Rroxscaffold_1G00064790

Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016799
Physical Location & Seq
Forward (+)
86596012 .. 86599948
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064790.1

Sequence Viewer

Length: 1494 bp
ATGGCTTGTGTGATTTATGTTCCTGGAGCAGCCTTTGCAAGTTCCACCAGTACTGTCAATGCCTTATTTTCTTATTTTCTCAAGGAAAATCAGCCTAGTGAAGGTTTCTTGATGTGGAGTCTGGTAGTTATTCTCATCACTGCAGCAATTTGGCTTGCTCTGGCTACATATTTACAGCTACCAGTCTCATCCCTGCAATCTATACAAGGTGCTTTGTTAGGAACAATACTTGTTACTCAAGGTTTTGGCTACATACCATTGTGGAATAAGAATCAGAACCACAATTTCATTGGTGGAGGACTTGTTTGGATTGTTCTTGAGTGGACTGTTGCTCCATTAATTGCCTTTCTATGCGCATTCCTCCTTTTTGCTCTCATGAAGGTTTCTCTGCTTCGCCATGAAAATGCCGAAAAAAGGATTCTTGTTTTTCTCCCGATTGACTATGGAATTTCAGCTGGACTGCTTTGCCTCTTTCTCATGTATCAGGTGTTACCACATATGATAACTCTTTATAGTTGGGTGATGATTGTTGCAGTTGCAGTGGCCACCTTGATTGGAGCTCTATTATCTTTGCTTGTTGTGGTTCCTTTGGCCATGAAAAAACTAAAAGCAGTCAAAAATTACAAGACCATGAAGAGAAACATGACAAAATCTTCAGATCATAGTAAAAGTAAATGCATAGAAAGCCAAGATCCAACATTTAATGACACAAAATCAGCTGATGATGAAGCTCAGTTTGAAGAAGCCTTGAAAGATTTCATGCAGATGAGAGTCCTTGACACGGTGTATGAAGAGGACGAGAGGAGCTGGGGTTCACCGGACACATTAGTCCAAGAACCCGAAAATAAGCTTGCTGAAGATCAATCTCCTGCTTCTCAAGATCAGTCTAATATAGACAAATCTACTTCATTTAGAAAGTTGCTTGAGTCTACACCAAATCAGTTGGTTCAAAGTCACACAAGGAACTTTCAGAAGATTGAGAAAGCAACACCAATCTGGAATGCCTTCCAGTTTCTAACAGGTTCATCAAACTCATTTTTTTCTCCTGTAATGGAATATGACAGGCATACCCTCATTCGCCATGCTCTAGCCGAGAAATATGATGATGTGGAAGATTTCTTCAGTTTTCCCCATCTTATAGCTTCGTGCATCTTTGCGCTTATACAATCAACTAGTGAAGTTGCTGCTGTTGTTAGTCCATATGGAGCTATTCTTGATGTTTTTCATAATAGAGAAAAATATTCTGGAAATGGGGAACATGTGGGATCAATCCATGTGACTTGGTGGTTCAAGGCAATTGGTGGATTTGGTGCAGCCATTGGTTTCTTTCTGTGTGGGTTTAGGGTGACTCAGTCACTGGGTGGGAGGCTGACTTATATGAGCAACTCAAGAGGGTTAGCAGCACAATTGTCAACTGTGGCAGCAATCATCATTGTCACACTAAGATTAAGCTTCCTGTCTCAACCATTCATGCTTTGGTTGGATCCTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHO4

Protein Analysis

497

Amino Acids

55.5

Weight (kDa)

6.14

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHO4 PF01384 2 - 480 5.7e-45 Phosphate transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016799)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27970
malus_domestica MD00G1008800.v1.1 MD10G1275200.v1.1
prunus_persica Prupe.4G067200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0011761
rosa_laevigata RLG00000031860
rosa_roxburghii Rroxscaffold_1G00064790
rosa_rugosa Rorug04G0458700 Rorug04G0458800
rosa_samantha Rh5AG090500 Rh5BG086000 Rh5CG099800 Rh5DG086200
rosa_wichuraiana Rw5G007900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 827
Acc16I TGCGCA 1 cut(s) 355
AccI GTMKAC 1 cut(s) 929
AclWI GGATC 4 cut(s) 686, 1273, 1478, 1491
AcoI YGGCCR 2 cut(s) 543, 591
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 3 cut(s) 639, 876, 1105
AdeI CACNNNGTG 1 cut(s) 1361
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 3 cut(s) 101, 414, 781
AflIII ACRYGT 1 cut(s) 1258
AgsI TTSAA 4 cut(s) 740, 751, 950, 1291
AhlI ACTAGT 1 cut(s) 1172
AjnI CCWGG 1 cut(s) 22
AloI GAACNNNNNNTCC 2 cut(s) 796, 828
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 2 cut(s) 190, 1464
AlwI GGATC 4 cut(s) 686, 1273, 1478, 1491
AlwNI CAGNNNCTG 1 cut(s) 1357
AoxI GGCC 2 cut(s) 543, 591
ApeKI GCWGC 6 cut(s) 29, 143, 1184, 1313, 1400, 1421
ApoI RAATTY 1 cut(s) 447
AseI ATTAAT 1 cut(s) 338
Asp700I GAANNNNTTC 2 cut(s) 755, 1004
AspLEI GCGC 2 cut(s) 356, 1159
AsuHPI GGTGA 3 cut(s) 532, 807, 1357
BalI TGGCCA 2 cut(s) 545, 593
BamHI GGATCC 1 cut(s) 1483
BanII GRGCYC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
BccI CCATC 1 cut(s) 1140
BciT130I CCWGG 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 190, 1464
BcuI ACTAGT 1 cut(s) 1172
BfaI CTAG 3 cut(s) 96, 1088, 1173
BfmI CTRYAG 1 cut(s) 141
BisI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
BlsI GCNGC 6 cut(s) 31, 145, 1186, 1315, 1402, 1423
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 24
BmiI GGNNCC 2 cut(s) 585, 1485
BmrFI CCNGG 1 cut(s) 24
BmrI ACTGGG 1 cut(s) 1367
BmsI GCATC 1 cut(s) 1158
BmuI ACTGGG 1 cut(s) 1367
BpmI CTGGAG 1 cut(s) 45
BpuEI CTTGAG 6 cut(s) 65, 222, 338, 861, 944, 1372
BsaJI CCNNGG 1 cut(s) 1487
BsaWI WCCGGW 1 cut(s) 817
BsaXI ACNNNNNCTCC 6 cut(s) 288, 316, 318, 346, 796, 826
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 414, 781
Bse1I ACTGG 4 cut(s) 48, 182, 1009, 1362
BseBI CCWGG 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 1487
BseGI GGATG 1 cut(s) 188
BseLI CCNNNNNNNGG 3 cut(s) 101, 414, 781
BseMII CTCAG 2 cut(s) 746, 1364
BseNI ACTGG 4 cut(s) 48, 182, 1009, 1362
BseRI GAGGAG 1 cut(s) 817
BseXI GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
BseYI CCCAGC 1 cut(s) 807
BsgI GTGCAG 1 cut(s) 1332
BshFI GGCC 2 cut(s) 545, 593
BsiHKAI GWGCWC 1 cut(s) 562
BsiSI CCGG 1 cut(s) 818
BslI CCNNNNNNNGG 3 cut(s) 101, 414, 781
BsmAI GTCTC 2 cut(s) 190, 1464
BsmI GAATGC 2 cut(s) 356, 1006
BsnI GGCC 2 cut(s) 545, 593
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
BspANI GGCC 2 cut(s) 545, 593
BspCNI CTCAG 2 cut(s) 745, 1363
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 585, 1485
BspMAI CTGCAG 1 cut(s) 145
BspPI GGATC 4 cut(s) 686, 1273, 1478, 1491
BsrI ACTGG 4 cut(s) 48, 182, 1009, 1362
BssECI CCNNGG 1 cut(s) 1487
BssMI GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
BssT1I CCWWGG 1 cut(s) 1487
Bst2UI CCWGG 1 cut(s) 24
Bst4CI ACNGT 4 cut(s) 55, 328, 784, 1417
Bst6I CTCTTC 2 cut(s) 629, 786
BstAPI GCANNNNNTGC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 156, 852
BstDEI CTNAG 3 cut(s) 732, 1350, 1442
BstENI CCTNNNNNAGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 188
BstHHI GCGC 2 cut(s) 356, 1159
BstKTI GATC 6 cut(s) 661, 694, 862, 883, 1268, 1486
BstMAI GTCTC 2 cut(s) 190, 1464
BstMBI GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
BstMWI GCNNNNNNNGC 2 cut(s) 35, 684
BstNI CCWGG 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 1262
BstSCI CCNGG 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 141
BstV1I GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
BstX2I RGATCY 2 cut(s) 691, 1483
BstYI RGATCY 2 cut(s) 691, 1483
BsuRI GGCC 2 cut(s) 545, 593
BtsCI GGATG 1 cut(s) 188
BtsI GCAGTG 2 cut(s) 138, 546
BtsIMutI CAGTG 3 cut(s) 138, 546, 1355
Cac8I GCNNGC 2 cut(s) 156, 852
CaiI CAGNNNCTG 1 cut(s) 1357
CciI TCATGA 1 cut(s) 375
CfoI GCGC 2 cut(s) 356, 1159
Csp6I GTAC 1 cut(s) 51
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 3 cut(s) 732, 1350, 1442
DpnI GATC 6 cut(s) 660, 693, 861, 882, 1267, 1485
DpnII GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
DraIII CACNNNGTG 1 cut(s) 1361
DrdI GACNNNNNNGTC 1 cut(s) 827
DseDI GACNNNNNNGTC 1 cut(s) 827
EaeI YGGCCR 2 cut(s) 543, 591
Eam1104I CTCTTC 2 cut(s) 629, 786
EarI CTCTTC 2 cut(s) 629, 786
Ecl136II GAGCTC 1 cut(s) 560
Eco130I CCWWGG 1 cut(s) 1487
Eco24I GRGCYC 1 cut(s) 562
Eco53kI GAGCTC 1 cut(s) 560
Eco57I CTGAAG 3 cut(s) 639, 876, 1105
EcoICRI GAGCTC 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 1487
EcoT22I ATGCAT 1 cut(s) 680
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 1 cut(s) 1487
FauNDI CATATG 2 cut(s) 498, 1201
FblI GTMKAC 1 cut(s) 929
Fnu4HI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
FokI GGATG 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
FspAI RTGCGCAY 1 cut(s) 355
FspBI CTAG 3 cut(s) 96, 1088, 1173
FspI TGCGCA 1 cut(s) 355
GlaI GCGC 2 cut(s) 355, 1158
GluI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
GsaI CCCAGC 1 cut(s) 811
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 2 cut(s) 545, 593
HapII CCGG 1 cut(s) 818
HhaI GCGC 2 cut(s) 356, 1159
Hin6I GCGC 2 cut(s) 354, 1157
HinP1I GCGC 2 cut(s) 354, 1157
HincII GTYRAC 1 cut(s) 1413
HindII GTYRAC 1 cut(s) 1413
HindIII AAGCTT 2 cut(s) 848, 1450
HinfI GANTC 6 cut(s) 118, 271, 418, 771, 926, 1348
HpaII CCGG 1 cut(s) 818
HphI GGTGA 3 cut(s) 532, 807, 1357
Hpy166II GTNNAC 4 cut(s) 324, 815, 930, 1413
Hpy188I TCNGA 3 cut(s) 276, 658, 972
Hpy188III TCNNGA 9 cut(s) 109, 317, 376, 433, 878, 997, 1214, 1245, 1389
Hpy8I GTNNAC 4 cut(s) 324, 815, 930, 1413
HpyAV CCTTC 3 cut(s) 95, 373, 1015
HpyCH4III ACNGT 4 cut(s) 55, 328, 784, 1417
HpyCH4V TGCA 9 cut(s) 38, 143, 196, 533, 539, 678, 763, 1149, 1313
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 684
HpyF3I CTNAG 3 cut(s) 732, 1350, 1442
HspAI GCGC 2 cut(s) 354, 1157
Kzo9I GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
LmnI GCTCC 5 cut(s) 26, 337, 557, 804, 1205
Lsp1109I GCAGC 6 cut(s) 41, 155, 1171, 1325, 1412, 1433
LweI GCATC 1 cut(s) 1158
MaeI CTAG 3 cut(s) 96, 1088, 1173
MaeIII GTNAC 7 cut(s) 232, 489, 953, 1276, 1345, 1353, 1435
MalI GATC 6 cut(s) 660, 693, 861, 882, 1267, 1485
MboI GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
MboII GAAGA 8 cut(s) 645, 646, 752, 803, 869, 985, 1111, 1124
MfeI CAATTG 2 cut(s) 1296, 1406
MflI RGATCY 2 cut(s) 691, 1483
MhlI GDGCHC 1 cut(s) 562
MlsI TGGCCA 2 cut(s) 545, 593
MluCI AATT 7 cut(s) 147, 283, 339, 447, 619, 1296, 1406
MluNI TGGCCA 2 cut(s) 545, 593
MlyI GAGTC 4 cut(s) 127, 780, 935, 1342
MmeI TCCRAC 2 cut(s) 719, 1461
MnlI CCTC 8 cut(s) 290, 371, 479, 787, 795, 1082, 1359, 1385
Mox20I TGGCCA 2 cut(s) 545, 593
Mph1103I ATGCAT 1 cut(s) 680
MroXI GAANNNNTTC 2 cut(s) 755, 1004
MscI TGGCCA 2 cut(s) 545, 593
MseI TTAA 3 cut(s) 338, 702, 1448
MslI CAYNNNNRTG 2 cut(s) 402, 764
Msp20I TGGCCA 2 cut(s) 545, 593
MspA1I CMGCKG 2 cut(s) 455, 719
MspI CCGG 1 cut(s) 818
MspR9I CCNGG 1 cut(s) 24
MunI CAATTG 2 cut(s) 1296, 1406
Mva1269I GAATGC 2 cut(s) 356, 1006
MvaI CCWGG 1 cut(s) 24
MwoI GCNNNNNNNGC 2 cut(s) 35, 684
NdeI CATATG 2 cut(s) 498, 1201
NdeII GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
NlaIV GGNNCC 2 cut(s) 585, 1485
NmeAIII GCCGAG 1 cut(s) 1117
NmuCI GTSAC 5 cut(s) 953, 1276, 1345, 1353, 1435
NsbI TGCGCA 1 cut(s) 355
NsiI ATGCAT 1 cut(s) 680
NspI RCATGY 1 cut(s) 1262
PagI TCATGA 1 cut(s) 375
PciI ACATGT 1 cut(s) 1258
PctI GAATGC 2 cut(s) 356, 1006
PdmI GAANNNNTTC 2 cut(s) 755, 1004
PfeI GAWTC 2 cut(s) 271, 418
PflFI GACNNNGTC 1 cut(s) 1351
PfoI TCCNGGA 1 cut(s) 22
PkrI GCNGC 6 cut(s) 31, 145, 1186, 1315, 1402, 1423
PleI GAGTC 4 cut(s) 126, 779, 934, 1342
PpsI GAGTC 4 cut(s) 126, 779, 934, 1342
PscI ACATGT 1 cut(s) 1258
PshBI ATTAAT 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 22
PspFI CCCAGC 1 cut(s) 807
PspGI CCWGG 1 cut(s) 22
PspN4I GGNNCC 2 cut(s) 585, 1485
PstI CTGCAG 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 1357
PsuI RGATCY 2 cut(s) 691, 1483
PsyI GACNNNGTC 1 cut(s) 1351
PvuII CAGCTG 2 cut(s) 455, 719
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 2 cut(s) 402, 764
SacI GAGCTC 1 cut(s) 562
SaqAI TTAA 3 cut(s) 338, 702, 1448
SatI GCNGC 6 cut(s) 30, 144, 1185, 1314, 1401, 1422
Sau3AI GATC 6 cut(s) 658, 691, 859, 880, 1265, 1483
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 4 cut(s) 127, 780, 935, 1342
ScrFI CCNGG 1 cut(s) 24
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 1 cut(s) 1158
SfcI CTRYAG 1 cut(s) 141
SmiMI CAYNNNNRTG 2 cut(s) 402, 764
SmlI CTYRAG 6 cut(s) 80, 237, 317, 876, 923, 1387
SmoI CTYRAG 6 cut(s) 80, 237, 317, 876, 923, 1387
SpeI ACTAGT 1 cut(s) 1172
Sse9I AATT 7 cut(s) 147, 283, 339, 447, 619, 1296, 1406
SspI AATATT 1 cut(s) 1241
SspMI CTAG 3 cut(s) 96, 1088, 1173
SstI GAGCTC 1 cut(s) 562
StyD4I CCNGG 1 cut(s) 22
StyI CCWWGG 1 cut(s) 1487
TaaI ACNGT 4 cut(s) 55, 328, 784, 1417
TasI AATT 7 cut(s) 147, 283, 339, 447, 619, 1296, 1406
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 2 cut(s) 271, 418
Tru1I TTAA 3 cut(s) 338, 702, 1448
Tru9I TTAA 3 cut(s) 338, 702, 1448
TscAI CASTG 3 cut(s) 145, 546, 1362
TseFI GTSAC 5 cut(s) 953, 1276, 1345, 1353, 1435
TseI GCWGC 6 cut(s) 29, 143, 1184, 1313, 1400, 1421
Tsp45I GTSAC 5 cut(s) 953, 1276, 1345, 1353, 1435
TspRI CASTG 3 cut(s) 145, 546, 1362
Tth111I GACNNNGTC 1 cut(s) 1351
VspI ATTAAT 1 cut(s) 338
XagI CCTNNNNNAGG 1 cut(s) 99
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 1262
XcmI CCANNNNNNNNNTGG 2 cut(s) 287, 1473
XmiI GTMKAC 1 cut(s) 929
XmnI GAANNNNTTC 2 cut(s) 755, 1004
XspI CTAG 3 cut(s) 96, 1088, 1173
ZrmI AGTACT 1 cut(s) 52
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.