FvH4_7g32930

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015397
Physical Location & Seq
Forward (+)
23575278 .. 23576894
1617 bp
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UTR
Exon/CDS
Intron
FvH4_7g32930.t1

Sequence Viewer

Length: 639 bp
ATGTCTCAAGCCCTCTCGAAATCTCCTAAACACTGTGGCGATAAGCAAGGACTGAAAATTAACAAGCACTGCAAGAAGCTATTTGCAGCATTCTCTACACTATTCACTACAATCCTCTGTTTAATACTACTTGTCTGGCTTATTCTACACCCCACCAAGCCTCAGTTCTCTCTCAAAGAAGCTGGTATCTACCTCCTCAACCTCTCCGGAAGCAATAACCTCCTCAACTCCTCCGTCCAACTCACCCTGCTTTCCAAGAACCCCAATCAAAAAGTTGGCATTTACTACGACGAGCTCAAAGTATATGCTCAATACAAAGGCCAACAGATCACAGTTTACACATCTCTGCCTCCTTTCTACCAAGGACATGAAGACAGCAATCTCCTCACAGTTTCTTTGATTGGAAATGGTCTGCCTGTGGCTCCCTCTTTCGGTTATGAAGTGGGACGTGATCAGACGGCTGGGAGACTTGTATTGAATCTCAAAGTGCTCGGACGACTAAGGTGGAAGGTAGGGACTTGGGTGTCCGGGAGATACAGAGTGAACGTGGATTGCGTTGCTGTTATGGCTTTCGGTCCTTCTCTCCCATCAGGGCCACTCACTACTAGGCAGGGGACTCAGTGTTCTACCACTGTCTGA

Protein Analysis

213

Amino Acids

23.33

Weight (kDa)

9.68

Isoelectric Point (pI)

28.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 82 - 185 1.6e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53730
fragaria_vesca FvH4_7g32930
malus_domestica MD01G1228300.v1.1 MD07G1299200.v1.1
prunus_persica Prupe.2G319000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0382891
rosa_laevigata RLG00000026151
rosa_multiflora Rmu_co8420301.1_g000001 Rmu_sc0010366.1_g000005
rosa_roxburghii Rroxscaffold_4G00277800
rosa_rugosa Rorug01G0437100
rosa_samantha Rh1AG462100 Rh1BG419200 Rh1CG432800 Rh1DG451200
rosa_wichuraiana Rw1G040970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 523
AccIII TCCGGA 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 431
AgsI TTSAA 1 cut(s) 478
AjiI CACGTC 1 cut(s) 449
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AloI GAACNNNNNNTCC 2 cut(s) 607, 639
AluBI AGCT 3 cut(s) 79, 182, 295
AluI AGCT 3 cut(s) 79, 182, 295
Alw21I GWGCWC 2 cut(s) 297, 492
Alw26I GTCTC 2 cut(s) 9, 460
Aor13HI TCCGGA 1 cut(s) 206
AoxI GGCC 2 cut(s) 319, 593
ApeKI GCWGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 575, 593
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 575
BanII GRGCYC 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 2 cut(s) 297, 492
BbvI GCAGC 1 cut(s) 98
BccI CCATC 1 cut(s) 595
BceAI ACGGC 1 cut(s) 474
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 2 cut(s) 9, 460
BfaI CTAG 1 cut(s) 606
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 1 cut(s) 529
Bme18I GGWCC 1 cut(s) 575
BmgBI CACGTC 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 575, 593
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 529
BsaJI CCNNGG 1 cut(s) 361
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 431
BseAI TCCGGA 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMII CTCAG 2 cut(s) 176, 632
BseRI GAGGAG 4 cut(s) 185, 212, 220, 374
BseXI GCAGC 1 cut(s) 98
BseYI CCCAGC 1 cut(s) 461
BshFI GGCC 2 cut(s) 321, 595
BsiHKAI GWGCWC 2 cut(s) 297, 492
BsiSI CCGG 2 cut(s) 207, 528
BslFI GGGAC 3 cut(s) 459, 529, 628
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 2 cut(s) 9, 460
BsmFI GGGAC 3 cut(s) 459, 529, 628
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 2 cut(s) 321, 595
Bsp1286I GDGCHC 2 cut(s) 297, 492
Bsp13I TCCGGA 1 cut(s) 206
Bsp143I GATC 2 cut(s) 327, 451
BspANI GGCC 2 cut(s) 321, 595
BspCNI CTCAG 2 cut(s) 175, 631
BspEI TCCGGA 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 423
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 2 cut(s) 327, 451
BssT1I CCWWGG 1 cut(s) 361
Bst4CI ACNGT 4 cut(s) 35, 334, 391, 634
BstDEI CTNAG 3 cut(s) 162, 500, 618
BstKTI GATC 2 cut(s) 330, 454
BstMAI GTCTC 2 cut(s) 9, 460
BstMBI GATC 2 cut(s) 327, 451
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 527
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 378
BsuRI GGCC 2 cut(s) 321, 595
BtrI CACGTC 1 cut(s) 449
BtsI GCAGTG 1 cut(s) 67
BtsIMutI CAGTG 4 cut(s) 31, 67, 626, 630
Cfr13I GGNCC 2 cut(s) 575, 593
CviAII CATG 1 cut(s) 368
DdeI CTNAG 3 cut(s) 162, 500, 618
DpnI GATC 2 cut(s) 329, 453
DpnII GATC 2 cut(s) 327, 451
DrdI GACNNNNNNGTC 1 cut(s) 523
DseDI GACNNNNNNGTC 1 cut(s) 523
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 575
Eco53kI GAGCTC 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 361
FaeI CATG 1 cut(s) 371
FaiI YATR 5 cut(s) 304, 306, 369, 438, 566
FaqI GGGAC 3 cut(s) 459, 529, 628
FatI CATG 1 cut(s) 367
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 1 cut(s) 87
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 1 cut(s) 606
GluI GCNGC 1 cut(s) 87
GsaI CCCAGC 1 cut(s) 465
HaeIII GGCC 2 cut(s) 321, 595
HapII CCGG 2 cut(s) 207, 528
Hin1II CATG 1 cut(s) 371
HinfI GANTC 2 cut(s) 478, 616
HpaII CCGG 2 cut(s) 207, 528
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 2 cut(s) 337, 544
Hpy188I TCNGA 3 cut(s) 456, 494, 638
Hpy188III TCNNGA 2 cut(s) 16, 207
Hpy8I GTNNAC 2 cut(s) 337, 544
Hpy99I CGWCG 1 cut(s) 293
HpyAV CCTTC 2 cut(s) 502, 588
HpyCH4III ACNGT 4 cut(s) 35, 334, 391, 634
HpyCH4IV ACGT 2 cut(s) 448, 546
HpyCH4V TGCA 2 cut(s) 72, 86
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpyF3I CTNAG 3 cut(s) 162, 500, 618
HpySE526I ACGT 2 cut(s) 448, 546
Hsp92II CATG 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 2 cut(s) 327, 451
LmnI GCTCC 1 cut(s) 427
LpnPI CCDG 9 cut(s) 121, 168, 220, 260, 429, 447, 541, 576, 596
Lsp1109I GCAGC 1 cut(s) 98
MaeI CTAG 1 cut(s) 606
MaeII ACGT 2 cut(s) 448, 546
MalI GATC 2 cut(s) 329, 453
MboI GATC 2 cut(s) 327, 451
MboII GAAGA 1 cut(s) 383
MhlI GDGCHC 2 cut(s) 297, 492
MluCI AATT 1 cut(s) 57
MlyI GAGTC 1 cut(s) 610
MmeI TCCRAC 1 cut(s) 262
MroI TCCGGA 1 cut(s) 206
MseI TTAA 2 cut(s) 60, 122
MspI CCGG 2 cut(s) 207, 528
MspR9I CCNGG 1 cut(s) 529
Mva1269I GAATGC 1 cut(s) 89
MwoI GCNNNNNNNGC 1 cut(s) 566
NciI CCSGG 1 cut(s) 529
NdeII GATC 2 cut(s) 327, 451
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 1 cut(s) 423
PcsI WCGNNNNNNNCGW 1 cut(s) 552
PctI GAATGC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 478
PfoI TCCNGGA 1 cut(s) 527
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
Psp124BI GAGCTC 1 cut(s) 297
PspFI CCCAGC 1 cut(s) 461
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 2 cut(s) 575, 593
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 60, 122
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 2 cut(s) 327, 451
Sau96I GGNCC 2 cut(s) 575, 593
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 1 cut(s) 529
SduI GDGCHC 2 cut(s) 297, 492
SinI GGWCC 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 1 cut(s) 57
SspMI CTAG 1 cut(s) 606
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 527
StyI CCWWGG 1 cut(s) 361
TaaI ACNGT 4 cut(s) 35, 334, 391, 634
TaiI ACGT 2 cut(s) 451, 549
TaqI TCGA 1 cut(s) 17
TaqII GACCGA 1 cut(s) 563
TasI AATT 1 cut(s) 57
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 2 cut(s) 60, 122
Tru9I TTAA 2 cut(s) 60, 122
TscAI CASTG 4 cut(s) 38, 74, 626, 637
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 384, 453
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 4 cut(s) 38, 74, 626, 637
VpaK11BI GGWCC 1 cut(s) 575
XspI CTAG 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.