MD01G1228300.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0015397
Physical Location & Seq
Forward (+)
31816155 .. 31816790
636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1228300.v1.1.491

Sequence Viewer

Length: 636 bp
ATGTCTCAAGCCCTCACAAAATCTCCTAAACACTGTGGCAACAAACACGGACTTAACATTTGCAAGCTCTACAAGAATCTTTTCTTACTATTCTCTACATTTGGCACCACAATTCTCTCCATAATACTAGTCGTTTGGCTTATCCTCCGCCCCACGAAACCTGAGTTCTCCCTCAAGGAAGCCGATATCTACCAGCTCAACCTCTTCAGCAGCTACCTCCTCAACTCCTCCGTCCAACTCACCCTACTTTCAAAAAACCCTAATCAGAAAGTCGGGATTTACTACGACGAGCTTCAAGTCTATGCTGCATACAAGGGCCAGCAAATCACTGTCTACACCTCTCTTCCTCCGTTTTACCAAGGACATGAAGACAGTAATATCTTGACAGCTTCTTTGGTGGGAATCGGTCTGCCAGTGGGTCCCTCTTTCGGTTATGAAATGGGACGTGATCAAACGGCTGGTAGACTGGTTTTGAATCTCAAAGTGATTGGACGACTTCGATGGAAGGTCGGAGTTTGGGTTTCCGGGAAGTATAGGGTCAACGTGGATTGTCTTGCTGTCATGGCTTTTGGTCCTGCTGCTCCAACTGGTCCTCTCACTACAAGGCAAGGGACTCAGTGTTCTACCAACGTCTGA

Protein Analysis

212

Amino Acids

23.34

Weight (kDa)

9.51

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 81 - 184 5.4e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53730
fragaria_vesca FvH4_7g32930
malus_domestica MD01G1228300.v1.1 MD07G1299200.v1.1
prunus_persica Prupe.2G319000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0382891
rosa_laevigata RLG00000026151
rosa_multiflora Rmu_co8420301.1_g000001 Rmu_sc0010366.1_g000005
rosa_roxburghii Rroxscaffold_4G00277800
rosa_rugosa Rorug01G0437100
rosa_samantha Rh1AG462100 Rh1BG419200 Rh1CG432800 Rh1DG451200
rosa_wichuraiana Rw1G040970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 2 cut(s) 333, 463
AciI CCGC 1 cut(s) 148
AcuI CTGAAG 1 cut(s) 190
AfiI CCNNNNNNNGG 1 cut(s) 428
AgsI TTSAA 3 cut(s) 252, 296, 475
AhlI ACTAGT 1 cut(s) 127
AjiI CACGTC 1 cut(s) 446
AloI GAACNNNNNNTCC 2 cut(s) 604, 636
AluBI AGCT 5 cut(s) 67, 196, 213, 292, 389
AluI AGCT 5 cut(s) 67, 196, 213, 292, 389
Alw26I GTCTC 1 cut(s) 9
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 3 cut(s) 210, 305, 578
ArsI GACNNNNNNTTYG 4 cut(s) 42, 74, 376, 408
Asp700I GAANNNNTTC 1 cut(s) 80
AspS9I GGNCC 4 cut(s) 316, 419, 572, 590
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 232
AvaII GGWCC 3 cut(s) 419, 572, 590
BanI GGYRCC 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 375
BbvI GCAGC 3 cut(s) 222, 292, 565
BccI CCATC 1 cut(s) 495
BceAI ACGGC 1 cut(s) 471
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 9
BcuI ACTAGT 1 cut(s) 127
BfaI CTAG 1 cut(s) 128
BisI GCNGC 3 cut(s) 211, 306, 579
BlsI GCNGC 3 cut(s) 212, 307, 580
Bme1390I CCNGG 1 cut(s) 526
Bme18I GGWCC 3 cut(s) 419, 572, 590
BmgBI CACGTC 1 cut(s) 446
BmgT120I GGNCC 4 cut(s) 316, 419, 572, 590
BmiI GGNNCC 3 cut(s) 106, 420, 421
BmrFI CCNGG 1 cut(s) 526
BpiI GAAGAC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 526
BsaJI CCNNGG 1 cut(s) 358
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 504, 534
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 3 cut(s) 413, 471, 592
BseDI CCNNGG 1 cut(s) 358
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 153, 629
BseNI ACTGG 3 cut(s) 413, 471, 592
BseRI GAGGAG 2 cut(s) 209, 217
BseXI GCAGC 3 cut(s) 222, 292, 565
BshFI GGCC 1 cut(s) 318
BshNI GGYRCC 1 cut(s) 104
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 3 cut(s) 405, 456, 625
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 3 cut(s) 405, 456, 625
BsnI GGCC 1 cut(s) 318
Bsp143I GATC 1 cut(s) 448
BspACI CCGC 1 cut(s) 148
BspANI GGCC 1 cut(s) 318
BspCNI CTCAG 2 cut(s) 154, 628
BspLI GGNNCC 3 cut(s) 106, 420, 421
BspT107I GGYRCC 1 cut(s) 104
BsrI ACTGG 3 cut(s) 413, 471, 592
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 1 cut(s) 448
BssT1I CCWWGG 1 cut(s) 358
Bst4CI ACNGT 3 cut(s) 35, 331, 374
Bst6I CTCTTC 2 cut(s) 209, 348
BstC8I GCNNGC 2 cut(s) 65, 320
BstDEI CTNAG 2 cut(s) 162, 615
BstKTI GATC 1 cut(s) 451
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstSCI CCNGG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 222, 292, 565
BstV2I GAAGAC 1 cut(s) 375
BsuRI GGCC 1 cut(s) 318
BtrI CACGTC 1 cut(s) 446
BtsIMutI CAGTG 4 cut(s) 31, 327, 420, 623
Cac8I GCNNGC 2 cut(s) 65, 320
Cfr13I GGNCC 4 cut(s) 316, 419, 572, 590
CviAII CATG 2 cut(s) 365, 562
DdeI CTNAG 2 cut(s) 162, 615
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
Eam1104I CTCTTC 2 cut(s) 209, 348
EarI CTCTTC 2 cut(s) 209, 348
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 358
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 3 cut(s) 419, 572, 590
Eco57I CTGAAG 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 419
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 358
FaeI CATG 2 cut(s) 368, 565
FaiI YATR 7 cut(s) 122, 303, 310, 366, 435, 534, 563
FaqI GGGAC 3 cut(s) 405, 456, 625
FatI CATG 2 cut(s) 364, 561
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 2 cut(s) 333, 463
Fnu4HI GCNGC 3 cut(s) 211, 306, 579
Fsp4HI GCNGC 3 cut(s) 211, 306, 579
FspBI CTAG 1 cut(s) 128
GluI GCNGC 3 cut(s) 211, 306, 579
HaeIII GGCC 1 cut(s) 318
HapII CCGG 1 cut(s) 525
Hin1II CATG 2 cut(s) 368, 565
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 4 cut(s) 76, 402, 475, 613
HpaII CCGG 1 cut(s) 525
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 3 cut(s) 334, 464, 541
Hpy188I TCNGA 3 cut(s) 267, 512, 635
Hpy188III TCNNGA 2 cut(s) 274, 382
Hpy8I GTNNAC 3 cut(s) 334, 464, 541
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 3 cut(s) 35, 331, 374
HpyCH4IV ACGT 3 cut(s) 445, 543, 630
HpyCH4V TGCA 2 cut(s) 63, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 2 cut(s) 162, 615
HpySE526I ACGT 3 cut(s) 445, 543, 630
Hsp92II CATG 2 cut(s) 368, 565
KflI GGGWCCC 1 cut(s) 419
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 1 cut(s) 586
LpnPI CCDG 9 cut(s) 174, 206, 332, 426, 444, 452, 538, 573, 588
Lsp1109I GCAGC 3 cut(s) 222, 292, 565
MaeI CTAG 1 cut(s) 128
MaeII ACGT 3 cut(s) 445, 543, 630
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 3 cut(s) 196, 335, 380
MluCI AATT 1 cut(s) 111
MlyI GAGTC 1 cut(s) 607
MmeI TCCRAC 3 cut(s) 259, 490, 608
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 1 cut(s) 54
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 526
MwoI GCNNNNNNNGC 1 cut(s) 563
NciI CCSGG 1 cut(s) 526
NdeII GATC 1 cut(s) 448
NlaIII CATG 2 cut(s) 368, 565
NlaIV GGNNCC 3 cut(s) 106, 420, 421
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 3 cut(s) 76, 402, 475
PfoI TCCNGGA 1 cut(s) 524
PkrI GCNGC 3 cut(s) 212, 307, 580
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
PpuMI RGGWCCY 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 419
PspN4I GGNNCC 3 cut(s) 106, 420, 421
PspPI GGNCC 4 cut(s) 316, 419, 572, 590
PspPPI RGGWCCY 1 cut(s) 419
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 3 cut(s) 211, 306, 579
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 4 cut(s) 316, 419, 572, 590
SchI GAGTC 1 cut(s) 607
ScrFI CCNGG 1 cut(s) 526
SinI GGWCC 3 cut(s) 419, 572, 590
SmlI CTYRAG 2 cut(s) 6, 173
SmoI CTYRAG 2 cut(s) 6, 173
SpeI ACTAGT 1 cut(s) 127
Sse9I AATT 1 cut(s) 111
SsiI CCGC 1 cut(s) 148
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 524
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 3 cut(s) 35, 331, 374
TaiI ACGT 3 cut(s) 448, 546, 633
TaqI TCGA 1 cut(s) 499
TaqII GACCGA 1 cut(s) 395
TasI AATT 1 cut(s) 111
TfiI GAWTC 3 cut(s) 76, 402, 475
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 4 cut(s) 38, 334, 420, 623
TseI GCWGC 3 cut(s) 210, 305, 578
TspDTI ATGAA 2 cut(s) 381, 450
TspGWI ACGGA 3 cut(s) 63, 220, 339
TspRI CASTG 4 cut(s) 38, 334, 420, 623
VpaK11BI GGWCC 3 cut(s) 419, 572, 590
XmiI GTMKAC 2 cut(s) 333, 463
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.