MD07G1299200.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015397
Physical Location & Seq
Forward (+)
35844167 .. 35844802
636 bp
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UTR
Exon/CDS
Intron
MD07G1299200.v1.1.491

Sequence Viewer

Length: 636 bp
ATGTCTGAAGCCCTCACAAAATCTCCTAAACACTGTGGCAACAAACAAGGACTTAACATTAGCAAGCTCTACAGGAAGCTTTTCTTACTATTCTCTACATTTGCCACCACAATTCTCTCCATAATACTACTCGTCTGGCTCATCCTTCGCCCCACGAAACCCGAGTTCTCCCTCAAAGAAGCCGATATCTACCAGCTGAACCTCTCCGGCGGTCACCTCCTCAACTCGTCCGTCCAACTCACCCTCCTTTCGAAAAACCCTAATCAGAAAGTCGGGATTTACTATGACGAGCTTAAAGTCTACGCTGTATACAAGGGCCAGCAAATCACTCTTTACACTTCTCTTCCTCCGTTTTACCAAGGACATGAAGACACCAATGTCTTGACAGCTTCTCTGGTGGGAATAGGTCTGCCGGTGGATCCCTCGTTTGGCTATGAAGTGGGACGTGATCAAACGGCTGGTAGACTGGTTTTGAATCTCAAAGTGATAGGACGAATTCGATGGAAGGTTGGAACTTGGGTTTCCGGGAAATATCGGGTTACCGTGGATTGTCTTGCTGTTATGGCTTTTGGTCCTTATGTTCCAACTGGTCCTCTCACTGCAAGGCAAGGGACTCAATGCTCTACCAACGTCTGA

Protein Analysis

212

Amino Acids

23.39

Weight (kDa)

9.56

Isoelectric Point (pI)

26.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 81 - 184 9.9e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53730
fragaria_vesca FvH4_7g32930
malus_domestica MD01G1228300.v1.1 MD07G1299200.v1.1
prunus_persica Prupe.2G319000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0382891
rosa_laevigata RLG00000026151
rosa_multiflora Rmu_co8420301.1_g000001 Rmu_sc0010366.1_g000005
rosa_roxburghii Rroxscaffold_4G00277800
rosa_rugosa Rorug01G0437100
rosa_samantha Rh1AG462100 Rh1BG419200 Rh1CG432800 Rh1DG451200
rosa_wichuraiana Rw1G040970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
AccI GTMKAC 3 cut(s) 300, 309, 463
AciI CCGC 1 cut(s) 210
AclWI GGATC 2 cut(s) 413, 426
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 428
AgsI TTSAA 1 cut(s) 475
AjiI CACGTC 1 cut(s) 446
AluBI AGCT 5 cut(s) 67, 79, 196, 292, 389
AluI AGCT 5 cut(s) 67, 79, 196, 292, 389
AlwI GGATC 2 cut(s) 413, 426
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 1 cut(s) 316
ApoI RAATTY 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 80
AspS9I GGNCC 3 cut(s) 316, 572, 590
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 2 cut(s) 206, 232
AsuII TTCGAA 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 2 cut(s) 572, 590
BamHI GGATCC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 375
BccI CCATC 1 cut(s) 495
BceAI ACGGC 1 cut(s) 471
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 1 cut(s) 526
BfmI CTRYAG 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 526
Bme18I GGWCC 2 cut(s) 572, 590
BmeT110I CYCGRG 1 cut(s) 161
BmgBI CACGTC 1 cut(s) 446
BmgT120I GGNCC 3 cut(s) 316, 572, 590
BmiI GGNNCC 1 cut(s) 420
BmrFI CCNGG 1 cut(s) 526
BpiI GAAGAC 1 cut(s) 375
Bpu14I TTCGAA 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 526
BsaJI CCNNGG 2 cut(s) 358, 543
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 228, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse118I RCCGGY 1 cut(s) 412
Bse1I ACTGG 2 cut(s) 471, 592
BseDI CCNNGG 2 cut(s) 358, 543
BseGI GGATG 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 428
BseNI ACTGG 2 cut(s) 471, 592
BseRI GAGGAG 1 cut(s) 209
BshFI GGCC 1 cut(s) 318
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 207, 413, 525
BslFI GGGAC 2 cut(s) 456, 625
BslI CCNNNNNNNGG 1 cut(s) 428
BsmFI GGGAC 2 cut(s) 456, 625
BsnI GGCC 1 cut(s) 318
BsoBI CYCGRG 1 cut(s) 161
Bsp119I TTCGAA 1 cut(s) 251
Bsp143I GATC 2 cut(s) 418, 448
BspACI CCGC 1 cut(s) 210
BspANI GGCC 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 420
BspPI GGATC 2 cut(s) 413, 426
BspT104I TTCGAA 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 412
BsrI ACTGG 2 cut(s) 471, 592
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 2 cut(s) 358, 543
BssMI GATC 2 cut(s) 418, 448
BssNAI GTATAC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 358
Bst1107I GTATAC 1 cut(s) 310
Bst4CI ACNGT 2 cut(s) 35, 544
Bst6I CTCTTC 1 cut(s) 348
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 2 cut(s) 65, 320
BstDSI CCRYGG 1 cut(s) 543
BstEII GGTNACC 2 cut(s) 212, 538
BstF5I GGATG 1 cut(s) 141
BstKTI GATC 2 cut(s) 421, 451
BstMBI GATC 2 cut(s) 418, 448
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstPI GGTNACC 2 cut(s) 212, 538
BstSCI CCNGG 1 cut(s) 524
BstSFI CTRYAG 1 cut(s) 70
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BstZ17I GTATAC 1 cut(s) 310
BsuRI GGCC 1 cut(s) 318
BtgI CCRYGG 1 cut(s) 543
BtrI CACGTC 1 cut(s) 446
BtsCI GGATG 1 cut(s) 141
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 31, 597
Cac8I GCNNGC 2 cut(s) 65, 320
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 3 cut(s) 316, 572, 590
CviAII CATG 1 cut(s) 365
DpnI GATC 2 cut(s) 420, 450
DpnII GATC 2 cut(s) 418, 448
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
Eco130I CCWWGG 1 cut(s) 358
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 2 cut(s) 572, 590
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 161
Eco91I GGTNACC 2 cut(s) 212, 538
EcoO65I GGTNACC 2 cut(s) 212, 538
EcoRI GAATTC 1 cut(s) 495
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 358
FaeI CATG 1 cut(s) 368
FaiI YATR 7 cut(s) 122, 285, 310, 366, 435, 563, 579
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 2 cut(s) 456, 625
FatI CATG 1 cut(s) 364
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 3 cut(s) 300, 309, 463
FokI GGATG 1 cut(s) 128
HaeIII GGCC 1 cut(s) 318
HapII CCGG 3 cut(s) 207, 413, 525
Hin1II CATG 1 cut(s) 368
HindIII AAGCTT 1 cut(s) 77
HinfI GANTC 2 cut(s) 475, 613
HpaII CCGG 3 cut(s) 207, 413, 525
HphI GGTGA 2 cut(s) 206, 232
Hpy166II GTNNAC 3 cut(s) 301, 310, 464
Hpy188I TCNGA 3 cut(s) 7, 267, 635
Hpy188III TCNNGA 2 cut(s) 274, 382
Hpy8I GTNNAC 3 cut(s) 301, 310, 464
HpyAV CCTTC 2 cut(s) 155, 499
HpyCH4III ACNGT 2 cut(s) 35, 544
HpyCH4IV ACGT 2 cut(s) 445, 630
HpyCH4V TGCA 1 cut(s) 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpySE526I ACGT 2 cut(s) 445, 630
Hsp92II CATG 1 cut(s) 368
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 2 cut(s) 418, 448
MaeII ACGT 2 cut(s) 445, 630
MaeIII GTNAC 2 cut(s) 212, 538
MalI GATC 2 cut(s) 420, 450
MboI GATC 2 cut(s) 418, 448
MboII GAAGA 2 cut(s) 335, 380
MflI RGATCY 1 cut(s) 418
MluCI AATT 2 cut(s) 111, 495
MlyI GAGTC 1 cut(s) 607
MmeI TCCRAC 3 cut(s) 259, 490, 608
MnlI CCTC 9 cut(s) 23, 182, 212, 227, 230, 254, 357, 433, 603
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 2 cut(s) 54, 294
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 3 cut(s) 207, 413, 525
MspR9I CCNGG 1 cut(s) 526
MwoI GCNNNNNNNGC 1 cut(s) 563
NciI CCSGG 1 cut(s) 526
NdeII GATC 2 cut(s) 418, 448
NlaIII CATG 1 cut(s) 368
NlaIV GGNNCC 1 cut(s) 420
NmuCI GTSAC 1 cut(s) 212
NspV TTCGAA 1 cut(s) 251
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 475
PfoI TCCNGGA 1 cut(s) 524
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
PspEI GGTNACC 2 cut(s) 212, 538
PspN4I GGNNCC 1 cut(s) 420
PspPI GGNCC 3 cut(s) 316, 572, 590
PsuI RGATCY 1 cut(s) 418
PvuII CAGCTG 1 cut(s) 196
SaqAI TTAA 2 cut(s) 54, 294
Sau3AI GATC 2 cut(s) 418, 448
Sau96I GGNCC 3 cut(s) 316, 572, 590
SchI GAGTC 1 cut(s) 607
ScrFI CCNGG 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 70
SfuI TTCGAA 1 cut(s) 251
SinI GGWCC 2 cut(s) 572, 590
Sse9I AATT 2 cut(s) 111, 495
SsiI CCGC 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 524
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 2 cut(s) 35, 544
TaiI ACGT 2 cut(s) 448, 633
TaqI TCGA 2 cut(s) 251, 499
TasI AATT 2 cut(s) 111, 495
TfiI GAWTC 1 cut(s) 475
Tru1I TTAA 2 cut(s) 54, 294
Tru9I TTAA 2 cut(s) 54, 294
TscAI CASTG 2 cut(s) 38, 604
TseFI GTSAC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 381, 450
TspGWI ACGGA 2 cut(s) 220, 339
TspRI CASTG 2 cut(s) 38, 604
VpaK11BI GGWCC 2 cut(s) 572, 590
XapI RAATTY 1 cut(s) 495
XmiI GTMKAC 3 cut(s) 300, 309, 463
XmnI GAANNNNTTC 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.