MD00G1008800.v1.1

Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0016799
Physical Location & Seq
Forward (+)
1123706 .. 1125282
1577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1008800.v1.1.491

Sequence Viewer

Length: 705 bp
ATGCCGATATTTGGAGCCGTTGCTGCAATTGCAGCGGCTTTTTCGACCGGTGCCAACAATCTTCCGTCTCCGTTTTCGACACCTATTGGGTCGGGGAGTTTGACACTTCTGAAGGCATTTGAAGCAGCCTTTGCCAGTTGCGGTAGTTTTGTGAATGATCTGTTTTCTGATGTACGGATTAAGACACTTCCATTTCAACTCTATTTTACGTCCAAAGAAATTTTTCTCAAGGAAAATCAGCCTAGTGAAGGCTTGTTGATGTGGAGTATGGTAGTTGTTGTAGAAACCGCAGGTATATTCAAAGACTTGCTTATTGCTGATATTTATTTTATACGTTCTTTTTGTCATGCCTATAAATTTTTTACAGCAATTTGGCTTGCTCTGGCTACATATTTGCAGCTGCCAGTCTCACCTCAGCAGTCAATGCAGGGTGCCCTATTGGGAACTATACTTGTTACTGAAGGTTTTAGCTACATACCCTTGCGGAATAAGAATGCAAATCACAATTTCAATCGTGGAGGACTTCTGTGGATATTTCTTGAACGGACTGTTGCTCCATTCATTGCCTTTGCATGTGTACTCTTCCTCTTCAGCGTAATGAAGGCTTCTCTGCTTCACCGTGAAAATGTCGAAAAAAGGTCATACCGTCTACAAGTGCAGTTCACAGTAGGGTTACTGTTGTTGCAGTTGCCCTGGCCGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHO4

Protein Analysis

235

Amino Acids

26.05

Weight (kDa)

9.03

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHO4 PF01384 17 - 217 2.7e-13 Phosphate transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016799)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27970
malus_domestica MD00G1008800.v1.1 MD10G1275200.v1.1
prunus_persica Prupe.4G067200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011761
rosa_laevigata RLG00000031860
rosa_roxburghii Rroxscaffold_1G00064790
rosa_rugosa Rorug04G0458700 Rorug04G0458800
rosa_samantha Rh5AG090500 Rh5BG086000 Rh5CG099800 Rh5DG086200
rosa_wichuraiana Rw5G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 281
AccB1I GGYRCC 2 cut(s) 50, 431
AccI GTMKAC 1 cut(s) 649
AciI CCGC 5 cut(s) 35, 141, 288, 484, 698
AcoI YGGCCR 1 cut(s) 695
AcsI RAATTY 2 cut(s) 219, 356
AcuI CTGAAG 3 cut(s) 131, 480, 574
AfaI GTAC 2 cut(s) 174, 579
AfiI CCNNNNNNNGG 2 cut(s) 11, 248
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 5 cut(s) 122, 197, 301, 511, 542
AjnI CCWGG 1 cut(s) 692
AluBI AGCT 2 cut(s) 400, 471
AluI AGCT 2 cut(s) 400, 471
Alw26I GTCTC 2 cut(s) 72, 412
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 5 cut(s) 23, 32, 125, 397, 400
ApoI RAATTY 2 cut(s) 219, 356
AsiGI ACCGGT 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 402, 608
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 2 cut(s) 50, 431
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 5 cut(s) 10, 44, 137, 387, 409
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 1 cut(s) 694
BcoDI GTCTC 2 cut(s) 72, 412
BfaI CTAG 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 7 cut(s) 24, 33, 36, 126, 398, 401, 698
BlsI GCNGC 7 cut(s) 25, 34, 37, 127, 399, 402, 699
Bme1390I CCNGG 1 cut(s) 694
BmiI GGNNCC 3 cut(s) 16, 52, 433
BmrFI CCNGG 1 cut(s) 694
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 692
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 248
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 2 cut(s) 135, 404
Bse3DI GCAATG 1 cut(s) 561
BseBI CCWGG 1 cut(s) 694
BseDI CCNNGG 1 cut(s) 692
BseLI CCNNNNNNNGG 2 cut(s) 11, 248
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 2 cut(s) 135, 404
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 5 cut(s) 10, 44, 137, 387, 409
BsgI GTGCAG 1 cut(s) 677
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 1 cut(s) 697
BshNI GGYRCC 2 cut(s) 50, 431
BshTI ACCGGT 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 48
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 2 cut(s) 11, 248
BsmAI GTCTC 2 cut(s) 72, 412
BsmBI CGTCTC 1 cut(s) 72
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 1 cut(s) 697
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 1 cut(s) 157
BspACI CCGC 5 cut(s) 35, 141, 288, 484, 698
BspANI GGCC 1 cut(s) 697
BspCNI CTCAG 1 cut(s) 427
BspLI GGNNCC 3 cut(s) 16, 52, 433
BspMI ACCTGC 1 cut(s) 281
BspT107I GGYRCC 2 cut(s) 50, 431
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 2 cut(s) 135, 404
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 692
BssMI GATC 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 694
Bst4CI ACNGT 5 cut(s) 550, 620, 647, 667, 678
Bst6I CTCTTC 2 cut(s) 587, 593
BstAPI GCANNNNNTGC 2 cut(s) 131, 424
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 1 cut(s) 414
BstENI CCTNNNNNAGG 1 cut(s) 246
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 2 cut(s) 72, 412
BstMBI GATC 1 cut(s) 157
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 7 cut(s) 23, 29, 32, 122, 131, 424, 697
BstNI CCWGG 1 cut(s) 694
BstNSI RCATGY 1 cut(s) 576
BstSCI CCNGG 1 cut(s) 692
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 5 cut(s) 10, 44, 137, 387, 409
BsuRI GGCC 1 cut(s) 697
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 47
Csp6I GTAC 2 cut(s) 173, 578
CspAI ACCGGT 1 cut(s) 47
CviAII CATG 3 cut(s) 347, 573, 702
CviQI GTAC 2 cut(s) 173, 578
DdeI CTNAG 1 cut(s) 414
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
EaeI YGGCCR 1 cut(s) 695
Eam1104I CTCTTC 2 cut(s) 587, 593
EarI CTCTTC 2 cut(s) 587, 593
Eco57I CTGAAG 3 cut(s) 131, 480, 574
EcoNI CCTNNNNNAGG 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 692
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 3 cut(s) 350, 576, 705
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 3 cut(s) 346, 572, 701
FblI GTMKAC 1 cut(s) 649
Fnu4HI GCNGC 7 cut(s) 24, 33, 36, 126, 398, 401, 698
Fsp4HI GCNGC 7 cut(s) 24, 33, 36, 126, 398, 401, 698
FspBI CTAG 1 cut(s) 243
GluI GCNGC 7 cut(s) 24, 33, 36, 126, 398, 401, 698
HaeIII GGCC 1 cut(s) 697
HapII CCGG 1 cut(s) 48
Hin1II CATG 3 cut(s) 350, 576, 705
HpaII CCGG 1 cut(s) 48
HphI GGTGA 2 cut(s) 402, 608
Hpy166II GTNNAC 3 cut(s) 578, 650, 663
Hpy188I TCNGA 2 cut(s) 111, 169
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 3 cut(s) 578, 650, 663
HpyAV CCTTC 4 cut(s) 106, 242, 455, 595
HpyCH4III ACNGT 5 cut(s) 550, 620, 647, 667, 678
HpyCH4IV ACGT 2 cut(s) 209, 334
HpyCH4V TGCA 8 cut(s) 26, 32, 397, 427, 497, 572, 658, 685
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 29, 32, 122, 131, 424, 697
HpyF3I CTNAG 1 cut(s) 414
HpySE526I ACGT 2 cut(s) 209, 334
Hsp92II CATG 3 cut(s) 350, 576, 705
Kzo9I GATC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 14, 559
LpnPI CCDG 7 cut(s) 61, 148, 276, 368, 413, 417, 679
Lsp1109I GCAGC 5 cut(s) 10, 44, 137, 387, 409
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 209, 334
MaeIII GTNAC 2 cut(s) 454, 672
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 3 cut(s) 53, 574, 580
MfeI CAATTG 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 5 cut(s) 27, 219, 356, 369, 505
MnlI CCTC 3 cut(s) 423, 512, 596
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 1 cut(s) 180
MspA1I CMGCKG 2 cut(s) 35, 400
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 694
MunI CAATTG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 1 cut(s) 694
MwoI GCNNNNNNNGC 7 cut(s) 23, 29, 32, 122, 131, 424, 697
NdeII GATC 1 cut(s) 157
NlaIII CATG 3 cut(s) 350, 576, 705
NlaIV GGNNCC 3 cut(s) 16, 52, 433
NspI RCATGY 1 cut(s) 576
PctI GAATGC 1 cut(s) 499
PdmI GAANNNNTTC 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 7 cut(s) 25, 34, 37, 127, 399, 402, 699
Psp6I CCWGG 1 cut(s) 692
PspGI CCWGG 1 cut(s) 692
PspN4I GGNNCC 3 cut(s) 16, 52, 433
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 2 cut(s) 174, 579
RsaNI GTAC 2 cut(s) 173, 578
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 7 cut(s) 24, 33, 36, 126, 398, 401, 698
Sau3AI GATC 1 cut(s) 157
ScrFI CCNGG 1 cut(s) 694
SduI GDGCHC 1 cut(s) 436
SetI ASST 9 cut(s) 85, 212, 295, 337, 402, 415, 466, 473, 641
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 5 cut(s) 27, 219, 356, 369, 505
SsiI CCGC 5 cut(s) 35, 141, 288, 484, 698
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 692
TaaI ACNGT 5 cut(s) 550, 620, 647, 667, 678
TaiI ACGT 2 cut(s) 212, 337
TaqI TCGA 3 cut(s) 44, 77, 630
TasI AATT 5 cut(s) 27, 219, 356, 369, 505
TatI WGTACW 1 cut(s) 577
TauI GCSGC 2 cut(s) 38, 700
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseI GCWGC 5 cut(s) 23, 32, 125, 397, 400
TspDTI ATGAA 2 cut(s) 550, 614
TspGWI ACGGA 4 cut(s) 54, 60, 190, 559
XagI CCTNNNNNAGG 1 cut(s) 246
XapI RAATTY 2 cut(s) 219, 356
XceI RCATGY 1 cut(s) 576
XmiI GTMKAC 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.