MD00G1023000.v1.1

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012884
Physical Location & Seq
Reverse (-)
3868404 .. 3869714
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1023000.v1.1.491

Sequence Viewer

Length: 516 bp
ATGATGAAGGCTGTGAGTGTTCTTGTTCATTTGGGCTTCCTTTTCCTCTTCCTCTTCACCTTCGCACAAGGCAGCAGCAGATCAATCATTCCACAGCCCCAACCTCTCACATCTTTCAATATCAACAAAACCCAGAACGCAGGGAGCTGTTCTTACACTGTATCAATACAGACCAGCTGTTCTTCGCCCCGCTATACACGAGATCAGATCAGTATTGCCTTTGGTGATGCTTATGGCAATCAGGTGTACGCTCCTAGAATCGACGATCCGTATTCGAGGACATTCGAGAGCTGCTCGACGGATACGTTTAAGATTTCGGGGCCATGTACATACCAGATCTGTTATGTGTATCTGTACCGGAGCGGATACGATGGGTGGAAAACAGAGGCAGTGACAATCTATGGCCATTACACCAAGGCTGTTACGTTTTACTACAACTCTTTTATTCCAAACGGTGTTTGGTATGGATTTAATCAGTGTAACGGTGCTGCAGCATCCTCCACTTGGAGCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATS3

Protein Analysis

172

Amino Acids

19.19

Weight (kDa)

8.5

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 43 - 160 1.3e-52 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41475
fragaria_vesca FvH4_6g29960 FvH4_6g29960
malus_domestica MD00G1023000.v1.1 MD02G1031500.v1.1
prunus_persica Prupe.3G084200_v2.0.a1 Prupe.7G243200_v2.0.a1
pyrus_communis pycom02g02630 pycom17g21450
rosa_chinensis RchiOBHm_Chr2g0135961
rosa_laevigata RLG00000019492
rosa_multiflora Rmu_ssc0000343.1_g000020
rosa_roxburghii Rroxscaffold_2G00108740
rosa_samantha Rh2AG378900 Rh2BG385400 Rh2CG364500 Rh2DG401700
rosa_wichuraiana Rw2G030870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 363
AciI CCGC 2 cut(s) 190, 363
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 403
AfaI GTAC 3 cut(s) 248, 328, 356
AfiI CCNNNNNNNGG 1 cut(s) 504
AgsI TTSAA 1 cut(s) 118
AluBI AGCT 3 cut(s) 147, 177, 291
AluI AGCT 3 cut(s) 147, 177, 291
AlwI GGATC 1 cut(s) 260
AoxI GGCC 2 cut(s) 320, 403
ApeKI GCWGC 5 cut(s) 72, 75, 291, 488, 491
AspS9I GGNCC 1 cut(s) 320
AsuHPI GGTGA 2 cut(s) 49, 236
BalI TGGCCA 1 cut(s) 405
BauI CACGAG 1 cut(s) 198
BbvI GCAGC 5 cut(s) 84, 87, 278, 475, 503
BccI CCATC 1 cut(s) 365
BciVI GTATCC 2 cut(s) 295, 359
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 489
BfuI GTATCC 2 cut(s) 295, 359
BglII AGATCT 1 cut(s) 336
BisI GCNGC 5 cut(s) 73, 76, 292, 489, 492
BlsI GCNGC 5 cut(s) 74, 77, 293, 490, 493
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 321
BmsI GCATC 2 cut(s) 217, 503
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BsaJI CCNNGG 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 357
Bsc4I CCNNNNNNNGG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 1 cut(s) 494
BseLI CCNNNNNNNGG 1 cut(s) 504
BseXI GCAGC 5 cut(s) 84, 87, 278, 475, 503
BshFI GGCC 2 cut(s) 322, 405
BsiSI CCGG 1 cut(s) 358
BslI CCNNNNNNNGG 1 cut(s) 504
BsnI GGCC 2 cut(s) 322, 405
Bsp1407I TGTACA 1 cut(s) 326
Bsp143I GATC 5 cut(s) 80, 202, 207, 265, 336
BspACI CCGC 2 cut(s) 190, 363
BspANI GGCC 2 cut(s) 322, 405
BspLI GGNNCC 1 cut(s) 321
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 363
BsrGI TGTACA 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 5 cut(s) 80, 202, 207, 265, 336
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 198
Bst4CI ACNGT 3 cut(s) 160, 455, 485
Bst6I CTCTTC 2 cut(s) 53, 59
BstAUI TGTACA 1 cut(s) 326
BstF5I GGATG 1 cut(s) 494
BstKTI GATC 5 cut(s) 83, 205, 210, 268, 339
BstMBI GATC 5 cut(s) 80, 202, 207, 265, 336
BstNSI RCATGY 1 cut(s) 514
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 5 cut(s) 84, 87, 278, 475, 503
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
BsuI GTATCC 2 cut(s) 295, 359
BsuRI GGCC 2 cut(s) 322, 405
BtsCI GGATG 1 cut(s) 494
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 3 cut(s) 156, 396, 482
Cfr13I GGNCC 1 cut(s) 320
Csp6I GTAC 3 cut(s) 247, 327, 355
CviAII CATG 2 cut(s) 324, 511
CviJI RGCY 9 cut(s) 11, 36, 97, 147, 177, 291, 322, 405, 419
CviKI_1 RGCY 9 cut(s) 11, 36, 97, 147, 177, 291, 322, 405, 419
CviQI GTAC 3 cut(s) 247, 327, 355
DpnI GATC 5 cut(s) 82, 204, 209, 267, 338
DpnII GATC 5 cut(s) 80, 202, 207, 265, 336
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 2 cut(s) 53, 59
EarI CTCTTC 2 cut(s) 53, 59
Eco130I CCWWGG 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 2 cut(s) 327, 514
FaiI YATR 8 cut(s) 195, 234, 325, 331, 345, 402, 465, 512
FatI CATG 2 cut(s) 323, 510
FauI CCCGC 1 cut(s) 197
Fnu4HI GCNGC 5 cut(s) 73, 76, 292, 489, 492
FokI GGATG 1 cut(s) 481
Fsp4HI GCNGC 5 cut(s) 73, 76, 292, 489, 492
FspBI CTAG 1 cut(s) 255
GluI GCNGC 5 cut(s) 73, 76, 292, 489, 492
HaeIII GGCC 2 cut(s) 322, 405
HapII CCGG 1 cut(s) 358
Hin1II CATG 2 cut(s) 327, 514
HinfI GANTC 1 cut(s) 258
HpaII CCGG 1 cut(s) 358
HphI GGTGA 2 cut(s) 49, 236
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 1 cut(s) 286
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 2 cut(s) 266, 301
HpyAV CCTTC 1 cut(s) 70
HpyCH4III ACNGT 3 cut(s) 160, 455, 485
HpyCH4IV ACGT 2 cut(s) 305, 425
HpyCH4V TGCA 1 cut(s) 491
HpySE526I ACGT 2 cut(s) 305, 425
Hsp92II CATG 2 cut(s) 327, 514
Kzo9I GATC 5 cut(s) 80, 202, 207, 265, 336
LmnI GCTCC 4 cut(s) 144, 256, 360, 507
LpnPI CCDG 6 cut(s) 126, 146, 187, 227, 347, 371
Lsp1109I GCAGC 5 cut(s) 84, 87, 278, 475, 503
LweI GCATC 2 cut(s) 217, 503
MaeI CTAG 1 cut(s) 255
MaeII ACGT 2 cut(s) 305, 425
MaeIII GTNAC 3 cut(s) 391, 421, 479
MalI GATC 5 cut(s) 82, 204, 209, 267, 338
MbiI CCGCTC 1 cut(s) 363
MboI GATC 5 cut(s) 80, 202, 207, 265, 336
MboII GAAGA 3 cut(s) 40, 46, 174
MflI RGATCY 1 cut(s) 336
MlsI TGGCCA 1 cut(s) 405
MluNI TGGCCA 1 cut(s) 405
MnlI CCTC 6 cut(s) 56, 62, 114, 270, 379, 508
Mox20I TGGCCA 1 cut(s) 405
MscI TGGCCA 1 cut(s) 405
MseI TTAA 2 cut(s) 309, 471
Msp20I TGGCCA 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 177
MspI CCGG 1 cut(s) 358
NdeII GATC 5 cut(s) 80, 202, 207, 265, 336
NlaIII CATG 2 cut(s) 327, 514
NlaIV GGNNCC 1 cut(s) 321
NmuCI GTSAC 1 cut(s) 391
NspI RCATGY 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PfeI GAWTC 1 cut(s) 258
PkrI GCNGC 5 cut(s) 74, 77, 293, 490, 493
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 177
RsaI GTAC 3 cut(s) 248, 328, 356
RsaNI GTAC 3 cut(s) 247, 327, 355
SaqAI TTAA 2 cut(s) 309, 471
SatI GCNGC 5 cut(s) 73, 76, 292, 489, 492
Sau3AI GATC 5 cut(s) 80, 202, 207, 265, 336
Sau96I GGNCC 1 cut(s) 320
SetI ASST 8 cut(s) 62, 106, 149, 179, 246, 293, 308, 428
SfaNI GCATC 2 cut(s) 217, 503
SfcI CTRYAG 1 cut(s) 489
SsiI CCGC 2 cut(s) 190, 363
SspMI CTAG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 3 cut(s) 160, 455, 485
TaiI ACGT 2 cut(s) 308, 428
TaqI TCGA 4 cut(s) 261, 275, 285, 296
TatI WGTACW 1 cut(s) 326
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 2 cut(s) 309, 471
Tru9I TTAA 2 cut(s) 309, 471
TscAI CASTG 3 cut(s) 163, 396, 482
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 5 cut(s) 72, 75, 291, 488, 491
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 17, 20
TspGWI ACGGA 2 cut(s) 258, 314
TspRI CASTG 3 cut(s) 163, 396, 482
XceI RCATGY 1 cut(s) 514
XcmI CCANNNNNNNNNTGG 1 cut(s) 456
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.