Rh5BG086000

Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016799
Physical Location & Seq
Reverse (-)
7448320 .. 7452175
3856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG086000.1

Sequence Viewer

Length: 1644 bp
ATGGCTTGTGTGATTTATGTTCCTGGAGCAGCCTTTGCAAGTTCCACCAGTACTGTCAATGCCTTATTTTCTTATTTTCTCAAGGAAAATCAGCCTAGTGAAGGTTTCTTGATGTGGAGTCTGGTAGTTGTTCTCATCACTGCAGCAATTTGGCTTGCTCTGGCTACATATTTACAGCTACCAGTCTCATCCCTGCAATCTATACAAGGTGCTTTGTTAGGAACAATACTTGTTACTCAAGGTTTTGGCTACATACCATTGTGGAATAAGAATCAGAACCACAATTTCATTGGTGGAGGACTTGTTTGGATTGTTCTTGAGTGGACTGTTGCTCCATTAATTGCCTTTCTATGCGCATTCCTCCTTTTTGCTCTCATGAAGGTTTCTCTGCTTCGCCATGAAAATGCCGAAAAAAGGATTCTTGTTTTTCTCCCAATTGACTATGGAATTTCAGCTGGACTGCTTTGCCTCTTTCTCATGTATCAGGTGTTACCACATATGATAGCTCTTTATAGTTGGGTGATGATTGTTGCAGTTACAGTGGCCACCTTGATTGGAGCTCTATTATCTTTGCTTGTTGTGGTTCCTTTGGCTATGAAAAAACTAAAAGCAGTCAAAAATTACAAGACCATGAAGAGAAACATGACAAAATCTTCAGATCATAGTAAAAGTAAATGCATAGAAAGCCAAGATCCAACATTTAATGACACAAAATCAGCTGATGATGAAGCTCAGTTTGAAGAAGCCTTGAAAGATTTCATGCAGATGAGAGTCCTTGACACGGTGTATGAAGAGGACGAGAGGAGCTGGGGTTCACCGGACACATTAGTCCAAGAACCCGAAAATAAGCTTGCTGAAGATCAATCTCCTGCTTCTCAAGATCAGTCTAATATAGACAAATCTACTTCATTTAGAAAGTTGCTTGAGTCTACACCAAATCAGTTGGTTCAAAGTCACACAAGGAACTTTCAGAAGATTGAGAAAGCAACACCAATCGGGAATGCCTTCCAGTTTCTAACAGGTTCATCAAACTCATTTTTTTCTCCTGTAATGGAATATGACAGACATACCCTCATTCGCCATGCTCTAGCCGAGAAATATGATGATGTGGAAGATTTCTTCAGTTTTCCCCATCTTATAGCTTCGTGCATCTTTGCGCTTATACAATCAACTAGTGAAGTTGCTGCTGTTGTTAGTCCATATGGAGCTATTCTTGATGTTTTTCATAATAGAGAAAAATATTCTGGAAATGGGGAACATGTGGGGTCAATCCATGTGACTTGGTGGTTCAAGGCAATTGGTGGATTTGGTGCAGCCATTGGTTTCTTTCTGTGTGGGTTTAGGGTGACTCAGTCACTGGGTGGGAAGCTGACTTATATGAGCAACTCGAGAGGGTTAGCAGCACAATTGTCAACTGTGGCAGCAATCATCATTGTCACTAAGATTAAGCTTCCTGTCTCAACCATTCATGCTTTGGTTGGATCCTTAGTAGGAGTTGGGATTGCAGATGACCCTCGGAATGTGAACTGGAAGCTCCTATTCAAGTTCATGTGTGGGTGGGTGATGACCATAGTCTTTTGTTCTGGGGTTGCTTATGTGATCTTCTCAGTGTCCATACACTCCCCAGCTTATGTAGTGCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHO4

Protein Analysis

547

Amino Acids

60.53

Weight (kDa)

6.89

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHO4 PF01384 2 - 530 3.6e-63 Phosphate transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016799)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27970
malus_domestica MD00G1008800.v1.1 MD10G1275200.v1.1
prunus_persica Prupe.4G067200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011761
rosa_laevigata RLG00000031860
rosa_roxburghii Rroxscaffold_1G00064790
rosa_rugosa Rorug04G0458700 Rorug04G0458800
rosa_samantha Rh5AG090500 Rh5BG086000 Rh5CG099800 Rh5DG086200
rosa_wichuraiana Rw5G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 827
Acc16I TGCGCA 1 cut(s) 355
AccI GTMKAC 1 cut(s) 929
AclWI GGATC 3 cut(s) 686, 1476, 1489
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 3 cut(s) 639, 876, 1105
AdeI CACNNNGTG 1 cut(s) 1361
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 3 cut(s) 101, 414, 781
AflIII ACRYGT 1 cut(s) 1258
AgsI TTSAA 5 cut(s) 740, 751, 950, 1291, 1543
AhlI ACTAGT 1 cut(s) 1172
AjnI CCWGG 1 cut(s) 22
AloI GAACNNNNNNTCC 2 cut(s) 796, 828
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 2 cut(s) 190, 1462
AlwI GGATC 3 cut(s) 686, 1476, 1489
AlwNI CAGNNNCTG 1 cut(s) 1357
Ama87I CYCGRG 1 cut(s) 1387
AoxI GGCC 1 cut(s) 543
ApeKI GCWGC 6 cut(s) 29, 143, 1184, 1313, 1400, 1421
ApoI RAATTY 1 cut(s) 447
AseI ATTAAT 1 cut(s) 338
Asp700I GAANNNNTTC 2 cut(s) 755, 1004
AspLEI GCGC 3 cut(s) 356, 1159, 1641
AsuHPI GGTGA 4 cut(s) 532, 807, 1357, 1573
AvaI CYCGRG 1 cut(s) 1387
BalI TGGCCA 1 cut(s) 545
BamHI GGATCC 1 cut(s) 1481
BanII GRGCYC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
BccI CCATC 1 cut(s) 1140
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 190, 1462
BcuI ACTAGT 1 cut(s) 1172
BfaI CTAG 3 cut(s) 96, 1088, 1173
BfmI CTRYAG 1 cut(s) 141
BisI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
BlsI GCNGC 6 cut(s) 31, 145, 1186, 1315, 1402, 1423
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 24
BmeT110I CYCGRG 1 cut(s) 1387
BmiI GGNNCC 2 cut(s) 585, 1483
BmrFI CCNGG 1 cut(s) 24
BmrI ACTGGG 1 cut(s) 1367
BmsI GCATC 1 cut(s) 1158
BmuI ACTGGG 1 cut(s) 1367
BoxI GACNNNNGTC 1 cut(s) 1571
BpmI CTGGAG 1 cut(s) 45
BpuEI CTTGAG 5 cut(s) 65, 222, 338, 861, 944
BsaJI CCNNGG 1 cut(s) 1514
BsaWI WCCGGW 1 cut(s) 817
BsaXI ACNNNNNCTCC 6 cut(s) 288, 316, 318, 346, 796, 826
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 414, 781
Bse1I ACTGG 5 cut(s) 48, 182, 1009, 1362, 1532
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 1514
BseGI GGATG 1 cut(s) 188
BseLI CCNNNNNNNGG 3 cut(s) 101, 414, 781
BseMII CTCAG 3 cut(s) 746, 1364, 1620
BseNI ACTGG 5 cut(s) 48, 182, 1009, 1362, 1532
BseRI GAGGAG 1 cut(s) 817
BseXI GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
BseYI CCCAGC 2 cut(s) 807, 1624
BsgI GTGCAG 1 cut(s) 1332
BshFI GGCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 562
BsiHKCI CYCGRG 1 cut(s) 1387
BsiSI CCGG 1 cut(s) 818
BslI CCNNNNNNNGG 3 cut(s) 101, 414, 781
BsmAI GTCTC 2 cut(s) 190, 1462
BsmI GAATGC 2 cut(s) 356, 1006
BsnI GGCC 1 cut(s) 545
BsoBI CYCGRG 1 cut(s) 1387
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
BspANI GGCC 1 cut(s) 545
BspCNI CTCAG 3 cut(s) 745, 1363, 1619
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 585, 1483
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 3 cut(s) 686, 1476, 1489
BsrI ACTGG 5 cut(s) 48, 182, 1009, 1362, 1532
BssECI CCNNGG 1 cut(s) 1514
BssMI GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 5 cut(s) 55, 328, 541, 784, 1417
Bst6I CTCTTC 2 cut(s) 629, 786
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 156, 852
BstDEI CTNAG 5 cut(s) 732, 1350, 1440, 1486, 1606
BstENI CCTNNNNNAGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 188
BstHHI GCGC 3 cut(s) 356, 1159, 1641
BstKTI GATC 6 cut(s) 661, 694, 862, 883, 1484, 1602
BstMAI GTCTC 2 cut(s) 190, 1462
BstMBI GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
BstMWI GCNNNNNNNGC 2 cut(s) 35, 684
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 1262
BstPAI GACNNNNGTC 1 cut(s) 1571
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 141
BstV1I GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
BstX2I RGATCY 2 cut(s) 691, 1481
BstYI RGATCY 2 cut(s) 691, 1481
BsuRI GGCC 1 cut(s) 545
BtsCI GGATG 1 cut(s) 188
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 4 cut(s) 138, 546, 1355, 1614
Cac8I GCNNGC 2 cut(s) 156, 852
CaiI CAGNNNCTG 1 cut(s) 1357
CciI TCATGA 1 cut(s) 375
CfoI GCGC 3 cut(s) 356, 1159, 1641
Csp6I GTAC 1 cut(s) 51
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 5 cut(s) 732, 1350, 1440, 1486, 1606
DpnI GATC 6 cut(s) 660, 693, 861, 882, 1483, 1601
DpnII GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
DraIII CACNNNGTG 1 cut(s) 1361
DrdI GACNNNNNNGTC 1 cut(s) 827
DseDI GACNNNNNNGTC 1 cut(s) 827
EaeI YGGCCR 1 cut(s) 543
Eam1104I CTCTTC 2 cut(s) 629, 786
EarI CTCTTC 2 cut(s) 629, 786
Ecl136II GAGCTC 1 cut(s) 560
Eco24I GRGCYC 1 cut(s) 562
Eco53kI GAGCTC 1 cut(s) 560
Eco57I CTGAAG 3 cut(s) 639, 876, 1105
Eco88I CYCGRG 1 cut(s) 1387
EcoICRI GAGCTC 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 22
EcoT22I ATGCAT 1 cut(s) 680
EcoT38I GRGCYC 1 cut(s) 562
FauNDI CATATG 2 cut(s) 498, 1201
FblI GTMKAC 1 cut(s) 929
Fnu4HI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
FokI GGATG 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
FspAI RTGCGCAY 1 cut(s) 355
FspBI CTAG 3 cut(s) 96, 1088, 1173
FspI TGCGCA 1 cut(s) 355
GlaI GCGC 3 cut(s) 355, 1158, 1640
GluI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
GsaI CCCAGC 2 cut(s) 811, 1628
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 1 cut(s) 545
HapII CCGG 1 cut(s) 818
HhaI GCGC 3 cut(s) 356, 1159, 1641
Hin6I GCGC 3 cut(s) 354, 1157, 1639
HinP1I GCGC 3 cut(s) 354, 1157, 1639
HincII GTYRAC 1 cut(s) 1413
HindII GTYRAC 1 cut(s) 1413
HindIII AAGCTT 2 cut(s) 848, 1448
HinfI GANTC 6 cut(s) 118, 271, 418, 771, 926, 1348
HpaII CCGG 1 cut(s) 818
HphI GGTGA 4 cut(s) 532, 807, 1357, 1573
Hpy166II GTNNAC 5 cut(s) 324, 815, 930, 1413, 1525
Hpy188I TCNGA 4 cut(s) 276, 658, 972, 1518
Hpy188III TCNNGA 8 cut(s) 109, 317, 376, 878, 997, 1214, 1245, 1389
Hpy8I GTNNAC 5 cut(s) 324, 815, 930, 1413, 1525
HpyAV CCTTC 3 cut(s) 95, 373, 1015
HpyCH4III ACNGT 5 cut(s) 55, 328, 541, 784, 1417
HpyCH4V TGCA 9 cut(s) 38, 143, 196, 533, 678, 763, 1149, 1313, 1505
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 684
HpyF3I CTNAG 5 cut(s) 732, 1350, 1440, 1486, 1606
HspAI GCGC 3 cut(s) 354, 1157, 1639
Kzo9I GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
LmnI GCTCC 6 cut(s) 26, 337, 557, 804, 1205, 1539
Lsp1109I GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
LweI GCATC 1 cut(s) 1158
MaeI CTAG 3 cut(s) 96, 1088, 1173
MaeIII GTNAC 8 cut(s) 232, 489, 535, 953, 1276, 1345, 1353, 1435
MalI GATC 6 cut(s) 660, 693, 861, 882, 1483, 1601
MboI GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
MboII GAAGA 9 cut(s) 645, 646, 752, 803, 869, 985, 1111, 1124, 1594
MfeI CAATTG 3 cut(s) 435, 1296, 1406
MflI RGATCY 2 cut(s) 691, 1481
MhlI GDGCHC 1 cut(s) 562
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 8 cut(s) 147, 283, 339, 435, 447, 619, 1296, 1406
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 4 cut(s) 127, 780, 935, 1342
MmeI TCCRAC 2 cut(s) 719, 1459
MnlI CCTC 8 cut(s) 290, 371, 479, 787, 795, 1082, 1385, 1524
Mox20I TGGCCA 1 cut(s) 545
Mph1103I ATGCAT 1 cut(s) 680
MroXI GAANNNNTTC 2 cut(s) 755, 1004
MscI TGGCCA 1 cut(s) 545
MseI TTAA 3 cut(s) 338, 702, 1446
MslI CAYNNNNRTG 2 cut(s) 402, 764
Msp20I TGGCCA 1 cut(s) 545
MspA1I CMGCKG 2 cut(s) 455, 719
MspI CCGG 1 cut(s) 818
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 3 cut(s) 435, 1296, 1406
Mva1269I GAATGC 2 cut(s) 356, 1006
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 35, 684
NdeI CATATG 2 cut(s) 498, 1201
NdeII GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
NlaIV GGNNCC 2 cut(s) 585, 1483
NmeAIII GCCGAG 1 cut(s) 1117
NmuCI GTSAC 5 cut(s) 953, 1276, 1345, 1353, 1435
NsbI TGCGCA 1 cut(s) 355
NsiI ATGCAT 1 cut(s) 680
NspI RCATGY 1 cut(s) 1262
PaeR7I CTCGAG 1 cut(s) 1387
PagI TCATGA 1 cut(s) 375
PciI ACATGT 1 cut(s) 1258
PctI GAATGC 2 cut(s) 356, 1006
PdmI GAANNNNTTC 2 cut(s) 755, 1004
PfeI GAWTC 2 cut(s) 271, 418
PflFI GACNNNGTC 1 cut(s) 1351
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 6 cut(s) 31, 145, 1186, 1315, 1402, 1423
PleI GAGTC 4 cut(s) 126, 779, 934, 1342
PpsI GAGTC 4 cut(s) 126, 779, 934, 1342
PscI ACATGT 1 cut(s) 1258
PshAI GACNNNNGTC 1 cut(s) 1571
PshBI ATTAAT 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 22
PspFI CCCAGC 2 cut(s) 807, 1624
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 2 cut(s) 585, 1483
PstI CTGCAG 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 1357
PsuI RGATCY 2 cut(s) 691, 1481
PsyI GACNNNGTC 1 cut(s) 1351
PvuII CAGCTG 2 cut(s) 455, 719
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 2 cut(s) 402, 764
SacI GAGCTC 1 cut(s) 562
SaqAI TTAA 3 cut(s) 338, 702, 1446
SatI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
Sau3AI GATC 6 cut(s) 658, 691, 859, 880, 1481, 1599
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 4 cut(s) 127, 780, 935, 1342
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 1 cut(s) 1158
SfcI CTRYAG 1 cut(s) 141
Sfr274I CTCGAG 1 cut(s) 1387
SlaI CTCGAG 1 cut(s) 1387
SmiMI CAYNNNNRTG 2 cut(s) 402, 764
SmlI CTYRAG 6 cut(s) 80, 237, 317, 876, 923, 1387
SmoI CTYRAG 6 cut(s) 80, 237, 317, 876, 923, 1387
SpeI ACTAGT 1 cut(s) 1172
Sse9I AATT 8 cut(s) 147, 283, 339, 435, 447, 619, 1296, 1406
SspI AATATT 1 cut(s) 1241
SspMI CTAG 3 cut(s) 96, 1088, 1173
SstI GAGCTC 1 cut(s) 562
StyD4I CCNGG 1 cut(s) 22
TaaI ACNGT 5 cut(s) 55, 328, 541, 784, 1417
TaqI TCGA 1 cut(s) 1388
TasI AATT 8 cut(s) 147, 283, 339, 435, 447, 619, 1296, 1406
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 2 cut(s) 271, 418
Tru1I TTAA 3 cut(s) 338, 702, 1446
Tru9I TTAA 3 cut(s) 338, 702, 1446
TscAI CASTG 4 cut(s) 145, 546, 1362, 1614
TseFI GTSAC 5 cut(s) 953, 1276, 1345, 1353, 1435
TseI GCWGC 6 cut(s) 29, 143, 1184, 1313, 1400, 1421
Tsp45I GTSAC 5 cut(s) 953, 1276, 1345, 1353, 1435
TspRI CASTG 4 cut(s) 145, 546, 1362, 1614
Tth111I GACNNNGTC 1 cut(s) 1351
VspI ATTAAT 1 cut(s) 338
XagI CCTNNNNNAGG 1 cut(s) 99
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 1262
XcmI CCANNNNNNNNNTGG 2 cut(s) 287, 1471
XhoI CTCGAG 1 cut(s) 1387
XmiI GTMKAC 1 cut(s) 929
XmnI GAANNNNTTC 2 cut(s) 755, 1004
XspI CTAG 3 cut(s) 96, 1088, 1173
ZrmI AGTACT 1 cut(s) 52
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.