Rh2AG378900

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012884
Physical Location & Seq
Forward (+)
56737942 .. 56745104
7163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG378900.1

Sequence Viewer

Length: 972 bp
ATGATTATCACCATATGTCCTCTATGCAGAATTTGCTACAAGTTGATCACAGACACCAACAACAAAGGAATGAGCAGATCACAAGGGCCTTCAGCCCTTAAACGGAAGCGCTTGGATTCGCAATCTGGATCTTTGACAGAGCATGAAAGCAAACTCTATGATGCCATCCGCAGCAAGACAGATATGGGCATGTCTGTACAAGACTTGAGGCGAGAAACAGGACTCCAGAACAATTTATTTACAAAGGCCATCAAAACACTTCAAGCCAAACAACTGATAAAAGAGGTTAAAAATTTTGAAAACAAGACGAGGAAACATTACATGGCTACAGAGTTTGAGCCCTCGAAGGAGATAACTGGTGGTGCTTGGTATGATAATGGACAGTTTGATACCGAGTTTATAAAGGAATTGAAGCAGATTCTGGATGCTTTGGTTTTGGACAATGAGGTCCTGAAGGTGGTTAGGACAACAACAATGAAGGCCGCTGTGACTTTCTTGCTCCATTTTGCCTTCATCTTCAGCACCATACAAGCAAGATCAATCATTCCACAGCCCAAAGCTCTCAGATCTTTCAAGGTCAACAATACCCAGAATGTAGGAAGTTGTTCTTACTCGGTTTCCATAACGACCAGCTGTTCTTCACCCCGATATACTCGAGATCAGATCAGTATTGCATTTGGTGATGCTTATGGCTATCAGGTGTATGCACCTAGAATAGACAATCCACGTTCGAGGGCATTCGAGAGTTGCTCCACCGACACATTTCAGATTTCCGGGCCATGCACGTACCAGATTTGTTATGTGTATCTCTATAGGAGTGGATATGATGGGTGGTTAGTAGACACAGTGACAATCTATGGTCATAATACAAAAGCTGTTACATTCTATTATAACGCTTATATTCCCAGTGATGTTTGGTTTGGATTTAATCTCTGTCATGGTGCTTCAGCATCATCCACTTCAAGCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATS3

Protein Analysis

323

Amino Acids

36.53

Weight (kDa)

9.07

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpc34 PF05158 41 - 157 3.7e-30 RNA polymerase Rpc34 subunit
ATS3 PF06232 196 - 312 1.2e-48 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41475
fragaria_vesca FvH4_6g29960 FvH4_6g29960
malus_domestica MD00G1023000.v1.1 MD02G1031500.v1.1
prunus_persica Prupe.3G084200_v2.0.a1 Prupe.7G243200_v2.0.a1
pyrus_communis pycom02g02630 pycom17g21450
rosa_chinensis RchiOBHm_Chr2g0135961
rosa_laevigata RLG00000019492
rosa_multiflora Rmu_ssc0000343.1_g000020
rosa_roxburghii Rroxscaffold_2G00108740
rosa_samantha Rh2AG378900 Rh2BG385400 Rh2CG364500 Rh2DG401700
rosa_wichuraiana Rw2G030870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 401, 891
AasI GACNNNNNNGTC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 840
AciI CCGC 2 cut(s) 169, 483
AclWI GGATC 1 cut(s) 136
AcsI RAATTY 2 cut(s) 30, 292
AcuI CTGAAG 4 cut(s) 75, 473, 502, 930
AfaI GTAC 2 cut(s) 198, 788
AfeI AGCGCT 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 102, 457
AgsI TTSAA 5 cut(s) 263, 299, 412, 574, 963
AluBI AGCT 3 cut(s) 560, 633, 875
AluI AGCT 3 cut(s) 560, 633, 875
AlwI GGATC 1 cut(s) 136
AlwNI CAGNNNCTG 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 654
Aor51HI AGCGCT 1 cut(s) 110
AoxI GGCC 4 cut(s) 86, 246, 480, 776
ApeKI GCWGC 1 cut(s) 171
ApoI RAATTY 2 cut(s) 30, 292
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 3 cut(s) 86, 448, 776
AsuC2I CCSGG 1 cut(s) 775
AsuHPI GGTGA 2 cut(s) 633, 692
AvaI CYCGRG 1 cut(s) 654
AvaII GGWCC 1 cut(s) 448
BanII GRGCYC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 183
BccI CCATC 3 cut(s) 173, 257, 821
BclI TGATCA 1 cut(s) 45
BcnI CCSGG 1 cut(s) 775
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 2 cut(s) 327, 811
BfoI RGCGCY 1 cut(s) 112
BglII AGATCT 1 cut(s) 566
BisI GCNGC 2 cut(s) 172, 483
BlsI GCNGC 2 cut(s) 173, 484
Bme1390I CCNGG 1 cut(s) 775
Bme18I GGWCC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 654
BmgT120I GGNCC 3 cut(s) 86, 448, 776
BmrFI CCNGG 1 cut(s) 775
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 4 cut(s) 151, 415, 673, 959
BmuI ACTGGG 1 cut(s) 900
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 226
BpuMI CCSGG 1 cut(s) 775
BsaAI YACGTR 1 cut(s) 786
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 457
Bse1I ACTGG 2 cut(s) 361, 906
BseGI GGATG 3 cut(s) 165, 430, 953
BseLI CCNNNNNNNGG 2 cut(s) 102, 457
BseMII CTCAG 1 cut(s) 577
BseNI ACTGG 2 cut(s) 361, 906
BseXI GCAGC 1 cut(s) 183
BshFI GGCC 4 cut(s) 88, 248, 482, 778
BsiHKCI CYCGRG 1 cut(s) 654
BsiSI CCGG 1 cut(s) 774
BslI CCNNNNNNNGG 2 cut(s) 102, 457
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 4 cut(s) 88, 248, 482, 778
BsoBI CYCGRG 1 cut(s) 654
Bsp1286I GDGCHC 1 cut(s) 342
Bsp1407I TGTACA 1 cut(s) 196
Bsp143I GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
BspACI CCGC 2 cut(s) 169, 483
BspANI GGCC 4 cut(s) 88, 248, 482, 778
BspCNI CTCAG 1 cut(s) 576
BspPI GGATC 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 196
BsrI ACTGG 2 cut(s) 361, 906
BssMI GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
Bst4CI ACNGT 2 cut(s) 384, 847
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 196
BstBAI YACGTR 1 cut(s) 786
BstDEI CTNAG 1 cut(s) 563
BstF5I GGATG 3 cut(s) 165, 430, 953
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 7 cut(s) 48, 80, 131, 539, 569, 661, 666
BstMBI GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNSI RCATGY 2 cut(s) 193, 970
BstSCI CCNGG 1 cut(s) 773
BstSFI CTRYAG 2 cut(s) 327, 811
BstV1I GCAGC 1 cut(s) 183
BstX2I RGATCY 2 cut(s) 128, 566
BstYI RGATCY 2 cut(s) 128, 566
BsuRI GGCC 4 cut(s) 88, 248, 482, 778
BtsCI GGATG 3 cut(s) 165, 430, 953
BtsIMutI CAGTG 2 cut(s) 852, 913
CaiI CAGNNNCTG 1 cut(s) 421
CfoI GCGC 1 cut(s) 111
Cfr13I GGNCC 3 cut(s) 86, 448, 776
Csp6I GTAC 2 cut(s) 197, 787
CviAII CATG 6 cut(s) 143, 190, 322, 780, 938, 967
CviQI GTAC 2 cut(s) 197, 787
DdeI CTNAG 1 cut(s) 563
DpnI GATC 7 cut(s) 47, 79, 130, 538, 568, 660, 665
DpnII GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 448
Eco47III AGCGCT 1 cut(s) 110
Eco57I CTGAAG 4 cut(s) 75, 473, 502, 930
Eco88I CYCGRG 1 cut(s) 654
EcoO109I RGGNCCY 2 cut(s) 86, 448
EcoT38I GRGCYC 1 cut(s) 342
FaeI CATG 6 cut(s) 146, 193, 325, 783, 941, 970
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FatI CATG 6 cut(s) 142, 189, 321, 779, 937, 966
FauNDI CATATG 1 cut(s) 14
FbaI TGATCA 1 cut(s) 45
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 2 cut(s) 172, 483
FokI GGATG 3 cut(s) 152, 437, 940
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 2 cut(s) 172, 483
FspBI CTAG 1 cut(s) 711
GlaI GCGC 1 cut(s) 110
GluI GCNGC 2 cut(s) 172, 483
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 4 cut(s) 88, 248, 482, 778
HapII CCGG 1 cut(s) 774
HhaI GCGC 1 cut(s) 111
Hin1II CATG 6 cut(s) 146, 193, 325, 783, 941, 970
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 3 cut(s) 116, 222, 418
HpaII CCGG 1 cut(s) 774
HphI GGTGA 2 cut(s) 633, 692
Hpy166II GTNNAC 2 cut(s) 580, 841
Hpy188I TCNGA 3 cut(s) 566, 663, 768
Hpy188III TCNNGA 6 cut(s) 126, 226, 422, 451, 656, 742
Hpy8I GTNNAC 2 cut(s) 580, 841
HpyAV CCTTC 5 cut(s) 99, 340, 448, 472, 520
HpyCH4III ACNGT 2 cut(s) 384, 847
HpyCH4IV ACGT 2 cut(s) 727, 785
HpyCH4V TGCA 4 cut(s) 27, 674, 707, 783
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 1 cut(s) 563
HpySE526I ACGT 2 cut(s) 727, 785
Hsp92II CATG 6 cut(s) 146, 193, 325, 783, 941, 970
HspAI GCGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 45
Kzo9I GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
LmnI GCTCC 2 cut(s) 504, 755
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 4 cut(s) 151, 415, 673, 959
MaeI CTAG 1 cut(s) 711
MaeII ACGT 2 cut(s) 727, 785
MaeIII GTNAC 3 cut(s) 487, 847, 877
MalI GATC 7 cut(s) 47, 79, 130, 538, 568, 660, 665
MboI GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
MboII GAAGA 2 cut(s) 508, 630
MflI RGATCY 2 cut(s) 128, 566
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 4 cut(s) 30, 232, 292, 407
MlyI GAGTC 1 cut(s) 216
MnlI CCTC 7 cut(s) 30, 201, 277, 303, 352, 439, 726
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 3 cut(s) 99, 288, 927
MspA1I CMGCKG 2 cut(s) 485, 633
MspI CCGG 1 cut(s) 774
MspR9I CCNGG 1 cut(s) 775
Mva1269I GAATGC 1 cut(s) 737
MwoI GCNNNNNNNGC 1 cut(s) 33
NciI CCSGG 1 cut(s) 775
NdeI CATATG 1 cut(s) 14
NdeII GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
NlaIII CATG 6 cut(s) 146, 193, 325, 783, 941, 970
NmuCI GTSAC 2 cut(s) 487, 847
NspI RCATGY 2 cut(s) 193, 970
PaeR7I CTCGAG 1 cut(s) 654
PctI GAATGC 1 cut(s) 737
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 2 cut(s) 116, 418
PkrI GCNGC 2 cut(s) 173, 484
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
Ppu21I YACGTR 1 cut(s) 786
PpuMI RGGWCCY 1 cut(s) 448
PsiI TTATAA 2 cut(s) 401, 891
Psp5II RGGWCCY 1 cut(s) 448
PspPI GGNCC 3 cut(s) 86, 448, 776
PspPPI RGGWCCY 1 cut(s) 448
PstNI CAGNNNCTG 1 cut(s) 421
PsuI RGATCY 2 cut(s) 128, 566
PvuII CAGCTG 1 cut(s) 633
RsaI GTAC 2 cut(s) 198, 788
RsaNI GTAC 2 cut(s) 197, 787
SaqAI TTAA 3 cut(s) 99, 288, 927
SatI GCNGC 2 cut(s) 172, 483
Sau3AI GATC 7 cut(s) 45, 77, 128, 536, 566, 658, 663
Sau96I GGNCC 3 cut(s) 86, 448, 776
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 1 cut(s) 775
SduI GDGCHC 1 cut(s) 342
SfaNI GCATC 4 cut(s) 151, 415, 673, 959
SfcI CTRYAG 2 cut(s) 327, 811
Sfr274I CTCGAG 1 cut(s) 654
SinI GGWCC 1 cut(s) 448
SlaI CTCGAG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 205, 654
SmoI CTYRAG 2 cut(s) 205, 654
Sse9I AATT 4 cut(s) 30, 232, 292, 407
SsiI CCGC 2 cut(s) 169, 483
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 1 cut(s) 773
TaaI ACNGT 2 cut(s) 384, 847
TaiI ACGT 2 cut(s) 730, 788
TaqI TCGA 4 cut(s) 344, 655, 731, 741
TasI AATT 4 cut(s) 30, 232, 292, 407
TatI WGTACW 1 cut(s) 196
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 2 cut(s) 116, 418
Tru1I TTAA 3 cut(s) 99, 288, 927
Tru9I TTAA 3 cut(s) 99, 288, 927
TscAI CASTG 2 cut(s) 852, 913
TseFI GTSAC 2 cut(s) 487, 847
TseI GCWGC 1 cut(s) 171
Tsp45I GTSAC 2 cut(s) 487, 847
TspDTI ATGAA 3 cut(s) 159, 491, 502
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 2 cut(s) 852, 913
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 2 cut(s) 30, 292
XceI RCATGY 2 cut(s) 193, 970
XhoI CTCGAG 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.