGAC 2 cut(s) 99, 856
BsuRI GGCC 2 cut(s) 119, 627
BtrI CACGTC 1 cut(s) 1307
BtsCI GGATG 5 cut(s) 706, 772, 1083, 1216, 1220
BtsIMutI CAGTG 1 cut(s) 348
BtuMI TCGCGA 1 cut(s) 1290
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 5 cut(s) 27, 648, 842, 936, 1248
CciI TCATGA 1 cut(s) 922
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 2 cut(s) 269, 298
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 377, 1156, 1295
CviAII CATG 6 cut(s) 230, 566, 586, 923, 935, 1264
CviQI GTAC 3 cut(s) 377, 1156, 1295
DdeI CTNAG 2 cut(s) 132, 194
DpnI GATC 6 cut(s) 73, 237, 570, 816, 1095, 1134
DpnII GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
Eam1104I CTCTTC 3 cut(s) 10, 545, 1263
EarI CTCTTC 3 cut(s) 10, 545, 1263
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 200
Eco47I GGWCC 2 cut(s) 269, 298
Eco72I CACGTG 1 cut(s) 695
Eco88I CYCGRG 2 cut(s) 301, 1251
EcoO109I RGGNCCY 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 528
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 6 cut(s) 233, 569, 589, 926, 938, 1267
FalI AAGNNNNNCTT 3 cut(s) 1048, 1080, 1308
FatI CATG 6 cut(s) 229, 565, 585, 922, 934, 1263
FbaI TGATCA 1 cut(s) 568
FblI GTMKAC 2 cut(s) 93, 413
Fnu4HI GCNGC 5 cut(s) 46, 242, 251, 443, 1106
FokI GGATG 5 cut(s) 713, 779, 1090, 1203, 1207
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 5 cut(s) 46, 242, 251, 443, 1106
FspBI CTAG 1 cut(s) 69
GlaI GCGC 1 cut(s) 275
GluI GCNGC 5 cut(s) 46, 242, 251, 443, 1106
GsaI CCCAGC 1 cut(s) 437
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 2 cut(s) 119, 627
HapII CCGG 7 cut(s) 259, 277, 465, 500, 628, 846, 1224
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 6 cut(s) 233, 569, 589, 926, 938, 1267
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 3 cut(s) 414, 610, 1033
HindII GTYRAC 3 cut(s) 414, 610, 1033
HinfI GANTC 4 cut(s) 60, 90, 706, 1187
HpaII CCGG 7 cut(s) 259, 277, 465, 500, 628, 846, 1224
Hpy166II GTNNAC 6 cut(s) 94, 414, 579, 610, 1033, 1156
Hpy188I TCNGA 2 cut(s) 76, 601
Hpy188III TCNNGA 6 cut(s) 57, 87, 710, 923, 1071, 1289
Hpy8I GTNNAC 6 cut(s) 94, 414, 579, 610, 1033, 1156
Hpy99I CGWCG 2 cut(s) 38, 1295
HpyAV CCTTC 4 cut(s) 617, 667, 1068, 1310
HpyCH4III ACNGT 4 cut(s) 352, 376, 913, 1153
HpyCH4IV ACGT 3 cut(s) 694, 1293, 1306
HpyCH4V TGCA 6 cut(s) 250, 596, 646, 956, 986, 994
HpyF10VI GCNNNNNNNGC 8 cut(s) 26, 35, 247, 250, 259, 439, 905, 1018
HpyF3I CTNAG 2 cut(s) 132, 194
HpySE526I ACGT 3 cut(s) 694, 1293, 1306
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 6 cut(s) 233, 569, 589, 926, 938, 1267
HspAI GCGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 568
Kzo9I GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
LguI GCTCTTC 1 cut(s) 10
LmnI GCTCC 1 cut(s) 1150
Lsp1109I GCAGC 3 cut(s) 262, 454, 1092
LweI GCATC 5 cut(s) 532, 605, 757, 886, 972
MaeI CTAG 1 cut(s) 69
MaeII ACGT 3 cut(s) 694, 1293, 1306
MaeIII GTNAC 2 cut(s) 316, 690
MalI GATC 6 cut(s) 73, 237, 570, 816, 1095, 1134
MboI GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
MboII GAAGA 8 cut(s) 27, 99, 490, 562, 751, 856, 865, 1280
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 8 cut(s) 177, 405, 418, 617, 685, 793, 915, 1082
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 1172
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 1320
MseI TTAA 4 cut(s) 471, 762, 879, 1055
MspCI CTTAAG 2 cut(s) 878, 1054
MspI CCGG 7 cut(s) 259, 277, 465, 500, 628, 846, 1224
MspR9I CCNGG 5 cut(s) 466, 500, 629, 846, 1225
MvnI CGCG 3 cut(s) 45, 274, 1290
MwoI GCNNNNNNNGC 8 cut(s) 26, 35, 247, 250, 259, 439, 905, 1018
NciI CCSGG 5 cut(s) 466, 500, 629, 846, 1225
NdeII GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
NlaIII CATG 6 cut(s) 233, 569, 589, 926, 938, 1267
NlaIV GGNNCC 2 cut(s) 300, 1146
NmeAIII GCCGAG 1 cut(s) 925
NmuCI GTSAC 1 cut(s) 690
NruI TCGCGA 1 cut(s) 1290
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 938
NspV TTCGAA 1 cut(s) 621
PaeI GCATGC 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 1251
PagI TCATGA 1 cut(s) 922
PaqCI CACCTGC 1 cut(s) 235
PciSI GCTCTTC 1 cut(s) 10
PcsI WCGNNNNNNNCGW 2 cut(s) 1294, 1303
PdmI GAANNNNTTC 1 cut(s) 1320
PfeI GAWTC 3 cut(s) 60, 706, 1187
Pfl23II CGTACG 1 cut(s) 1294
PfoI TCCNGGA 1 cut(s) 1223
PkrI GCNGC 5 cut(s) 47, 243, 252, 444, 1107
PleI GAGTC 1 cut(s) 98
PmaCI CACGTG 1 cut(s) 695
PmlI CACGTG 1 cut(s) 695
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 298
PspCI CACGTG 1 cut(s) 695
PspFI CCCAGC 1 cut(s) 433
PspLI CGTACG 1 cut(s) 1294
PspN4I GGNNCC 2 cut(s) 300, 1146
PspPI GGNCC 2 cut(s) 269, 298
PspPPI RGGWCCY 1 cut(s) 298
PspXI VCTCGAGB 1 cut(s) 1251
PstI CTGCAG 1 cut(s) 958
RruI TCGCGA 1 cut(s) 1290
RsaI GTAC 3 cut(s) 378, 1157, 1296
RsaNI GTAC 3 cut(s) 377, 1156, 1295
SalI GTCGAC 1 cut(s) 412
SapI GCTCTTC 1 cut(s) 10
SaqAI TTAA 4 cut(s) 471, 762, 879, 1055
SatI GCNGC 5 cut(s) 46, 242, 251, 443, 1106
Sau3AI GATC 6 cut(s) 71, 235, 568, 814, 1093, 1132
Sau96I GGNCC 2 cut(s) 269, 298
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 5 cut(s) 466, 500, 629, 846, 1225
SduI GDGCHC 1 cut(s) 200
SfaNI GCATC 5 cut(s) 532, 605, 757, 886, 972
SfcI CTRYAG 2 cut(s) 10, 954
Sfr274I CTCGAG 1 cut(s) 1251
SfuI TTCGAA 1 cut(s) 621
SgrAI CRCCGGYG 1 cut(s) 276
SinI GGWCC 2 cut(s) 269, 298
SlaI CTCGAG 1 cut(s) 1251
SmlI CTYRAG 3 cut(s) 878, 1054, 1251
SmoI CTYRAG 3 cut(s) 878, 1054, 1251
SphI GCATGC 1 cut(s) 938
Sse9I AATT 8 cut(s) 177, 405, 418, 617, 685, 793, 915, 1082
SsiI CCGC 6 cut(s) 45, 182, 241, 272, 333, 1091
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 5 cut(s) 464, 498, 627, 844, 1223
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 4 cut(s) 352, 376, 913, 1153
TaiI ACGT 3 cut(s) 697, 1296, 1309
TaqI TCGA 6 cut(s) 33, 413, 450, 621, 946, 1252
TasI AATT 8 cut(s) 177, 405, 418, 617, 685, 793, 915, 1082
TauI GCSGC 2 cut(s) 48, 244
TfiI GAWTC 3 cut(s) 60, 706, 1187
Tru1I TTAA 4 cut(s) 471, 762, 879, 1055
Tru9I TTAA 4 cut(s) 471, 762, 879, 1055
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 1 cut(s) 690
TseI GCWGC 3 cut(s) 250, 442, 1105
Tsp45I GTSAC 1 cut(s) 690
TspRI CASTG 1 cut(s) 355
Vha464I CTTAAG 2 cut(s) 878, 1054
VpaK11BI GGWCC 2 cut(s) 269, 298
XapI RAATTY 1 cut(s) 617
XceI RCATGY 1 cut(s) 938
XcmI CCANNNNNNNNNTGG 1 cut(s) 778
XhoI CTCGAG 1 cut(s) 1251
XmiI GTMKAC 2 cut(s) 93, 413
XmnI GAANNNNTTC 1 cut(s) 1320
XspI CTAG 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.